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Introducción: La enfermedad del Chikungunya es una arbovirosis, producida por el virus Alfavirus de la familia Togaviridae, transmitida por la picadura de mosquitos Aedes aegypti o Aedes albopictus. Objetivo: Determinar las características clínicas y la calidad de vida posterior a la infección por el virus de Chikungunya (CHIKV). Materiales y Métodos: estudio observacional, descriptivo, de corte transversal, de pacientes con CHIKV durante el mes de agosto de 2023. Se realizó un cuestionario, se midieron datos sociodemográficos, al diagnóstico de infección por CHIKV, duración de la enfermedad y manifestaciones clínicas. Se incluyó la versión española del "Cuestionario de calidad de vida SF-36". Resultados: Participaron 192 personas que tuvieron chikungunya. La edad promedio de la población de estudio fue 36 +/- 13,62 años. El 53,65% fue del sexo femenino. Con respecto al estado de salud, tenían un peor estado de salud las mujeres (M: 68.93% vs H: 55,06%), empeorando con el aumento de la edad. Los síntomas más frecuentes fueron la fiebre y las poliartralgias. Conclusión: Todos los individuos presentan algún tipo de afectación en la calidad de vida, siendo más notorio en los dominios de rol físico, vitalidad y dolor.
Introduction: Chikungunya disease is an arboviroais, caused by an Alfavirus virus of the Togaviridae family, transmitted by Aedes aegypti or Aedes albopictus mosquitoes. Objective: To determine the clinical characteristics and quality of life after Chikungunya virus (CHIKV) infection. Materials and Methods: observational, descriptive, cross-sectional study of patients with CHIKV during the month of August 2023. A questionnaire was carried out, sociodemographic data, diagnosis of CHIKV infection, duration of the disease and clinical manifestations were measured. The Spanish version of the "SF-36 Quality of Life Questionnaire" was included. Results: A group of 192 patients was evaluated during the study period. The average age of the study population was 36 +/- 13.62 years and 53.65% were female. Women reported worse health status (M: 68.93% vs. M: 55.06%) in general, and this increased with age. The most frequent symptoms were fever and polyarthralgia. Conclusion: All individuals reported some type of impairment in quality of life, particularly in the domains of physical role, vitality and pain.
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Porcine epidemic diarrhea virus (PEDV) has affected the pork industry worldwide and during outbreaks the mortality of piglets has reached 100%. Lipid nanocarriers are commonly used in the development of immunostimulatory particles due to their biocompatibility and slow-release delivery properties. In this study, we developed a lipid nanoparticle (LNP) complex based on glycyrrhizinic acid (GA) and tested its efficacy as an adjuvant in mice immunized with the recombinant N-terminal domain (NTD) of porcine epidemic diarrhea virus (PEDV) spike (S) protein (rNTD-S). The dispersion stability analysis (Z-potential -27.6 mV) confirmed the size and charge stability of the LNP-GA, demonstrating that the particles were homogeneously dispersed and strongly anionic, which favors nanoparticles binding with the rNTD-S protein, which showed a slightly positive charge (2.11 mV) by in silico analysis. TEM image of LNP-GA revealed nanostructures with a spherical-bilayer lipid vesicle (~100 nm). The immunogenicity of the LNP-GA-rNTD-S complex induced an efficient humoral response 14 days after the first immunization (p < 0.05) as well as an influence on the cellular immune response by decreasing serum TNF-α and IL-1ß concentrations, which was associated with an anti-inflammatory effect.
