RESUMO
Objetivo: Caracterizar os aspectos trabalhistas das enfermeiras da Atenção Primária à Saúde nos municípios de tipologia urbana do estado da Bahia. Métodos: Estudo descritivo através de abordagem quantitativa a partir de informações obtidas com 32 enfermeiras, no período entre novembro de 2020 a maio de 2021, nos três maiores municípios do estado da Bahia. Resultados: Enfermeiras em sua maioria possuem vínculo trabalhista do tipo estatutário, com carga horária de 40 horas, atua há mais de um ano em unidades de saúde de modelo da Estratégia Saúde da Família, com diferenças salariais entre os municípios. Conclusão: Identificar aspectos trabalhistas das enfermeiras é de suma relevância, pois trazem implicações para melhoria das políticas de pessoal na Atenção Primária à Saúde. (AU)
Objective: To characterize the labor aspects of Primary Health Care nurses in urban municipalities of the state of Bahia. Methods: A descriptive study through a quantitative approach based on information obtained from 32 nurses, in the period between November 2020 and May 2021, in the 3 largest municipalities of the state of Bahia. Results: Most of the nurses have a statutory employment contract, work 40-hour shifts, and have been working for more than 1 year in health care units that follow the Family Health Strategy model, with wage differences among the municipalities. Conclusion: Identifying labor aspects of nurses is of utmost relevance, since they bring implications for improving personnel policies in Primary Health Care. (AU)
Objetivo: Caracterizar los aspectos laborales de las enfermeras de Atención Primaria de Salud en los municipios urbanos del estado de Bahia. Métodos: Estudio descriptivo a través de un enfoque cuantitativo basado en la información obtenida de 32 enfermeras, en el período comprendido entre noviembre de 2020 y mayo de 2021, en los tres mayores municipios del estado de Bahia. Resultados: La mayoría de las enfermeras tienen un contrato de trabajo legal, trabajan 40 horas y llevan más de 1 año en unidades sanitarias del modelo de la Estrategia de Salud Familiar, con diferencias salariales entre municipios. Conclusión: La identificación de los aspectos laborales de las enfermeras es de máxima relevancia, ya que aportan implicaciones para la mejora de las políticas de personal en Atención Primaria. (AU)
Assuntos
Atenção Primária à Saúde , Condições de Trabalho , Enfermeiras e EnfermeirosRESUMO
Objetivo:Investigar o conhecimento de estudantes de Enfermagem sobre estomias intestinais de eliminação. Método: Estudo descritivo, exploratório, analítico, com abordagem quantitativa, realizado em instituição de ensino superior pública do Piauí, com 115 estudantes de Enfermagem entre o oitavo e o 10º período do curso. A coleta foi realizada por meio de questionário online composto de dois instrumentos, um sobre o perfil sociodemográfico e escolar dos estudantes, e o outro, acerca do conhecimento sobre estomias intestinais de eliminação. A análise dos dados deu-se por meio de análises descritivas (frequências absolutas e relativas, médias e desvio padrão) e inferenciais, a fim de verificar associação entre as variáveis, com os testes χ2 e exato de Fisher. Resultados: A maioria dos estudantes de Enfermagem possui déficit quanto aos aspectos relacionados às assistências pré-operatória e pós-operatória imediata e mediata. Observou-se diferença significativamente estatística na variável índice de rendimento acadêmico, e viu-se que os estudantes com rendimento acadêmico superior a 9 obtiveram maior número de acertos em comparação aos demais. Conclusão: Existem lacunas de conhecimento entre os estudantes de Enfermagem, sobretudo na assistência de enfermagem nos cuidados pré-operatório e no pós-operatório imediato e mediato, o que por sua vez pode comprometer a qualidade da assistência prestada.
Objective:To investigate the knowledge of nursing students about intestinal elimination stomas. Method: Descriptive, exploratory, analytical study, with a quantitative approach, carried out in a public higher education institution in Piauí, Brazil, with 115 nursing students between the eighth and the tenth period of the course. The data collection was carried out through an online questionnaire composed of two instruments, one about the sociodemographic and school profile of the students, and the other about knowledge on intestinal ostomies for elimination. Data analysis was carried out through descriptive analysis (absolute and relative frequencies, means and standard deviation) and inferential analysis, in order to verify association between variables, with χ2and Fisher's exact tests. Results: Most nursing students have a deficit regarding aspects related to preoperative and immediate and mediate postoperative care. There was a statistically significant difference in the academic performance index variable, and it was observed that students with academic performance greater than 9 obtained a higher number of correct answers compared to the others. Conclusion: There are knowledge gaps among nursing students, especially in nursing care in preoperative care, and in the immediate and mediate postoperative period, which in turn can compromise the quality of care provided.
