RESUMO
OBJECTIVE: To assess trabecular bone structure as calculated with fractal analysis by 2 binarization processes: White and Rudolph's original method (WR.o) and a revised version (WR.r). Fractal dimension (FD) values calculated with WR.r (FD.r) and a gray-scale-based method (FD.f) were also compared. FD, histogram parameters, and lacunarity were compared by dentate status, jaw location, and sex. STUDY DESIGN: Regions of interest from digital periapical radiographs were defined below the teeth roots and in the edentulous sites of 37 patients. Histograms were assessed for pixel values. Binarization was performed with WR.o and then with WR.r, in which the outliers were removed. FD was assessed using WR.r (FD.r) and (FD.f). Histograms were assessed to obtain pixel values. Lacunarity was calculated. RESULTS: WR.r revealed fewer trabeculae, branches, and junctions than WR.o (P < .0001). The majority of the mean differences between FD.r and FD.f were within the 95% CI. Dentate areas had greater mean gray levels than partially edentulous areas (P = .0027). FD.f was higher in the mandible (P = .01), but gray-level SD (P < .0001) and lacunarity (P = .02) were greater in the maxilla. FD.f and lacunarity were higher (P = .0005) and lower (P = .0014) in males, respectively. CONCLUSION: WR.r was effective in revealing skeletonized bone trabeculae by removing non-trabecular noise. FD.r and FD.f revealed good agreement. FD.f, histogram parameters, and lacunarity differed based on dentate status, jaw location, and sex.
Assuntos
Fractais , Boca Edêntula , Masculino , Humanos , Processamento de Imagem Assistida por Computador/métodos , Mandíbula/diagnóstico por imagem , Maxila/diagnóstico por imagemRESUMO
PURPOSE: Zoledronic acid (ZOL) and denosumab (Dmab) are commonly used to treat bone pathologies. Because these drugs suppress bone metabolism, this study sought to compare their effect on bone repair after tooth extraction. MATERIALS AND METHODS: Four-week-old male Wistar rats were randomly assigned to 1 of 3 groups: ZOL 0.125 mg/kg, Dmab 0.25 mg/kg, or saline solution 10 mL/kg (control). After 1 week of treatment, the first left molar was extracted; the rats were euthanized at 28 days. The jaws were removed and photographed for macroscopic analysis of wound healing and then subjected to tomographic and histologic analyses. Immunohistochemistry was carried out against the receptor activator of nuclear factor-κB ligand (RANKL) and osteoprotegerin (OPG). RESULTS: No difference in wound healing, presence of inflammatory infiltrate and bone sequestration, or osteocyte expression of RANKL and OPG was found among groups. Tomographic analysis showed that the ZOL group had less alveolar resorption and more complete alveolar repair compared with the other groups. There was a statistically significant difference in the OPG marker in the control (P = .008) and ZOL (P = .05) groups when comparing the extracted and non-extracted sides. CONCLUSION: Systemic use of ZOL can improve alveolar bone healing; however, the potential risk for the development of osteonecrosis should be considered. Higher expression of OPG seems to be associated with the control of osteoclastogenesis during bone repair.