RESUMO
El presente trabajo intentará reflexionar acerca de la práctica de Interconsulta de Salud Mental, en una sala de pediatría general, dentro del H.I.A.E.P. Sor María Ludovica, ubicado en la ciudad de La Plata, Buenos Aires. Dicha práctica se enmarca como rotación curricular del programa de residentes de psicología y tiene una duración de nueve meses. El objetivo del mismo es tensionar las concepciones de la interconsulta desde el discurso médico y desde el discurso psicoanalítico, en pos de precisar y tender puentes de diálogo e intercambio posibles entre las disciplinas
Assuntos
Serviços de Saúde MentalRESUMO
BACKGROUND: Melatonin has oncostatic actions and IL-25 is active in inflammatory processes that induce apoptosis in tumor cells AIM: The aim of this study was to evaluate melatonin and IL-25 in metastatic (CF-41) and non-metastatic (CMT-U229) canine mammary tumor cells cultured as monolayers and tridimensional structures. MATERIALS AND METHODS: The cells were treated with melatonin, IL-25 and IL-17B silencing gene and performed cell viability, gene and protein expression of caspase-3 and VEGFA (Vascular endothelial growth factor A) and an apoptosis membrane protein array. RESULTS: Treatment with 1 mM of melatonin reduced cell viability of both tumor cell lines, all treatments alone and combined significantly increased caspase-3 cleaved and proteins involved in the apoptotic pathway and reduced pro-angiogenic VEGFA, confirming the effectiveness of these potential promising treatments. CONCLUSION: This is the first study evaluating the potential use of these strategies in CF-41 and CMT-U229 cell lines and together encourages subsequent in vitro and in vivo studies for further exploration of clinical applications.
Assuntos
Apoptose/efeitos dos fármacos , Doenças do Cão/tratamento farmacológico , Interleucina-17/farmacologia , Neoplasias Mamárias Animais/tratamento farmacológico , Melatonina/farmacologia , Animais , Caspase 3/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Doenças do Cão/patologia , Cães , Feminino , Imunofluorescência/veterinária , Inativação Gênica , Neoplasias Mamárias Animais/patologia , Fator A de Crescimento do Endotélio Vascular/metabolismoRESUMO
BACKGROUND: Kimura's disease is a rare benign chronic inflammatory disease of unknown aetiology. CASE REPORT: An 18-year-old atopic Brazilian patient consulted for recurrent facial eczema that improved slightly under topical corticosteroids. He presented a large infiltrated and eczematous subcutaneous plaque on the right supraorbital area, together with eyelid oedema. This was associated with complete right eyebrow alopecia, oedema of the upper lip and infiltrated facial papules. The tests showed hypereosinophilia (4000/mm(3)), a high IgE level (3786 kIU/L) and proteinuria (0.3g/24h). Histological examination revealed a lymphocytic eosinophil-rich inflammatory infiltrate in the superficial and deep dermis, with some lymphoid follicles in depth and proliferation of post-capillary venules. All of these elements led to the diagnosis of Kimura's disease. DISCUSSION: We report an unusual clinical form of Kimura's disease in a Brazilian patient. This disease has been classically described in young Asian men. In our case, a particularly large infiltrated and oedematous subcutaneous plaque was noted. In fact, the more common forms appear as subcutaneous nodules on the head and neck, which may be associated with locoregional adenopathy, involvement of the salivary glands, hypereosinophilia and raised serum IgE. In our case, the skin lesions, characterized by a large infiltrated and oedematous subcutaneous plaque, were unusual and could evoke IgG4-related disease. CONCLUSION: We report a case of Kimura's disease with an atypical presentation. The diagnosis was established by comparing certain histopathological features, and the possibility of IgG4-related disease was discussed.
