RESUMO

Ten captive neotropical Brazilian feline were submitted to gastroscopic examination and samples of gastric mucosa from fundus, corpus and pyloric antrum were evaluated for the presence of Helicobacter species. Warthin-Starry (WS) staining and PCR assay with species-specific primers and enzymatic cleavage were applied for bacterial detection and identification. Histological lesions were evaluated by haematoxylin and eosin staining. All animals showed normal gross aspect of gastric mucosa. Helicobacter heilmannii was confirmed in 100% of the samples by WS and PCR assay. Mild lymphocytic infiltrate in the lamina propria was observed in eight animals, mainly in the fundus region. Small lymphoid follicles were seen in three animals. No significant association between Helicobacter infection and histological findings was verified. These observations suggest that gastric Helicobacter spp. could be a commensal or a eventual pathogen to captive neotropical feline, and that procedures, way life, and stress level on the shelter apparently had no negative repercussion over the integrity of the stomach.

RESUMO

The clinical and the pathological findings associated with Platynosomum fastosum-induced infections are described in Domestic shorthair cats by the retrospective analyses of archival records. Icterus was the most predominant clinical alteration observed; three cats demonstrated clinical manifestations of hepatic encephalopathy. The laboratory findings of two cases confirmed severe hepatic dysfunction due to elevations in the serum concentrations of alanine aminotransferase, phosphatase alkaline, and gamma-glutamyl transferase. Hypertrophy of bile ducts occurred in all animals. All cats demonstrated adenomatous hyperplasia of biliary epithelium and/or periductal fibrosis associated with intraductal trematode and/or intralesional ova of P. fastosum. One cat had pathological alterations consistent with cystic mucinous hyperplasia and gallbladder mucocele associated with intralesional ova of P. fastosum, while dilated cystic intra-hepatic structures associated with chronic platynosomiasis occurred in another cat. Platynosomiasis was an incidental finding in one cat.

RESUMO

A thirteen-year-old female German Spitz dog was referred to Veterinary Hospital of the Universidade Estadual de Londrina, with acute paraplegia and pain in pelvic limbs for two days. The clinical sings, as lack of femoral pulse and cold distal limbs, were consistent with ischemic neuromyopathy due to aortic thromboembolism. Soon after the examination, she had a severe epistaxis and died. On postmortem examination it was observed a thrombus adhered to the aorta trifurcation and a mass occupying the position of the right adrenal gland, which was diagnosed as pheochromocytoma on histological and immunohistochemistry examinations.

RESUMO

Nile tilapias (Oreochromis niloticus)were intraperitoneally infected with a Streptococcus agalactiae suspension containing 105CFU/mL. The strain of S. agalactiae used to experimental infection was isolated from naturally infected tilapias from Paraná, south Brazil. Fishes were kept in laboratory aquaria with adequate limnologic parameters, being euthanized 3, 7, 14, 21 and 28 days post-inoculation. The isolate caused exophtalmia, ocular opacity, erratic swimming, lethargy and darkness of the skin 3, 7 and 14 post-inoculation. Histopathology revealed a septicemia with a severe mononuclear infiltrate in meninges, epicardium and eyes. Inflammation occurred mainly 3 and 7 days post-inoculation, and at 21 and 28 days was not observed. A significant association was verified between infection with S. agalactiae and the presence of melanomacrophages centers in the liver and spleen. S. agalactiae was reisolated from brain and kidney 3, 7 and 14 days post-inoculation.

RESUMO

The aim of this study was to investigate the association between the lesions of the gastric mucosa in slaughter pigs and sex, carcass weight and presence of Helicobacter spp. Stomachs from 236 swine were examined. Gastroesophageal lesions were classified in agreement severity in grade 0, 1, 2 and 3. Fragments from the aglandular and glandular area were processed for histology and for dentification of Helicobacter spp in tissue sections. Macroscopic analysis revealed 203 (86.1%) stomachs with some degree of lesion. Grade 2 and 3 lesions were observed in 104 (44.1%) animals. Grade 3 lesions were observed in 25 (21.1%) castrated males and 20 (16.5%) females. The average carcass weight of animals with grade 0 and 1 lesions and grade 2 and 3 was 82,5 Kg and 79,5 Kg, respectively. One hundred and twelve (47.5%) pigs were positive for Helicobacter spp. by Warthin-Starry stain method ; among them, 54 (48.2%) had grade 2 and 3 lesions; and 58 (51.8%) had grade 0 and 1 lesions. One hundred and twentyfour (52.5%) were negative for Helicobacter spp; among them 50 (40.3%) had grade 2 and 3 lesions, and 74 (59.7%) had grade 0 and 1. There was no significant difference between pigs with and without gastric lesions in regard to the presence of Helicobacter spp, sex and weight.

