RESUMO

The strain G89HT of Clostridium argentinense obtained by culture selection of the prototype G89 strain producing high titers of type G botulinal toxin was studied. Its cultural, biochemical and toxigenic characteristics and the presence of plasmids were tested. Both strains showed similar physiological features and carried a 83 MDa plasmid. A 170 MDa plasmid was also recognized in the G89HT strain. Notably, this strain was better sporulating and showed a higher toxigenicity than the prototype G89 C. argentinense strain. These two characteristics might permit a long term storage and perhaps yield high antitoxin titres.

Assuntos

Toxinas Botulínicas/metabolismo , Clostridium/metabolismo , Proteínas de Bactérias/análise , Clostridium/crescimento & desenvolvimento , Clostridium/patogenicidade , Meios de Cultura/metabolismo , Testes de Neutralização , Plasmídeos/genética , Plasmídeos/isolamento & purificação , Esporos Bacterianos/genética , Esporos Bacterianos/crescimento & desenvolvimento , Esporos Bacterianos/metabolismo , Tripsina/metabolismo , Virulência/genética