RESUMO
Sleep disorders are increasingly being characterized in modern society as contributing to a host of serious medical problems, including obesity and metabolic syndrome. Changes to the microbial community in the human gut have been reportedly associated with many of these cardiometabolic outcomes. In this study, we investigated the impact of sleep length on the gut microbiota in a large cohort of 655 participants of African descent, aged 25-45, from Ghana, South Africa (SA), Jamaica, and the United States (US). The sleep duration was self-reported via a questionnaire. Participants were classified into 3 sleep groups: short (<7hrs), normal (7-<9hrs), and long (≥9hrs). Forty-seven percent of US participants were classified as short sleepers and 88% of SA participants as long sleepers. Gut microbial composition analysis (16S rRNA gene sequencing) revealed that bacterial alpha diversity negatively correlated with sleep length (p<0.05). Furthermore, sleep length significantly contributed to the inter-individual beta diversity dissimilarity in gut microbial composition (p<0.01). Participants with both short and long-sleep durations exhibited significantly higher abundances of several taxonomic features, compared to normal sleep duration participants. The predicted relative proportion of two genes involved in the butyrate synthesis via lysine pathway were enriched in short sleep duration participants. Finally, co-occurrence relationships revealed by network analysis showed unique interactions among the short, normal and long duration sleepers. These results suggest that sleep length in humans may alter gut microbiota by driving population shifts of the whole microbiota and also specific changes in Exact Sequence Variants abundance, which may have implications for chronic inflammation associated diseases. The current findings suggest a possible relationship between disrupted sleep patterns and the composition of the gut microbiota. Prospective investigations in larger and more prolonged sleep researches and causally experimental studies are needed to confirm these findings, investigate the underlying mechanism and determine whether improving microbial homeostasis may buffer against sleep-related health decline in humans.
Assuntos
Bactérias/classificação , Microbioma Gastrointestinal/fisiologia , Transtornos do Sono-Vigília/microbiologia , Sono/fisiologia , Adulto , Bactérias/genética , Bactérias/isolamento & purificação , Estudos de Coortes , Fezes/microbiologia , Feminino , Gana , Humanos , Jamaica , Masculino , Pessoa de Meia-Idade , RNA Ribossômico 16S/genética , Análise de Sequência de DNA/métodos , África do Sul , Inquéritos e Questionários , Estados UnidosRESUMO
OBJECTIVES: To investigate associations between self-reported sleep duration and cardiometabolic (CM) risk factors in African-origin adults residing in five countries spanning the epidemiologic transition. DESIGN: Cross-sectional. SETTING AND PARTICIPANTS: Ghanaian (nâ¯=â¯491), South African (nâ¯=â¯503), Jamaican (nâ¯=â¯508), Seychellois (nâ¯=â¯501) and American (nâ¯=â¯480) men and women. MEASUREMENTS: Self-reported sleep duration was obtained using questionnaires. Sex- and site-stratified logistic regression analyses investigated relationships between sleep duration, individual CM risk factors and a binary CM risk variable (presence of ≥3 CM risk factors), adjusting for age, physical activity and education. RESULTS: Sleep duration distributions varied by cohort: 44.5%, 41.4%, 35.9%, 16.8% and 2.5% of American, Jamaican, Seychellois, Ghanaian and South African men reported <7â¯h sleep per night respectively (pâ¯<â¯0.001). Similarly, 42.6%, 28.6%, 25.2%, 12.8% and 1.5% of American, Jamaican, Seychellois, Ghanaian and South African women reported <7â¯h sleep respectively (pâ¯<â¯0.001). American men reporting ≤6â¯h sleep were more likely to be in the elevated CM risk group (OR: 2.52, 95%CI: 1.02, 6.22, pâ¯=â¯0.045) and to have a high waist circumference (OR: 2.44, 95%CI: 1.07, 5.57, pâ¯=â¯0.034) compared to those reporting 8â¯h sleep. Jamaican women reporting ≤6â¯h sleep (OR: 2.53, 95%CI: 1.19, 5.36, pâ¯=â¯0.016) and American women reporting 7â¯h sleep (OR: 2.71, 95%CI: 1.17, 6.26, pâ¯=â¯0.002) were more likely to be obese than those reporting 8â¯h sleep. CONCLUSIONS: Associations between short sleep and CM risk factors were only evident in the American men and women and Jamaican women. Future interventions to address CM risk and sleep health may need to be country-specific when targeting high-risk populations.