Objetivo:Investigar el conocimiento de estudiantes de enfermería sobre estomas de eliminación intestinal. Método: Estudio descriptivo-exploratorio, analítico, con abordaje cuantitativo, realizado en una Institución de Enseñanza Superior (IES) pública de Piauí, con 115 estudiantes de enfermería, entre el octavo y décimo período del curso. La recolección se realizó a través de un cuestionario en línea compuesto por dos instrumentos, uno sobre el perfil sociodemográfico y escolar de los estudiantes, y otro, un instrumento validado sobre conocimientos sobre ostomías intestinales para eliminación. El análisis de los datos se realizó mediante análisis descriptivo (frecuencias absolutas y relativas, medias y desviación estándar) e inferencial, con el fin de verificar la asociación entre las variables, con las pruebas Chi-cuadrado y exacta de Fisher. Resultados: La mayoría de los estudiantes de enfermería presentan un déficit en los aspectos relacionados con los cuidados preoperatorios y postoperatorios inmediatos y medios. Hubo diferencia estadísticamente significativa en la variable índice de rendimiento académico, y se observó que los estudiantes con rendimiento académico superior a 9,0 obtuvieron mayor número de respuestas correctas en comparación con los demás. Conclusión: Existen lagunas de conocimiento entre los estudiantes de enfermería, especialmente en el cuidado de enfermería en el preoperatorio, y en el postoperatorio inmediato y mediato, lo que a su vez puede comprometer la calidad de la atención prestada.
Assuntos
Estudantes de Enfermagem , Estomia , Conhecimento , Cuidados de EnfermagemRESUMO
Aviculture is a developed and important industry worldwide. However, it is an industry that produces solid waste such as bedding. As an attempt to reduce environmental impact and productive activity costs, beds are reused by several consecutive lots which can increase microorganism concentration and lead to unsanitary conditions. In this regard, it is essential to adopt a litter pre-treatment during the gap sanitary period between lots to avoid passing problems to the birds from the current flock to the subsequent flock and to guarantee the litter quality. Several factors must be considered to guarantee that there is minimal damage to chicken production. Therefore, this literature review aims to approach the main factors that affect the thermal comfort and chicken litter quality, as well as alternatives used as an alternative biological treatment to guarantee its reuse quality.
Assuntos
Galinhas , Aves Domésticas , AnimaisRESUMO
Hypsarrhythmia is a specific chaotic morphology, present in the interictal period of the electroencephalogram (EEG) signal in patients with West Syndrome (WS), a severe form of childhood epilepsy and that, recently, was also identified in the examinations of patients with Zika Virus Congenital Syndrome (ZVCS). This innovative work proposes the development of a computational methodology for analysis and differentiation, based on the time-frequency domain, between the chaotic pattern of WS and ZVCS hypsarrhythmia. The EEG signal time-frequency analysis is carried out from the Continuous Wavelet Transform (CWT). Four joint moments-joint mean-[Formula: see text], joint variance-[Formula: see text], joint skewness-[Formula: see text], and joint kurtosis-[Formula: see text]-and four entropy measurements-Shannon, Log Energy, Norm, and Sure-are obtained from the CWT to compose the representative feature vector of the EEG hypsarrhythmic signals under analysis. The performance of eight classical types of machine learning algorithms are verified in classification using the k-fold cross validation and leave-one-patient-out cross validation methods. Discrimination results provided 78.08% accuracy, 85.55% sensitivity, 73.21% specificity, and AUC = 0.89 for the ANN classifier.
