RESUMO
BACKGROUND AND AIMS: An increase in plasma branched-chain amino acids is associated with a higher risk of developing type 2 diabetes and cardiovascular diseases. However, little is known about the basal plasma amino acid concentrations in young adults. Our aim was to determine the plasma amino acid profiles of young adults and to evaluate how these profiles were modified by sex, body mass index (BMI) and insulin resistance (IR). METHODS AND RESULTS: We performed a transversal study with 608 Mexican young adults aged 19.9 ± 2.4 years who were applicants to the Universidad Autónoma de San Luis Potosí. The subjects underwent a physical examination and provided a clinical history and a blood sample for biochemical, hormonal and amino acid analyses. The women had higher levels of arginine, aspartate and serine and lower levels of α-aminoadipic acid, cysteine, isoleucine, leucine, methionine, proline, tryptophan, tyrosine, urea and valine than the men. The obese subjects had higher levels of alanine, aspartate, cysteine, ornithine, phenylalanine, proline and tyrosine and lower levels of glycine, ornithine and serine than the normal weight subjects. Subjects with IR (defined as HOMA > 2.5) had higher levels of arginine, alanine, aspartate, isoleucine, leucine, phenylalanine, proline, tyrosine, taurine and valine than the subjects without IR. Furthermore, we identified two main groups in the subjects with obesity and/or IR; one group was composed of amino acids that positively correlated with the clinical, biochemical and hormonal parameters, whereas the second group exhibited negative correlations. CONCLUSION: This study demonstrates that young adults with obesity or IR have altered amino acid profiles characterized by an increase in alanine, aspartate, proline and tyrosine and a decrease in glycine.
Assuntos
Aminoácidos/sangue , Índice de Massa Corporal , Resistência à Insulina , Obesidade Infantil/sangue , Adolescente , Fenômenos Fisiológicos da Nutrição do Adolescente , Fatores Etários , Biomarcadores/sangue , Estudos Transversais , Feminino , Humanos , Masculino , México/epidemiologia , Estado Nutricional , Obesidade Infantil/diagnóstico , Obesidade Infantil/epidemiologia , Obesidade Infantil/fisiopatologia , Prevalência , Fatores de Risco , Fatores Sexuais , Adulto JovemRESUMO
The aim of the present study was to evaluate associations between intestinal parasitic infection with intestinal and systemic inflammatory markers in school-aged children with high rates of obesity. Plasma concentrations of C-Reactive Protein (CRP), leptin, TNF-α, IL-6 and IL-10 were measured as systemic inflammation markers and count of stool leukocytes as marker of intestinal inflammation in 291 children (6-10 years). Intestinal parasitic infection was measured by stool examination. Logistic regression analyses were performed to determine the odds of having high inflammatory markers for each parasite or group of parasites as compared to parasite-free children while adjusting for sex, age, mother's educational level and percentage of body fat. The prevalence of soil-transmitted helminths and intestinal protozoa infections was 12% and 36%, respectively. Parasitic infection was not associated with CRP, IL-6, IL-10 or TNF-α. Children infected with Ascaris lumbricoides (aOR: 5.91, 95% CI: 1.97-17.70) and Entamoeba coli (aOR: 8.46, 95% CI: 2.85-25.14) were more likely to have higher stool leucocytes than parasite-free children. Children with multiple infections (aOR: 10.60, 95% CI: 2.85-25.14) were more likely to have higher leptin concentrations than parasite-free children. Intestinal parasitic infection was not associated with systemic inflammation, but was associated with intestinal inflammation. Having multiple infections were associated with higher leptin concentrations.
