RESUMO
BACKGROUND: Transmission dynamics of Cyclospora cayetanensis in endemic areas and the factors associated with soil contamination remain unclear. The effects of environmental factors on Cyclospora have been insufficiently studied, particularly in South America, thus a Venezuelan community was studied to profile risk factors for infection. METHODS: A cross-sectional stool survey of 732 individuals was conducted. For Cyclospora screening, an acid-fast-stained smear of formalin-ethyl acetate concentrate and ultraviolet (UV) epifluorescence examination of a wet mount were used. Water (n=14), soil (n=50) and produce (n=77) samples were collected, processed and examined by UV epifluorescence. Data were analysed using multivariate logistic regression. RESULTS: Cyclospora infections were identified in 73 (9.9%) subjects. Variables associated with the infection were age ≤10 y (odds ratio [OR] 14), hut living (OR 5), well water use (OR 18.5), drinking untreated water (OR 7.6), toilet absence (OR 8), having contact with faeces-contaminated soil (OR 4) and poultry exposure (OR 3). Infections (63%) were clustered in 25 huts. Oocysts were identified in 28.6%, 18% and 3.9% of the water, soil and produce samples, respectively. CONCLUSIONS: There was an explicit association of Cyclospora infection with extreme poverty and soil transmission reflecting the household socio-economic correlate of cyclosporiasis in this community.
Assuntos
Cyclospora , Ciclosporíase , Humanos , Ciclosporíase/epidemiologia , Ciclosporíase/diagnóstico , Solo , Estudos Transversais , Pobreza , ÁguaRESUMO
Few investigations have been conducted on risk factors for Cryptosporidium infection in communities from developing countries. A study was conducted to determine the prevalence and risk factors for cryptosporidiosis in San Carlos island, Venezuela. A sample of 515 subjects (mean age +/- SD: 21.4 +/- 17.8 years) was surveyed. Single fecal specimens were collected and modified Ziehl-Neelsen carbolfuchsin staining of formalin-ether concentrate stools were examined for identification of the parasite. Infections with Cryptosporidium (67 of 515, 13%) were common. Prevalence of the parasite varied among sectors of the community; 34 of 67(50.7%) cases of cryptosporidiosis clustered in two sectors with extreme poverty. Variables strongly associated with a higher risk for the infection (p < 0.01) were residing in these sectors versus the remainder, living in a hut or small residence versus a brick or larger house, using an area of backyard rather than a toilet or latrine for defecation, and having contact with soil contaminated with human feces. Crowding was also a risk (p < 0.05). Contact with human feces contaminated-soil may be an important mode of transmission and poverty a predisposing factor for the infection.
Assuntos
Criptosporidiose/epidemiologia , Cryptosporidium/isolamento & purificação , Diarreia/parasitologia , Fezes/parasitologia , Saneamento , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , Pré-Escolar , Estudos Transversais , Criptosporidiose/diagnóstico , Criptosporidiose/transmissão , Diarreia/epidemiologia , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Pobreza , Prevalência , Fatores de Risco , Venezuela/epidemiologiaRESUMO
Few investigations have been conducted on risk factors for Cryptosporidium infection in communities from developing countries. A study was conducted to determine the prevalence and risk factors for cryptosporidiosis in San Carlos island, Venezuela. A sample of 515 subjects (mean age ± SD: 21.4 ± 17.8 years) was surveyed. Single fecal specimens were collected and modified Ziehl-Neelsen carbolfuchsin staining of formalin-ether concentrate stools were examined for identification of the parasite. Infections with Cryptosporidium (67 of 515, 13 percent) were common. Prevalence of the parasite varied among sectors of the community; 34 of 67(50.7 percent) cases of cryptosporidiosis clustered in two sectors with extreme poverty. Variables strongly associated with a higher risk for the infection (p < 0.01) were residing in these sectors versus the remainder, living in a hut or small residence versus a brick or larger house, using an area of backyard rather than a toilet or latrine for defecation, and having contact with soil contaminated with human feces. Crowding was also a risk (p < 0.05). Contact with human feces contaminated-soil may be an important mode of transmission and poverty a predisposing factor for the infection.
Assuntos
Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Criptosporidiose/epidemiologia , Cryptosporidium/isolamento & purificação , Diarreia/parasitologia , Fezes/parasitologia , Saneamento , Estudos Transversais , Criptosporidiose/diagnóstico , Criptosporidiose/transmissão , Diarreia/epidemiologia , Pobreza , Prevalência , Fatores de Risco , Venezuela/epidemiologiaRESUMO
The epidemiology of Cyclospora cayetanensis is not well understood. Few community-based studies have addressed this issue. A study was conducted to determine the prevalence and risk factors for cyclosporiasis in San Carlos Island, Venezuela. A sample of 515 subjects (mean+/-SD: 21.4+/-17.8 years) was surveyed. For identification of the parasite, stools were examined with modified Ziehl-Neelsen carbolfuchsin staining of formalin-ether concentrates. Infections with Cyclospora (43 of 515, 8.3%) were common. There were differences in prevalence of the parasite among sectors of the community: 30 out of 43 (69.8%) cases of cyclosporiasis clustered in two sectors with extreme poverty. Living in these sectors versus the remainder, living in a hut or small residence versus a concrete or larger house, using an area of backyard rather than a toilet or latrine for defecation, and having contact with soil contaminated with human feces were factors strongly associated with the infection (P<0.01). Contact with soil contaminated with human feces might be an important mode of transmission, and poverty a predisposing factor, for the infection.
Assuntos
Ciclosporíase/epidemiologia , Diarreia/epidemiologia , Fezes/parasitologia , Adolescente , Adulto , Animais , Cyclospora/isolamento & purificação , Ciclosporíase/economia , Ciclosporíase/transmissão , Diarreia/economia , Diarreia/parasitologia , Feminino , Humanos , Masculino , Prevalência , Fatores de Risco , Fatores Socioeconômicos , Venezuela/epidemiologiaRESUMO
Se realizó un estudio parasitológico de Entamoeba histolytica y otros parásitos intestinales en 767 individuos de una comunidad de bajas condiciones socioeconómicas del Distrito Miranda, Estado Zulia. Se examinó un espécimen fecal por persona mediante frotis fecales teñidos según la técnica de la hematoxilina férrica-ácido fosfotúngstico de Tompkins y Miller y concentración de acuerdo al método del formol-éter de Ritchie