RESUMO

Organic fertilization and mycorrhization can increase the phytoremediation of copper-contaminated soils. The time of vermicomposting alters the properties of vermicompost, which can affect copper's availability and uptake. Therefore, this study sought to evaluate the effect of different organic fertilizers and mycorrhization on copper-contaminated soil phytoremediation. The soil was contaminated with 100 mg Cu kg-1 dry soil and received mineral fertilizer (MIN), bovine manure (CM), and vermicompost produced in 45 days (V45) or 120 days (V120), all in doses equivalent to 40 mg kg-1 dry soil of phosphorus. Half of the jack bean (Canavalia ensiformis) plants were inoculated with the arbuscular mycorrhizal fungus Rhizophagus clarus. At plant flowering, the dry mass and concentrations of Cu, Zn, Mn, Ca, Mg, P, and K in the soil, solution, and plant tissue were determined, in addition to mycorrhizal colonization, nodulation, photosynthetic pigments, and oxidative stress enzyme activity. Organic fertilization increased plant growth and copper accumulation in aerial tissues. These effects were more evident with the V120, making it suitable for use in copper phytoextraction. Mycorrhization increased root and nodule dry mass, making it recommended for phytostabilization. C. ensiformis nodulation in Cu-contaminated soils depends on vermicompost fertilization and mycorrhization. Hence, the copper phytoremediation by C. ensiformis is increased by using organic fertilization and mycorrhization.

Assuntos

RESUMO

Chemical and physical properties of soil can be altered with the successive application of liquid swine manure (LSM), also known as slurry. Therefore, monitoring the impact of LSM is essential to an assessment of the potential agronomic and environmental benefits and risks associated with management practices. The aim of this study was to evaluate the effects of LSM on the chemical and physical attributes of soil under pasture (Cynodon spp.), located in southern Brazil. Four areas were sampled: three areas cultivated with Cynodon spp. pasture with successive applications of 200 m3 ha1 y1 of LSM for three, eight and 15 years; and untreated native forest as a reference. The soil attributes evaluated were: organic carbon (OC), active (pH water) and potential acidity (H + Al+ 3), nutrient availability (i.e., P, K, Ca, Mg, Cu and Zn), soil bulk density, macro, micro and total porosity. Successive applications of LSM on pasture reduced soil active acidity, and increased the soil organic C and plant-available N, P, Ca, Mg, Cu and Zn contents. These effects were more intense with time and in the shallowest layers, i.e. 0-10 cm. Excessive increases in P, Cu and Zn in the soil should be carefully monitored to minimize the contamination risks to soil, ground and surface waters. Soil physical attribute changes were more associated with land use (pasture versus native forest) than LSM use, and LSM applications induced slight improvements in the soil structural quality over time.

Assuntos

RESUMO

Chemical and physical properties of soil can be altered with the successive application of liquid swine manure (LSM), also known as slurry. Therefore, monitoring the impact of LSM is essential to an assessment of the potential agronomic and environmental benefits and risks associated with management practices. The aim of this study was to evaluate the effects of LSM on the chemical and physical attributes of soil under pasture (Cynodon spp.), located in southern Brazil. Four areas were sampled: three areas cultivated with Cynodon spp. pasture with successive applications of 200 m3 ha1 y1 of LSM for three, eight and 15 years; and untreated native forest as a reference. The soil attributes evaluated were: organic carbon (OC), active (pH water) and potential acidity (H + Al+ 3), nutrient availability (i.e., P, K, Ca, Mg, Cu and Zn), soil bulk density, macro, micro and total porosity. Successive applications of LSM on pasture reduced soil active acidity, and increased the soil organic C and plant-available N, P, Ca, Mg, Cu and Zn contents. These effects were more intense with time and in the shallowest layers, i.e. 0-10 cm. Excessive increases in P, Cu and Zn in the soil should be carefully monitored to minimize the contamination risks to soil, ground and surface waters. Soil physical attribute changes were more associated with land use (pasture versus native forest) than LSM use, and LSM applications induced slight improvements in the soil structural quality over time.(AU)

