RESUMO
Seroprevalence of HHV-8 has been studied in Malaysia, India, Sri Lanka, Thailand, Trinidad, Jamaica and the USA, in both healthy individuals and those infected with HIV. Seroprevalence was found to be low in these countries. In contrast, the African countries of Ghana, Uganda and Zambia showed high seroprevalences in both healthy and HIV-infected populations. This suggests that human herpes virus-8 (HHV-8) may be either a recently introduced virus or one that has extremely low infectivity. Nasopharyngeal and oral carcinoma patients from Malaysia, Hong Kong and Sri Lanka who have very high EBV titres to show that only 3/82 (3.7 percent) have antibody to HHV-8, demonstrating that there is little, if any, cross-relativity between antibodies to these two gamma viruses. (AU)
Assuntos
Adulto , Idoso , Humanos , Masculino , Feminino , Adolescente , Estudo Comparativo , Criança , Pessoa de Meia-Idade , Infecções por Herpesviridae/epidemiologia , Herpesvirus Humano 8/imunologia , Sarcoma de Kaposi/epidemiologia , África/epidemiologia , Idoso de 80 Anos ou mais , Linfoma de Burkitt/epidemiologia , Região do Caribe/epidemiologia , Neoplasias Nasofaríngeas/epidemiologia , Estudos Soroepidemiológicos , Estados Unidos/epidemiologiaRESUMO
Ten out of 26 leukaemic patients who had emigrated from the Caribbean region to the United Kingdom had adult T-cell leukaemia with associated serum antibodies to HTLV I. Antibodies to HTLV were also detected in sera from a small proportion of non-leukaemic Caribbean immigrants but not in any sera from other (non-ATL) T-cell leukaemias or a variety of control groups. The long period between immigration to the UK and diagnosis of leukaemia (up to 30 years) suggests that an extensive latent period in disease development may exist. Cell lines were isolated from two patients with HTLV antibody-positive ATL and were shown to be virus-positive by electron microscopy and immunofluorescence using antibodies to the p19 and p24 viral proteins. HTLV1 provirus integration and active transcription were demonstrated by Southern blotting of DNA and in situ hybridization respectively using molecularly cloned HTLV1 probes. Virus from one of these cell lines could be transmitted to normal T cells by co-cultivation.
Assuntos
Anticorpos Antineoplásicos/análise , Anticorpos Antivirais/análise , Deltaretrovirus/imunologia , Leucemia/imunologia , Infecções por Retroviridae/imunologia , Adolescente , Adulto , Anticorpos Monoclonais , População Negra , Medula Óssea/microbiologia , Medula Óssea/ultraestrutura , Linhagem Celular , Criança , DNA Viral/análise , Deltaretrovirus/análise , Feminino , Imunofluorescência , Humanos , Leucemia/microbiologia , Leucemia/patologia , Linfoma/imunologia , Linfoma/microbiologia , Linfoma/patologia , Masculino , Pessoa de Meia-Idade , Hibridização de Ácido Nucleico , Infecções por Retroviridae/microbiologia , Infecções por Retroviridae/patologia , Reino Unido , Índias Ocidentais/etnologiaRESUMO
Human T (thymus-derived)-cell leukemia/lymphoma virus (HTLV) is a new retrovirus first isolated from T-cell lines from a patient with cutaneous T-cell lymphoma from the southeastern United States. Closely related viruses have since been isolated from several patients with adult T-cell leukemia and lymphoma (and some normal persons) from different areas of the world. HTLV is not a genetically transmitted endogenous virus of humans, but it rather is acquired by postzygotic infection. Natural antibodies to several purified viral proteins have been observed in infected individuals. HTLV is transmissible in vitro to human cord blood T cells, and infection results in an increased growth rate, a reduced requirement for (and often independence from) T-cell growth factor, and an abrogation of the crisis period that usually occurs a month after the establishment of normal T-cell cultures. These data suggest that HTLV is the etiologic agent in some human cases of leukemia and lymphoma.