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Artigo em Inglês | MEDLINE | ID: mdl-20390843

RESUMO

We determined the hepatic Cytochrome P4501A (CYP1A) mRNA and Ethoxyresorufin-O-deethylase (EROD) activities in the fish, Ariopsis felis, from highly polluted to relatively pristine regions in the southwest Gulf of Mexico and their relationship with the genetic polymorphisms of the same fish. We hypothesized that a high genetic variation reflects interindividual variability in levels of CYP1A mRNA underlying the pathway culminating in EROD induction caused by the environmental contaminants. Catfish from Laguna de Mecoacan exhibited marked induction of CYP1A mRNA and high levels of hepatic EROD activities, whereas fish from Laguna de Celestun showed no induction of CYP1A mRNA and moderately low levels of EROD activities. In contrast, the similarity index considering all samples varied from 0.4 to 0.87, showing a wide range of variation. A dendrogram showed a clear grouping of fish collected from the Laguna de Terminos, Rio Coatzacoalcos and Laguna de Celestun, with discrete subgroups according to region. In contrast, fish from Laguna de Mecoacan were grouped together completely separate from the rest of the fish. Despite the low number of fish from Mecoacan (a high bootstrap support was observed in this group), the results indicated a significant genetic variability in comparison with the other ecosystems included. The differential level of expression of CYP1A and the EROD activity observed among the ecosystems analyzed could be due to the high range of genetic variation, with special emphasis on fish collected in Mecoacan where it is possible to find a subspecies of Ariopsis felis.


Assuntos
Peixes-Gato/genética , Citocromo P-450 CYP1A1/genética , Regulação Enzimológica da Expressão Gênica , Fígado/enzimologia , Polimorfismo de Fragmento de Restrição , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados/métodos , Animais , Peixes-Gato/metabolismo , Citocromo P-450 CYP1A1/biossíntese , Exposição Ambiental , Monitoramento Ambiental , Indução Enzimática/efeitos dos fármacos , Expressão Gênica , RNA Mensageiro/metabolismo , Especificidade da Espécie , Poluentes Químicos da Água/toxicidade
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