Assuntos
Hiperplasia Angiolinfoide com Eosinofilia/diagnóstico , Adolescente , Corticosteroides/uso terapêutico , Hiperplasia Angiolinfoide com Eosinofilia/tratamento farmacológico , Hiperplasia Angiolinfoide com Eosinofilia/imunologia , Hiperplasia Angiolinfoide com Eosinofilia/patologia , Brasil/etnologia , Ciclosporina/uso terapêutico , Derme/patologia , Humanos , Imunoglobulina E/sangue , Imunoglobulina G/sangue , Imunofenotipagem , Imunossupressores/uso terapêutico , Masculino , Proteinúria/etiologia , Subpopulações de Linfócitos T/patologiaRESUMO
Breast cancer is one of the leading causes of cancer-related deaths amongst women in the USA. The tumor microenvironment has been suggested to be an attractive therapeutic target for treatment of cancers. The glycosaminoglycan chondroitin sulfate, as part of the cellular microenvironment, consists of long linear chains of repeating disaccharide units, which are covalently attached to core proteins to form chondroitin sulfate-proteoglycans. In vitro studies have implicated chondroitin sulfate in various aspects of carcinogenesis, whereas the in vivo roles of chondroitin sulfate are less clear. Drastically elevated levels of chondroitin sulfate have been observed within the stromal compartment of many solid tumors, including human breast carcinomas, the significance of which is unknown. We examined the role of tumor-associated chondroitin sulfate in breast cancer progression. Enzymatic elimination of endogenous chondroitin sulfate by intra-tumor injections of chondroitinase ABC leads to the development of secondary tumors and increased lung metastases, while primary orthotopic tumor growth was not affected. These results establish a metastasis-inhibiting effect of primary breast tumor-associated chondroitin sulfate, which may open novel carbohydrate-based therapeutic strategies to combat breast cancer.
Assuntos
Sulfatos de Condroitina/metabolismo , Neoplasias Pulmonares/secundário , Neoplasias Mamárias Animais/metabolismo , Neoplasias Mamárias Animais/patologia , Animais , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Condroitina ABC Liase/administração & dosagem , Condroitina ABC Liase/farmacologia , Feminino , Injeções , CamundongosRESUMO
BACKGROUND: There have been few studies in France concerning the specific features of dermatological practice regarding dark skin (Fitzpatrick's phototype V and VI) or the special requirements of black African and Afro-Caribbean patients. AIM: To determine the principal reasons for dermatological consultation among black patients of African or Afro-Caribbean descent in the Paris region. METHODS: This was a prospective clinical study conducted between 15 February and 15 May 2004. The diagnoses of cutaneous conditions leading to dermatological consultation for all black patients of phototype V to VI were recorded by 10 dermatologists practicing in 14 centres within the Paris region. LIMITS: The method used did not allow any conclusions to be drawn regarding the incidence of the presenting conditions among the global population nor did it allow comparison between populations of different phototypes. The absence of any preset list of diagnoses or of precise inclusion criteria regarding evaluation of skin colour left individual investigators with a broad margin of interpretation. RESULTS: In 836 adults and 228 children (half of whom were from Africa and half from the West Indies), diagnoses were as follows: acne in 29.2% of adults and 13.2% in children, and eczema in 6.8% of adults and 27.2% of children. Among dermatoses more specific to black subjects, scalp conditions were frequently seen in both adults (alopecia 7% of diagnoses) and children (tinea capitis 9.6% and alopecia 3.6% of diagnoses). In at least 25% of cases, consultation was associated with dyschromia. Clinical signs suggesting the use of skin lightening products were seen in 95 patients. CONCLUSION: In France, as in other industrialized countries, black patients consult dermatologists essentially for common benign dermatoses also seen amongst white people. Nevertheless, it is important to emphasise the presence of skin problems specific to black patients such as dyschromia and pigmentary disorders, hair and scalp dermatoses, and side effects associated with the use of skin lightening products.
Assuntos
População Negra , Dermatopatias/epidemiologia , Pigmentação da Pele , Acne Vulgar/epidemiologia , Adulto , África/etnologia , Criança , Humanos , Paris , Couro Cabeludo/patologia , Dermatoses do Couro Cabeludo/epidemiologia , Dermatopatias/classificação , Índias Ocidentais/etnologiaRESUMO
Like other types of pre-malignant lesions and carcinoma, angiogenesis is associated with high-grade cervical dysplasia and with invasive squamous carcinoma of the cervix. Vascular endothelial cell growth factor (VEGF) is known to be one of the most important inducers of angiogenesis and is upregulated in carcinoma of the cervix. Human Papilloma Virus 16 (HPV-16) has been etiologically linked to human cervical cancer, and the major oncogenic proteins encoded by the viral genome, E6 and E7, are involved in the immortalization of target cells. Because several oncogenes including mutant ras, EGF receptor, ErbB2/Her2, c-myc and v-src upregulate VEGF expression, we asked whether HVP-16 E6 oncoprotein could act in a similar fashion. We found that HPV-16 E6-positive cells generally express high levels of VEGF message. Furthermore, co-expression of the VEGF promoter-Luc (luciferase) reporter gene with E6 in both human keratinocytes and mouse fibroblast showed that E6 oncoprotein upregulates VEGF promoter activity, and does so in a p53 independent manner. An E6 responsive region which comprises four Sp-1 sites, between -194 and -50 bp of the VEGF promoter, is also necessary for constitutive VEGF transcription. Taken together, our results suggest the possibility that the HPV oncoprotein E6 may contribute to tumor angiogenesis by direct stimulation of the VEGF gene.
