RESUMO
New therapeutic approaches are necessary to control strongyloidiasis due to the side effects of, and resistance to, currently available drugs thiabendazole, albendazole, and ivermectin. This study examined the anthelmintic properties of extracts and isolated compounds from Siparuna guianensis against Strongyloides venezuelensis eggs and larvae, using the egg hatching test (EHT) and larval motility test (LMT). Albendazole (0.025 mg/ml) and ivermectin (0.316 mg/ml) were used as the positive controls for the EHT and LMT assays, respectively. Strongyloides venezuelensis eggs or larvae (±50 specimens) were treated with ethanol extract (0.05-1.0 mg/ml), ethyl acetate and aqueous fractions (0.05-0.8 mg/ml), essential oil (0.2-1.0 mg/ml) and α-bisabolol (0.2-1.0 mg/ml) from S. guianensis, and analysed by optical microscopy after 48 h (EHT), or after 24, 48 and 72 h (LMT). All the tested compounds exhibited ovicidal activity equivalent to the positive control and changed the morphology of the eggs. The S. guianensis ethanol extract and aqueous fraction were as effective as the positive control. Phytochemical analysis of the ethanol extract and fractions revealed the presence of phenolic compounds, tannins and flavonoids. Therefore, S. guianensis is effective against S. venezuelensis eggs and larvae in vitro, and can be considered as a potential alternative treatment for strongyloidiasis.
Assuntos
Anti-Helmínticos/farmacologia , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Strongyloides/efeitos dos fármacos , Traqueófitas/química , Animais , Anti-Helmínticos/química , Larva/efeitos dos fármacos , Sesquiterpenos Monocíclicos/química , Sesquiterpenos Monocíclicos/farmacologia , Óleos Voláteis/química , Óvulo/efeitos dos fármacos , Extratos Vegetais/químicaRESUMO
In broiler feed manufacturing, grinding may significantly increase costs due to electric energy use. This study evaluated the performance and the morphometrics of the gastrointestinal tract of broilers. According to a completely randomized experimental design, 2,400 birds (1,200 males and 1,200 females) were submitted to five treatments of eight replicates each. Treatments consisted of diets based corn or sorghum as follows: ground corn (A), broken corn (B), ground sorghum (C), ground (50%) and whole (50%) sorghum (D) and whole sorghum (100%; E). Pre-starter feeds (1 to 8 days), based on corn or sorghum, were produced only with ground grains. The study was divided in two trials: males and females. The results were submitted to analysis of variance (p 0.05) and Tukey's test. Feed intake, live weight, feed conversion ratio, and livability of 21- and 42-day-old male and female broilers were not affected by the treatments. This demonstrates that broiler performance is not influenced by sorghum physical form and that broilers fed sorghum-based diets presented similar performance as those fed corn-based diets. Gizzard and small intestine were heavier when whole sorghum grains was included in the diet. It was concluded that whole sorghum grains can be included in broiler feeds with not detrimental effects on performance.
