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The present study observed significant effects of whole-body vibration (WBV) on bone mineral density (BMD) in postmenopausal women, with high-quality evidence for high-frequency, low-magnitude, and high-cumulative-dose use. The aim was to update a previous systematic review with meta-analysis to observe the effects of WBV on BMD in postmenopausal women. For the meta-analysis, the weighted mean difference between WBV and control groups, or WBV and conventional exercise, was used for the area of bone mineral density (aBMD) of the lumbar spine, femoral neck, total hip, trochanter, intertrochanter, and Ward's area, or volumetric trabecular bone mineral density (vBMDt) of the radius and tibia. Methodological quality was assessed using the PEDro scale and the quality of evidence using the GRADE system. In total, 23 studies were included in the systematic review and 20 in the meta-analysis. Thirteen studies showed high methodological quality. WBV compared with control groups showed significant effects on aBMD in the primary analysis (lumbar spine and trochanter), sensitivity (lumbar spine), side-alternating vibration (lumbar spine and trochanter), synchronous vibration (lumbar spine), low frequency and high magnitude (lumbar spine and trochanter), high frequency and low magnitude (lumbar spine), high frequency and high magnitude (lumbar spine, trochanter, and Ward's area), high cumulative dose and low magnitude (lumbar spine), low cumulative dose and high magnitude (lumbar spine and trochanter), and positioning with semi-flexed knees (trochanter). Of these results, only high frequency associated with low magnitude and high cumulative dose with low magnitude showed high-quality evidence. At this time, considering the high quality of evidence, it is possible to recommend WBV using high frequency (≈ 30 Hz), low magnitude (≈ 0.3 g), and high cumulative dose (≈ 7000 min) to improve lumbar spine aBMD in postmenopausal women. Other parameters, although promising, need to be better investigated, considering, when applicable, the safety of the participants, especially in vibrations with higher magnitudes (≥ 1 g).

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Radiofrequency (RF) treatment appears to be involved in production of new collagen fibrils and the improvement of existing collagen structures; however, the molecular bases of the effect of non-invasive RF on the skin tissue have not been fully elucidated. This study reports the effects of RF associated or not with hydrolyzed collagen (HC) in the skin tissue. Wistar rats were randomly divided into four groups, according to the treatment received: control group (G1, n = 5), no treatment; subjects in group G2 (n = 5) were treated with HC; and capacitive RF was applied to the back of each subject in G3 (n = 5) and RF associated with HC in G4 (n = 5). Biopsies were taken 30 days after treatment and then were histologically processed and studied for inflammatory cell counting, collagen content, and morphometry. In addition, FGF2, CD105, and COX-2 expression was assessed by immunohistochemical staining. The most relevant changes were the increase in cellularity and accumulation of intercellular substance in RF-treated animals (G3 and G4). The greatest dermis thickness rate was observed in G4, followed by G3 and G2 (p < 0.05). RF-treated skins (G3 and G4) exhibited a significant overexpression of FGF2 (p < 0.0001) and increased microvessel density (p < 0.0001) in comparison with G1 and G2. Moreover, the amount of COX-2 was significantly higher (p < 0.0001) in dermis of RF-treated areas compared to G1 and G2, and demonstrated differences in G3 (RF) compared to G4 (RF + HC) (p < 0.0001). Our results suggests that RF treatment associated or not with HC induces FGF2 overexpression, promotes neoangiogenesis and modulates the COX-2 expression, subsequently promotes neocollagenesis, and increased thickness rate of dermis.

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There is a growing interest in the use of vibration generated by oscillating/vibratory platforms - also known as whole-body vibration (WBV) - for achieving therapeutic, preventative and/or physical performance goals. This study investigated the effects of vibration generated by an oscillating platform on the concentration of blood biomarkers in rats. Wistar rats (n = 8) were divided in 2 groups, sedated and individually positioned on an oscillating platform. The experimental group (EG) was subjected to vibrations of 20 Hz for one min per day for one week while the control group (CG) experienced no vibration. Samples of heparinized whole blood were drawn by cardiac puncture for biochemical analysis. Concentrations of total cholesterol, triglycerides, HDL, LDL, VLDL, glucose, CK, albumin, alkaline phosphates, TGP, TGO, γGT, lipase, amylase, urea and creatinine were determined. White blood cell count and a platelet hemogram were also performed. Following seven sessions of exposure to the vibration, a significant (P < 0.05) reduction in γGT, VLDL and leukocytes was found. A weekly 1-min/day exposure of 20 Hz vibration can was shown to alter the concentrations of selected blood biomarkers in rats. The action mechanism associated with these effects seems highly complex, but the findings might contribute to the understanding of these mechanisms related to the exposure to 20 Hz vibration.

