Assuntos
Ataxia Telangiectasia/diagnóstico , Linfoma de Burkitt/diagnóstico , Proteínas Proto-Oncogênicas c-myc/metabolismo , Ataxia Telangiectasia/complicações , Ataxia Telangiectasia/genética , Linfoma de Burkitt/complicações , Linfoma de Burkitt/genética , Criança , Cromossomos Humanos Par 8 , Humanos , Hibridização in Situ Fluorescente , Cariótipo , Masculino , Proteínas Proto-Oncogênicas c-myc/genética , Translocação Genética , TrissomiaRESUMO
Some proteins, canonically not associated with amyloid diseases, can aggregate into amyloid-like fibrils under special conditions. Our group hypothesized that stressful cancer microenvironment might induce the formation of insoluble deposits of p53 mutant protein. A cohort of 28 non-small cell lung cancer (NSCLC) patients was used to test the aforementioned hypothesis. Tumor specimens were assessed for TP53 mutations using DNA sequencing and for amyloid formation by Congo red staining. TP53 mutations were present in 57% of patients, whereas no amyloid deposits were detected in tissue sections under polarized light microscopy. Mutant p53 proteins are not associated with the appearance of amyloid-like fibrils in NSCLC samples, and DNA sequencing remains the standard method to detect such abnormality.
Assuntos
Amiloide/genética , Carcinoma Pulmonar de Células não Pequenas/patologia , Neoplasias Pulmonares/patologia , Proteínas Mutantes/genética , Mutação/genética , Proteína Supressora de Tumor p53/genética , Adenocarcinoma/genética , Adenocarcinoma/patologia , Adulto , Idoso , Carcinoma de Células Grandes/genética , Carcinoma de Células Grandes/patologia , Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , DNA de Neoplasias/genética , Feminino , Humanos , Neoplasias Pulmonares/genética , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Reação em Cadeia da Polimerase , PrognósticoRESUMO
A spontaneous adenocarcinoma occurred in one (0.22%) adult male Syrian hamster out of 443 necropsied from a breeding animal house of the state of Rio de Janeiro, Brazil. Gross inspection showed that the renal neoplasm consisted of a unilateral single white nodule of hard consistency located in the cortex, measuring 5 mm in diameter. Microscopically, the renal epithelial cells were markedly pleomorphic and presented an abundant granular eosinophilic cytoplasm. Cells were cuboidal and mainly formed trabeculae of various widths without a lumen and also formed solid areas. There were necrotic areas and three mitotic figures per 30 high-power fields. No intravascular invasion or metastasis was observed. Positive expression of vimentin and negative expression of cytokeratin 7, pan-cytokeratin and CD10 were observed in the tumor. The histopathology and immunohistochemical profile of the renal tumor suggested the diagnosis of the eosinophilic variant of adenocarcinoma, which was reported for the first time in this species.
Assuntos
Animais , Granuloma Eosinófilo/diagnóstico , Granuloma Eosinófilo/mortalidade , Granuloma Eosinófilo/veterinária , Vimentina/análise , Vimentina/biossínteseRESUMO
A spontaneous adenocarcinoma occurred in one (0.22%) adult male Syrian hamster out of 443 necropsied from a breeding animal house of the state of Rio de Janeiro, Brazil. Gross inspection showed that the renal neoplasm consisted of a unilateral single white nodule of hard consistency located in the cortex, measuring 5 mm in diameter. Microscopically, the renal epithelial cells were markedly pleomorphic and presented an abundant granular eosinophilic cytoplasm. Cells were cuboidal and mainly formed trabeculae of various widths without a lumen and also formed solid areas. There were necrotic areas and three mitotic figures per 30 high-power fields. No intravascular invasion or metastasis was observed. Positive expression of vimentin and negative expression of cytokeratin 7, pan-cytokeratin and CD10 were observed in the tumor. The histopathology and immunohistochemical profile of the renal tumor suggested the diagnosis of the eosinophilic variant of adenocarcinoma, which was reported for the first time in this species.(AU)