RESUMO
This study aimed to evaluate in vitro performance of near-infrared laser transillumination (NIR-LTI) for detecting early occlusal caries in permanent teeth and compare it with quantitative light-induced fluorescence (QLF), DIAGNOdent Pen (DDPen), and conventional radiography (CR). Ninety-four occlusal surfaces presenting International Caries Detection and Assessment System (ICDAS) scores ranging from 0 to 3 were selected. For the NIR-LTI examination, images were captured using a prototype, which consists of a laser beam (808 nm) and an infrared CCD camera. One occlusal site on each tooth was assessed twice by two examiners. The teeth were prepared histologically and assessed for the presence of early caries. The intraexaminer correlation showed no difference between the NIR-LTI, DDPen, and QLF analytical methods, but all these methods differed from CR. Interexaminer reproducibility was moderate for NIR-LTI, which showed sensitivity (0.68), specificity (0.85), accuracy (0.73), and area under the receiver-operating characteristic (ROC) curve (0.76) similar to those of the fluorescence method and different from those of the CR. In conclusion, the performance of NIR-LTI was comparable to that of DDPen and QLF and may therefore be considered a valid and reliable alternative for the diagnosis of incipient lesions on the occlusal surface of permanent teeth.
Assuntos
Cárie Dentária/diagnóstico , Lasers Semicondutores , Dente Pré-Molar/patologia , Esmalte Dentário/patologia , Diagnóstico Precoce , Humanos , Dente Molar/patologia , Curva ROC , Reprodutibilidade dos Testes , TransiluminaçãoRESUMO
OBJECTIVES: The purpose of this randomized double-blinded clinical trial was to test the efficacy and tooth sensitivity promoted by the use of an in-office 15% H(2)O(2) bleaching agent containing nanoparticles of TiO_N photocatalyzed with LED/laser light (HP15) and a control of 35% H2O2 (HP35). METHODS: Forty healthy volunteers, both sexes, aged 18 to 25 yr, were randomly distributed in 2 groups: HP15 (n = 20) was treated in 3 sessions of 48 min each, and HP35 (n = 20) was treated in 3 sessions of 45 min each. The efficacy (E) was evaluated by ΔE values measured via reflectance spectroscopy. The tooth sensitivity (S) was analyzed by visual analog scale (low, average, high, very high). The absolute risk reduction and the number needed to treat index were calculated. The data were analyzed by mixed repeated measures analysis of variance with Bonferroni-correction t test (α = 0.05). RESULTS: For the efficacy, significant differences were found for number of bleaching sessions (p = .0001; [Formula: see text] = 0.73 and π = 1.000) and for the interaction of number of sessions and bleaching protocols (p = .0001; [Formula: see text] = 0.319 and π = 1.000. The tooth sensitivity level showed significant differences only between the bleaching protocols. Absolute risk reduction calculated was 52% and number needed to treat, 1.92. CONCLUSIONS: The bleaching agent with the lower concentration (HP15) promoted lower levels of tooth sensitivity and presented greater efficacy compared to the control (HP35) in patients between 18 and 25 yr old. The limitation of short-term evaluation did not provide information about the longevity of the tooth bleaching (Brazilian Clinical Trials Registry Re Bec no. U1111-1150-4466).
Assuntos
Peróxido de Hidrogênio/administração & dosagem , Nitrogênio/administração & dosagem , Fármacos Fotossensibilizantes/administração & dosagem , Titânio/administração & dosagem , Clareamento Dental/métodos , Adolescente , Adulto , Sensibilidade da Dentina/prevenção & controle , Método Duplo-Cego , Feminino , Humanos , Análise de Intenção de Tratamento , Lasers Semicondutores/uso terapêutico , Masculino , Nanopartículas/química , Medição da Dor/métodos , Espectrofotometria/métodos , Fatores de Tempo , Clareadores Dentários/administração & dosagem , Resultado do Tratamento , Adulto JovemRESUMO
Between the period of January 1994 and June 1995, the results of Autotransfusion Program of the Hospital do Aparelho Motor SARAH-BSB were evaluated. The goals of the program are: 1. Minimise and, if possible, eliminate the risks of homologous transfusion; 2. Increase the security, efficiency and cost to benefits relation of the hemotherapic procedures; 3. Minimise the demand of blood products for those patients with difficult compatible blood to be found. The program has received 194 patients, among these, 100 have been selected for this study because they had had blood collection and surgery concluded. The average age of the patients was 34.3 years (11/72 years) median was 30 years and mode 15 years. The patients have undergone elective orthopaedic operations. Autologous transfusions have been made by predeposit and intraoperative salvage (Cell Saver). Six patients (6%) presented mild reactions associated to blood drawn. Additional homologous blood was needed to 20 (20%) patients. Our experience demonstrates the security and efficiency of transfusion in patients from childhood until elderly. It recommends the creation and expansion of existing programs using autologous transfusion in services with elective surgery. We also consider that nursing care stimulates patients participation in the program.
Assuntos
Transfusão de Sangue Autóloga , Procedimentos Cirúrgicos Eletivos , Cuidados Intraoperatórios/métodos , Cuidados Pré-Operatórios/métodos , Adolescente , Adulto , Coleta de Amostras Sanguíneas , Humanos , Cooperação do Paciente , Avaliação de Programas e Projetos de SaúdeRESUMO
The time course of prolyl endopeptidase (PE) activity, measured using 7-(N-succinyl-Gly-Pro)-4-methyl-coumarinamide as substrate, was determined in the developing chick retina, and in monolayer and aggregate culture of embryonic retinal cells. PE activity/retina increased 12.5-fold between embryonic days 7 and 12 and remained constant from the 12th embryonic day until the 3rd post-hatched day. PE activity/retina decreased 2.3-fold from the 3rd to the 9th post-hatched day. The levels of PE specific activity in aggregates and in retina were similar, whereas they were 44-81% higher in monolayer than in aggregate cultures between 3 and 13 days in culture. The data suggest that the development of PE activity and of plexiform layers occurs in parallel during chick retina ontogenesis, and that the chick retina can be an adequate in vivo and in vitro model to study PE development.