RESUMO
The microbiotherid marsupial Dromiciops gliroides inhabits the temperate forests of the Southern hemisphere, facing seasonal nutritional and energetic bottlenecks due to its apparently facultative insectivory/frugivory. In order to understand the physiological processes behind this ecological pattern, we studied the morpho-physiological changes that D. gliroides exhibits after dietary acclimation, in a sample of 21 wild-caught individuals fed over 1 month with ad libitum diet of: (1) fruit, (2) insects or (3) a mix of insects and fruit. In addition, we measured oxygen consumption (VO(2)) at resting conditions. We also performed enzyme assays (sucrase, maltase, trehalase and aminopeptidase N) and measurements of organ morphology. We found that D. gliroides cannot fulfil its nutrient requirements only from insects or fruit. It needs a mixed diet in order to maintain its body mass and energy balance. However, as a response of diet acclimation, individuals showed several-fold changes in the activities of aminopeptidase-N, maltase and sucrase (but not trehalase). This result, both the magnitude of change and the simultaneous effects on three enzymes suggests that D. gliroides could exhibit adaptive phenotypic plasticity in the activity of intestinal enzymes. This study suggests also that D. gliroides, the only living representative of the Microbiotheria order, exhibits physiological adaptations to a generalist diet.
Assuntos
Metabolismo Energético , Intestino Delgado/enzimologia , Marsupiais/fisiologia , Adaptação Fisiológica , Animais , Chile , Dieta , Intestino Delgado/anatomia & histologia , Fígado/anatomia & histologia , Marsupiais/metabolismo , Tamanho do Órgão , Consumo de Oxigênio , Fenótipo , Redução de PesoRESUMO
In evolutionary physiology, studies of inter-individual variation (i.e. repeatability) in functional capacities are valuable as they indicate - within populations - what attributes could respond to natural selection. Although repeatability and quantitative genetics of physiological traits in energy metabolism of eutherian mammals have been well characterized, few or no studies have been performed on marsupials. We studied the repeatability (i.e. intraclass correlation coefficient, tau) of bioenergetics for Monito del Monte (Dromiciops gliroides), the sole living representative of an otherwise extinct marsupial order (Microbiotheria). We measured resting metabolic rate as CO(2) production (V(CO(2))) and O(2) consumption (V(O(2))) simultaneously, together with minimum thermal conductance (C), evaporative water loss (EWL) and respiratory quotient (RQ), in a sample of ca. 20 individuals. Our results suggest that D. gliroides exhibits poor control of body temperature (T(b)), with a thermal amplitude of ca. 10 degrees C in normothermia. As a consequence, repeatability of T(b) and metabolic rate (either as V(CO(2)) or V(O(2))) were relatively low (tau(T)(b)=0.25+/-0.04, tau(VCO2)=0.14+/-0.03, tau(V)(O2)=0.24+/-0.02, jackknife estimations of standard errors). Thermal conductance exhibited near-zero or negative repeatability and was lower than expected for marsupials. However, we found significant repeatability for RQ and EWL (tau=0.32+/-0.03 and 0.49+/-0.09, respectively). In general, these results suggest that Monito del Monte exhibits some ;reptilian' physiological characteristics. The relatively low repeatability of physiological variables, which otherwise exhibit large inter-individual and genetic variance in eutherian mammals, suggests that these capacities do not exhibit evolutionary potential in the ancient order Microbiotheria.