Assuntos

RESUMO

The inclusion of winter cover crops and fertilization with nitrogen to the soil can have an effect on their biological and microbiological attributes. The aim of this study was to evaluate biological and microbiological attributes in soil under different winter cover crops and nitrogen doses. The experiment was conducted at the Frederico Westphalen-RS campus of the Federal University of Santa Maria (UFSM) in a Rhodic Hapludox soil. The experimental design was a randomized block in factorial arrangement (2 x 10): 10 winter cover crops systems (Fallow [control], black oats, white oats, ryegrass, forage turnip, vetch, white lupine; black oat + forage turnip; black oat + vetch and black oat + vetch + fodder turnip), and two nitrogen rates in the form of urea applied in successive crops of beans common and maize, with four replications. We assessed the biological attributes (Margalefs richness, Simpsons dominance, Shannons diversity and abundance of organisms) and microbiological (carbon and nitrogen microbial biomass, basal respiration, metabolic quotient and microbial quotient of the soil). The fallow with wild species and white lupine showed greater Simpsons dominance and abundance of organisms due to the increase in the number of individuals of the order Collembola. Vetch improved the biological attributes of the soil with increase in Collembola abundance and diversity of organisms of soil fauna. The application of nitrogen favored the microbial biomass carbon and reduced the metabolic quotient.(AU)

A inclusão de plantas de cobertura de inverno e adubação com nitrogênio (N) ao solo pode exercer efeito nos seus atributos biológicos e microbiológicos. O objetivo deste trabalho foi avaliar atributos biológicos e microbiológicos em solo submetido a diferentes plantas de cobertura de inverno e doses de nitrogênio. O trabalho foi conduzido no campus de Frederico Westphalen, RS da Universidade Federal de Santa Maria de abril de 2010 a outubro de 2013 em Latossolo Vermelho distrófico típico. O delineamento experimental foi em blocos ao acaso em arranjo fatorial (10 x 2) sendo 10 sistemas de plantas de cobertura de inverno: Pousio (testemunha com espécies espontâneas); Aveia preta; Aveia branca; Azevém; Nabo forrageiro; Ervilhaca; Tremoço branco; Aveia preta + Nabo forrageiro; Aveia preta + Ervilhaca e Aveia preta + Nabo forrageiro + Ervilhaca, e duas doses de nitrogênio na forma de ureia aplicados nos cultivos sucessivos de feijão e milho, com quatro repetições. Avaliaram-se os atributos biológicos da fauna edáfica (riqueza de Margalef, dominância de Simpson, diversidade de Shannon e densidade de organismos) e microbiológicos (carbono da biomassa microbiana, nitrogênio da biomassa microbiana, respiração basal, quociente metabólico e quociente microbiano do solo). O Pousio com espécies espontâneas e o tremoço resultaram em maior dominância de Simpson e densidade de organismos decorrente do aumento no número de indivíduos da ordem Collembola. A ervilhaca melhorou os atributos biológicos do solo com aumento no número de Collembola, abundância, riqueza e diversidade de organismos da fauna edáfica. A aplicação de nitrogênio favoreceu o carbono da biomassa microbiana e reduziu o quociente metabólico.(AU)

Assuntos

RESUMO

The inclusion of winter cover crops and fertilization with nitrogen to the soil can have an effect on their biological and microbiological attributes. The aim of this study was to evaluate biological and microbiological attributes in soil under different winter cover crops and nitrogen doses. The experiment was conducted at the Frederico Westphalen-RS campus of the Federal University of Santa Maria (UFSM) in a Rhodic Hapludox soil. The experimental design was a randomized block in factorial arrangement (2 x 10): 10 winter cover crops systems (Fallow [control], black oats, white oats, ryegrass, forage turnip, vetch, white lupine; black oat + forage turnip; black oat + vetch and black oat + vetch + fodder turnip), and two nitrogen rates in the form of urea applied in successive crops of beans common and maize, with four replications. We assessed the biological attributes (Margalefs richness, Simpsons dominance, Shannons diversity and abundance of organisms) and microbiological (carbon and nitrogen microbial biomass, basal respiration, metabolic quotient and microbial quotient of the soil). The fallow with wild species and white lupine showed greater Simpsons dominance and abundance of organisms due to the increase in the number of individuals of the order Collembola. Vetch improved the biological attributes of the soil with increase in Collembola abundance and diversity of organisms of soil fauna. The application of nitrogen favored the microbial biomass carbon and reduced the metabolic quotient.

