RESUMO
Central giant cell lesion is an uncommon benign jaw lesion, with uncertain aetiology, and variable clinical behaviour. Studies of molecular markers may help to understand the nature and behaviour of this lesion, and eventually may represent a target for pharmacological approaches to treatment. The aim of this study was to analyse the expression of glucocorticoid and calcitonin receptors in central giant cell lesions before and after treatment with intralesional steroid. Paraffin-embedded blocks from patients who underwent treatment with intralesional triamcinolone hexacetonide injections were stained immunohistochemically. Biological material from patients who underwent a surgical procedure after treatment were tested immunohistochemically. 18 cases (9 aggressive and 9 non-aggressive) were included. The difference in calcitonin receptor expression was not statistically significant between the aggressive and non-aggressive lesions and between the patients with a good response and those with a moderate/negative response to treatment. Glucocorticoid receptor expression in the multinucleated giant cells was higher in patients with a good response. It can be postulated that immunohistochemical staining for glucocorticoid receptors may provide a tool for selecting the therapeutic strategy. An H-score greater than 48 for glucocorticoid receptors in multinucleated giant cells predicted a good response in this study.
Assuntos
Granuloma de Células Gigantes/patologia , Doenças Maxilomandibulares/patologia , Receptores da Calcitonina/análise , Receptores de Glucocorticoides/análise , Adolescente , Adulto , Criança , Pré-Escolar , Feminino , Células Gigantes/patologia , Glucocorticoides/administração & dosagem , Glucocorticoides/uso terapêutico , Granuloma de Células Gigantes/tratamento farmacológico , Granuloma de Células Gigantes/cirurgia , Humanos , Injeções Intralesionais , Doenças Maxilomandibulares/tratamento farmacológico , Doenças Maxilomandibulares/cirurgia , Masculino , Doenças Mandibulares/tratamento farmacológico , Doenças Mandibulares/patologia , Doenças Mandibulares/cirurgia , Doenças Maxilares/tratamento farmacológico , Doenças Maxilares/patologia , Doenças Maxilares/cirurgia , Células Estromais/patologia , Resultado do Tratamento , Triancinolona Acetonida/administração & dosagem , Triancinolona Acetonida/uso terapêutico , Adulto JovemRESUMO
AIMS: This study investigated the possible correlation between the phenotypical and genotypical characteristics of Microsporum canis isolated from cats and dogs in north-east Brazil. METHODS AND RESULTS: The mycological study was conducted by direct microscopic examination and by fungal culture. Polymerase chain reaction-restriction enzyme analysis and random amplification of polymorphic DNA techniques were used for the genotypical analysis. The morphological analysis showed a considerable diversity of colonies as well as different morphologies of conidia, despite the M. canis strains having been isolated under the same conditions. However, the molecular analysis showed that all analysed strains are genetically similar. CONCLUSIONS: This study, based on phenotypical and molecular analysis, evidences the wide spectrum of phenotypical variations in M. canis in contrast to the stable genotypes of such dermatophytes. SIGNIFICANCE AND IMPACT OF THE STUDY: The findings of this study indicate that M. canis isolated from cats and dogs with dermatophytosis in north-east Brazil may be clones, well adapted to the conditions of this region, despite M. canis showing different morphological features.
Assuntos
Doenças do Gato/microbiologia , Dermatomicoses/veterinária , Doenças do Cão/microbiologia , Microsporum/genética , Animais , Brasil/epidemiologia , Doenças do Gato/epidemiologia , Gatos , Meios de Cultura , DNA Fúngico/genética , Dermatomicoses/epidemiologia , Dermatomicoses/microbiologia , Doenças do Cão/epidemiologia , Cães , Genótipo , Técnicas de Amplificação de Ácido Nucleico/métodos , Fenótipo , Reação em Cadeia da Polimerase/métodos , Polimorfismo Genético , Mapeamento por Restrição/métodosRESUMO
Inadequate doses or prolonged chemotherapy can be cytotoxic or genotoxic to cancer patients, increasing the risk for the development of a second cancer, particularly acute leukemia. The association between therapeutic and genotoxic properties of oncocalyxone A (Onco A), make cytotoxic tests (mitotic index and chromosomal aberrations) fundamental in the accompaniment of the effects of this active compound. Therefore, the aim of the present study was to determine the genotoxic action of Onco A in vitro, during different phases of the cell cycle, utilizing primary cultures of lymphocytes of healthy individuals. The results showed that Onco A is cytotoxic during the cell cycle phases G1, G1/S, and S, however, not in G2. Onco A did not demonstrate a genotoxic effect in any of the cell cycle phases at the concentration studied. It is concluded that during the period of exposure, this active substance inhibits DNA synthesis and consequently cell division. Therefore, the absence of such genotoxicity for Onco A in the tests performed in this study provides important information in regard to the therapeutic use of this agent. Further studies are necessary to better understand the molecular mechanism of action of Onco A.
Assuntos
Antraquinonas/toxicidade , Boraginaceae/química , Ativação Linfocitária/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Fito-Hemaglutininas/imunologia , Adolescente , Adulto , Antraquinonas/química , Células Cultivadas , Aberrações Cromossômicas/induzido quimicamente , Feminino , Humanos , Linfócitos/citologia , Masculino , Testes de Mutagenicidade , Extratos Vegetais/química , Extratos Vegetais/toxicidade , Plantas Medicinais/químicaRESUMO
Inadequate doses or prolonged chemotherapy can be cytotoxic or genotoxic to cancer patients, increasing the risk for the development of a second cancer, particularly acute leukemia. The association between therapeutic and genotoxic properties of oncocalyxone A (Onco A), make cytotoxic tests (mitotic index and chromosomal aberrations) fundamental in the accompaniment of the effects of this active compound. Therefore, the aim of the present study was to determine the genotoxic action of Onco A in vitro, during different phases of the cell cycle, utilizing primary cultures of lymphocytes of healthy individuals. The resultsshowed that Onco A is cytotoxic during the cell cycle phases G1, G1/S, and S,however, not in G2. Onco A did not demonstrate a genotoxic effect in any of the cell cycle phases at the concentration studied. It is concluded that during the period of exposure, this active substance inhibits DNA synthesis and consequently cell division. Therefore, the absence of such genotoxicity for Onco A in the tests performed in this study provides important information in regard tothe therapeutic use of this agent. Further studies are necessary to betterunderstand the molecular mechanism of action of Onco A.