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INTRODUCTION: This ex vivo study evaluated the disinfecting and cleaning effects of root canal preparation using sodium hypochlorite irrigation with 3 different needle designs. METHODS: Mesial roots from extracted mandibular molars with Vertucci class II configuration were anatomically matched based on micro-computed tomography (micro-CT) analyses and distributed into 3 groups (n = 18/group). The canals were contaminated with a mixed bacterial culture for 30 days and then subjected to preparation using 2.5% sodium hypochlorite irrigation with open-ended flat needle (3 mm short of the working length - WL), closed-ended side-vented irrigation needle (1 mm short of the WL), or a closed-ended plastic needle with 2 lateral outlets (TruNatomy) (1 mm short of the WL). Bacteriological samples were taken from the canals before (S1) and after preparation (S2). After another micro-CT scan, the roots were sectioned and samples were also taken from the apical canal segment (S3). Bacterial reduction was assessed by quantitative real-time polymerase chain reaction. The amount of accumulated hard tissue debris was evaluated by micro-CT. RESULTS: A substantial reduction in bacterial counts was observed in all 3 groups when comparing S1 with S2 (99.95%, 98.93%, and 98.90% in the open-ended, closed-ended, and TruNatomy needle groups, respectively) (P < .01). No significant differences were observed between groups for bacterial reduction in the full and apical canal (P > .05). There were no intergroup differences in the amount of accumulated hard tissue debris either (P > .05). The open-ended needle group showed significantly more specimens with quantitative real-time polymerase chain reaction negative results for bacteria in S3 than TruNatomy (P < .05). CONCLUSION: When used up to an appropriate insertion depth, the 3 needle types result in similar antibacterial and cleaning performance, provided variables such as needle size and irrigant type, volume and flow rate are controlled. Exclusive analysis of the apical segment, including the isthmus, revealed that the open-ended needle yielded more cases negative for bacteria.

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The objective of this study was to evaluate microbial leakage by means of genome counts, through the implant-abutment interface in dental implants with different Morse taper abutments. Fifty-six samples were prepared and divided in four groups: CMC TB (14 Cylindrical Implants-14 TiBase Abutments), CMX TB (14 Conical Implants-14 TiBase Abutments), CMX PU (14 Conical Implants-14 Universal Abutment) and CMX U (14 Tapered Implants-14 UCLA Abutments). Assemblies had their interface submerged in saliva as the contaminant. Samples were subjected either to thermomechanical cycling (2 × 106 mechanical cycles with frequency of 5 Hz and load of 120 N simultaneously with thermal cycles of 5-55 °C) or thermal cycling (5-55 °C). After cycling, the contents from the inner parts of assemblies were collected and analyzed using the Checkerboard DNA-DNA hybridization technique. Significant differences in the total genome counts were found after both thermomechanical or thermal cycling: CMX U > CMX PU > CMX TB > CMC TB. There were also significant differences in individual bacterial counts in each of the groups (p < 0.05). Irrespective of mechanical cycling, the type of abutment seems to influence not only the total microbial leakage through the interface, but also seems to significantly reflect differences considering individual target species.

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PURPOSE: To evaluate the effect of toothbrushing with conventional and whitening dentifrices on the color difference (ΔE00), gloss (Δgloss), and surface roughness (SR) of stained stabilized zirconia with 5 mol% of yttrium oxide (5Y-TZP) after polishing or glazing. METHODS: Specimens were divided into four groups (n=20): C (control), S (staining), SG (staining and glazing) and SP (staining and polishing). 50,000 toothbrushing cycles were performed with conventional (n=10) and whitening (n= 10) dentifrice slurries. The ΔE00 and Δgloss were measured using a spectrophotometer and CIEDE2000 system while SR was measured by laser confocal microscope. The ΔE00 and Δgloss data were analyzed using 2-way ANOVA, and SR data were analyzed using the linear repeated measures model, with Bonferroni's complementary test (α= 0.05). RESULTS: The ΔE00 values were beyond the acceptability threshold and no differences were found among the groups. There was no difference among groups to Δgloss after toothbrushing with conventional dentifrice while SP presented the highest values of Δgloss after toothbrushing with whitening dentifrice. Conventional dentifrice decreased the SR of stained groups and whitening dentifrice decreased SR of S and SG. The toothbrushing with conventional and whitening dentifrices promoted color difference, but did not impair gloss and surface roughness of stained 5Y-TZP. CLINICAL SIGNIFICANCE: Monolithic zirconia has been routinely used for esthetic restorations, however the type of finishing procedures that is carried out on it must be taken into consideration, in addition to the fact that brushing can influence the color difference of the material as well as interfere with surface roughness and gloss.