A inclusão de plantas de cobertura de inverno e adubação com nitrogênio (N) ao solo pode exercer efeito nos seus atributos biológicos e microbiológicos. O objetivo deste trabalho foi avaliar atributos biológicos e microbiológicos em solo submetido a diferentes plantas de cobertura de inverno e doses de nitrogênio. O trabalho foi conduzido no campus de Frederico Westphalen, RS da Universidade Federal de Santa Maria de abril de 2010 a outubro de 2013 em Latossolo Vermelho distrófico típico. O delineamento experimental foi em blocos ao acaso em arranjo fatorial (10 x 2) sendo 10 sistemas de plantas de cobertura de inverno: Pousio (testemunha com espécies espontâneas); Aveia preta; Aveia branca; Azevém; Nabo forrageiro; Ervilhaca; Tremoço branco; Aveia preta + Nabo forrageiro; Aveia preta + Ervilhaca e Aveia preta + Nabo forrageiro + Ervilhaca, e duas doses de nitrogênio na forma de ureia aplicados nos cultivos sucessivos de feijão e milho, com quatro repetições. Avaliaram-se os atributos biológicos da fauna edáfica (riqueza de Margalef, dominância de Simpson, diversidade de Shannon e densidade de organismos) e microbiológicos (carbono da biomassa microbiana, nitrogênio da biomassa microbiana, respiração basal, quociente metabólico e quociente microbiano do solo). O Pousio com espécies espontâneas e o tremoço resultaram em maior dominância de Simpson e densidade de organismos decorrente do aumento no número de indivíduos da ordem Collembola. A ervilhaca melhorou os atributos biológicos do solo com aumento no número de Collembola, abundância, riqueza e diversidade de organismos da fauna edáfica. A aplicação de nitrogênio favoreceu o carbono da biomassa microbiana e reduziu o quociente metabólico.

Assuntos

RESUMO

O uso de espécies arbóreas nativas inoculadas com fungo ectomicorrízico pode ser uma alternativa para revegetação de solo contaminado com cobre. O objetivo do trabalho foi avaliar a interferência do óleo essencial de eucalipto, cobre e ectomicorriza na qualidade de mudas de timbaúva-preta (Enterolobium contorsiliquum) e pata-de-vaca (Bauhinia forficata). O trabalho foi conduzido em casa de vegetação por 120 dias, usando como substrato a camada de 0 20 cm de um Latossolo Vermelho com textura argilosa. O delineamento experimental foi inteiramente casualizado em arranjo fatorial (2 x 2 x 2), sendo com e sem inoculante ectomicorrízico, óleo essencial e adição de 150 mg kg-1 de cobre no solo. Avaliou-se a altura da planta, diâmetro de colo, massa seca da parte aérea, das raízes e total, comprimento e área superficial específica radicular e a qualidade das mudas pelas relações entre: altura da parte aérea e diâmetro do colo, altura da parte aérea e massa seca da parte aérea e pelo índice de qualidade de Dickson. A formação da ectomicorriza foi identificada pela visualização de estruturas fúngicas na morfologia externa e interna do sistema radicular. A adição de cobre reduziu o diâmetro do colo e massa seca da parte aérea das mudas de pata-de-vaca independente da adição de inoculante e óleo. Não houve formação de ectomicorriza, mesmo quando aplicado óleo essencial nas mudas da timbaúva-preta e pata-de-vaca. O crescimento das mudas da timbaúva-preta é estimulado pela adição de 150 mg kg-1 de cobre e apresenta maior índice de qualidade de mudas em relação a pata-de-vaca.(AU)