Assuntos
Fatores de Crescimento Endotelial/genética , Genes p53 , Linfocinas/genética , Neovascularização Patológica/genética , Proteínas Oncogênicas Virais/fisiologia , Papillomaviridae/genética , Regiões Promotoras Genéticas , Proteínas Repressoras , Proteína Supressora de Tumor p53/fisiologia , Comunicação Autócrina , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/virologia , Fatores de Crescimento Endotelial/metabolismo , Receptores ErbB/fisiologia , Feminino , Células HeLa/metabolismo , Células HeLa/virologia , Humanos , Queratinócitos/virologia , Linfocinas/metabolismo , Proteínas de Neoplasias/fisiologia , Neovascularização Patológica/fisiopatologia , Proteínas Oncogênicas Virais/genética , Papillomaviridae/fisiologia , Infecções por Papillomavirus/patologia , Infecções por Papillomavirus/virologia , Proteínas Recombinantes de Fusão/biossíntese , Transcrição Gênica , Ativação Transcricional , Fator de Crescimento Transformador alfa/fisiologia , Células Tumorais Cultivadas/metabolismo , Células Tumorais Cultivadas/virologia , Infecções Tumorais por Vírus/patologia , Infecções Tumorais por Vírus/virologia , Neoplasias do Colo do Útero/metabolismo , Neoplasias do Colo do Útero/patologia , Neoplasias do Colo do Útero/virologia , Fator A de Crescimento do Endotélio Vascular , Fatores de Crescimento do Endotélio Vascular , Neoplasias Vulvares/metabolismo , Neoplasias Vulvares/patologia , Neoplasias Vulvares/virologiaRESUMO
We have introduced the maize Ac transposable element in Arabidopsis thaliana and found that after three selfing generations, the element is immobile and extensively methylated. Moreover, the nopaline synthase (nos) gene present on the same transferred T-DNA, was active early after transformation and regeneration, but inactive in most of the S1 progeny. We used 5-azacytidine (5AzaC) to determine whether a reduction in the methylation would affect both Ac transposition and expression of the nos gene. After treatment with 5AzaC doses from 0.3 mM to 1.0 mM, approximately 25% of the plants produced detectable amounts of nopaline, indicating that the nos gene was reactivated. Using the polymerase chain reaction (PCR) to detect the empty donor site left by Ac transposition, we demonstrated that 5AzaC also activates Ac excision in the transgenic plants. Approximately 13% of the 5AzaC treated plants (doses from 0.1 mM to 1.0 mM) were shown to have empty donor sites due to Ac excision. None of the plants cultivated in the absence of 5AzaC showed evidence for Ac transposition or reactivation of the nos gene. Further analysis using Southern blot indicate that some demethylation occurred in the genome of individual plants. These results may represent demethylation in few cells during development which may be sufficient to reactivate in these cells the expression of the nos and Ac transposase transgenes, the latter promoting Ac transposition in somatic cells.
Assuntos
Arabidopsis/genética , Azacitidina/farmacologia , Elementos de DNA Transponíveis , Aminoácido Oxirredutases/biossíntese , Aminoácido Oxirredutases/genética , Arabidopsis/efeitos dos fármacos , Arabidopsis/enzimologia , Sequência de Bases , Metilação de DNA , Primers do DNA , DNA Bacteriano , DNA de Plantas/química , Deleção de Genes , Plantas Geneticamente Modificadas , Plasmídeos , Reação em Cadeia da Polimerase , Proteínas Recombinantes/biossíntese , SementesRESUMO
Se revisaron 97.480 fichas de los archivos del Instituto Hematológico de Occidente, estado Zulia, Venezuela, procedentes de los diferentes distritos con el fin de realizar un estudio sobre Hepatitis B en donantes de sangre por un período de 9 años (1983-1991). El método serológico utilizado fue el inmunoenzimático Corzyme, Abbott Laboratorio, obteniéndose 2.136 casos positivos (2,19 por ciento). A partir del año 1987 se observa un incremento del número de casos de Hepatitis B, lo cual se explica por la inclusión del anticuerpo core (AccHB) en conjunto por el antígeno de superficie (AgsHB) como marcador diagnóstico de la referida patología. De los casos positivos de Hepatitis B el mayor porcentaje corresponde al anticuerpo core (AccHB) (54,54 por ciento), seguido en frecuencia por el antígeno de superficie (AgsHB) (41,39 por ciento) y ambos marcadores (AgsHB + AccHB) (4,07 por ciento)