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The aim of this study was to evaluate the mechanical behavior of UCLA and Mini-conical abutments for implants with Tri-channel connections regarding torque loss and vertical misfit. Twenty 3-element metal-ceramic fixed partial dentures (FPD) supported by 2 implants were manufactured and divided into 2 groups (n = 10): UCLA (group 1) and Mini-conical Abutments (group 2). The evaluation of torque loss was carried out before and after mechanical cycling, while the vertical fit was evaluated throughout the different stages of manufacturing the prostheses, as well pre- and postcycling (300,000 cycles, 30 N). Statistical analyses of torque loss and vertical misfit were performed using the linear mixed effects model. Both groups showed torque loss after mechanical cycling (P < .05); however, there was no significant percentage differences between them (P = .795). Before cycling, the groups showed a significant difference in terms of vertical misfit values (P < .05); however, this difference was no long observed after cycling (P = .894). Both groups showed torque loss after the cycling test, with no significant difference (P > .05). There was no significant difference in vertical misfit after mechanical cycling; however, in group 1 (UCLA) there was accommodation of the implant-UCLA abutment interface, while group 2 (Mini-conical abutment) did not show changes in the interface with the implant after the test. Both groups behaved similarly regarding the torque loss of the prosthesis retention screws pre- and postmechanical cycling, with greater loss after the test.

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The use of bleaching agents to remove stains is one of the main dental procedures to improve the aesthetics of teeth. This review presents the main agents used for tooth whitening, existing clinical protocols, and the structural changes that may occur through their use. The main bleaching agents consist of hydrogen peroxide and carbamide peroxide, which are used in bleaching techniques for vital teeth. These techniques can be performed in the office by a professional or by the individual in a home en-vironment under professional guidance. Bleaching agents come in a variety of concentrations and there are over-the-counter products available on the market with lower concentrations of hydrogen peroxide. Due to the chemical characteristics of the agents, changes in the organic and inorganic content of the tooth structure can be observed. These changes are related to morphological changes characterized by in-creased permeability and surface roughness, such changes compromise the mechanical resistance of the tooth. Furthermore, bleaching agents can promote molecular changes after reaching the dental pulp, resulting in oxidative stress of pulp cells and the release of pro-inflammatory mediators. Despite the bleaching effectiveness, tooth sensitivity is considered the main side effect of use. Therefore, among the heterogeneity of protocols, those that used the bleaching agent for a prolonged time and in lower con-centrations presented more harmful effects on the tooth structure.

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INTRODUCTION: This study was intended to evaluate the expression of inflammatory cytokines commonly secreted by CD4+ T cells (IL-2, IL-5, IL-17, TGF-ß, TNF-α, and IFN-γ) in apical granulomas and correlate with the clinical conditions and time elapsed since root canal treatment. METHODS: Eighteen biopsy specimens obtained by periradicular surgery of teeth with post-treatment apical periodontitis and diagnosed as apical granuloma were available from the oral pathology laboratory. Silanized slides containing paraffin sections were used for immunohistochemical reactions. Images were analyzed by using an optical microscopy and each slide was subdivided into 5 fields at high magnification. RESULTS: IFN-γ and TGF-ß were the cytokines with the highest expression levels. There were statistically significant differences when comparing IL-2 and IFN-γ (P < .05), and IL-2 and TGF-ß (P < .05). Comparison between the detected cytokines and clinical data and time of treatment demonstrated significant correlation (P < .05) between lower expression of IL-2 and the presence of painful symptoms, absence of sinus tract, and treatments performed more than 4 years before. It was also possible to observe a significant correlation between lower expression of IL-5 and treatments performed less than 4 years before (P < .05). CONCLUSION: IFN-γ and TGF-ß were highly expressed in apical granulomas. However, only IL-2 and IL-5 levels were associated with clinical data and time since previous root canal treatment.

