RESUMO
This study aimed to evaluate the effects of different temperatures on incubation variables, performance, and morphometry of the duodenal mucosa of Japanese quails (Coturnix coturnix japonica) submitted to chronic heat stress after hatching. We distributed 540 eggs in three incubators with a temperature of 37.8°C and 60% of humidity. From the 6th day of incubation until hatching, the temperatures were adjusted to (37.8°C, 38.5°C and 39.5°C). After hatching, quails were evaluated for the quality score, weighed, and distributed in a completely randomized design with three incubation temperatures (37.8, 38.5, and 39.5°C) and two ambient temperatures (stress and thermoneutral). At 10, 20, 30, and 40 days they were weighed to determine the live weight (g) and weight gain(g). To collect the duodenum and determine morphometric parameters, we euthanized four quails of each treatment. The data were analyzed, and the differences between the means determined by the Tukey test at 5%. The incubation temperature of 39.5°C provided lower hatching rate and the live weight at birth; however, from the 10th day of age, increased live weight, weight gain, and positively influenced the morphological parameters of the duodenal mucosa in situations of chronic stress.(AU)
Objetivou-se avaliar os efeitos de diferentes temperaturas de incubação sobre as variáveis de incubação, desempenho e morfometria da mucosa duodenal de codornas japonesas (Coturnix coturnix japonica) submetidas ao estresse térmico crônico por calor após eclosão. Foram distribuídos 540 ovos em três incubadoras, com temperatura de 37,8°C e umidade 60%. A partir do sexto dia de incubação até a eclosão, as temperaturas foram ajustadas para 37,8°C, 38,5°C e 39,5°C. Após a eclosão, as codornas foram avaliadas quanto ao escore de qualidade, pesadas e distribuídas em um delineamento inteiramente ao acaso, com três temperaturas de incubação (37,8ºC, 38,5ºC e 39,5°C) e duas temperaturas ambientes (estresse e termoneutro). Aos 10, 20, 30 e 40 dias, foram pesadas para determinar o peso vivo (g) e o ganho de peso(g). Quatro codornas de cada tratamento foram eutanasiadas para coleta do duodeno, para determinar os parâmetros morfométricos. Os dados foram analisados e as diferenças entre as médias foram determinadas pelo teste de Tukey a 5%. A temperatura de incubação de 39,5°C proporcionou menor taxa de eclosão e menor peso vivo ao nascer, entretanto, a partir do 10° dia de idade, essa temperatura aumentou o peso vivo, o ganho de peso e influenciou positivamente os parâmetros morfológicos da mucosa duodenal em situações de estresse crônico.(AU)
Assuntos
Animais , Aumento de Peso , Resposta ao Choque Térmico/fisiologia , Coturnix/anatomia & histologia , Duodeno/anatomia & histologia , Duodeno/fisiologia , Incubadoras/veterináriaRESUMO
Objetivou-se avaliar os efeitos da manipulação da temperatura de incubação sobre a resposta imune de codornas desafiadas termicamente após eclosão. Para isso, foram utilizados 540 ovos, distribuídos em três incubadoras, com temperatura de 37,8°C e umidade de 60%. A partir do sexto dia de incubação até a eclosão, as temperaturas foram ajustadas em 37,8°C (padrão), 38,5°C (intermediária) e 39,5°C (alta). Após a eclosão as codornas foram pesadas e distribuídas, em delineamento inteiramente ao acaso, com três temperaturas de incubação (37,8, 38,5 e 39,5°C) e duas temperaturas de ambiente (estresse e termoneutro). Aos 10, 20, 30 e 40 dias, quatro codornas por tratamento foram eutanasiadas para coleta da bolsa cloacal, do fígado e do coração, para se determinar o peso absoluto (g), o peso relativo (%) e a área dos folículos bursais. Sangue foi coletado para realização do hemograma, do leucograma e da bioquímica sérica. Os dados foram analisados e as diferenças entre as médias foram determinadas pelo teste de Tukey a 5%. O estresse térmico por calor, a partir dos 20 dias, promove redução no peso absoluto do fígado, do coração, da bolsa cloacal e na área dos folículos bursais, além de heterofilia, linfopenia e aumento da relação heterófilo/linfócito. Em conclusão, o estresse térmico por calor após 10 dias de idade pode causar imunossupressão.(AU)
