RESUMO

Myrciaria dubia is a native plant from the Amazon region which produces red-purplish fruit rich in antioxidant compounds such as ascorbic acid, carotenoids, and phenolic. M. dubia fruit is used to prepare juices considered to possess high nutritional content providing health benefits. The aim of this study was to examine the ability of M. dubia juice to protect DNA against genomic instability induced by sub-acute ethanol consumption attributed to oxidative stress. Mice were treated for 28 days with juice at 25% and 50% diluted in distilled water or with the diluted combination juice plus ethanol (5 g/kg). The genotoxic/antigenotoxic and mutagenic/antimutagenic effects were assessed using comet assay in blood, liver, and kidney and micronucleus (MN) test with bone marrow. In addition, the mutagenicity was also evaluated using Salmonella/microsome assay. Phytochemical compounds were determined using HPLC/PDA/MS/MS. The juice did not induce genotoxic effects in blood, kidney, and liver cells at both doses. In combination with ethanol, the juice reduced the alcohol-mediated DNA damage in all tissues analyzed. Further, the juice did not produce mutagenic effects and decreased mutagenicity induced by ethanol in the bone marrow. The anthocyanins were major compounds detected by HPLC/PDA/MS/MS, which modulated genotoxic and mutagenic effects initiated by ethanol and at least in part appeared responsible for the observed antigenotoxic and antimutagenic effects of M. dubia juice.

Assuntos

RESUMO

O objetivo deste trabalho foi verificar a eficiência do sistema de Análise de Perigos e Pontos Críticos de Controle (APPCC) implantado em indústria de laticínios através do monitoramento dos Pontos Críticos de Controle (PCC) identificados no plano APPCC para a produção do leite longa vida. Foram avaliados os processos produtivos de três caminhões. No PCC-1 realizaram-se testes de acidez titulável, alizarol, fosfatase alcalina, peroxidase e análise de resíduos de antibióticos. No PCC-2 foram analisadas a relação de tempo/temperatura da carta gráfica, bem como as análises de fosfatase alcalina e peroxidase com o intuito de avaliar a eficiência dos processos. No PCC-3 realizaram-se pesquisas de micro-organismos aeróbios mesófilos em três caixas de leite longa vida após incubação de sete dias. Um total de 9 (100%) das amostras indicaram acidez Dornic entre 0,14 e 0,16ºD; 9 (100%) apresentaram resultado negativo ao teste de alizarol; e 9 (100%) mostraram resultado negativo para resíduos de antibióticos. As curvas gráficas mantiveram-se em seu tempo/temperatura correto, encontraram-se entre 74.1ºC-74.2ºC/15-20seg, o leite proveniente de cada caminhão apresentou resultado positivo para as enzimas fosfatase e peroxidase no leite cru, e após as pasteurizações o resultado foi negativo para enzima fosfatase e positivo para peroxidase. As amostras referentes a cada caminhão apresentaram resultado semelhante de <1,0x10º UFC/ml. Os resultados obtidos em cada PCC foram satisfatórios de acordo com a legislação pertinente. Os PCC estão dentro do estabelecido, demonstrando sua efetividade na aplicação do sistema APPCC na indústria de laticínios.(AU)

To verify the efficiency of the Hazard Analysis and Critical Control Points (HACCP) system implemented in the dairy industry by monitoring the Critical Control Points (CCPs) identified in the HACCP plan for the production of long-life milk. The production process of three trucks was evaluated in the PCC-1 titratable acidity tests, alizarol, alkaline phosphatase, peroxidase and antibiotic residue analysis. In PCC-2, the time / temperature relationship of the chart was analyzed, as well as the alkaline phosphatase and peroxidase analyzes in order to evaluate the efficiency of the processes. In PCC-3, we performed surveys of aerobic mesophilic microorganisms in three boxes of long-life milk after incubation for seven days. A total of 9 (100%) of the samples indicated Dornic acidity between 0.14 and 0.16ºD, 9 (100%) presented negative results to the alizarol test and 9 (100%) showed negative results for antibiotic residues. The graphical curves were maintained at their correct temperature / time, between 74.1ºC-74.2ºC / 15-20sec, the milk from each truck presented a positive result for the enzymes phosphatase and peroxidase in the raw milk, after the pasteurisations. Result was negative for enzyme phosphatase and positive for peroxidase. Samples from each truck had a similar result of <1.0x10º CFU / ml. The results obtained in each CCP were satisfactory according to the relevant legislation. The CCPs are within the established, demonstrating their effectiveness in the application of the HACCP system in the dairy industry.(AU)