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Ultraviolet A (UVA) radiation, present in sunlight, can induce cell redox imbalance leading to cellular damage and even cell death, compromising skin health. Here, we evaluated the in vitro antioxidant and photochemoprotective effect of dithiothreitol (DTT). DTT neutralized the free radicals 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS·+), 2,2-diphenyl-1-picrylhydrazyl (DPPH·), and superoxide anion (O2·-) in in vitro assays, as well as the ferric ion (Fe3+) in the ferric reducing antioxidant power (FRAP) assay. We also evaluated the effect of DTT pre-treatment in L929 dermal fibroblasts and DTT (50 and 100 µM) led to greater cell viability following UVA-irradiation compared to cells that were untreated. Furthermore, the pre-treatment of cells with DTT prevented the increase of intracellular reactive oxygen species (ROS) production, including hydrogen peroxide (H2O2), lipid peroxidation, and DNA condensation, as well as the decrease in mitochondrial membrane potential (Δψm), that occurred following irradiation in untreated cells. The endogenous antioxidant system of cells was also improved in irradiated cells that were DTT pre-treated compared to the untreated cells, as the activity of the superoxide dismutase (SOD) and catalase (CAT) enzymes remained as high as non-irradiated cells, while the activity levels were depleted in the untreated irradiated cells. Furthermore, DTT reduced necrosis in UVA-irradiated fibroblasts. Together, these results showed that DTT may have promising use in the prevention of skin photoaging and photodamage induced by UVA, as it provided photochemoprotection against the harmful effects of this radiation, reducing oxidative stress and cell death, due mainly to its antioxidant capacity.

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OBJETIVO: Realizar a análise do conceito de abuso sexual em crianças, identificando os possíveis atributos, antecedentes e consequências. MÉTODO: Foi conduzida uma análise de conceito, estruturada por meio de uma scoping review. A busca foi realizada em 6 bases de dados e, após análise, um total de 17 artigos foram incluídos. RESULTADOS: Foram identificados, como antecedentes, crianças com idade acima de 8 anos, do sexo feminino, sendo o agressor frequentemente um parente da vítima ou até mesmo o próprio pai. Quanto aos atributos, foram identificadas alterações psicológicas, doenças sexualmente transmissíveis e gravidez indesejada. As consequências observadas foram predominantemente de natureza psicológica, incluindo depressão e comportamento suicida. CONCLUSÃO: No total, foram agrupados nove antecedentes que estão relacionados aos fatores que levam à vulnerabilidade do abuso. Além disso, foram identificados 11 atributos correspondentes aos sinais característicos que indicam que uma criança é vítima de abuso. Por fim, foram observadas 12 consequências decorrentes da violência sexual infantil.

OBJECTIVE: To conduct a conceptual analysis of child sexual abuse to identify possible attributes, antecedents, and consequences. METHOD: A concept analysis was conducted using a scoping review methodology. Searches were conducted in 6 databases, resulting in 17 articles after analysis. RESULTS: Antecedents included children older than 8 years, female gender, the perpetrator often being a relative of the victim or even the biological father. Psychological changes, sexually transmitted diseases, and unwanted pregnancies were identified. The consequences observed were mainly psychological, including depression and suicidal behavior. CONCLUSIONS: Nine antecedents were grouped as factors contributing to vulnerability to abuse. In addition, 11 attributes correspond to characteristic signs indicating that a child is a victim of maltreatment. Finally, 12 consequences of child sexual violence were observed.

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Resumo: Este artigo reflete sobre a singularidade das relações entre mulheres rurais e os cães que com elas constituem um relacionamento interespecífico. Objetiva contar sobre práticas que foram emergindo ao longo de uma pesquisa quando passamos a encarar seriamente a agência de animais a partir do cotidiano de uma agricultora pesquisadora alinhada à agroecologia familiar. Focalizamos os cães de passagem sem raça definida e que vão e vêm livremente entre as cercas que delimitam as propriedades rurais. Em diálogo com Donna Haraway, Anna Tsing e Vinciane Despret, apresentamos pistas para uma pesquisa multiespécie que opera por meio de causos. A pesquisa multiespécie nos convida a reflexões sobre práticas anticapitalistas que germinam na agroecologia, a nos determos no trabalho das mulheres agricultoras e dos cães na sua potência de autogoverno e de criarmos formas de cooperação multiespecíficas com alteridades significativas no projeto moderno-colonizador.

Resumen: Este artículo reflexiona sobre la singularidad de las relaciones entre las mujeres rurales y los perros que constituyen una relación interespecífica con ellas. El objetivo es relatar las prácticas que surgieron a lo largo de una investigación cuando empezamos a considerar seriamente la agencia animal basada en la vida diária de una agricultora investigadora alineada con la agroecología familiar. Centramonos en perros mestizos que van y vienen libremente entre las vallas que delimitan las propiedades rurales. En diálogo con Donna Haraway, Anna Tsing y Vinciane Despret, presentamos pistas para una investigación multiespecie que opera a través de historias. La investigación multiespecie nos invita a reflexionar sobre las prácticas anticapitalistas que germinan en la agroecología, a centrarnos en el trabajo de las campesinas y los perros en su poder de autogobierno y a crear formas de cooperación multiespecífica con alteridades significativas en el proyecto moderno-colonizador.