The objective of this study was to evaluate the effects of manipulation of the incubation temperature on the immune response of quails challenged thermally after hatching. For this, 540 eggs were distributed in three incubators, with temperature of 37.8°C and 60% humidity. From the 6th day of incubation to hatching the temperatures were adjusted to 37.8°C (standard), 38.5°C (intermediate) and 39.5°C (high). After hatching the quails were weighed and distributed in a completely randomized design with three incubation temperatures (37.8, 38.5 and 39.5°C) and two ambient temperatures (stress and thermoneutral). At 10, 20, 30 and 40 days four quail per treatment were euthanized to collect the cloacal burse, liver and heart to determine the absolute weight (g), relative weight (%) and area of the bursal follicles. Blood was sampled for determination of hemogram, leukogram and serum biochemistry. The data were analyzed and the differences between the means were determined by the Tukey test at 5%. Heat stress from 20 days onwards promotes a reduction in the absolute weight of the liver, heart, cloacal sac and in the area of the follicles. In addition, there was heterofilia, lymphopenia and increased heterophile/lymphocyte ratio. In conclusion, heat stress after 10 days of age can cause immunosuppression.(AU)
Assuntos
Animais , Cloaca/fisiologia , Transtornos de Estresse por Calor/veterinária , Coturnix/fisiologia , Temperatura Alta , Tolerância Imunológica , Incubadoras , Contagem de Leucócitos/veterináriaRESUMO
Objetivou-se avaliar os efeitos da manipulação da temperatura de incubação sobre a resposta imune de codornas desafiadas termicamente após eclosão. Para isso, foram utilizados 540 ovos, distribuídos em três incubadoras, com temperatura de 37,8°C e umidade de 60%. A partir do sexto dia de incubação até a eclosão, as temperaturas foram ajustadas em 37,8°C (padrão), 38,5°C (intermediária) e 39,5°C (alta). Após a eclosão as codornas foram pesadas e distribuídas, em delineamento inteiramente ao acaso, com três temperaturas de incubação (37,8, 38,5 e 39,5°C) e duas temperaturas de ambiente (estresse e termoneutro). Aos 10, 20, 30 e 40 dias, quatro codornas por tratamento foram eutanasiadas para coleta da bolsa cloacal, do fígado e do coração, para se determinar o peso absoluto (g), o peso relativo (%) e a área dos folículos bursais. Sangue foi coletado para realização do hemograma, do leucograma e da bioquímica sérica. Os dados foram analisados e as diferenças entre as médias foram determinadas pelo teste de Tukey a 5%. O estresse térmico por calor, a partir dos 20 dias, promove redução no peso absoluto do fígado, do coração, da bolsa cloacal e na área dos folículos bursais, além de heterofilia, linfopenia e aumento da relação heterófilo/linfócito. Em conclusão, o estresse térmico por calor após 10 dias de idade pode causar imunossupressão.(AU)
The objective of this study was to evaluate the effects of manipulation of the incubation temperature on the immune response of quails challenged thermally after hatching. For this, 540 eggs were distributed in three incubators, with temperature of 37.8°C and 60% humidity. From the 6th day of incubation to hatching the temperatures were adjusted to 37.8°C (standard), 38.5°C (intermediate) and 39.5°C (high). After hatching the quails were weighed and distributed in a completely randomized design with three incubation temperatures (37.8, 38.5 and 39.5°C) and two ambient temperatures (stress and thermoneutral). At 10, 20, 30 and 40 days four quail per treatment were euthanized to collect the cloacal burse, liver and heart to determine the absolute weight (g), relative weight (%) and area of the bursal follicles. Blood was sampled for determination of hemogram, leukogram and serum biochemistry. The data were analyzed and the differences between the means were determined by the Tukey test at 5%. Heat stress from 20 days onwards promotes a reduction in the absolute weight of the liver, heart, cloacal sac and in the area of the follicles. In addition, there was heterofilia, lymphopenia and increased heterophile/lymphocyte ratio. In conclusion, heat stress after 10 days of age can cause immunosuppression.(AU)
Assuntos
Animais , Cloaca/fisiologia , Transtornos de Estresse por Calor/veterinária , Coturnix/fisiologia , Temperatura Alta , Tolerância Imunológica , Incubadoras , Contagem de Leucócitos/veterináriaRESUMO
Avaliou-se o emprego de silagem ácida de vísceras de tambaqui para alimentação de cães jovens. As variáveis estudadas foram o estado geral, o ganho de peso vivo, o escore corporal e fecal, a consistência e odor das fezes e a função hepática e renal dos animais. A ração foi composta com 46% de silagem e calculada para gerar níveis nutricionais iguais aos registrados no rótulo da ração comercial para filhotes e compatível com a recomendação para a idade dos animais, ou seja, 26% PB, de 1,0 a 2,0% de Cálcio, 0,9% de Fósforo e 3295 kcal kg-1 EM. O período experimental foi de 52 dias sendo que as amostras de sangue, para avaliação bioquímica, foram coletas no 53º dia após o jejum de 12 horas. O uso da silagem na alimentação dos cães foi satisfatório não diferindo em relação a todas as variáveis estudadas, em relação ao grupo controle. Este estudo, de caráter exploratório, gera o indicativo de uso da silagem de resíduos de peixes a ser empregado como fonte protéica na formulação de ração para cães.