Assuntos

RESUMO

O objetivo deste trabalho foi verificar a eficiência do sistema de Análise de Perigos e Pontos Críticos de Controle (APPCC) implantado em indústria de laticínios através do monitoramento dos Pontos Críticos de Controle (PCC) identificados no plano APPCC para a produção do leite longa vida. Foram avaliados os processos produtivos de três caminhões. No PCC-1 realizaram-se testes de acidez titulável, alizarol, fosfatase alcalina, peroxidase e análise de resíduos de antibióticos. No PCC-2 foram analisadas a relação de tempo/temperatura da carta gráfica, bem como as análises de fosfatase alcalina e peroxidase com o intuito de avaliar a eficiência dos processos. No PCC-3 realizaram-se pesquisas de micro-organismos aeróbios mesófilos em três caixas de leite longa vida após incubação de sete dias. Um total de 9 (100%) das amostras indicaram acidez Dornic entre 0,14 e 0,16ºD; 9 (100%) apresentaram resultado negativo ao teste de alizarol; e 9 (100%) mostraram resultado negativo para resíduos de antibióticos. As curvas gráficas mantiveram-se em seu tempo/temperatura correto, encontraram-se entre 74.1ºC-74.2ºC/15-20seg, o leite proveniente de cada caminhão apresentou resultado positivo para as enzimas fosfatase e peroxidase no leite cru, e após as pasteurizações o resultado foi negativo para enzima fosfatase e positivo para peroxidase. As amostras referentes a cada caminhão apresentaram resultado semelhante de <1,0x10º UFC/ml. Os resultados obtidos em cada PCC foram satisfatórios de acordo com a legislação pertinente. Os PCC estão dentro do estabelecido, demonstrando sua efetividade na aplicação do sistema APPCC na indústria de laticínios.

To verify the efficiency of the Hazard Analysis and Critical Control Points (HACCP) system implemented in the dairy industry by monitoring the Critical Control Points (CCPs) identified in the HACCP plan for the production of long-life milk. The production process of three trucks was evaluated in the PCC-1 titratable acidity tests, alizarol, alkaline phosphatase, peroxidase and antibiotic residue analysis. In PCC-2, the time / temperature relationship of the chart was analyzed, as well as the alkaline phosphatase and peroxidase analyzes in order to evaluate the efficiency of the processes. In PCC-3, we performed surveys of aerobic mesophilic microorganisms in three boxes of long-life milk after incubation for seven days. A total of 9 (100%) of the samples indicated Dornic acidity between 0.14 and 0.16ºD, 9 (100%) presented negative results to the alizarol test and 9 (100%) showed negative results for antibiotic residues. The graphical curves were maintained at their correct temperature / time, between 74.1ºC-74.2ºC / 15-20sec, the milk from each truck presented a positive result for the enzymes phosphatase and peroxidase in the raw milk, after the pasteurisations. Result was negative for enzyme phosphatase and positive for peroxidase. Samples from each truck had a similar result of <1.0x10º CFU / ml. The results obtained in each CCP were satisfactory according to the relevant legislation. The CCPs are within the established, demonstrating their effectiveness in the application of the HACCP system in the dairy industry.

Assuntos

RESUMO

O número de pessoas que realizam suas refeições em restaurantes tem aumentado exponencialmente, sendo preocupante a qualidade dos alimentos servidos, uma vez que, quando contaminados, podem acarretar graves surtos de Doenças Transmitidas por Alimentos. O presente estudo teve por objetivo analisar a presença de Salmonella spp. e Listeria monocytogenes em saladas contendo maionese servidas em restaurantes no município de Ji-Paraná, Rondônia. Foram coletadas 20 amostras de cinco restaurantes, sendo 4 amostras de cada estabelecimento. Do total de amostras analisadas, 30% apresentaram contaminação por Salmonella spp., não sendo constatada presença de Listeria monocytogenes. A contaminação por Salmonella spp. demonstra a necessidade de maior controle de qualidade no preparo desse alimento e fiscalização por parte dos órgãos responsáveis, tendo em vista o fato deste microrganismo ser o mais associado a surtos de Doenças Transmitidas por Alimento

The number of people eating in restaurants has increased, which concerns the quality of food served, since contaminated food can cause serious foodborne disease outbreaks. This study aimed to analyze the presence of Salmonella spp. and Listeria monocytogenes in salads containing mayonnaise served in restaurants in the city of Ji-Paraná, Rondônia. Twenty samples were collected in five restaurants, 4 from each establishment. Of the total, 30% were contaminated by Salmonella spp., while no contamination was found for Listeria monocytogenes. The contamination by Salmonella spp. demonstrates the need for greater quality control in food preparation, and supervision by the responsible agencies, once Salmonella is the microorganism most associated with foodborne disease outbreaks.