RESUMO

We report a 1-step assay to screen antiviral substances combining the simultaneous application of cells, virus sample, and drug to 96-well plates. The results were obtained within 26 h when vaccinia virus plaques were counted or virus-induced cytopathic effect was measured. This fast cost-effective procedure may be used for other cytopathic viruses and is suitable to 1st screening tests.

Assuntos

Antivirais/isolamento & purificação , Avaliação Pré-Clínica de Medicamentos/economia , Avaliação Pré-Clínica de Medicamentos/métodos , Vaccinia virus/efeitos dos fármacos , Animais , Linhagem Celular , Análise Custo-Benefício , Efeito Citopatogênico Viral , Haplorrinos , Humanos , Ensaio de Placa Viral