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PURPOSE: The radionuclide bone scan is the basis of skeletal nuclear medicine imaging. Bone scintigraphy is a highly sensitive method for indicating disease in bone. Mechanical stimulation in the manner of whole-body vibration (WBV) appears beneficial to the maintenance and/or enhancement of skeletal mass in individuals. The aim of this work was to evaluate the effect of WBV on the biodistribution of the radiopharmaceutical [99mTc]methylene diphosphonate (99mTc-MDP) in Wistar rats. MATERIALS AND METHODS: In the biodistribution analysis, animals were anesthetized with sodium thiopental, the radiopharmaceutical (99m)Tc-MDP was administered via ocular plexus and after 10 min the animals were submitted to vibration of 20 Hz (1 min) in an oscillatory platform. Following, the animals were sacrificed, the organs were isolated, the radioactivity determined in a well counter, and the percentages of radioactivity per gram (%ATI/g) in the organs were calculated. An unpaired t-test following Welch test (p < 0.05) was done for statistical analysis of the results. RESULTS: The biodistribution was significantly (p < 0.05) decreased in kidney, bone, lung, stomach, prostate and bowel. CONCLUSION: The analysis of the results indicates that the vibration could produce metabolic alterations with influence in the uptake of the radiopharmaceutical 99mTc-MDP in bone, stomach, bowel, prostate, kidney and bladder.

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Radiopharmaceuticals are employed in patient diagnostics and disease treatments. Concerning the diagnosis aspect, technetium-99m (99mTc) is utilized to label radiopharmaceuticals for single photon computed emission tomography (SPECT) due to its physical and chemical characteristics. 99mTc fixation on pharmaceuticals depends on a reducing agent, stannous chloride (SnCl(2)) being the most widely-utilized. The genotoxic, clastogenic and anegenic properties of the 99mTc-MDP(methylene diphosphonate used for bone SPECT) and SnCl(2) were evaluated in Wistar rat blood cells using the Comet assay and micronucleus test. The experimental approach was to endovenously administer NaCl 0.9% (negative control), cyclophosphamide 50 mg/kg b.w. (positive control), SnCl(2) 500 μg/mL or 99mTc-MDP to animals and blood samples taken immediately before the injection, 3, and 24 h after (in the Comet assay) and 36 h after, for micronucleus test. The data showed that both SnCl(2) and 99mTc-MDP-induced deoxyribonucleic acid (DNA) strand breaks in rat total blood cells, suggesting genotoxic potential. The 99mTc-MDP was not able to induce a significant DNA strand breaks increase in in vivo assays. Taken together, the data presented here points to the formation of a complex between SnCl(2) in the radiopharmaceutical 99mTc-MDP, responsible for the decrease in cell damage, compared to both isolated chemical agents. These findings are important for the practice of nuclear medicine.

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The objective of this work was to investigate the effects of whole-body vibration on people with multiple sclerosis (MS). PubMed, CINAHL and Scopus databases were systematically searched for studies on the use of whole-body vibration (WBV) exercise in people with MS. These searches were supplemented with material identified in the references and in the authors' personal files. A qualitative analysis was performed to summarize the findings. Five studies with a total of seventy-one subjects were identified. All of these studies had small numbers of subjects (3-25), and two of the studies had no control groups. Some investigations have shown significant improvements of the muscle strength, of the functional mobility, and of the timed get up and go test in patients with MS. The number of publications found in the databanks searched is small, and in general, they have limitations in the design of protocols with a weakness to the interpretation of the findings. However, the analysis of the findings in these studies permits to conclude that some papers indicate that WBV exercises could benefit patients with MS. In addition, we suggest further larger scale investigations with controlled parameters and well-designed protocols into the effects of WBV exercises in people with MS.

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This study aimed at collecting data of masseter myofascial tension, related to the joint hyperactivity by electromyography activity of masseter muscle at rest, isotonic and isometric chewing. 08 patients living in the city of Buenos Aires, Argentina, volunteers, aged between 22 and 62 years, both sexes were evaluated. The electromyography data collection at rest had reported that 50% showed differences in masseter muscle tension increased in the left for the right masseter; in isometric contraction, 50% had a difference in masseter tension right; in isotonic contraction 62.5% there was a difference in increased tension in the left masseter. Anthropometry of the face was suggested, where 25% showed significant variance of 01 cm of discrepancy on the right, in relation to the left. It is possible in the future, an early diagnosis and treatment, thus avoiding a source of pain, speech disorders and problems in the mastication system. For this we need to establish a direct follow-up control of at least one year after the treatment strategy.