Assuntos
Espasmos Infantis , Infecção por Zika virus , Zika virus , Algoritmos , Eletroencefalografia/métodos , Entropia , Humanos , Processamento de Sinais Assistido por Computador , Máquina de Vetores de Suporte , Síndrome , Análise de Ondaletas , Infecção por Zika virus/complicaçõesRESUMO
The use of vibrational spectroscopy, such as near infrared (NIR) and Raman, combined with multivariate analysis methods to analyze agricultural products are promising for investigating genetically modified organisms (GMO). In Brazil, cotton is grown under humid tropical conditions and is highly affected by pests and diseases, requiring the use of large amounts of phytosanitary chemicals. To avoid the use of those pesticides, genetic improvement can be carried out to produce species tolerant to herbicides, resistant to fungi and insects, or even to provide greater productivity and better quality. Even with these advantages, it is necessary to manage and limit the contact of transgenic species with native ones, avoiding possible contamination or even extinction of conventional species. The identification of the presence of GMOs is based on complex DNA-based analysis, which is usually laborious, expensive, time-consuming, destructive, and generally unavailable. In the present study, a new methodology to identify GMOs using partial least squares discriminant analysis (PLS-DA) on NIR and Raman data is proposed to distinguish conventional and transgenic cotton seed genotypes, providing classification errors for prediction set of 2.23% for NIR and 0.0% for Raman.
Assuntos
Óleo de Sementes de Algodão , Espectroscopia de Luz Próxima ao Infravermelho , Análise Discriminante , Genótipo , Análise dos Mínimos Quadrados , Sementes/genéticaRESUMO
Punicalagin, the principal ellagitannin of Lafoensia pacari leaves, has proven antioxidant activity, and standardized extracts of L. pacari can be topically used for skin aging management. We hypothesized that Pluronic nanomicelles or vesicles could solubilize sufficiently large amounts of the standardized extracts of L. pacari and provide chemical stability to punicalagin. The standardized extracts of L. pacari were obtained with an optimized extraction procedure, and the antioxidant activity was characterized. Formulations containing Pluronic at 25% and 35% were obtained with or without Span 80. They were characterized by average diameter, polydispersity index, punicalagin content, physicochemical stability, and rheology. A release and skin permeation study was carried out in vertical diffusion cells. The extraction procedure allowed quantifying high punicalagin content (i.e., 141.61 ± 3.87 mg/g). The standardized extracts of L. pacari showed antioxidant activity for all evaluated methods. Pluronic at 25 and Pluronic at 35 with standardized extracts of L. pacari showed an average diameter of about 25 nm. The addition of Span 80 significantly increased the mean diameter by 15-fold (p < 0.05), indicating the spontaneous formation of vesicles. Pluronic formulations significantly protected punicalagin from chemical degradation (p < 0.05). Pluronic at 25 formulations presented as free-flowing liquid-like systems, while Pluronic at 35 resulted in an increase of about 44-fold in |Æ*|. The addition of Span 80 significantly reduced the Pluronic sol-gel transition temperature (p < 0.05), indicating the formation of vesicles. Formulations with Span 80 significantly enhanced punicalagin skin permeation compared to formulations without Span 80 (p < 0.05). Formulations with Span 80 were demonstrated to be the most promising formulations, as they allowed significant permeation of punicalagin (about 80 to 315 µg/cm2), which has been shown to have antioxidant activity.
Assuntos
Taninos Hidrolisáveis , Lythraceae , Antioxidantes/farmacologia , Lythraceae/química , Micelas , Extratos Vegetais/farmacologia , PoloxâmeroRESUMO
Transdermal administration of raloxifene hydrochloride (RLX)-loaded nanostructured lipid carriers (NLCs) has been proposed to circumvent its low oral bioavailability (2%). Preformulation studies were carried out to evaluate drug-excipient compatibility of various adjuvants commonly used for NLC preparation (waxes, cholesterol, compritol, gelucire, span 60, span 80, span 85, tween 80, poloxamer 188, oleic acid, caprylic/capric triglyceride, and castor oil). It was used differential scanning calorimetry (DSC), isothermal stress testing (IST), and solubility studies. The most promising excipients were chosen for NLC obtention, and full characterization was done, including in vitro skin permeation. DSC curves suggested drug-excipient interaction among some compounds, and the IST study showed incompatibility of RLX with waxes, compritol, cholesterol, span 60, and poloxamer 188. Solubility studies helped select gelucire, caprylic/capric triglyceride, span 80, and tween 80 for NLC production. Twelve NLCs were obtained (NLC1 to NLC12), but NLC7 and NLC8 were the most promising ones. In vitro release studies demonstrated that NLC7 and NLC8 were able to control RLX release (14.74 and 9.07% at 24 h, respectively) compared with the unloaded drug (> 90% at 24 h). Unloaded RLX did not permeate the diffusion cells' receptor medium and showed higher drug skin retention (11-fold) than RLX-loaded NLC. NLC reduced RLX skin retention, favoring drug permeation to deeper skin layers. NLC7 increased drug flux is 2.4-fold. NLC7 is a promising formulation for RLX transdermal drug delivery.