Assuntos
Animais , Galinhas/fisiologia , Galinhas/metabolismo , Ração Animal/análise , Sorghum/químicaRESUMO
In broiler feed manufacturing, grinding may significantly increase costs due to electric energy use. This study evaluated the performance and the morphometrics of the gastrointestinal tract of broilers. According to a completely randomized experimental design, 2,400 birds (1,200 males and 1,200 females) were submitted to five treatments of eight replicates each. Treatments consisted of diets based corn or sorghum as follows: ground corn (A), broken corn (B), ground sorghum (C), ground (50%) and whole (50%) sorghum (D) and whole sorghum (100%; E). Pre-starter feeds (1 to 8 days), based on corn or sorghum, were produced only with ground grains. The study was divided in two trials: males and females. The results were submitted to analysis of variance (p 0.05) and Tukey's test. Feed intake, live weight, feed conversion ratio, and livability of 21- and 42-day-old male and female broilers were not affected by the treatments. This demonstrates that broiler performance is not influenced by sorghum physical form and that broilers fed sorghum-based diets presented similar performance as those fed corn-based diets. Gizzard and small intestine were heavier when whole sorghum grains was included in the diet. It was concluded that whole sorghum grains can be included in broiler feeds with not detrimental effects on performance.(AU)
Assuntos
Animais , Sorghum/química , Ração Animal/análise , Galinhas/metabolismo , Galinhas/fisiologiaRESUMO
The present study, investigated the mechanisms involved in the immune responses of Major Histocompatibility Complex class I or class II knockout mice, following Strongyloides venezuelensis infection. Wild-type C57BL/6 (WT), MHC II(-/-) and MHC I(-/-) mice were individually inoculated with 3000 larvae (L3) of S. venezuelensis and sacrificed on days 1, 3, 5, 8, 13 and 21 post-infection (p.i.). Samples of blood, lungs and small intestines were collected. The tissue samples were stained with hematoxylin-eosin for the pathological analysis. The presence of the parasite was demonstrated by immunoperoxidase analysis. MHC II(-/-) mice presented a significantly higher number of adult worms recovered from the small intestine on day 5p.i. and presented elevated numbers of eggs in the feces. The infection by S. venezuelensis was completely eliminated 13 days after infection in WT as well as in MHC I(-/-) mice. In MHC II(-/-) mice, eggs and adult worms were still found on day 21 p.i., however, there was a significant reduction in their numbers. In the lung, the parasite was observed in MHC I(-/-) on day 1 p.i. and in MHC II(-/-) mice on days 1 and 5 p.i. In the small intestine of WT mice, a larger number of parasites were observed on day 8 p.i. and their absence was observed after day 13 p.i. Through immunohistochemistry analysis, the parasite was detected in the duodenum of WT on days 5 and 8 p.i., and in knockout mice on days 5, 8 and 13 p.i.; as well as in posterior portions of the small intestine in MHC I(-/-) and MHC II(-/-) on day 13 p.i., a finding which was not observed in WT mice. We concluded that immunohistochemistry analysis contributed to a more adequate understanding of the parasite localization in immunodeficient hosts and that the findings aid in the interpretation of immunopathogenesis in Strongyloides infection.
Assuntos
Genes MHC da Classe II/genética , Genes MHC Classe I/genética , Enteropatias Parasitárias/imunologia , Pneumopatias Parasitárias/imunologia , Estrongiloidíase/imunologia , Animais , Enteropatias Parasitárias/parasitologia , Enteropatias Parasitárias/patologia , Intestinos/patologia , Pulmão/patologia , Pneumopatias Parasitárias/parasitologia , Pneumopatias Parasitárias/patologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Strongyloides , Estrongiloidíase/parasitologia , Estrongiloidíase/patologiaRESUMO
Infection rates with Giardia duodenalis and Cryptosporidium spp. were compared among dogs living under different conditions. Stool samples (n = 433) collected from dogs of different ages, gender, living conditions and origin were analyzed using three techniques, i.e., centrifugal flotation in zinc sulfate solution, centrifugal flotation in sucrose solution, and methylene blue gram safranin staining. Eighty-nine of the samples were from stay dogs living in shelters run by animal protection societies, 199 were from kennels and 122 from households. A total of 119 (29.0%) had G. duodenalis cysts and six (1.4%) were positive for Cryptosporidium spp. oocysts. Dogs from kennels were most frequently affected by G. duodenalis (49.7%) while those from shelters showed a higher prevalence of Cryptosporidium spp. (2.2%). A significant difference (p < or = 0.05) was observed between immature dogs and adults only with respect to Giardia infection. There was no significant difference between the gender with regard to the presence of either protozoan.
