RESUMO

For ethical, economic and technical reasons in vivo assays need to be replaced in routine laboratory procedures. Based on a method which is already accepted by the British authorities, an indirect ELISA has been developed and evaluated for Clostridium perfringens type D-containing vaccines. Individual and pooled sera of vaccinated rabbits were tested at a single dilution level, the results transferred into IU/ml, and compared with the conventional toxin neutralization test in mice. The ELISA was found to give reproducible estimates of antitoxin levels and showed good correlation with the conventional in vivo test in mice.

Assuntos

Toxinas Bacterianas/imunologia , Vacinas Bacterianas/normas , Clostridium perfringens/imunologia , Ensaio de Imunoadsorção Enzimática/veterinária , Toxoides/imunologia , Animais , Antígenos de Bactérias/sangue , Antígenos de Bactérias/imunologia , Toxinas Bacterianas/sangue , Vacinas Bacterianas/análise , Calibragem , Testes de Fixação de Complemento , Eletroforese em Gel de Poliacrilamida/veterinária , Camundongos , Coelhos , Padrões de Referência , Reprodutibilidade dos Testes