Assuntos
Nanopartículas , Nanoestruturas , Administração Cutânea , Portadores de Fármacos/química , Excipientes/química , Lipossomos , Nanopartículas/química , Tamanho da Partícula , Poloxâmero , Polissorbatos , Cloridrato de Raloxifeno , Triglicerídeos , CerasRESUMO
OBJETIVO: o objetivo deste estudo foi investigar possíveis associações entre Burnout, uso de hipnóticos e qualidade do sono em estudantes de medicina. MÉTODO: foi realizado um estudo transversal quantitativo com estudantes de medicina de um centro universitário do nordeste do Brasil, com metodologia de Aprendizagem Baseada em Problemas (ABP). O Maslach Burnout Inventory-Student Survey, o Pittsburgh Sleep Quality Index (PSQI) e um questionário sociodemográfico foram aplicados entre os alunos do pré-internato do curso. RESULTADOS: o estudo incluiu 523 alunos e encontrou 48 (9,2%) com critérios de diagnóstico tridimensional para Síndrome de Burnout (SB). A má qualidade do sono e o uso de hipnóticos para dormir foram associados à SB (p < 0,001 e p = 0,003, respectivamente). Não houve diferenças estatísticas quanto à idade, sexo, religião, estado civil, financiamento escolar e realização de trabalho remunerado, entre indivíduos com e sem SB. CONCLUSÃO: houve associação entre SB, uso de hipnóticos e má qualidade do sono em estudantes de medicina pré-internato.
OBJECTIVE: the aim of this study was to investigate possible associations between Burnout, use of hypnotics and sleep quality among medical students. METHOD: a cross-sectional quantitative study was conducted among medical students of a university center of northeastern Brazil, with the problem-based learning (PBL) methodology. The Maslach Burnout Inventory-Student Survey, the Pittsburgh Sleep Quality Index (PSQI) and a sociodemographic questionnaire were applied among pre-clerkship students of the course. RESULTS: the study included 523 students and found 48 (9.2%) with tridimensional diagnosis criteria for Burnout syndrome (BS). Poor sleep quality and use of hypnotic drugs for sleep were associated with BS (p < 0.001 and p = 0.003, respectively). There were no statistical differences in age, gender, religion, marital status, student financing and performing paid work, between individuals with and without BS. CONCLUSION: there was an association between BS, use of hypnotics and poor sleep quality among pre-clerkship medical students.
OBJETIVO: el objetivo de este estudio fue investigar las posibles asociaciones entre el síndrome de Burnout, el uso de hipnóticos y la calidad del sueño entre estudiantes de medicina. MÉTODOS: se realizó un estudio cuantitativo transversal entre estudiantes de medicina de un centro universitario del noreste de Brasil, con metodología de aprendizaje basado en problemas (ABP). Se aplicó el Maslach Burnout Inventory-Student Survey, el Pittsburgh Sleep Quality Index (PSQI) y un cuestionario sociodemográfico entre los estudiantes de pre-administrativo del curso. RESULTADOS: el estudio incluyó a 523 estudiantes y encontró 48 (9,2%) con criterios de diagnóstico tridimensional de síndrome de Burnout (SB). La mala calidad del sueño y el uso de fármacos hipnóticos para dormir se asociaron con SB (p <0,001 y p = 0,003, respectivamente). No hubo diferencias estadísticas en edad, sexo, religión, estado civil, financiamiento de los estudiantes y realización de trabajo remunerado, entre individuos con y sin SB. CONCLUSIÓN: hubo asociación entre SB, uso de hipnóticos y mala calidad del sueño entre los estudiantes de medicina antes de la rotación clínica.
