RESUMO
Liver transplantation is the only therapeutic option for patients with end-stage liver disease. Nitric oxide, a free radical produced from L-arginine, a potent vasodilator, also inhibits platelet adhesion and aggregation, reduces adhesion of leukocytes to the endothelium and suppresses proliferation of vascular smooth muscle cells. The inducible form of the nitric oxide synthase may generate large quantities of nitric oxide, and may be induced by the action of cytokines and lipopolysaccharides. Nitric oxide can be released from the hepatic vascular endothelium, platelets and Kupffer cells as a response to ischemia-reperfusion injury and circulatory shock. We analyzed the relationships between the levels of nitric oxide, hepatic enzymes and other clinical parameters (glucose, total proteins, total bilirubin, creatinine, albumin) obtained in serum samples before liver transplantation and every 48 h till day 15 in 15 patients aged 40 +/- 13 years. Aspartate aminotransferase and alanine aminotransferase levels changed from high at the beginning, to almost normal at the end of the study, cholinesterase levels remained decreased throughout the study and nitric oxide remained high, never reaching normal values.
Assuntos
Transplante de Fígado/fisiologia , Óxido Nítrico/sangue , Adolescente , Adulto , Alanina Transaminase/sangue , Aspartato Aminotransferases/sangue , Bilirrubina/sangue , Glicemia/metabolismo , Proteínas Sanguíneas/metabolismo , Colinesterases/sangue , Creatinina/sangue , Feminino , Humanos , Fígado/enzimologia , Falência Hepática/sangue , Falência Hepática/cirurgia , Masculino , Pessoa de Meia-Idade , Óxido Nítrico Sintase/sangue , Óxido Nítrico Sintase Tipo II , Albumina Sérica/metabolismoRESUMO
We have developed a quick and reliable diagnostic method for detecting variant forms of transthyretin (TTR); namely, centrifugal concentration followed by electrospray ionization mass spectrometry (ESI-MS). Argentinian patients from three families with neuropathic amyloidosis and their relatives were screened for mutated TTR by ESI-MS. In order to facilitate transportation, we investigated the impact storage had on lyophilized anti-TTR-antibody precipitates' mass spectra. For this investigation, plasma samples from three Swedish patients with known TTR amyloidosis were analysed. We detected identical, additional peaks corresponding to a variant form of TTR in 10 members of the families, and also in a lyophilized sample sent unfrozen by mail from Argentina. All except one symptomatic subject had additional peaks, the exception having undergone a liver transplantation for the disease. All patients were early onset cases, i.e. below 35 years of age, and family history suggests an aggressive, rapidly progressing disease. Lyophilized anti-TTR-antibody precipitates stored at room temperature for 1 week exhibited only minor differences compared with plasma samples stored at -70 degrees C. In a new Argentinian study on familial amyloidotic polyneuropathy, the variant TTR was quickly identified and typed by ESI-MS. To facilitate transportation, dry-frozen samples can be used and the quality of the spectra is similar to that of samples stored at -70 degrees C.
Assuntos
Neuropatias Amiloides/diagnóstico , Mutação , Pré-Albumina/análise , Adolescente , Adulto , Neuropatias Amiloides/sangue , Neuropatias Amiloides/genética , Argentina/etnologia , Preservação de Sangue , Feminino , Humanos , Masculino , Espectrometria de Massas , Pré-Albumina/genética , Manejo de EspécimesAssuntos
Ácido Hialurônico/análise , Transplante de Fígado/fisiologia , Adenosina , Alopurinol , Biomarcadores/análise , Glutationa , Humanos , Insulina , Fígado , Transplante de Fígado/métodos , Preservação de Órgãos , Soluções para Preservação de Órgãos , Valor Preditivo dos Testes , Rafinose , Reperfusão , Resultado do TratamentoAssuntos
Rejeição de Enxerto/urina , Transplante de Rim/fisiologia , Urina/química , Biópsia , Rejeição de Enxerto/tratamento farmacológico , Rejeição de Enxerto/patologia , Humanos , Concentração de Íons de Hidrogênio , Imunossupressores/uso terapêutico , Transplante de Rim/imunologia , Transplante de Rim/patologiaRESUMO
To determine if there are differences in urinary glycosaminoglycan (GAG) concentrations, 43 stone-forming patients and 37 healthy control subjects of both sexes were studied. Urinary concentrations of calcium, magnesium, creatinine, uric acid and GAGs were determined. GAGs were measured by the Di Ferrante precipitation procedure followed by the Bitter and Muir reaction. Urinary GAG concentration and daily output were significantly lower in stone-forming patients. The present study clearly demonstrates the decreased urinary GAG concentration and excretion in stone-forming patients and suggests an interaction between GAGs and urate that could modify the inhibitory potency of GAGs.