Assuntos
Anticorpos Antivirais/sangue , Doenças dos Cavalos/epidemiologia , Cavalos/virologia , Vírus da Influenza A/imunologia , Vírus do Nilo Ocidental/imunologia , Animais , Doenças dos Cavalos/virologia , Vírus da Influenza A/isolamento & purificação , México/epidemiologia , Infecções por Orthomyxoviridae/epidemiologia , Infecções por Orthomyxoviridae/veterinária , Estudos Soroepidemiológicos , Febre do Nilo Ocidental/epidemiologia , Febre do Nilo Ocidental/veterinária , Vírus do Nilo Ocidental/isolamento & purificaçãoRESUMO
The co-circulation of all 4 dengue virus serotypes in the same community, common since the 1950s in Southeast Asia, has now become a frequent occurrence in many Caribbean Islands, Mexico, and Central and South America in the past 20 years. As a consequence, the frequency of concurrent infections would be expected to increase in these areas. To assess this, using state of the art technology, we screened viremic serum samples and mosquitoes inoculated with serum samples collected during epidemics involving multiple dengue virus serotypes in Indonesia, Mexico, and Puerto Rico for virus isolation. Of 292 samples tested, 16 (5.5%) were found to contain 2 or more dengue viruses by an indirect immunofluorescence test and/or the reverse transcriptase-polymerase chain reaction.
Assuntos
Vírus da Dengue/classificação , Surtos de Doenças , Dengue Grave/virologia , Animais , Anticorpos Monoclonais , Anticorpos Antivirais/sangue , Bioensaio , Células Cultivadas , Culicidae/virologia , Primers do DNA/química , Vírus da Dengue/genética , Vírus da Dengue/imunologia , Vírus da Dengue/patogenicidade , Eletroforese em Gel de Ágar , Técnica Direta de Fluorescência para Anticorpo , Humanos , Indonésia/epidemiologia , México/epidemiologia , Porto Rico/epidemiologia , RNA Viral/química , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sorotipagem , Dengue Grave/sangue , Dengue Grave/epidemiologiaRESUMO
An outbreak of dengue 4 occurred in the Yucatán, México in 1984. During the course of the outbreak, 538 of 5486 reported cases of dengue-like illness were studied; 200 were confirmed as dengue serologically and/or virologically. Dengue 4 virus was isolated from 34 patients and dengue 1 from one. Severe haemorrhagic symptoms were observed in 9 laboratory confirmed patients, including four deaths. Thus, the outbreak in Yucatán is the second dengue epidemic in the Americas after the Cuban epidemic in 1981 in which a number of patients suffered from haemorrhagic complications. It was notable that 5 of 9 hospitalized, severe cases were young adults and that only one met the WHO criteria of DHF, in contrast to primary pediatric nature of DHF in Southeast Asia. In this paper we describe clinical, serologic, and virologic studies conducted during the outbreak.