RESUMO

Salmonella Pullorum is a pathogen that infects several species of birds, mainly offspring, and among them, quails. Therefore, this study was performed in order to evaluate the S. Pullorum shedding in experimentally infected Japanese quails (Coturnix coturnix) and egg contamination ability. S. Pullorum ATCC lyophilized was inoculated in all birds (60 quails at 160 days). Before  the inoculation, rapid slide agglutination test was performed in all birds and individual cloacal  swabs were collected randomly. Birds from group A and group B received 0.5mL of inoculum containing 1.5x102 CFU and 7.5 x 108 CFU of S. Pullorum, respectively, directly in the crop. The cloacal swab samples were collected on 3, 6, 9, 16 and 23 days post-inoculation (dpi) and all eggs were collected. Positive cloacal swab samples were initiated at the 3rd dpi. A total of 30 pool of   eggs was analyzed, of which 73% positive for S. Pullorum until the 16th dpi. In conclusion, adult Japanese quails are possibly more resistant to the infection by S. Pullorum, but they can shed this pathogen in the environment as  well.

Salmonella Pulllorum é um patógeno que acomete várias espécies de aves, principalmente jovens, dentre essas, codornas. Dessa forma, o estudo teve como objetivo avaliar a eliminação da S.Pullorum em codornas japonesas (Coturnix coturnix) infectadas experimentalmente, bem como avaliar a capacidade de contaminação dos ovos. Foi utilizada uma cepa de S. Pullorum ATCC liofilizada para inoculação de todas as aves (60 codornas com 160 dias de idade). Antecedendo a inoculação, foi realizado exame de Soroaglutinação Rápida em Placas em todas as aves e coletados suabes cloacais individuais de aves escolhidas aleatoriamente. As aves do grupo A e do grupo B foram inoculadas, com 0,5 mL do inóculo contendo 1.5 x 102 UFC e 7.5 x 108 UFC, respectivamente, diretamente no papo. As coletas de suabes cloacais ocorreram nos dias 3, 6, 9, 16 e 23 pós-inoculação e todos os ovos foram coletados. Amostras positivas de suabes cloacais se iniciaram aos 03 dpi. Foi analisado um total de 30 pool de ovos, sendo 73% positivos para S.Pullorum até 16 dpi. Conclui-se dessa forma que possivelmente codornas japonesas adultas são mais resistentes a infecção por S.Pullorum, mas pode disseminar o agente ao meio  ambiente.

Assuntos

RESUMO

Introduction: Diabetes mellitus is the most common metabolic disease worldwide. Endothelial dysfunction characteristic of these patients is one of the major risk factors for atherosclerosis. Early diagnosis of endothelial dysfunction is essential for the treatment especially of non-invasive manner, such as flow mediated dilation. Physical exercise is capable of generating beneficial adaptations may improve endothelial function. Objective: Identify the effect of physical exercise, using the clinical technique of ultrasound in the assessment of the endothelial function of patients with metabolic syndrome or type 2 diabetes mellitus. Methods: Thirty-one patients with type 2 diabetes mellitus or metabolic syndrome were studied, with a mean age (± SD) of 58±6 years, randomized into three groups. The training was performed for 50 minutes, four times a week. Before and after six weeks of training, subjects performed the endurance test and a study of the endothelial function of the brachial artery by high-resolution ultrasound. Results: After hyperemia, the percentage of arterial diameter was significantly higher for the high-intensity group (HI before = 2.52±2.85mm and after = 31.81±12.21mm; LI before = 3.23±3.52mm and after = 20.61±7.76mm; controls before = 3.56±2.33mm and after = 2.43±2.14mm; p<0.05). Conclusions: The high-intensity aerobic training improved the vasodilatation response-dependent endothelium, recorded by ultrasound, in patients with metabolic syndrome and type 2 diabetes.