Assuntos
Helmintíase/sangue , Helmintíase/imunologia , Enteropatias Parasitárias/sangue , Enteropatias Parasitárias/imunologia , Intestinos/parasitologia , Leptina/sangue , Animais , Ascaris lumbricoides/imunologia , Proteína C-Reativa/metabolismo , Criança , Estudos Transversais , Entamoeba histolytica/imunologia , Fezes/parasitologia , Feminino , Helmintíase/parasitologia , Humanos , Inflamação , Interleucina-10/sangue , Interleucina-6/sangue , Enteropatias Parasitárias/parasitologia , Masculino , Obesidade/complicações , Prevalência , Inquéritos e Questionários , Fator de Necrose Tumoral alfa/sangueRESUMO
BACKGROUND: Intestinal parasites, virus and bacterial infections are positively associated with obesity and adiposity in vitro and in animal models, but conclusive evidence of this relationship in humans is lacking. The aim of this cross-sectional study was to determine differences in adiposity between infected and non-infected children, with a high prevalence of intestinal parasitic infection and obesity. SUBJECTS: A total of 296 school-aged children (8.0 ± 1.5 years) from a rural area in Querétaro, Mexico, participated in this study. Anthropometry (weight, height and waist circumference) and body fat (DXA) were measured in all children. A fresh stool sample was collected from each child and analysed for parasites. Questionnaires related to socioeconomic status and clinical history were completed by caretakers. RESULTS: Approximately 11% of the children were obese, and 19% were overweight. The overall prevalence of infection was 61%. Ascaris lumbricoides was the most prevalent soil transmitted helminth (16%) followed by hookworm. Entamoeba coli was the predominant protozoa (20%) followed by Endolimax nana, Balantidium coli, Entamoeba histolytica/dispar, Iodamoeba bütschlii and Giardia lamblia. Children with moderate-heavy infection of E. coli had significantly higher waist circumference, waist-to-height ratio, body and abdominal fat than children not infected or with light-intensity infection (p < 0.05). CONCLUSION: These findings raise the possibility that a moderate or heavy infection with E. coli may contribute to fat deposition and thereby have long-term consequences on human health. Further studies are needed to better understand if E. coli contributes directly to fat deposition and possible mechanisms.
Assuntos
Adiposidade , Entamoeba/isolamento & purificação , Entamebíase/fisiopatologia , Enteropatias Parasitárias/fisiopatologia , Obesidade Infantil/fisiopatologia , Gordura Abdominal , Adiposidade/fisiologia , Antropometria , Criança , Estudos Transversais , Entamebíase/parasitologia , Fezes/parasitologia , Feminino , Humanos , Enteropatias Parasitárias/parasitologia , Masculino , México , Obesidade Infantil/parasitologia , Prevalência , Classe SocialRESUMO
ABSTRACT The aim of the present study was to verify the occurrence of ants in 5 hospitals in the city of Pelotas, analyzing their seasonality and identifying associated pathogenic bacteria. For this, 2 baits were used, one containing honey, sugar and the earthworm Eisenia foetida. and another containing Coqueiro® brand sardines. Both baits were fixed close to the floor skirtings in 19 to 21 sectors in each hospital (99 sectors in total), and collected after 24h. The study was developed over the seasons of 2008 and 2009, for a total of 792 sampling points. A total of 7 ant species were found, distributed among 13 of the 99 sectors sampled (13.1%). Summer was the season with the highest percentage of specimens gathered, 57.6%, with the lowest number in the winter, 9.1%. A total of 28 ant samples were aseptically collected, from which 25 (89.3%) showed the occurrence of pathogenic bacteria. Enterobacteriaceae were found on 17 ant samples, followed by Staphylococcus epidermidis and Staphylococcus saprophyticus in 13 and 9 samples, respectively. Furthermore, ants from 20 sampling points showed the occurrence of more than one bacteria species, the maximum being 5. It was concluded that the ants are important carriers of pathogenic bacteria in hospitals, and must be controlled in order to avoid a higher occurrence of nosocomial infections. Moreover, attention must be given to the ant management in the external areas of the hospitals, where the colonies of most of the specimens collected are located.
RESUMO O presente estudo teve por objetivo verificar a ocorrência de formigas em cinco hospitais do Município de Pelotas, analisar sua sazonalidade e identificar bactérias patogênicas a elas associadas. Para tal, foram utilizadas duas iscas, uma contendo mel, açúcar e minhoca da espécie Eisenia foetida, e outra contendo sardinha da marca Coqueiro®. Ambas foram fixadas próximas aos rodapés de 19 a 21 setores de cada hospital (99 setores no total), e retiradas após 24h. O trabalho foi realizado durante as quatro estações de 2008 e 2009, totalizando 792 pontos amostrais. Foram encontradas sete espécies de formigas, distribuídas em 13 setores dentre os 99 amostrados (13,1%). O verão foi a estação onde a maior porcentagem de amostras foi coletada, 57,6%, sendo o menor número no inverno, 9,1%. Foram coletadas assepticamente formigas de 28 pontos amostrais, sendo que 25 (89,3%) apresentaram a ocorrência de bactérias patogênicas. As Enterobactérias estiveram presentes em 17 amostras, seguidas por Staphylococcus epidermidis e Staphylococcus saprophyticus em 13 e 9 amostras, respectivamente. Além disso, formigas de 20 pontos amostrais apresentaram mais de uma espécie bacteriana, com um máximo de cinco. Concluiu-se que as formigas são importantes carreadoras de bactérias patogênicas nos hospitais do município, e devem ser controladas a fim de se evitar maior ocorrência de infecções nosocomiais. Deve ser dada atenção ao controle na área externa dos hospitais, onde ocorre a nidificação da maioria dos espécimes coletados.