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Among nonpharmacological strategy to manage fibromyalgia, exercise (aerobic) has shown efficacy. Whole-body vibration (WBV) exercise has been proposed as a potential clinical intervention. WBV would induce increase in growth hormone (GH). An impairment of the hypothalamic-pituitary-GH-Insulin Growth Factor-1(IGF-1) axis has been implicated in the pathophysiology of fibromyalgia. This article aims to review the studies on exploring the relationship between WBV and fibromyalgia. Literature searches were performed in the PubMed database on 04/03/2010 using terms related to "pain", "whole body vibration" and "fibromyalgia". An important number of publications were identified with the term "pain" and in comparison, only a small number of articles were found related to "fibromyalgia". Three publications found with "whole body vibration" and fibromyalgia were analyzed.There are reports describing increase in serum IGF-1 following exposure to WBV in elderly patients. However, one randomized fibromyalgia trial revealed no changes in serum IGF-1 levels in women undergoing WBV. Due to the paucity of available, effective therapies for fibromyalgia, further studies that explore the relationship between the neuroendocrine system, fibromyalgia and WBV are merited.

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We evaluated the in vitro effect of an aqueous extract of Ginkgo biloba (EGb) on the distribution in blood cells (BC) and plasma (P) and on the binding of Na(123)I to the blood constituents using precipitation with trichloroacetic acid. The radioactivity percentages insoluble (SF) and insoluble fraction (IF) of blood constituents were determined. The EGb interfered (p<0.05) on the distribution of Na(123)I in the P (from 69.64 to 86.13) and BC (from 30.36 to 13.87) and altered the fixation of the Na(123)I in IF-P and in IF-BC.

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Atualmente, profissionais de várias áreas têm aumentado seu interesse no conhecimento da articulação temporomandibular, uma das mais complexas articulações do corpo humano por sua anatomia, sua capacidade de movimentação, assim como por serem duas articulações em um único osso. A principal característica da dor miofascial inclui fraqueza muscular e pontos de dor, chamados trigger points, dor local e referida. Objetivo: Esse estudo objetiva coletar dados de tensão miofascial do masseter, relacionada à hiperatividade da junção por atividade eletromiográfica do músculo masseter em repouso, e em contração isotônica e em contração isométrica. Métodos: Foram avaliados 8 pacientes que viviam na cidade de Buenos Aires, Argentina, voluntários, com idade entre 22 e 62 anos, ambos os sexos, por eletromiografia quando do músculo masseter em repouso ou em contração isotônica ou isométrica. Resultados: A coleção de resultados eletromiográficos ao repouso mostrou que 50% apresentaram diferenças na tensão muscular do masseter esquerdo que foi maior que a do masseter direito. Em contração isométrica, 50% tinham diferença na tensão do masseter direito. Em contração isotônica 62,5% tinham diferença no aumento de tensão no masseter esquerdo. Foi realizada a antropometria da face, onde 25% mostraram uma variação significante de Olcm de discrepância no lado direito, em relação ao esquerdo. Conclusão: No futuro através de um diagnóstico e tratamento tardios, que livrem o paciente da dor, das desordens de velocidade de mastigação, que para isso necessitarão estabelecer um controle direto de pelo menos 1 ano após a estratégia de tratamento.

Introduction: Currently, professionals from various areas of expertise had grown interest in knowing the temporomandibular joint, one of the most complex joint of the human body for its anatomy, its handling capacity, as well as being two joints in a single bone. The principal characteristic of myofascial pain includes muscle weakness and pain point, called trigger points, local and referred pain. Objective: This study aimed to collect data of masseter myofascial tension, related to the joint hyperactivity by electromyography activity of masseter muscle at rest, and at isotonic or isometric contraction. Methods: Were evaluated 08 patients living in the city of Buenos Aires, Argentina, volunteers, aged between 22 and 62 yeras, both sex, by electromyography when the masseter muscle was in rest, or in isotonic or isometric constraction Results: The electromyography data collection at rest had reported that 50% showed diferences in masseter muscle tension increased in the left for the right masseter; in isometric contraction, 50% had difference in masseter tension right; in isotonic contraction 62.5% had difference increased tension in the left masseter. It was done the anthropometry of the face, where 25% showed significant variance of 01 cm of discrepancy on the right, in relation to the left. Conclusion: In the future through an early diagnosis and treatment, that avoiding a source of pain and disorders of speech mastication, that needs to establish a direct control at least one year after the treatment strategy.

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