Introdução: Diabetes melittus é a doença metabólica mais comum no mundo. A disfunção endotelial característica desses pacientes é um dos principais fatores de risco para aterosclerose. O diagnóstico precoce da disfunção endotelial é imprescindível para o tratamento, sobretudo, de maneira não invasiva, tal como dilatação mediada pelo fluxo. O exercício físico é capaz de gerar adaptações benéficas podendo melhorar a função endotelial. Objetivo: Identificar o efeito do exercício físico de alta intensidade, usando a técnica clínica de ultrassom, na avaliação da função endotelial de pacientes com síndrome metabólica ou diabetes mellitus tipo dois. Métodos: Foram estudados 31 pacientes com diabetes mellitus tipo dois ou síndrome metabólica, com média de idade (± DP) de 58 ± 6 anos, distribuídos aleatoriamente em três grupos. O treinamento foi realizado por 50 minutos, quatro vezes por semana. Antes e depois de seis semanas de treinamento, os indivíduos realizaram o ensaio de resistência e um estudo da função endotelial por ultrassom de alta resolução da artéria braquial. Resultados: Depois da hiperemia, a porcentagem do diâmetro arterial foi significativamente maior para o grupo de alta intensidade. (AI antes = 2,52 ± 2,85 mm e depois = 31,81 ± 12,21 mm; BI antes = 3,23 ± 3,52 mm e depois = 20,61 ± 7,76 mm; controles antes = 3,56 ± 2,33 mm e depois = 2,43 ± 2,14 mm; p < 0,05). Conclusões: O treinamento aeróbico de alta intensidade melhorou o endotélio de modo dependente da resposta vasodilatadora, constatada por meio de ultrassom, em pacientes com síndrome metabólica e diabetes tipo 2.

Introducción: Diabetes mellitus es la enfermedad metabólica más común en todo el mundo. La disfunción endotelial característica de estos pacientes es uno de los principales factores de riesgo para la aterosclerosis. El diagnóstico precoz de la disfunción endotelial es esencial para el tratamiento, sobre todo de forma no invasiva como la dilatación mediada por flujo. El ejercicio es capaz de Generar adaptaciones beneficiosas mejorando la función endotelial. Objetivo: Identificar los efectos del ejercicio de alta intensidad, utilizando la técnica clínica del ultrasonido en la evaluación de la función endotelial en pacientes con síndrome metabólico o diabetes mellitus tipo 2. Métodos: Se estudiaron 31 pacientes con diabetes mellitus tipo 2 o síndrome metabólico, con una edad media (± DE) de 58 ± 6 años, divididos aleatoriamente en tres grupos. El entrenamiento se llevó a cabo durante 50 minutos, cuatro veces por semana. Antes y después de seis semanas de entrenamiento, los sujetos realizaron la prueba de resistencia y un estudio de la función endotelial mediante ultrasonido de alta resolución de la arteria braquial. Resultados: Después de hiperemia, el porcentaje de diámetro arterial fue significativamente mayor en el grupo de alta intensidad (AI antes = 2,52 ± 2,85 mm y después = 31,81 ± 12,21 mm; BI antes = 3,23 ± 3,52 mm y después = 20,61 ± 7,76 mm; controles antes = 3,56 ± 2,33 mm y después = 2,43 ± 2,14 mm; p < 0,05). Conclusiones: El entrenamiento aeróbico de alta intensidad, mejoró el endotelio de manera dependiente de la respuesta vasodilatadora, registrado por ultrasonido, en sujetos con síndrome metabólico y diabetes tipo 2.