Abstract: This article reflects on the uniqueness of the relationships between rural women and the dogs that constitute an interspecific relationship with them. It aims to tell about practices that emerged throughout research when we began to seriously consider the animal agency based on the daily life of a farmer researcher aligned with family agroecology. We focus on mixed breed dogs that come and go freely between the fences that delimit rural properties. In dialogue with Donna Haraway, Anna Tsing and Vinciane Despret, we present clues for multispecies research that operates through stories. Multispecies research invites us to reflect on anti-capitalist practices that germinate in agroecology, to focus on the work of women farmers and dogs in their power of self-government and to create forms of multispecific cooperation with significant alterities in the modern-colonizing project.

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RESUMO Objetivo: identificar os marcadores de violência contra pessoa idosa na perspectiva de enfermeiros. Método: estudo qualitativo conduzido no estado da Paraíba - Brasil, com a participação de nove enfermeiros, em dois grupos focais, durante junho e setembro de 2021. O material empírico foi transcrito na íntegra, transformado em corpus textual com análise de similitude. Resultados: no núcleo central da árvore máxima foi possível observar termos comuns a mais de uma tipificação de violência: "ciclo de violência", "confiança", "dominar", "sofrimento psíquico". Na violência física, "fraturas", "quebrar" e "jogar"; na psicológica, "ameaçar", "medo", "vergonha", "lesão", "corrimento vaginal" e "vaginal"; na financeira, "saque", "dependência" e "pegar"; na negligência, "falta", "insumo" e "higiene"; no abandono, "depressão", "solidão" e "tristeza". Conclusão: esta pesquisa valoriza a Enfermagem como ciência e profissão, especialmente na área forense, ao investigar a violência contra a pessoa idosa. A percepção do enfermeiro torna o processo de enfermagem mais confiável e útil na identificação, notificação e resolução das ocorrências.

ABSTRACT Objective: to identify the markers of violence against older adults from the nurses' perspective. Method: a qualitative study conducted between June and September 2021 in the state of Paraíba (Brazil), with the participation of nine nurses in two focus groups. The empirical material was transcribed in full and transformed into a text corpus with similarity analysis. Results: in the central core of the maximum tree, it was possible to observe terms common to more than one typification of violence, such as: "cycle of violence", "trust", "dominate", "psychological distress". In physical violence: "fractures", "break" and "throw"; in psychological violence: "threat", "fear", "shame", "injury", "vaginal discharge" and "vaginal"; in financial violence: "withdrawal", "dependence" and "take"; in neglect: "lack", "supply" and "hygiene"; and in abandonment: "depression", "loneliness" and "sadness". Conclusion: his research values Nursing as a science and profession, especially in the forensic area, by investigating violence against older adults. The nurses' perception makes the Nursing Process more reliable and useful in identifying, reporting and solving incidents.

RESUMEN Objective: identificar marcadores de violencia contra las personas mayores desde la perspectiva de los enfermeros. Método: estudio cualitativo realizado en el estado de Paraíba, Brasil, con nueve enfermeros, por medio de dos grupos focales, durante junio y septiembre de 2021. El material empírico fue transcrito de forma completa, transformado en corpus textual y sometido a análisis de similitud. Resultados: en el núcleo central del árbol máximo fue posible observar términos comunes a más de un tipo de violencia: "ciclo de violencia", "confianza", "dominar", "sufrimiento psíquico". Los términos relacionados con la violencia física fueron, "fracturas", "romper" y "arrojar"; con la psicológica, "amenazar", "miedo", "vergüenza", "lesión", "secreción vaginal" y "vaginal"; con la financiera, "extracción", "dependencia" y "agarrar"; con la negligencia, "falta", "insumo" e "higiene"; con el abandono, "depresión", "soledad" y "tristeza". Conclusión: esta investigación enaltece la Enfermería como ciencia y profesión, especialmente en el área forense, al investigar la violencia contra las personas mayores. La percepción del enfermero hace que el proceso de enfermería sea más confiable y útil para identificar, informar y resolver casos.