The aim of this study was to evaluate the use of silage viscera of tambaqui for feeding young dogs. The variables studied were the general condition, live weight gain, body and fecal score, consistency and odor of feces and liver and kidney biochemical functions of animals. The ration consisted of 46% silage and calculated to generate nutrient levels equal to those recorded on the label of the commercial feed and compatible with the recommendation for the age of the animals (26% crude protein, 1.0 to 2.0% calcium, 0.9% phosphorus and 3295 kcal kg-1). The experimental period was 52 days and that the blood samples for biochemical analyzes were collected on day 53 after fasting for 12 hours. The use of silage in the diet of dogs was satisfactory and did not differ with respect to all variables in the control group. This study, exploratory, generates the indicative use of silage from fish waste to be used as a protein source in the diet formulation for dogs.
Assuntos
Animais , Cães , Characidae , Dieta Rica em Proteínas/veterinária , Ração Animal/análise , Resíduos de Alimentos , Silagem/análise , Análise Química do Sangue/veterináriaRESUMO
Avaliou-se o emprego de silagem ácida de vísceras de tambaqui para alimentação de cães jovens. As variáveis estudadas foram o estado geral, o ganho de peso vivo, o escore corporal e fecal, a consistência e odor das fezes e a função hepática e renal dos animais. A ração foi composta com 46% de silagem e calculada para gerar níveis nutricionais iguais aos registrados no rótulo da ração comercial para filhotes e compatível com a recomendação para a idade dos animais, ou seja, 26% PB, de 1,0 a 2,0% de Cálcio, 0,9% de Fósforo e 3295 kcal kg-1 EM. O período experimental foi de 52 dias sendo que as amostras de sangue, para avaliação bioquímica, foram coletas no 53º dia após o jejum de 12 horas. O uso da silagem na alimentação dos cães foi satisfatório não diferindo em relação a todas as variáveis estudadas, em relação ao grupo controle. Este estudo, de caráter exploratório, gera o indicativo de uso da silagem de resíduos de peixes a ser empregado como fonte protéica na formulação de ração para cães.