RESUMO

O tifo aviário uma enfermidade de alto impacto no setor avícola, este trabalho tem como objetivo verificar os sinais clínicos da doença em codornas inoculadas experimentalmente com Salmonella Gallinarum. Foram utilizadas 54 codornas japonesas fêmeas distribuídas em dois grupos. O grupo inoculado (GI) com SG (32 codornas) e o grupo controle (GC) (16 aves). Seis aves foram eutansiadas antes de iniciar o experimento para coleta de órgãos (fígado, baço, folículos ovarianos, cecos e pulmão) e processamento microbiológico. As aves do GI receberam 0,7mL do inóculo (1.5x106 UFC/mL) e GC receberam e 0,7mL de solução fisiológica e foram observadas durante dez dias seguidos, em três períodos diários (8:00, 11:00 e 14:00) para observar os sinais clínicos da enfermidade. Após quatro dias de inoculação foi verificada prostração, apatia, penas eriçadas, diarreia, permanência da ave no canto da gaiola com os olhos fechados e relutância em movimentar-se (13/32). As alterações macroscópicas em aves que apresentaram sinais clínicos e foram eutanasiadas foram caracterizadas por esplenomegalia e hemorragia no baço em 84,6 % (11/13) e 23% (3/13), respectivamente, hepatomegalia e necrose no fígado em 15,4% (2/13) e 23,0% (3/13) respectivamente e hemorragia e atrofia em 15,4% (2/13) e 7,7% (1/13) dos folículos ovarianos. Nas aves que foram a óbito foi observado apenas hepatoesplenomegalia. Assim, podemos concluir que codornas inoculadas experimentalmente com Salmonella Gallinarum apresentam sinais clínicos típicos de febre tifoide observados em galinhas de exploração comercial.(AU)

Fowl typhoid a high-impact disease in the poultry industry, this study aims to verify the clinical signs of the disease in experimentally inoculated with quail Salmonella Gallinarum 54 Japanese female quails were distributed into two groups. The inoculated group (GI) SG (32 quail) and control group (CG) (16 birds). A total of six birds were euthanized before the start of the experiment, organs samples (liver, spleen, ovarian follicles, cecum and lung) were collected for the microbiological analysis. The birds of the GI group received 0,7mL of inoculum (1.5x106 CFU/mL) and birds of the CG group received 0,7 mL of saline solution. Thereafter, the quails were observed for ten consecutive days in three daily periods (8:00, 11:00 e 14:00) in order to observe clinical signs of disease. After four days of inoculation was verified prostration, apathy, ruffled feathers, diarrhea, bird's stay in the corner of the cage with closed eyes and reluctance to move (13/32). Some macroscopic changes were observed in euthanized birds with clinical signs like splenomegaly and splenic necrosis in 84,6% (11/13) and 23,0% (3/13) respectively, hepatomegaly and liver necrosis in 15,4% (2/13) e 23% (3/13) respectively, and atrophy and hemorrhage of ovarian follicles in 15,4% (2/13) and 7,7% (1/13) respectively. the birds that died was observed only hepatosplenomegaly. So we can conclude experimentally inoculated with Salmonella Gallinarum quail have the typical clinical signs of fowl typhoid observed in others birds of Galliforme order.(AU)

Assuntos

RESUMO

Abstract Salmonella Pullorum is a pathogen that infects several species of birds, mainly offspring, and among them, quails. Therefore, this study was performed in order to evaluate the S. Pullorum shedding in experimentally infected Japanese quails (Coturnix coturnix) and egg contamination ability. S. Pullorum ATCC lyophilized was inoculated in all birds (60 quails at 160 days). Before the inoculation, rapid slide agglutination test was performed in all birds and individual cloacal swabs were collected randomly. Birds from group A and group B received 0.5mL of inoculum containing 1.5x102 CFU and 7.5 x 108 CFU of S. Pullorum, respectively, directly in the crop. The cloacal swab samples were collected on 3, 6, 9, 16 and 23 days post-inoculation (dpi) and all eggs were collected. Positive cloacal swab samples were initiated at the 3rd dpi. A total of 30 pool of eggs was analyzed, of which 73% positive for S. Pullorum until the 16th dpi. In conclusion, adult Japanese quails are possibly more resistant to the infection by S. Pullorum, but they can shed this pathogen in the environment as well.