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Infecções por Coronavirus , Lipossomos , Nanopartículas , Vírus da Diarreia Epidêmica Suína , Doenças dos Suínos , Vacinas Virais , Animais , Suínos , Camundongos , Anticorpos Antivirais , Vírus da Diarreia Epidêmica Suína/genética , Ácido Glicirrízico/farmacologia , Glicoproteína da Espícula de Coronavírus , Adjuvantes Imunológicos , Imunidade , Proteínas Recombinantes , LipídeosRESUMO
BACKGROUND: There are few studies that assess the relationship between nutritional status and quality of life in individuals with Parkinson's disease, despite the well-established negative impact of sarcopenia and dynapenia on functional capacity and quality of life. These conditions impair protein-muscular status and are prevalent in individuals with Parkinson's disease. This study aimed to examine the relationship between nutritional status, including body composition, functional capacity, and diagnosis of sarcopenia and dynapenia, and quality-of-life perception in individuals with Parkinson's disease. METHODS: This is a cross-sectional study conducted in two Parkinson's disease centers in the northeast of Brazil. The researchers assessed muscle strength, muscle mass, and physical performance to diagnose dynapenia, sarcopenia and functional capacity. Quality of life was estimated using the Parkinson's disease Quality of Life Questionnaire. RESULTS: We found positive correlations between quality of life and variables such as severity and duration of the disease, as well as positive screening for sarcopenia (p<0.001). Negative correlations were observed between quality of life and muscle strength and functional capacity. The study also found that individuals with sarcopenia and dynapenia had significantly worse quality-of-life scores compared to those who did not have these nutritional outcomes (p <0.05; p <0.001). CONCLUSIONS: The presence of sarcopenia, dynapenia, low gait speed, disease duration, and severity had an impact on higher scores in the Parkinson's Disease Quality of Life Questionnaire, indicating a worsening perception of quality of life.
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Doença de Parkinson , Sarcopenia , Pessoa de Meia-Idade , Humanos , Idoso , Sarcopenia/diagnóstico , Doença de Parkinson/complicações , Qualidade de Vida , Estudos Transversais , Percepção , Força da Mão/fisiologiaRESUMO
Porcine rubulavirus (PRV) is a contagious virus that affects the Mexican swine industry. This work aimed to evaluate the immunogenicity of an recombinant hemagglutinin neuraminidase-Porcine rubulavirus (rHN-PorPV) candidate vaccine on pregnant sows, and the protective efficacy afforded to their 7-day-old suckling piglets against PRV lethal challenge. Three sows were immunized with rHN-PorPV formulated with immune-stimulating complex (ISCOMs) and two sows with rHN-PorPV protein alone as well as a mock-immunized pregnant sow (negative control). Quantitative ELISA detected a high concentration of anti-rHN-PorPV Immunoglobulin G (IgG) antibodies in sow sera after the second dose of vaccine administered on day 14 until farrowing, showing viral-neutralizing and cross-neutralization activity against different variants of PRV. Sera samples from piglets of immunized sows (with or without adjuvant), showed high concentrations of IgG antibodies. As expected, piglets from the negative control sow (n=5), exhibited severe signs of disease and 100% of mortality after PRV challenge study. Conversely, 75% and 87.5% of the piglets born from the rHN-PorPV and the rHN-PorPV-ISCOMs-immunized sows (n=8), survived, respectively, showing milder PRV clinical signs. Our data indicate that rHN-PorPV candidate vaccine produced in Escherichia coli induces efficient humoral response in pregnant sows and that the maternally derived immunity provides high protection to suckling piglets against PRV lethal challenge.
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Infecções por Escherichia coli , ISCOMs , Doenças dos Suínos , Gravidez , Animais , Suínos , Feminino , Neuraminidase/genética , Hemaglutininas , Escherichia coli/genética , Anticorpos Antivirais , Proteínas Virais , Infecções por Escherichia coli/veterinária , Imunoglobulina G , ColostroRESUMO
Blue eye disease (BED) is a swine viral infection that affects the pork industry of Mexico. Porcine orthorubulavirus (PRV) is the etiological agent, and the hemagglutinin-neuraminidase protein (HN) is characterized as the best antigen for serological tests, although other structural proteins, including the nucleoprotein (NP) and the matrix (M) protein, have been investigated during the infection of members of the Paramyxoviridae family, generating promising results. Herein, for the first time, we successfully produced and characterized both the NP and M proteins of PRV by using a recombinant strategy in the E. coli heterologous system. The ORF of the NP and M genes were cloned in-frame with the pET-SUMO expression vector. Recombinant proteins proved to be a sensitive target to detect seroconversion at 7 days until 28 days in vaccinated mice (BALB/c) by indirect ELISAs. Immunoreactivity was also tested using porcine serum samples, in which antibodies were recognized from early stages to a persistence of PRV infection, which is indicative that these proteins contain properties similar to native antigens. The predicted tertiary structure showed that both proteins have a conserved structure that resembles those found in others Paramyxovirus. Our results pave the way for developing biotechnological tools based on these proteins for the control and prevention of BED.