RESUMO
O presente estudo teve por objetivo verificar a ocorrência de formigas em cinco hospitais do Município de Pelotas, analisar sua sazonalidade e identificar bactérias patogênicas a elas associadas. Para tal, foram utilizadas duas iscas, uma contendo mel, açúcar e minhoca da espécie Eisenia foetida, e outra contendo sardinha da marca Coqueiro®. Ambas foram fixadas próximas aos rodapés de 19 a 21 setores de cada hospital (99 setores no total), e retiradas após 24h. O trabalho foi realizado durante as quatro estações de 2008 e 2009, totalizando 792 pontos amostrais. Foram encontradas sete espécies de formigas, distribuídas em 13 setores dentre os 99 amostrados (13,1%). O verão foi a estação onde a maior porcentagem de amostras foi coletada, 57,6%, sendo o menor número no inverno, 9,1%. Foram coletadas assepticamente formigas de 28 pontos amostrais, sendo que 25 (89,3%) apresentaram a ocorrência de bactérias patogênicas. As Enterobactérias estiveram presentes em 17 amostras, seguidas por Staphylococcus epidermidis e Staphylococcus saprophyticus em 13 e 9 amostras, respectivamente. Além disso, formigas de 20 pontos amostrais apresentaram mais de uma espécie bacteriana, com um máximo de cinco. Concluiu-se que as formigas são importantes carreadoras de bactérias patogênicas nos hospitais do município, e devem ser controladas a fim de se evitar maior ocorrência de infecções nosocomiais. Deve ser dada atenção ao controle na área externa dos hospitais, onde ocorre a nidificação da maioria dos espécimes coletados.
The aim of the present study was to verify the occurrence of ants in 5 hospitals in the city of Pelotas, analyzing their seasonality and identifying associated pathogenic bacteria. For this, 2 baits were used, one containing honey, sugar and the earthworm Eisenia foetida. and another containing Coqueiro® brand sardines. Both baits were fixed close to the floor skirtings in 19 to 21 sectors in each hospital (99 sectors in total), and collected after 24h. The study was developed over the seasons of 2008 and 2009, for a total of 792 sampling points. A total of 7 ant species were found, distributed among 13 of the 99 sectors sampled (13.1%). Summer was the season with the highest percentage of specimens gathered, 57.6%, with the lowest number in the winter, 9.1%. A total of 28 ant samples were aseptically collected, from which 25 (89.3%) showed the occurrence of pathogenic bacteria. Enterobacteriaceae were found on 17 ant samples, followed by Staphylococcus epidermidis and Staphylococcus saprophyticus in 13 and 9 samples, respectively. Furthermore, ants from 20 sampling points showed the occurrence of more than one bacteria species, the maximum being 5. It was concluded that the ants are important carriers of pathogenic bacteria in hospitals, and must be controlled in order to avoid a higher occurrence of nosocomial infections. Moreover, attention must be given to the ant management in the external areas of the hospitals, where the colonies of most of the specimens collected are located.
