RESUMO
En el presente trabajo se compararon los métodos de aglutinación con partículas de látex (AL) y Rantz-Randall (RR) para investigar anticuerpos antiestreptolisina "O" en 72 muestras de sueros sanguíneos. Este estudio reveló un mayor porcentaje de positividad, por el método RR (55,5 por ciento) que por AL (16,6 por ciento). Cuando evaluamos la discordancia en nuestros datos, se observa que un suero positivo por AL (título 400 UI/ml) fue negativo para RR, lo cual pudo ser un falso positivo por la presencia de anticuerpos inespecíficos. En el caso de las 19 muestras de suero que resultaron positivas por RR a diluciones superiores a 1:240 y negativas por AL, podría explicarse por la mayor sensibilidad de la primera técnica y/o la presencia de falsos negativos por la técnica de AL, debido al fenómeno de prozona. El análisis estadístico mediante el Chi cuadrado, determinó que entre los métodos de RR y AL, hubo diferencia significante, lo cual reveló la importancia de este estudio ya que demuestra que el método de RR constituye una alternativa superior al de AL, como complemento del diagnóstico clínico y bacteriológico de las infecciones estreptocócicas
Assuntos
Humanos , Masculino , Feminino , Infecções Estreptocócicas/diagnóstico , Testes de Fixação do Látex , Medicina Tropical , VenezuelaRESUMO
En el presente trabajo se compararon los métodos de Hemaglutinación Indirecta (HAI) e Inmunoanálisis Enzimático (ELISA, IgM e IgG) para investigar anticuerpos antitoxoplasma en 54 muestras sanguíneas. Los resultados obtenidos muestran una concordancia del 62.96 por ciento entre HAI y ELISA. IgM y del 83.33 por ciento entre HAI y ELISA. IgG. El análisis estadístico mediante el chi cuadrado, reveló que hubo significancia cuando se compararon todos los títulos de HAI a partir de 1:2 con todos los índices de ELISA. IgM, esta significancia no puede establecer la superioridad de un método sobre el otro, debido: a. La utilización del "kit" comercial ELISA. IgM que solo detecta este tipo de anticuerpo; b. La detección por HAI de IgM e IgG; c. La presencia de anticuerpos inespecíficos de HAI y ELISA. IgG reveló que no hubo diferencias, lo cual nos indica que la discordancia entre ambos métodos no es estadísticamente significante
Assuntos
Humanos , Hemaglutinação/imunologia , Toxoplasmose Congênita/genética , Toxoplasmose/patologiaRESUMO
Thirty-one patients with acute pancreatitis were prospectively evaluated using the Early Prognostic Risk Factors of Ranson and Bank & Wise, in order to assess severity of that condition. The purpose was to determine the usefulness of such factors as clues to orient diagnosis and treatment of acute pancreatitis, particularly in the most severe clinical forms; and the factibility of using them in our setting. The presence of 4 or more of Ranson's and/or 1 or more of Bank & Wise Factors fulfilling positive criteria, identified the patients with a high risk of complications and mortality. Hypocalcemia, leucocytosis and hyperuricemia were the most frequent findings in this group. The development of Early Objective Prognostic Risk Factors in acute pancreatitis, as a clinical concept, has improved the management of these patients. It allows to establish diagnosis and therapeutics upon objective basis.
Assuntos
Pancreatite/complicações , Pancreatite/epidemiologia , Doença Aguda , Humanos , Pancreatite/diagnóstico , Peru , Prognóstico , Estudos Prospectivos , Fatores de Risco , Fatores de TempoRESUMO
Gonadotropin serum levels and pulsatile secretion of gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) are regulated by sexual steroids and perhaps inhibin, but the relative rates of LH and follicle-stimulating hormone (FSH) secretion are modulated by the frequency of GnRH pulses. This study evaluated LH pulsatility in patients with idiopathic normogonadotropic oligospermia (INO) and normal men before and after clomiphene citrate (CC) administration. INO patients evidenced a lower mean LH levels (P less than 0.001), a higher mean pulse frequency (P less than 0.05) and similar pulse amplitude than normal men. CC induced in normal men a higher LH and testosterone (T) increments and increased pulse amplitude only in normal men. Estradiol (E2) showed no difference in either group. Patients with INO might evidence a hypothalamic disorder that may alter pulsatile GnRH secretion. A different response to CC in patients with INO seems to lend support to a primary hypothalamic lesion. A probable gonadotropin imbalance might alter intratesticular concentrations of T and E2 and be the cause of spermatogenic failure.
Assuntos
Hormônio Luteinizante/sangue , Oligospermia/sangue , Adulto , Gonadotropina Coriônica/farmacologia , Clomifeno/farmacologia , Estradiol/sangue , Hormônio Foliculoestimulante/sangue , Humanos , Masculino , Oligospermia/tratamento farmacológico , Hormônios Liberadores de Hormônios Hipofisários/sangue , Testosterona/sangueRESUMO
The nitrate and nitrite contents in cheese of the Gouda type, produced by the Milk Complex of Havanna, were investigated and compared with those found in 5 types of imported cheese consumed in Cuba. No significant differences were found in the mean value (mean) of NaNO3, whereas in the mean value (mean) of NaNO2 differences were found when comparing all cheeses against each other. From these results it is inferred that the consumption of Gouda type cheese does not represent an important contribution to the daily intake of nitrates and nitrites, precursors in the synthesis of cancerogenic N-nitroso compounds.