RESUMO
BACKGROUND: Skin cryobanks represent important tools for the conservation of the maximum genetic representation of a population, especially those with a certain degree of threat to extinction, such as the ocelot. A relevant step towards the proper establishment of these banks is the definition of adequate cryopreservation techniques for the conservation of the skin. OBJECTIVE: We evaluated the effects of two different techniques [direct vitrification in cryovials (DVC) and solid-surface vitrification (SSV)] for the preservation of ear skin derived from ocelot. MATERIALS & METHODS: For both techniques, we vitrified the ear skin using Dulbeccos modified Eagles medium with 3.0 M dimethyl sulfoxide, 0.25 M sucrose, and 10% fetal bovine serum. Non-cryopreserved tissues were used as control (control group). All tissues were analyzed for their morphometric characteristics by conventional histology and morphological / functional analysis by cell ability during the culture. RESULTS: While tissues cryopreserved by DVC showed similar values for dermis thickness and number of perinuclear halos to the control, tissues cryopreserved by SSV showed similarities to the control regarding the number of melanocytes, percentage of collagen fibers, and numbers of viable cells by apoptosis analysis. Additionally, none of the vitrification techniques affected stratum corneum thickness, number of keratinocytes, tissue proliferative activity, cell viability, or metabolism. CONCLUSION: Both vitrification techniques (DVC and SSV) can be used for the conservation of ocelot skin; however, SSV guarantees a higher cellular quality after in vitro tissue culture in most of the parameters evaluated, such as viability, metabolism, and apoptosis analysis. doi.org/10.54680/fr23110110412.
Assuntos
Criopreservação , Vitrificação , Criopreservação/métodos , Crioprotetores/farmacologia , Dimetil Sulfóxido , Sacarose/farmacologiaRESUMO
Hamster breeding is becoming increasingly popular, mainly because of the simplicity of breeding, low cost, and characteristics of these animals: they are small, quiet, and do not require a large space. Because of this ease of keeping, some people choose to keep more than one animal of the same species in a single enclosure. In some cases, this sociability can lead to fights, because hamsters are extremely territorial animals, which can cause significant injuries. Knowledge of the most efficient methods for wound healing is essential in medicine for wild and exotic animals, as it allows faster clinical treatment and thus, stress reduction. The present case report refers to the therapeutic treatment of an extensive skin lesion in a golden hamster (Mesocricetus auratus) using ozonized sunflower oil. The treatment, which lasted 23 days, allowed complete healing of the lesion.
A criação de hamsters tem se tornado cada vez mais popular, especialmente pela facilidade de criação, pelo baixo custo e pelas características desses animais: são pequenos, silenciosos, e não necessitam de um grande espaço. Por essa facilidade de manejo, algumas pessoas decidem manter mais de um animal da mesma espécie em um único recinto. Em alguns casos, esse convívio pode causar brigas, visto que hamsters são animais extremamente territorialistas, podendo resultar em injúrias cutâneas significativas. O conhecimento sobre os métodos mais eficientes para a cicatrização de lesões é fundamental na medicina de animais silvestres e exóticos, possibilitando um manejo clínico mais rápido, consequentemente, redução do estresse. O presente relato refere-se ao manejo terapêutico de uma extensa lesão cutânea em um hamster sírio (Mesocricetus auratus) com o uso do óleo de girassol ozonizado. O tratamento, que teve 23 dias de duração, possibilitou a cicatrização total da lesão.