Assuntos
Humanos , Masculino , Feminino , Educação Médica , Educação de Graduação em Medicina , Medicamentos Indutores do Sono , Esgotamento Psicológico , Distúrbios do Início e da Manutenção do SonoRESUMO
The present study aimed to investigate whether the serum biomarkers of immune response orchestrate the seroconversion status in patients with autoimmune diseases (AID) upon planned primary 17DD-YF vaccination. For this purpose a total of 161 individuals were enrolled in a prospective study, including patients with Rheumatoid Arthritis (RA = 38), Spondyloarthritis (SpA = 51), Systemic Lupus Erythematosus (SLE = 21) and Sjögren's Syndrome (SS = 30) along with a group of healthy controls (HC = 21). Analysis of plaque reduction neutralization test (PRNT) titers and seropositivity rates along with the 17DD-YF viremia and serum biomarkers were carried out at distinct time points (D0/D3-4/D5-6/D7/D14-28). The results demonstrated an overall lower PRNT titer and seropositivity rate (170 vs. 448; 77 vs. 95%) in AID as compared to HC, especially in SpA and SLE subgroups. No significant differences were observed in the viremia levels amongst groups. In general, a more prominent serum biomarker response was observed in AID as compared to HC, throughout the timeline kinetics. Remarkably, AID/PRNT(-) exhibited higher levels of several biomarkers at baseline as compared to AID/PRNT+. Moreover, while AID/PRNT(+) exhibited earlier increase in serum biomarkers at D3-4/D5-6, the AID/PRNT(-) displayed higher response at later time points (D7/D14-D28). Of note, a synchronic increase of IFN-γ at the peak of viremia (D5-6) was observed in HC and AID/PRNT(+) groups, whereas a later asynchronous IFN-γ response was reported for AID/PRNT(-) at D7. The biomarker profile tends to deflate at post-vaccination timeline, highlighting a putative immunomodulatory effect of live attenuated 17DD-YF vaccine in AID/PRNT(+), but not in AID/PRNT(-). Altogether these data suggested that inflammatory status prior vaccination, low IFN-γ at viremia peak and the occurrence of asynchronous biomarker storm after 17DD-YF vaccination may orchestrate the lack of neutralizing antibody response γ.
Assuntos
Doenças Autoimunes/imunologia , Vacina contra Febre Amarela/imunologia , Febre Amarela/prevenção & controle , Vírus da Febre Amarela/imunologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticorpos Neutralizantes/sangue , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/sangue , Anticorpos Antivirais/imunologia , Doenças Autoimunes/sangue , Estudos de Casos e Controles , Feminino , Voluntários Saudáveis , Humanos , Imunogenicidade da Vacina , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Soroconversão , Vacinação , Vacinas Atenuadas/administração & dosagem , Vacinas Atenuadas/imunologia , Febre Amarela/imunologia , Febre Amarela/virologia , Vacina contra Febre Amarela/administração & dosagem , Adulto JovemRESUMO
This work aimed to select an effective penetration enhancer (PE) for nail pretreatment, develop voriconazole (VOR)-loaded nanomicelles, and evaluate their ability to deliver VOR to the nail. A complete analysis of nail protein dynamics, bond rupture, and microstructure was performed. Alternative methods as electron paramagnetic resonance (EPR) and the Ellman's reagent (DTNB) assay were also evaluated. Nanomicelles were produced and characterized. The PE hydrated the hooves, following the order: urea ≈ cysteine ≈ glycolic acid < thioglycolic acid (TGA) < NaOH. SEM images and methylene blue assay showed enlarged pores and roughness of porcine hooves after incubation with NaOH and TGA. EPR was demonstrated to be the most sensitive technique. DTNB assay quantified higher thiol groups for samples treated with TGA (p < 0.05). A stratigraphic analysis with Raman spectroscopy demonstrated that hooves treated with TGA presented a higher SH/SS ratio at the edges, affecting protein secondary structure. In vitro permeation studies demonstrated significant VOR permeation (29.44 ± 6.13 µg/cm2), 10-fold higher than previous studies with lipid nanoparticles. After TGA pretreatment, VOR permeation was further enhanced (3-fold). TGA pretreatment followed by VOR-loaded nanomicelles demonstrates a promising approach for onychomycosis treatment. The novel methods for protein analysis were straightforward and helpful.