Resumo Salmonella Pulllorum é um patógeno que acomete várias espécies de aves, principalmente jovens, dentre essas, codornas. Dessa forma, o estudo teve como objetivo avaliar a eliminação da S.Pullorum em codornas japonesas (Coturnix coturnix) infectadas experimentalmente, bem como avaliar a capacidade de contaminação dos ovos. Foi utilizada uma cepa de S. Pullorum ATCC liofilizada para inoculação de todas as aves (60 codornas com 160 dias de idade). Antecedendo a inoculação, foi realizado exame de Soroaglutinação Rápida em Placas em todas as aves e coletados suabes cloacais individuais de aves escolhidas aleatoriamente. As aves do grupo A e do grupo B foram inoculadas, com 0,5 mL do inóculo contendo 1.5 x 102 UFC e 7.5 x 108 UFC, respectivamente, diretamente no papo. As coletas de suabes cloacais ocorreram nos dias 3, 6, 9, 16 e 23 pós-inoculação e todos os ovos foram coletados. Amostras positivas de suabes cloacais se iniciaram aos 03 dpi. Foi analisado um total de 30 pool de ovos, sendo 73% positivos para S.Pullorum até 16 dpi. Conclui-se dessa forma que possivelmente codornas japonesas adultas são mais resistentes a infecção por S.Pullorum, mas pode disseminar o agente ao meio ambiente.

RESUMO

O tifo aviário uma enfermidade de alto impacto no setor avícola, este trabalho tem como objetivo verificar os sinais clínicos da doença em codornas inoculadas experimentalmente com Salmonella Gallinarum. Foram utilizadas 54 codornas japonesas fêmeas distribuídas em dois grupos. O grupo inoculado (GI) com SG (32 codornas) e o grupo controle (GC) (16 aves). Seis aves foram eutansiadas antes de iniciar o experimento para coleta de órgãos (fígado, baço, folículos ovarianos, cecos e pulmão) e processamento microbiológico. As aves do GI receberam 0,7mL do inóculo (1.5x106 UFC/mL) e GC receberam e 0,7mL de solução fisiológica e foram observadas durante dez dias seguidos, em três períodos diários (8:00, 11:00 e 14:00) para observar os sinais clínicos da enfermidade. Após quatro dias de inoculação foi verificada prostração, apatia, penas eriçadas, diarreia, permanência da ave no canto da gaiola com os olhos fechados e relutância em movimentar-se (13/32). As alterações macroscópicas em aves que apresentaram sinais clínicos e foram eutanasiadas foram caracterizadas por esplenomegalia e hemorragia no baço em 84,6 % (11/13) e 23% (3/13), respectivamente, hepatomegalia e necrose no fígado em 15,4% (2/13) e 23,0% (3/13) respectivamente e hemorragia e atrofia em 15,4% (2/13) e 7,7% (1/13) dos folículos ovarianos. Nas aves que foram a óbito foi observado apenas hepatoesplenomegalia. Assim, podemos concluir que codornas inoculadas experimentalmente com Salmonella Gallinarum apresentam sinais clínicos típicos de febre tifoide observados em galinhas de exploração comercial.

Fowl typhoid a high-impact disease in the poultry industry, this study aims to verify the clinical signs of the disease in experimentally inoculated with quail Salmonella Gallinarum 54 Japanese female quails were distributed into two groups. The inoculated group (GI) SG (32 quail) and control group (CG) (16 birds). A total of six birds were euthanized before the start of the experiment, organs samples (liver, spleen, ovarian follicles, cecum and lung) were collected for the microbiological analysis. The birds of the GI group received 0,7mL of inoculum (1.5x106 CFU/mL) and birds of the CG group received 0,7 mL of saline solution. Thereafter, the quails were observed for ten consecutive days in three daily periods (8:00, 11:00 e 14:00) in order to observe clinical signs of disease. After four days of inoculation was verified prostration, apathy, ruffled feathers, diarrhea, bird's stay in the corner of the cage with closed eyes and reluctance to move (13/32). Some macroscopic changes were observed in euthanized birds with clinical signs like splenomegaly and splenic necrosis in 84,6% (11/13) and 23,0% (3/13) respectively, hepatomegaly and liver necrosis in 15,4% (2/13) e 23% (3/13) respectively, and atrophy and hemorrhage of ovarian follicles in 15,4% (2/13) and 7,7% (1/13) respectively. the birds that died was observed only hepatosplenomegaly. So we can conclude experimentally inoculated with Salmonella Gallinarum quail have the typical clinical signs of fowl typhoid observed in others birds of Galliforme order.