O objetivo deste estudo foi determinar a ocorrência de lesões gástricas em suínos de abate e verificar a relação das lesões com o gênero, o peso da carcaça e a presença de Helicobacter spp na mucosa gástrica. Foram colhidos e examinados 236 estômagos de suínos. As lesões da pars esophagea e região glandular foram classificadas conforme a severidade em graus 0, 1, 2 e 3. Fragmentos das regiões aglandular e glandular foram processados para exame histológico e para pesquisa de Helicobacter spp. A análise macroscópica revelou que 203 (86.1%) estômagos apresentavam algum tipo de lesão. A ocorrência de erosões e úlceras na região aglandular foi observada em 104 (44.1%) animais e na região glandular em 22 (9.3%). A ulceração na região gastroesofágica estava presente em 45 animais (19.1%), dos quais 25 (21.1%) eram machos e 20 (16.5%) fêmeas. O peso médio da carcaça foi de 82.7 Kg nos animais sem lesões e com paraqueratose na pars esophagea e de 79.5 Kg nos animais com erosões e ulcerações. Utilizando a coloração de Warthin-Starry, observamos Helicobacter spp na mucosa gástrica de 112 (47.5%) amostras. Destas, 54 (48.2%) foram classificadas como grau 2 ou 3 (pars esophagea) e 58 (51.8%) como grau 0 e 1. Dos animais positivos para o Helicobacter spp, 26 (23.2%) apresentavam úlceras na pars esophagea e 24 (21.4%) apresentavam a mucosa sem alterações. Dos 124 (52.5%) animais negativos, 50

RESUMO

Enzootic hematuria is a cronic disease of cows clinically characterized by intermittent hematuria that can be caused by bracken fern (Pteridium aquilinum) and the papilomatosis virus. We describe the clinical and laboratorial abnormalities observed during period of 6 months in naturally affect cows extensively raised in the north region of Paraná State. The animals had low concentrations of total calcium, with normal levels phosphorus and magnesium. Fractional excretion of calcium and phosphorus were increased. Metaplasia and hyperplasia in the epithelium as well as papilomas, hemangiomas, and transitional cell carcinomas were observed histopathologically in the urinary bladder. We were unable to demonstrate any viral presence by imunnohistochemistry and polymerase chain reaction (PCR) of paraffin blocks.

A hematúria enzoótica é uma doença crônica dos bovinos caracterizada clinicamente por hematúria intermitente que pode ser causada pela samambaia (Pteridium aquilinum) e o vírus da papilomatose. Por meio deste estudo verificamos as alterações clínico-laboratoriais em bovinos, mestiços, fêmeas, naturalmente intoxicados pela samambaia, em propriedades rurais de criação extensiva na região norte do Paraná, durante o período de 6 meses. Os resultados mostraram baixo nível plasmático de cálcio, níveis plasmáticos normais para fósforo e magnésio, aumento da excreção urinária fracionada de cálcio e fósforo. Histopatologicamente foram observadas alterações neoplásicas como papiloma, hemangioma e carcinoma de células transicionais além de metaplasia e hiperplasia na bexiga. Provas de imuno-histoquimica e reação em cadeia pela polimerase (PCR) de cortes parafinados mostraram resultado negativo para o agente viral.

RESUMO

The objective of this trial was to detect leptospires in fragments of kidney, liver and uterus of 96 cows with unknown sanitary status, randomly chosen at slaughter in Paraná, Brazil. All 96 urine samples were submitted to direct examination using dark field microscopy. Positive samples in the direct examination and all kidney, liver and uterus fragments were cultured in EMJH modified medium and Tween 80/40/LH. Sections cut of kidney, liver and uterus were stained by Hematoxylin-Eosin (HE) and indirect immunoperoxidase with hyperimmune serum against serovar Hardjo (Hardjoprajitno) and strain Londrina 14 (LO14), from serovar Canicola, which was isolated in the northern region of Paraná state, Brazil, used as primary antibodies. Direct examination detected leptospires in the urine of four animals. All attempts to isolate leptospires from urine and kidney, liver and uterus fragments were negative after 16 weeks of incubation. In the HE stain, focal infiltrate of mononuclear inflammatory cells was observed in the renal interstitial area of most animals. In the indirect immunoperoxidase assay using hyperimmune serum against LO14 strain, the kidney of only one animal presented positive results. All fragments of kidney, liver and uterus tested with hyperimmune serum against serovar Hardjo were negative.

O objetivo deste trabalho foi detectar leptospiras em fragmentos de rim, fígado e útero de 96 fêmeas bovinas com histórico sanitário desconhecido, escolhidas aleatoriamente durante o abate em um frigorífico no Estado do Paraná, Brasil. Todas as 96 amostras de urina foram submetidas ao exame direto em microscópio de campo escuro. As amostras positivas neste exame e todos os fragmentos de rim, fígado e útero foram semeados nos meios de cultura EMJH modificado e Tween 80/40/LH. Os cortes histológicos de rim, fígado e útero foram submetidos à coloração de Hematoxilina-Eosina (HE) e a prova de imunoperoxidase indireta com soros hiperimunes contra o sorovar Hardjo (Hardjoprajitno) e contra a estirpe Londrina (LO14), do sorovar Canicola, isolada no norte do Paraná, Brasil, utilizados como anticorpo primário. O exame direto detectou leptospiras na urina de quatro animais. Todas as tentativas de isolamento das urinas e fragmentos de rim, fígado e útero foram negativas após 16 semanas de incubação. No exame histopatológico foi observado infiltrado focal de células mononucleares no interstício renal da maioria dos animais. Na prova de imunoperoxidase indireta com o soro hiperimune contra a estirpe LO14, o rim de um animal foi o único a apresentar resultado positivo. Todos os fragmentos de rim, fígado e útero testados com o soro hiperimune contra o sorovar Hardjo foram negativos.