Assuntos
Formigas , Bactérias/patogenicidade , Saúde Pública , Infecção Hospitalar , HospitaisRESUMO
BACKGROUND: The efficacy of micronutrient supplementation on growth may be modified by specific gastrointestinal parasite infections. METHODS: We carried out a double-blind placebo-controlled trial to evaluate the effect of vitamin A and zinc supplementation on gastro-intestinal pathogen infections and growth among 584 infants in Mexico City. Children aged 5-15 months were assigned to receive either a vitamin A supplement every 2 months (20,000 IU of retinol for infants < or =; 1 year or 45,000 IU for infants >1 year), a daily supplement of 20 mg of zinc, a combined vitamin A-zinc supplement or a placebo, and were followed up for 1 year. Weight and length were measured once a month and morbidity histories were recorded twice a week for 12 months. Monthly stool samples were screened for Giardia duodenalis, Ascaris lumbricoides and Entamoeba spp. Growth velocity slopes, generated from the linear regression of individual child length, and height-for-age z-scores on time were analyzed as end points in regression models, adjusting for the presence of parasite infections. RESULTS: The main effect of vitamin A supplementation was in height improvement (P<0.05), and was only found in the model evaluating infants with any parasite. There was an interaction effect of slower growth (P<0.05) found in infants infected with any parasite and supplemented with vitamin A in slower growth (P<0.05). In addition, the interaction of zinc supplementation and Giardia duodenalis or A. lumbricoides was associated with reduced growth (P<0.05). CONCLUSION: Gastro-intestinal parasite infections may modify the effect that zinc or vitamin A supplementation has on childhood growth.
Assuntos
Estatura/efeitos dos fármacos , Peso Corporal/efeitos dos fármacos , Crescimento/efeitos dos fármacos , Enteropatias Parasitárias/complicações , Vitamina A/farmacologia , Zinco/farmacologia , Animais , Ascaríase/complicações , Ascaris lumbricoides , Estatura/fisiologia , Peso Corporal/fisiologia , Suplementos Nutricionais , Disenteria Amebiana/complicações , Fezes/parasitologia , Feminino , Giardíase/complicações , Crescimento/fisiologia , Transtornos do Crescimento/etiologia , Transtornos do Crescimento/prevenção & controle , Humanos , Lactente , Masculino , México , Oligoelementos/farmacologia , Vitaminas/farmacologiaRESUMO
The implications of delta-aminolevulinic acid dehydratase (ALAD) polymorphism for lead kinetics and toxicity have been mainly studied in occupationally exposed adults. Therefore, our purpose was to evaluate the distribution of ALAD genotype and its association with biomarkers of exposure (PbB levels) and effect (Blood ZPP) among children living in a smelter community in Mexico. We recruited 569 children from nine elementary schools close to a smelter site. PbB was determined by electrothermal atomic absorption spectrometry. A polymerase chain reaction (PCR)-based protocol was used for ALAD genotyping. Zinc protoporphyrin (ZPP) in blood was measured by direct fluorometry. Most children (93.15%) were homozygous for ALAD (1-1), 6.67% were heterozygous for ALAD for (1-2), and one child was homozygous for ALAD (2-2). There was an increased proportion of ALAD (1-2/2-2) genotype with respect to PbB levels. The ZPP geometric mean was slightly higher in ALAD (1-1) genotype children (63.48 mu mol ZPP/mol Hb) than in those having the ALAD-2 genotype (58.22 mu mol ZPP/mol Hb; p = 0.051). Linear and quadratic models showed significant relationships between ZPP and PbB. A significant increase in the odds ratio (OR) for the effect of lead exposure on ZPP levels was observed for ALAD (1-1) children having PbB values above 20 mu g/dL, as compared to those having PbB levels below 10 mu g/dL (OR = 2.95, 95% CI = 1.45-5.97; p = 0.003), whereas no significant increases were observed for the ALAD (1-2/2-2) children. In summary, our results suggest that heme biosynthesis was less affected in ALAD (1-2/2-2) lead-exposed children than in those carrying the ALAD (1-1) genotype.
RESUMO
BACKGROUND: The role of single micronutrient deficiencies in the etiology of growth retardation has recently gained attention. However, because multiple micronutrient deficiencies are common in children in developing countries, it is possible that more than one micronutrient may limit growth and, hence, the correction of a single deficiency may not be enough to improve growth substantially. OBJECTIVE: The objective was to evaluate the effect of multiple micronutrient supplementation on the growth of children aged 8-14 mo whose diets were poor in several micronutrients. DESIGN: Children were randomly assigned to 1 of 2 groups. One group received a multiple micronutrient supplement containing the recommended dietary allowance (RDA) or 1.5 times the RDA of vitamins A, D, E, K, C, B-1, B-6, B-12, riboflavin, niacin, biotin, folic acid, and pantothenic acid, and iron, zinc, iodine, copper, manganese, and selenium. The other group received a placebo. Supplements were administered 6 d/wk for an average of 12.2 mo. Body length was measured at baseline and monthly thereafter until the end of supplementation. RESULTS: Supplemented infants initially aged <12 mo had significantly greater length gains than did the placebo group, with a difference of 8.2 mm (length-for-age z score: 0.3) at the end of supplementation. In contrast, differences in length gains between the supplemented and placebo groups initially aged > or =12 mo were not significant. CONCLUSIONS: Micronutrient deficiencies limited the growth of the Mexican infants studied. Improving micronutrient intakes should be a component of interventions to promote growth in infants living in settings where micronutrient intakes are inadequate.