Assuntos

RESUMO

Salmonella Pullorum is a pathogen that infects several species of birds, mainly offspring, and among them, quails. Therefore, this study was performed in order to evaluate the S. Pullorum shedding in experimentally infected Japanese quails (Coturnix coturnix) and egg contamination ability. S. Pullorum ATCC lyophilized was inoculated in all birds (60 quails at 160 days). Before  the inoculation, rapid slide agglutination test was performed in all birds and individual cloacal  swabs were collected randomly. Birds from group A and group B received 0.5mL of inoculum containing 1.5x102 CFU and 7.5 x 108 CFU of S. Pullorum, respectively, directly in the crop. The cloacal swab samples were collected on 3, 6, 9, 16 and 23 days post-inoculation (dpi) and all eggs were collected. Positive cloacal swab samples were initiated at the 3rd dpi. A total of 30 pool of   eggs was analyzed, of which 73% positive for S. Pullorum until the 16th dpi. In conclusion, adult Japanese quails are possibly more resistant to the infection by S. Pullorum, but they can shed this pathogen in the environment as  well.(AU)

Salmonella Pulllorum é um patógeno que acomete várias espécies de aves, principalmente jovens, dentre essas, codornas. Dessa forma, o estudo teve como objetivo avaliar a eliminação da S.Pullorum em codornas japonesas (Coturnix coturnix) infectadas experimentalmente, bem como avaliar a capacidade de contaminação dos ovos. Foi utilizada uma cepa de S. Pullorum ATCC liofilizada para inoculação de todas as aves (60 codornas com 160 dias de idade). Antecedendo a inoculação, foi realizado exame de Soroaglutinação Rápida em Placas em todas as aves e coletados suabes cloacais individuais de aves escolhidas aleatoriamente. As aves do grupo A e do grupo B foram inoculadas, com 0,5 mL do inóculo contendo 1.5 x 102 UFC e 7.5 x 108 UFC, respectivamente, diretamente no papo. As coletas de suabes cloacais ocorreram nos dias 3, 6, 9, 16 e 23 pós-inoculação e todos os ovos foram coletados. Amostras positivas de suabes cloacais se iniciaram aos 03 dpi. Foi analisado um total de 30 pool de ovos, sendo 73% positivos para S.Pullorum até 16 dpi. Conclui-se dessa forma que possivelmente codornas japonesas adultas são mais resistentes a infecção por S.Pullorum, mas pode disseminar o agente ao meio  ambiente.(AU)