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Neuraminidase , Nucleoproteínas , Animais , Anticorpos Antivirais , Antígenos Virais/genética , Escherichia coli/genética , Hemaglutininas , Camundongos , Nucleoproteínas/genética , Proteínas Recombinantes/genética , Rubulavirus , SuínosRESUMO
La ecografía de pie de cama se ha abierto paso en diferentes escenarios como método para disminuir la incertidumbre diagnóstica. Existen ya numerosas publicaciones que dan cuenta que el uso de ecografía aumenta la capacidad resolutiva del primer nivel de atención. Este reporte de caso intenta poner en evidencia como la ecografía accesible en el primer nivel puede resolver un proceso de moderada complejidad diagnóstica, evitando demoras, y así también mayores complicaciones (AU)
Bedside ultrasonography has made its way into different settings as a method to reduce diagnostic uncertainty. There are already numerous publications that show that the use of ultrasonography increa-ses the resolution capacity of the first level of care. This case report tries to show how accessible ultrasound at the first level can solve a process of moderate diagnostic complexity, avoiding delays, and thus also greater complications (AU)
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Humanos , Masculino , Adulto , Atenção Primária à Saúde , Ultrassonografia , Trombose Venosa Profunda de Membros Superiores/diagnóstico por imagemRESUMO
INTRODUCCIÓN: Se describe la población usuaria del implante subdérmico (ISD) colocado por el equipo de salud, entre junio de 2016 a junio del 2019 en el marco del Programa de Salud Sexual y Reproductiva y Procreación Responsable. OBJETIVOS: Describir la población incluida en el programa, usuarias del ISD, que se lo colocaron en la US de Loma Paraguaya entre 1/6/2016 al 1/6/2019. MATERIALES Y MÉTODOS: Estudio Observacional-Transversal-Descriptivo. Mujeres en edad fértil que se hayan colocado un ISD en la US de Loma Paraguaya entre 1/6/2016 al 1/6/2019. RESULTADOS: El total de usuarias fue 52 mujeres registradas. La media de edad: 24 años, con un rango entre 14-41 años. El 55.7% (29) de las usuarias tenía al momento de la colocación entre 15 y 24 años (rango propuesto por el PSSyPR), 1 usuaria (1.9%) tenía menos de 15 años y 42,3% (22 pacientes) tenía una edad superior a los 24 años. DISCUSIÓN: La incorporación del ISD como MAC permitió aportar una opción más como método de larga duración, con el objetivo de disminuir el número de embarazos no intencionales en adolescentes y su repitencia. Más de la mitad de la población usuaria del ISD se encontraba dentro del rango etario; sin embargo, un 42% de las usuarias son mayores de 24 años. CONCLUSIONES: En nuestra población el ISD es solicitado por un grupo etario amplio de 14 a 41 años, la media es de 24 años. Ampliar el criterio de recomendación para su uso lo pone al alcance de las mujeres y sus preferencias (AU)
INTRODUCTION: The population using the subdermal implant (ISD) placed by the health team is described between June 2016 to June 2019 within the framework of the Sexual and Reproductive Health and Responsible Procreation Program. OBJECTIVES: To describe the population included in the program, users of the ISD, who were placed in the US of Loma Paraguaya between 1/6/2016 to 1/6/2019. MATERIALS AND METHODS: Observational-Cross-Sectional-Descriptive Study. Women of childbearing age who have had an ISD in the US of Loma Paraguaya between 1/6/2016 to 1/6/2019. RESULTS: The total of users was 52 registered women. The average age: 24 years, with a range between 14-41 years. 55.7% (29) of the users had at the time of placement between 15 and 24 years (range proposed by the PSSyPR), 1 user (1.9%) was under 15 years old and 42.3% (22 patients) had a age over 24 years. DISCUSSION: the incorporation of ISD as MAC allowed us to provide one more option as a long-term method, with the aim of reducing the number of unintended pregnancies in adolescents and their repetition. More than half of the ISD user population was within the age range; however, 42% of users are over 24 years old. CONCLUSIONS: In our population, ISD is requested by a broad age group of 14 to 41 years, the average is 24 years. Expanding the recommendation criteria for its use makes it available to women and their preferences (AU)