RESUMO
Avaliou-se o emprego de silagem ácida de vísceras de tambaqui para alimentação de cães jovens. As variáveis estudadas foram o estado geral, o ganho de peso vivo, o escore corporal e fecal, a consistência e odor das fezes e a função hepática e renal dos animais. A ração foi composta com 46% de silagem e calculada para gerar níveis nutricionais iguais aos registrados no rótulo da ração comercial para filhotes e compatível com a recomendação para a idade dos animais, ou seja, 26% PB, de 1,0 a 2,0% de Cálcio, 0,9% de Fósforo e 3295 kcal kg-1 EM. O período experimental foi de 52 dias sendo que as amostras de sangue, para avaliação bioquímica, foram coletas no 53º dia após o jejum de 12 horas. O uso da silagem na alimentação dos cães foi satisfatório não diferindo em relação a todas as variáveis estudadas, em relação ao grupo controle. Este estudo, de caráter exploratório, gera o indicativo de uso da silagem de resíduos de peixes a ser empregado como fonte protéica na formulação de ração para cães.(AU)
The aim of this study was to evaluate the use of silage viscera of tambaqui for feeding young dogs. The variables studied were the general condition, live weight gain, body and fecal score, consistency and odor of feces and liver and kidney biochemical functions of animals. The ration consisted of 46% silage and calculated to generate nutrient levels equal to those recorded on the label of the commercial feed and compatible with the recommendation for the age of the animals (26% crude protein, 1.0 to 2.0% calcium, 0.9% phosphorus and 3295 kcal kg-1). The experimental period was 52 days and that the blood samples for biochemical analyzes were collected on day 53 after fasting for 12 hours. The use of silage in the diet of dogs was satisfactory and did not differ with respect to all variables in the control group. This study, exploratory, generates the indicative use of silage from fish waste to be used as a protein source in the diet formulation for dogs.(AU)
Assuntos
Animais , Cães , Ração Animal/análise , Characidae , Silagem/análise , Resíduos de Alimentos , Dieta Rica em Proteínas/veterinária , Análise Química do Sangue/veterináriaRESUMO
Obesity is characterized by increased adipose tissue mass resulting from a chronic imbalance between energy intake and expenditure. Furthermore, there is a clearly defined relationship among fat mass expansion, chronic low-grade systemic inflammation and reactive oxygen species (ROS) generation; leading to ROS-related pathological events. In the past years, genome-wide association studies have generated convincing evidence associating genetic variation at multiple regions of the genome with traits that reflect obesity. Therefore, the present study aimed to evaluate the relationships among the gene polymorphisms ghrelin (GHRL-rs26802), ghrelin receptor (GHSR-rs572169), leptin (LEP-rs7799039), leptin receptor (LEPR-rs1137101) and fat mass and obesity-associated (FTO-rs9939609) and obesity. The relationships among these gene variants and the amount of DNA damage were also investigated. Three hundred Caucasian morbidly obese and 300 eutrophic (controls) women were recruited. In summary, the results demonstrated that the frequencies of the GHRL, GHSR, LEP and LEPR polymorphisms were not different between Brazilian white morbidly obese and eutrophic women. Exceptions were the AA-FTO genotype and allele A, which were significantly more frequent in obese women than in the controls (0.23% vs. 0.10%; 0.46 vs. 0.36, respectively), and the TT-FTO genotype and the T allele, which were less frequent in morbidly obese women (p<0.01). Furthermore, significant differences in the amount of genetic lesions associated with FTO variants were observed only in obese women. In conclusion, this study demonstrated that the analyzed SNPs were not closely associated with morbid obesity, suggesting they are not the major contributors to obesity. Therefore, our data indicated that these gene variants are not good biomarkers for predicting risk susceptibility for obesity, whereas ROS generated by the inflammatory status might be one of the causes of DNA damage in obese women, favoring genetically related diseases as obesity comorbidities.
Assuntos
Dano ao DNA , Obesidade Mórbida/genética , Polimorfismo de Nucleotídeo Único , Adulto , Dioxigenase FTO Dependente de alfa-Cetoglutarato , Feminino , Grelina/genética , Humanos , Leptina/genética , Obesidade Mórbida/patologia , Proteínas/genética , Receptores de Grelina/genética , Receptores para Leptina/genéticaRESUMO
BACKGROUND: Post-inflammatory hyperpigmentation is a frequent concern when treating solar lentigines. OBJECTIVES: To assess the safety and efficacy of a triple combination cream with fluocinolone acetonide 0.01%, hydroquinone 4% and tretinoin 0.05% as adjuvant to cryotherapy in the treatment of solar lentigines in hands dorsum, and in the prevention of post-inflammatory hyperpigmentation after cryotherapy. METHODS: This prospective, randomized, controlled, investigator-blinded, single-centre study enrolled 50 patients. Twenty-five patients received a 2-week daily triple combination cream plus sunscreen pre-treatment and 25 received sunscreen alone. After that, cryotherapy was performed in all patients followed by a 3-week recovery period. After this period, patients received the same initial treatment and were followed up for 8 weeks. Melanin and erythema levels of a target and a control lentigo were objectively measured using a narrowband reflectance spectrophotometer. Lentigines count, colour homogeneity and global improvement were also assessed. RESULTS: The number of solar lentigines reduced in the first 2 weeks only in patients who used the triple combination 25 ± 7 vs. 22 ± 8 (P < 0.0001), and reduced at the end of the study for both groups (P < 0.0001). The melanin levels also reduced in the first 2 weeks only in patients who used the triple combination 297 ± 69 vs. 273 ± 66 (P < 0.0001) and reduced at the end of the study for both groups (P < 0.0001). Erythema and residual blisters from cryotherapy were the reported adverse reactions. CONCLUSION: Triple combination cream can be used to enhance the resolution of solar lentigines, and to significantly reduce melanin levels and lentigines count, improving treatment results. It was well-tolerated and did not increase the occurrence of neither erythema nor other side-effects after the cryotherapy.