Assuntos

RESUMO

The high-intensity interval training (HIIT) is characterized by vigorous and intermittent performing certain exercise separated by periods of passive or active rest with low intensity. This training model can be as effective as continuous endurance training. However, the aim of this study was to investigate the effect of HIIT in fitness, in addition to checking the lactate curve and glycemic variation in maximal exercise test (MET). For this, used were 8 male Wistar rats on a treadmill adapted for rodents for two weeks. To determine the intensity of the animals were subjected to TEM before the start of training and subsequently refreshed every two weeks. It HAS consists of steps 3 minutes of running with constant load, followed by two minutes of passive rest. At each stage there was an increase of 0.3 km / h to exhaustion of the animal and determine the lactate threshold. Blood samples (25 µL) were performed during rest, with the help of capillary calibrated with EDTA. Immediately after collecting the blood was transferred to eppendorf tube containing 50 µL of sodium fluoride for subsequent analysis lactimeter YSI 2300 STAT plus. The glucose analysis pre and post TEM was performed with the aid of reactive tapes and analyzed Accu-Chek ACTIVE®. After a certain MET, the animals were submitted to HIIT, which consisted of five minutes of warming, followed by four shots at 80% of maximum capacity followed by passive rest, finishing with five minute cool down. This study was approved by the Ethics Committee on Animal Use (CEUA) of the State University of Ceará (UECE) by the number 2542310/2015. For analysis of the linearity of lactate curves was carried out Pearson's correlation for all MET. We used the analysis of variance (ANOVA - two way) followed by Bonferroni post test to check differences in pre and post glucose MET. In all cases the significance level was preset at p <0.05. An increase in the physical condition of the animals, where the maximum speed obtained in the first MET was 1.94 ± 0.12 and 3.95 ± 0.24 after 8 weeks of HIIT with Pearson linear correlation was significant in all MET . This is due to ATP production by anaerobic glycolytic system result in the accumulation of pyruvate and NADH in the muscle, which subsequently forms the lactate. In glycemic analysis it was found that in the first TEM there was a significant increase in postworkout blood glucose (pre=119±19, post=170±19), possibly by increased activation of the adrenergic system inducing hepatic glycogenolysis. In TEM 5 there was a significant reduction in blood glucose after TEM (pre=137±15, post=130±21) can be explained by increased phosphorylation of GLUT4, increasing glucose uptake by muscle exercise. Therefore, HIIT was able to increase physical ability of animals to give an increasing curve of lactate in all MET and distinct variations in glycaemia.(AU)

Assuntos

RESUMO

Alzheimer's disease (AD) was established as the neurodegenerative dementia, which leads to progressive and irreversible cognitive decline, memory loss, attention and judgment. It is characterized by molecular injuries resulting from the accumulation of charged neuritic plaques of β-amyloid and neurofibrillary tangles causing oxidative damage and inflammatory. Thus, the aim of this study was to evaluate the learning and memory of rats seven days after infusion of β-amiloide1-42. The β-amiloide1-42 was prepared in saline protein (1ug / uL) and incubated at 37 ° C for three days to form the aggregate. The animals were anesthetized with the combination of ketamine (100 mg / kg) and xylazine (10 mg / kg) intraperitoneally, and fixed in a stereotaxic route. The aggregate was infused directly in the hippocampus (5UL bilaterally) in the coordinates, AP: -3.5 mm; LL: ± 2.0 mm; DV: -3.5. The learning and memory was assessed using the Morris water maze. The apparatus consists of a circular water tank (120 cm diameter and 60 cm high) with an escape platform (12.5 cm in diameter and 38 cm high) positioned invisible to the mice 2 cm below the water level. The tank was divided by imaginary lines (N, S, L, and O) in four quadrants (1, 2, 3 and 4) and identified by the same geometrical symbols, which remained the same throughout the study. The animals were placed facing the geometric shapes and the exhaust time measured during four consecutive days (training). In the fifth and final day it was taken to the escape platform and computed the total residence time in the target quadrant (test). The maximum time for each step was 60s. This study was approved by Ethics Committee for Animal Use (CEUA) of the State University of Ceará (UECE) by the number 2542310/2015. To analyze the learning we used the ANOVA two way with Bonferroni post-test. To verify differences in memory between the groups we used the Student t test. P <0.05 significance level was adopted[...](AU)