RESUMO

Enzootic hematuria is a cronic disease of cows clinically characterized by intermittent hematuria that can be caused by bracken fern (Pteridium aquilinum) and the papilomatosis virus. We describe the clinical and laboratorial abnormalities observed during period of 6 months in naturally affect cows extensively raised in the north region of Paraná State. The animals had low concentrations of total calcium, with normal levels phosphorus and magnesium. Fractional excretion of calcium and phosphorus were increased. Metaplasia and hyperplasia in the epithelium as well as papilomas, hemangiomas, and transitional cell carcinomas were observed histopathologically in the urinary bladder. We were unable to demonstrate any viral presence by imunnohistochemistry and polymerase chain reaction (PCR) of paraffin blocks.

A hematúria enzoótica é uma doença crônica dos bovinos caracterizada clinicamente por hematúria intermitente que pode ser causada pela samambaia (Pteridium aquilinum) e o vírus da papilomatose. Por meio deste estudo verificamos as alterações clínico-laboratoriais em bovinos, mestiços, fêmeas, naturalmente intoxicados pela samambaia, em propriedades rurais de criação extensiva na região norte do Paraná, durante o período de 6 meses. Os resultados mostraram baixo nível plasmático de cálcio, níveis plasmáticos normais para fósforo e magnésio, aumento da excreção urinária fracionada de cálcio e fósforo. Histopatologicamente foram observadas alterações neoplásicas como papiloma, hemangioma e carcinoma de células transicionais além de metaplasia e hiperplasia na bexiga. Provas de imuno-histoquimica e reação em cadeia pela polimerase (PCR) de cortes parafinados mostraram resultado negativo para o agente viral.

RESUMO

The article reviews some of the main characteristics of Pteridium aquilinum (bracken fern) and the main consequences of its ingestion by livestock animals, laboratory animals and man. Ptaquiloside is considered the main responsible for the neoplasias in these cases. Three main routes may lead to human exposure to the toxic effects of bracken fern: eating the plant, physical contact (inhalation of the spores or ingestion of contaminated water) and ingestion of raw milk from affected animals.

O artigo revisa algumas das principais características da planta Pteridium aquilinum (samambaia) bem como as conseqüências da sua ingestão por animais de produção, animais de laboratório e pelo próprio homem. O composto ptaquilosídeo encontrado na planta é considerado atualmente o principal responsável pelo aparecimento de neoplasias. No homem, existem três maneiras pelo qual este pode ser intoxicado: ingestão da planta, contato físico (inalação dos esporos ou ingestão de água contaminada) e ingestão de leite cru de animais que ingeriram a planta.

RESUMO

Swine leptospirosis is recognized world wide as an important cause of reproductive failure. Serum and histo-pathological examinations have been constantly used to diagnose this disease. This study was carried out on slaughtered sows from Northern Paraná State to compare serum findings with kidney, liver, ovary and uterus histo-pathological examinations. The microscopic serum-agglutination carried out on 36 animals detected 24 with titles > 100 and 12 negative ( 100). The histopathological examinations made on livers, kidneys, ovaries and uterus stained with hematoxylin-eosin, were carried out on 16 randomly chosen animals, 11 from the groups with > 100 serological titles and five from the negative group. The results showed that all the 16 animals had indicative lesions in the liver or in one of the kidneys. There were no lesions in the ovaries or uterus. Microscopic serum-agglutination title readings between 20 and 80 were detected in animals of the group considered negative with title 100.

A leptospirose suína é reconhecida mundialmente como uma importante causa de doença reprodutiva. Os exames sorológicos e histopatológicos têm sido constantemente utilizados para o diagnóstico dessa enfermidade. Este trabalho foi realizado em matrizes suínas de descarte da região norte do Estado do Paraná com o objetivo de comparar os achados sorológicos com os exames histopatológicos de rim, fígado, ovário e útero. A soroaglutinação microscópica realizada em 36 animais, detectou 24 com títulos considerados positivos (> 100) e 12 negativos ( 100). Os exames histopatológicos realizados em cortes de fígados, rins, ovários e úteros corados pela hematoxilina-eosina, foram realizados em 16 animais escolhidos aleatoriamente (11 do grupo com título sorológico > 100 e cinco do grupo 100). Os resultados mostraram que todos os 16 animais tinham lesões histológicas sugestivas no fígado ou em um dos rins. Os ovários e úteros examinados não apresentaram lesões. Foram detectados títulos entre 20 a 80 na soroaglutinação microscópica nos cinco animais do grupo com título 100.