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Humanos , Feminino , Gravidez , Centros de Saúde , Anticoncepção/métodos , Implantes de Medicamento , Argentina , Serviços de Saúde ReprodutivaRESUMO
In Mexico, the first outbreaks suggestive of the circulation of the porcine epidemic diarrhea virus (PEDV) were identified at the beginning of July 2013. To identify the molecular characteristics of the PEDV Spike (S) gene in Mexico, 116 samples of the intestine and diarrhea of piglets with clinical signs of porcine epidemic diarrhea (PED) were obtained. Samples were collected from 14 farms located in six states of Mexico (Jalisco, Puebla, Sonora, Veracruz, Guanajuato, and Michoacán) from 2013 to 2016. To identify PEDV, we used real-time RT-PCR to discriminate between non-INDEL and INDEL strains. We chose samples according to state and year to characterize the S gene. After amplification of the S gene, the obtained products were sequenced and assembled. The complete amino acid sequences of the spike protein were used to perform an epitope analysis, which was used to determine null mutations in regions SS2, SS6, and 2C10 compared to the sequences of G2. A phylogenetic analysis determined the circulation of G2b and INDEL strains in Mexico. However, several mutations were recorded in the collagenase equivalent (COE) region that were related to the change in polarity and charge of the amino acid residues. The PEDV strain circulating in Jalisco in 2016 has an insertion of three amino acids (232LGL234) and one change in the antigenic site of the COE region, and strains from the years 2015 and 2016 changed the index of the surface probability, which could be related to the re-emergence of disease outbreaks.
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Infecções por Coronavirus/veterinária , Variação Genética , Vírus da Diarreia Epidêmica Suína/classificação , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , Glicoproteína da Espícula de Coronavírus/genética , Doenças dos Suínos/virologia , Animais , Análise por Conglomerados , Colagenases/genética , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , Epitopos/genética , Fezes/virologia , Intestinos/virologia , México/epidemiologia , Epidemiologia Molecular , Mutação , Filogenia , Vírus da Diarreia Epidêmica Suína/genética , Reação em Cadeia da Polimerase em Tempo Real , Análise de Sequência de DNA , Homologia de Sequência , Suínos , Doenças dos Suínos/epidemiologiaRESUMO
BACKGROUND: Genes encoding the receptors involved in the dopaminergic and serotonergic pathways are potential candidates in the mechanisms of nicotine addiction. AIMS: To identify genetic variants in the promoter regions and exons of the DRD4 and HTR2A genes associated with tobacco smoking and the degree of nicotine addiction in Mexican mestizos. METHODS: The study included 438 non-smokers (NS) and 1,157 current smokers, ranked based on their consumption of cigarettes per day (cpd): 574 heavy smokers (HS, >20 cpd) and 583 light smokers (LS, 1-10 cpd). Genotyping was performed for 4 and 8 single nucleotide polymorphisms (SNPs) in the DRD4 and HTR2A genes, respectively. RESULTS: The C allele of rs1800955 in DRD4 was found to be associated with cigarette smoking in the HS vs. NS and LS vs. NS comparisons (p = 2.34E-03 and p = 1.13E-03, respectively); the association was maintained in the homozygous CC genotype (p = 5.00E-04 and p = 2.00E-04, respectively). The T allele of rs6313 in HTR2A was significantly associated with cigarette smoking and a greater degree of nicotine addiction (p = 4.77E-03, OR = 1.55); the association was maintained in the homozygous genotype (TT) (p = 4.90E-03, OR = 1.96). The A allele of rs6313 was associated with cigarette smoking in the HS vs. NS comparison (p = 1.53E-02, OR = 1.36); the risk was nearly doubled in the homozygous AA genotype (p = 1.30E-03, OR = 1.83) compared with the heterozygous GA genotype (OR = 1.38). CONCLUSIONS: Among Mexican mestizos, the C allele of rs1800955 in the DRD4 gene and the A allele of rs6311 in the HTR2A gene are associated with cigarette smoking, whereas the T allele of rs6313 in HTR2A is associated with cigarette smoking and the degree of nicotine addiction.