Assuntos

RESUMO

Alzheimer's disease (AD) was established as the neurodegenerative dementia, which leads to progressive and irreversible cognitive decline, memory loss, attention and judgment. It is characterized by molecular injuries resulting from the accumulation of charged neuritic plaques of β-amyloid and neurofibrillary tangles causing oxidative damage and inflammatory. Thus, the aim of this study was to evaluate the learning and memory of rats seven days after infusion of β-amiloide1-42. The β-amiloide1-42 was prepared in saline protein (1ug / uL) and incubated at 37 ° C for three days to form the aggregate. The animals were anesthetized with the combination of ketamine (100 mg / kg) and xylazine (10 mg / kg) intraperitoneally, and fixed in a stereotaxic route. The aggregate was infused directly in the hippocampus (5UL bilaterally) in the coordinates, AP: -3.5 mm; LL: ± 2.0 mm; DV: -3.5. The learning and memory was assessed using the Morris water maze. The apparatus consists of a circular water tank (120 cm diameter and 60 cm high) with an escape platform (12.5 cm in diameter and 38 cm high) positioned invisible to the mice 2 cm below the water level. The tank was divided by imaginary lines (N, S, L, and O) in four quadrants (1, 2, 3 and 4) and identified by the same geometrical symbols, which remained the same throughout the study. The animals were placed facing the geometric shapes and the exhaust time measured during four consecutive days (training). In the fifth and final day it was taken to the escape platform and computed the total residence time in the target quadrant (test). The maximum time for each step was 60s. This study was approved by Ethics Committee for Animal Use (CEUA) of the State University of Ceará (UECE) by the number 2542310/2015. To analyze the learning we used the ANOVA two way with Bonferroni post-test. To verify differences in memory between the groups we used the Student t test. P <0.05 significance level was adopted[...]

Assuntos

RESUMO

The high-intensity interval training (HIIT) is characterized by vigorous and intermittent performing certain exercise separated by periods of passive or active rest with low intensity. This training model can be as effective as continuous endurance training. However, the aim of this study was to investigate the effect of HIIT in fitness, in addition to checking the lactate curve and glycemic variation in maximal exercise test (MET). For this, used were 8 male Wistar rats on a treadmill adapted for rodents for two weeks. To determine the intensity of the animals were subjected to TEM before the start of training and subsequently refreshed every two weeks. It HAS consists of steps 3 minutes of running with constant load, followed by two minutes of passive rest. At each stage there was an increase of 0.3 km / h to exhaustion of the animal and determine the lactate threshold. Blood samples (25 µL) were performed during rest, with the help of capillary calibrated with EDTA. Immediately after collecting the blood was transferred to eppendorf tube containing 50 µL of sodium fluoride for subsequent analysis lactimeter YSI 2300 STAT plus. The glucose analysis pre and post TEM was performed with the aid of reactive tapes and analyzed Accu-Chek ACTIVE®. After a certain MET, the animals were submitted to HIIT, which consisted of five minutes of warming, followed by four shots at 80% of maximum capacity followed by passive rest, finishing with five minute cool down. This study was approved by the Ethics Committee on Animal Use (CEUA) of the State University of Ceará (UECE) by the number 2542310/2015. For analysis of the linearity of lactate curves was carried out Pearson's correlation for all MET. We used the analysis of variance (ANOVA - two way) followed by Bonferroni post test to check differences in pre and post glucose MET. In all cases the significance level was preset at p <0.05. An increase in the physical condition of the animals, where the maximum speed obtained in the first MET was 1.94 ± 0.12 and 3.95 ± 0.24 after 8 weeks of HIIT with Pearson linear correlation was significant in all MET . This is due to ATP production by anaerobic glycolytic system result in the accumulation of pyruvate and NADH in the muscle, which subsequently forms the lactate. In glycemic analysis it was found that in the first TEM there was a significant increase in postworkout blood glucose (pre=119±19, post=170±19), possibly by increased activation of the adrenergic system inducing hepatic glycogenolysis. In TEM 5 there was a significant reduction in blood glucose after TEM (pre=137±15, post=130±21) can be explained by increased phosphorylation of GLUT4, increasing glucose uptake by muscle exercise. Therefore, HIIT was able to increase physical ability of animals to give an increasing curve of lactate in all MET and distinct variations in glycaemia.

Assuntos