Assuntos
Estatura/efeitos dos fármacos , Suplementos Nutricionais , Transtornos do Crescimento/etiologia , Crescimento/efeitos dos fármacos , Micronutrientes/administração & dosagem , Fatores Etários , Estatura/fisiologia , Desenvolvimento Infantil , Método Duplo-Cego , Feminino , Crescimento/fisiologia , Transtornos do Crescimento/epidemiologia , Transtornos do Crescimento/prevenção & controle , Humanos , Lactente , Masculino , México , Micronutrientes/farmacologiaRESUMO
BACKGROUND: In developing countries, incomplete resolution of anemia with iron supplementation is often attributed to poor compliance or inadequate duration of supplementation, but it could result from deficiencies of other micronutrients. OBJECTIVE: Our objective was to assess children's hematologic response to supervised, long-term iron supplementation and the relation of this response to other micronutrient deficiencies, anthropometry, morbidity, and usual dietary intake. DESIGN: Rural Mexican children aged 18-36 mo (n = 219) were supplemented for 12 mo with either 20 mg Fe, 20 mg Zn, both iron and zinc, or placebo. Children were categorized as iron-unsupplemented (IUS; n = 109) or iron supplemented (IS; n = 108). Hemoglobin, hematocrit, mean corpuscular volume, mean cell hemoglobin, plasma concentrations of micronutrients that can affect hematopoiesis, anthropometry, and diet were assessed at 0, 6, and 12 mo; morbidity was assessed biweekly. RESULTS: At baseline, 70% of children had low hemoglobin (
Assuntos
Anemia/tratamento farmacológico , Suplementos Nutricionais , Hemoglobinas/metabolismo , Ferro/administração & dosagem , Pré-Escolar , Dieta , Índices de Eritrócitos , Ferritinas/sangue , Ferritinas/deficiência , Hematócrito , Hematopoese , Humanos , Lactente , México , Estado Nutricional , População Rural , Deficiência de Vitamina A/sangue , Deficiência de Vitamina B 12/sangue , Deficiência de Vitamina E/sangueRESUMO
BACKGROUND: The coexistence of multiple micronutrient deficiencies is a widespread public health problem in many regions of the world. Interactions between zinc deficiency and vitamin A metabolism have been reported but no longitudinal studies have evaluated the effect of iron deficiency on vitamin A. OBJECTIVE: The objective of this study was to investigate the effect of supplementation with iron, zinc, or both on vitamin A and its metabolically related proteins retinol binding protein (RBP) and transthyretin. DESIGN: The study was a longitudinal, double-blind, placebo-controlled trial in which 219 rural Mexican children aged 18-36 mo were randomly assigned to receive 20 mg Zn/d, 20 mg Fe/d, 20 mg Zn/d plus 20 mg Fe/d, or placebo. RESULTS: Six months after supplementation, plasma retinol increased in all supplemented groups. Compared with placebo, zinc supplementation was associated with significantly higher plasma retinol and transthyretin but the increase in RBP was not significant. Iron supplementation significantly increased plasma retinol, RBP, and transthyretin. Supplementation with zinc plus iron significantly increased plasma retinol but not RBP or transthyretin. Children deficient in zinc, iron, or vitamin A (as indicated by nutrient plasma concentration) at the beginning of the study had a significantly greater increase in retinol than did children with adequate nutrient status. CONCLUSIONS: Supplementation with zinc, iron, or both improved indicators of vitamin A status. The results of this study agree with previous observations of a metabolic interaction between zinc and vitamin A and suggest an interaction between iron and vitamin A metabolism.