RESUMO
Cytogenetic and molecular analyses enabled identification of two cytotypes among individuals of the spotted scorpion fish Scorpaena plumieri from Margarita Island, Venezuela. Cytotype 1 was characterized by 48 subtelo-acrocentric chromosomes and fundamental number (number of chromosome arms; FN) equalled 48, while cytotype 2 was characterized by two metacentric and 46 subtelo-acrocentric chromosomes and FN was 50. These cytotypes also differed in the location of the ribosomal gene clusters and in the distribution of the constitutive heterochromatin. Moreover, fish from the cytotypes 1 and 2 were found to belong to distinct mitochondrial lineages. The presence of two S. plumieri cytotypes from two lineages separated by high genetic distance suggests that they correspond to sympatric cryptic species.
Assuntos
Citogenética , Perciformes/classificação , Perciformes/genética , Animais , Região do Caribe , Heterocromatina , Hibridização in Situ Fluorescente , Família Multigênica/genética , Especificidade da Espécie , VenezuelaRESUMO
Unisexual poeciliid fishes collected from two localities in the Soto la Marina drainage have been analyzed. Unisexual diploid and triploid specimens with anomalous karyotypes were found among karyotypically normal specimens. Chromosomal data are discussed in light of the origin of clonal diversity in unisexual/bisexual breeding complexes and with regard to the role of triploids in these breeding complexes.
Assuntos
Peixes/genética , Sexo , Animais , Bandeamento Cromossômico , Feminino , Cariotipagem , México , Região Organizadora do Nucléolo/ultraestruturaRESUMO
Chromosomes of Poecilia mexicana mexicana, one of the bisexual species involved in the hybrid origin of the unisexual teleost fish species P. formosa, were analyzed by several staining techniques. Sex-specific, differential heterochromatin, found in other congeneric species, was not observed in P. m. mexicana. Nucleolar organizer regions were polymorphic among individual specimens within a given population sample. A single specimen exhibiting intraindividual variability of chromosome pair 1 and a specimen with a triploid karyotype are also described.
Assuntos
Bandeamento Cromossômico , Heterocromatina , Região Organizadora do Nucléolo , Poecilia/genética , Coloração e Rotulagem , Animais , Cromomicina A3 , Feminino , Corantes Fluorescentes , Indóis , Masculino , Quinacrina , Coloração pela PrataRESUMO
Os autores relatam o quadro de um paciente de 21 anos, portador de doenca de Refsum, com retinose pigmentar atipica, hemeralopia, hipoacusia neurosensorial bilateral, ataxia, polineuropatia periferica e estrabismo divergente a direita. Enfatizam a incidencia na raca negra e comentam as alteracoes gastrointestinais encontradas
Assuntos
Adulto , Humanos , Masculino , Doença de RefsumAssuntos
Adulto , Pessoa de Meia-Idade , Humanos , Masculino , Exercício Físico , Ureia , Ácido ÚricoRESUMO
The authors showed that the colorimetric methods used to effect aminoacidopathies screening are inadequate and unspecific. The thin layer chromatography was chosen to effect such screening and the authors showed the incidence in newborns of some aminoacid metabolism abnormalities.
Assuntos
Doenças do Recém-Nascido/diagnóstico , Aminoacidúrias Renais/diagnóstico , Erros Inatos do Metabolismo dos Aminoácidos/diagnóstico , Aminoácidos/urina , Cromatografia em Camada Fina/métodos , Colorimetria/métodos , Humanos , Recém-Nascido , Programas de RastreamentoRESUMO
The authors observed the influence of the B1 aflatoxin over rat liver and kidneys glucose-6-phosphatase activity. Animals aged 30 and 60 days received B1 aflatoxin in oil, 1 mcg/g and 5 mcg/g of body weight. The parameters were observed 6, 24 and 48 hours after the micotoxin administration. There was significant decrease of glucemia in the 30 days animals. In the 60 days animals the results suggested possible increase in the liver glucose-6-phosphatase and marked decrease in glycogen.