The use of native tree species inoculated with ectomycorrhizal fungus can be an alternative for revegetation of soil contaminated with copper. The objective was to evaluate the interference of eucalyptus essential oil copper and ectomycorrhiza on growth and quality of seedlings timbaúva-preta (Enterolobium contorsiliquum) and pata-de-vaca (Bauhinia forficata). The study was conducted in greenhouse for 120 days using as substrate the layer of 0-20 cm of a Red Oxisol with very clayey texture. The experimental design was completely randomized factorial (2 x 2 x 2), and with and without inoculant ectomycorrhizal essential oil and addition of 150 mg kg-1 of copper in the soil . Was evaluated the plant height, stem diameter, dry mass of shoots, roots and total length and specific surface area and root seedling quality of relations between: shoot height and stem diameter, shoot height and dry mass of shoots and the quality index of Dickson. The formation of ectomycorrhiza was identified by visualization of fungal structures and surface morphology internal and external morphology of the root system. Addition of copper reduced de stems diameter and dry mass of de aerial part of the pata-de-vaca seedlings regardless of the addition of inoculant and oil. There was no formation of ectomycorrhiza, even when applied in the essential oil of seedlings timbaúva-preta and pata-de-vaca. Growth of timbaúva-preta seedlings is stimulated by the addition of 150 mg kg 1 of copper and has a higher rate of seedling quality in relation to pata-de-vaca.(AU)

Assuntos

RESUMO

O uso de espécies arbóreas nativas inoculadas com fungo ectomicorrízico pode ser uma alternativa para revegetação de solo contaminado com cobre. O objetivo do trabalho foi avaliar a interferência do óleo essencial de eucalipto, cobre e ectomicorriza na qualidade de mudas de timbaúva-preta (Enterolobium contorsiliquum) e pata-de-vaca (Bauhinia forficata). O trabalho foi conduzido em casa de vegetação por 120 dias, usando como substrato a camada de 0 20 cm de um Latossolo Vermelho com textura argilosa. O delineamento experimental foi inteiramente casualizado em arranjo fatorial (2 x 2 x 2), sendo com e sem inoculante ectomicorrízico, óleo essencial e adição de 150 mg kg-1 de cobre no solo. Avaliou-se a altura da planta, diâmetro de colo, massa seca da parte aérea, das raízes e total, comprimento e área superficial específica radicular e a qualidade das mudas pelas relações entre: altura da parte aérea e diâmetro do colo, altura da parte aérea e massa seca da parte aérea e pelo índice de qualidade de Dickson. A formação da ectomicorriza foi identificada pela visualização de estruturas fúngicas na morfologia externa e interna do sistema radicular. A adição de cobre reduziu o diâmetro do colo e massa seca da parte aérea das mudas de pata-de-vaca independente da adição de inoculante e óleo. Não houve formação de ectomicorriza, mesmo quando aplicado óleo essencial nas mudas da timbaúva-preta e pata-de-vaca. O crescimento das mudas da timbaúva-preta é estimulado pela adição de 150 mg kg-1 de cobre e apresenta maior índice de qualidade de mudas em relação a pata-de-vaca.

The use of native tree species inoculated with ectomycorrhizal fungus can be an alternative for revegetation of soil contaminated with copper. The objective was to evaluate the interference of eucalyptus essential oil copper and ectomycorrhiza on growth and quality of seedlings timbaúva-preta (Enterolobium contorsiliquum) and pata-de-vaca (Bauhinia forficata). The study was conducted in greenhouse for 120 days using as substrate the layer of 0-20 cm of a Red Oxisol with very clayey texture. The experimental design was completely randomized factorial (2 x 2 x 2), and with and without inoculant ectomycorrhizal essential oil and addition of 150 mg kg-1 of copper in the soil . Was evaluated the plant height, stem diameter, dry mass of shoots, roots and total length and specific surface area and root seedling quality of relations between: shoot height and stem diameter, shoot height and dry mass of shoots and the quality index of Dickson. The formation of ectomycorrhiza was identified by visualization of fungal structures and surface morphology internal and external morphology of the root system. Addition of copper reduced de stems diameter and dry mass of de aerial part of the pata-de-vaca seedlings regardless of the addition of inoculant and oil. There was no formation of ectomycorrhiza, even when applied in the essential oil of seedlings timbaúva-preta and pata-de-vaca. Growth of timbaúva-preta seedlings is stimulated by the addition of 150 mg kg – 1 of copper and has a higher rate of seedling quality in relation to pata-de-vaca.

Assuntos