Assuntos
Crioterapia , Dermatoses da Mão/terapia , Lentigo/terapia , Creme para a Pele/uso terapêutico , Anti-Inflamatórios/uso terapêutico , Antineoplásicos/uso terapêutico , Antioxidantes/uso terapêutico , Quimioterapia Adjuvante/efeitos adversos , Crioterapia/efeitos adversos , Combinação de Medicamentos , Eritema/etiologia , Feminino , Fluocinolona Acetonida/uso terapêutico , Dermatoses da Mão/etiologia , Humanos , Hidroquinonas/uso terapêutico , Lentigo/etiologia , Lentigo/metabolismo , Masculino , Melaninas/metabolismo , Pessoa de Meia-Idade , Estudos Prospectivos , Método Simples-Cego , Creme para a Pele/efeitos adversos , Luz Solar/efeitos adversos , Tretinoína/uso terapêuticoRESUMO
The objective of this study was to evaluate the effects of 1% dietary glutamic acid on the body weight, intestinal morphometry, and anti-Newcastle antibody titers of broiler chickens submitted to heat stress. One-d-old male broiler chicks (n=120) were distributed according to a 2 x 2 factorial design with two environmental temperatures (thermoneutral or heat stress) and two diets (with 0 or 1% glutamic acid). Heat stress temperature was constantly maintained (24h/day) 5 ºC higher than the thermoneutral temperature. Diets supplied the nutritional requirements of broilers in the pre-starter (1 to 7d) and starter (8 to 21d) phases. Birds were vaccinated against Newcastle disease on d 7 via eye drop. On days 5, 10, 15, and 20, individual body weight was determined, serum samples were collected from five birds, and duodenum samples were collected from four birds per treatment. Serum anti-Newcastle antibody titers were determined by enzyme immunoassay and transformed into log10. Villus height, crypt depth, and villus: crypt ratio were measured in the duodenum. Data were analyzed by ANOVA. Chronic heat stress negatively affected body weight and intestinal morphometry during the pre-starter and starter phases, but had no effect on antibody titers. Dietary glutamic acid supplementation (1%) improved body weight and intestinal integrity of birds submitted to heat stress when compared with non-supplemented and heat-stressed birds.
Assuntos
Animais , Galinhas/classificação , Galinhas/crescimento & desenvolvimento , Ácido Glutâmico/administração & dosagem , Ácido Glutâmico/análise , Aumento de Peso , Transtornos de Estresse por Calor/classificação , Transtornos de Estresse por Calor/veterináriaRESUMO
The objective of this study was to evaluate the effects of 1% dietary glutamic acid on the body weight, intestinal morphometry, and anti-Newcastle antibody titers of broiler chickens submitted to heat stress. One-d-old male broiler chicks (n=120) were distributed according to a 2 x 2 factorial design with two environmental temperatures (thermoneutral or heat stress) and two diets (with 0 or 1% glutamic acid). Heat stress temperature was constantly maintained (24h/day) 5 ºC higher than the thermoneutral temperature. Diets supplied the nutritional requirements of broilers in the pre-starter (1 to 7d) and starter (8 to 21d) phases. Birds were vaccinated against Newcastle disease on d 7 via eye drop. On days 5, 10, 15, and 20, individual body weight was determined, serum samples were collected from five birds, and duodenum samples were collected from four birds per treatment. Serum anti-Newcastle antibody titers were determined by enzyme immunoassay and transformed into log10. Villus height, crypt depth, and villus: crypt ratio were measured in the duodenum. Data were analyzed by ANOVA. Chronic heat stress negatively affected body weight and intestinal morphometry during the pre-starter and starter phases, but had no effect on antibody titers. Dietary glutamic acid supplementation (1%) improved body weight and intestinal integrity of birds submitted to heat stress when compared with non-supplemented and heat-stressed birds.(AU)