Assuntos
Criptosporidiose/veterinária , Cryptosporidium/isolamento & purificação , Doenças do Cão/epidemiologia , Giardia/isolamento & purificação , Giardíase/veterinária , Criação de Animais Domésticos , Animais , Brasil/epidemiologia , Criptosporidiose/epidemiologia , Doenças do Cão/parasitologia , Cães , Feminino , Giardíase/epidemiologia , Masculino , PrevalênciaRESUMO
Tachyzoites of Toxoplasma gondii were located inside the nucleus of both skeletal muscle cells infected in vitro and peritoneal exudate cells collected from infected mouse in vivo. Ultrastructural analysis demonstrated that T. gondii invades the nucleus of host cells by the parasite apical region and with constriction of its body. We noted that the rhoptry, a secretory organelle of the parasite that is involved in the host cell invasion mechanism, was empty in the intranuclear T. gondii. The parasites were found in the nuclear matrix without evidence of the vacuolar membrane. Frequently, new parasites invaded host cell nucleus, which was already infected. The significance of this nuclear invasion could reflect an alternative route of T. gondii for its transitory survival or an escape mechanism from the host immune response during the in vivo infection (or both).
Assuntos
Núcleo Celular/parasitologia , Músculo Esquelético/parasitologia , Toxoplasma/ultraestrutura , Vacúolos/parasitologia , Animais , Núcleo Celular/patologia , Núcleo Celular/ultraestrutura , Células Cultivadas , Membranas Intracelulares/patologia , Membranas Intracelulares/ultraestrutura , Camundongos , Microscopia Eletrônica , Músculo Esquelético/patologia , Músculo Esquelético/ultraestrutura , Toxoplasma/fisiologia , Vacúolos/patologia , Vacúolos/ultraestruturaRESUMO
The objective of the present research is to identify the reasons for the scarce participation of nursing professionals in the Brazilian Association of Nursing (ABEn) and other professional associations. The data was collected from nurses in the city of Cascavel-Paraná and was analysed according to qualitative and quantitative methods. Results show that a small number of professionals take part in the events promoted by ABEn-Cascavel and that there is little interest in joining any professional association. 36.6%, of nurses interviewed showed interest in becoming members of ABEn. Thus, for these professionals ABEn should define strategies of inclusion. The data collected in this research, regarding the reasons for the participation of nurses in the association, has provided ABEN-Cascavel with information that can support its performance in the city, as well as help on the planning of new activities and policies such as the promotion of events and changes on the price of the tuition, which was pointed out as one of the main reasons for the scarce participation of nurses in the association.
Assuntos
Enfermagem , Sociedades de Enfermagem/estatística & dados numéricos , Atitude do Pessoal de Saúde , Brasil , Emprego/estatística & dados numéricos , Feminino , Humanos , Entrevistas como Assunto , Masculino , Estudos de Casos Organizacionais , Seleção de Pessoal/organização & administração , Sociedades de Enfermagem/economia , Fatores Socioeconômicos , Recursos HumanosRESUMO
This study aimed at reflecting about nursing as job by disconsidering the widely accepted conception that identifies it as an assistance activity for that conception might hinder actions towards coping with and changing work conditions. The study attempted to place in history the context in which the vocational/religious model was elaborated as well as to identify the manifestations of that conception in the discourse of Nursing freshmen and seniors, pointing out the fact that the teaching activity must be permeated by the conception of nursing/work.
Assuntos
Atitude do Pessoal de Saúde , Enfermagem , Prática Profissional , BrasilRESUMO
This study identifies how faculty nurses and clinical nurses experience the relationship between teaching and the labor market in Nursing. Data were collected by means of interviews and documentary analysis. Methodological and content categories were utilized for analysis. It became evident that teaching is far from the clinical practice and that it is based on contents which do not have correspondents; there is a phase displacement between what is taught in undergraduation and the incorporation of new technologies, practices and knowledge. Action towards reflections about nursing teaching in order to approach it to clinical work areas is pointed out.
Assuntos
Setor de Assistência à Saúde , Prática do Docente de Enfermagem , Teoria de EnfermagemRESUMO
Nursing care to the human being in psychical suffering is the theme of this investigation, developed in a psychiatric hospital, in the west region of the Paraná State. This is a qualitative research with the aim of finding out nurses' understanding about their work in psychiatric nursing. Authors used open interview to all the nurses of the institution. Then, data were grouped in thematic unities and analysed through the analysis of the content.