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The aim of this study was to test the reliability of a method to measure the masticatory performance of complete denture wearers employing a colour-mixing ability test and assessment by visual and electronic colourimetric analysis. A sample of 75 subjects was selected from patients who received new conventional complete dentures. Masticatory tests were performed using a two-colour chewing gum that was masticated for 5, 10, 20, 30 and 50 chewing cycles, performed in a random order. The mixing level of the two colours of the chewed gum was assessed visually by two independent raters based on a 5-point ordinal scale. The specimens were flattened into a 1-mm-width wafer, scanned and saved as a two-sided digital image. Each pair of images was submitted to an electronic colourimetric analysis to assess the level of colour mixture, measured by the circular variance of hue (VOH). Overall inter- and intra-rater agreements in visual analysis were 64% and 68%, respectively (almost 99% of scores ranged within ±1 point), whilst overall weighted kappa was >0.80. A proportional increase in the level of mixture occurred with increased number of chewing cycles (P < .001). Similarly, VOH and the visual analysis were highly correlated (r = -.89; P < .001). Bland-Altman plots revealed excellent agreement and extremely low systematic error between duplicated VOH measures. It was concluded that the two-colour chewing gum test is a reliable method to assess the masticatory performance in complete denture wearers using both visual and electronic colourimetric analyses.

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The Lomb-Scargle periodogram was introduced in astrophysics to detect sinusoidal signals in noisy unevenly sampled time series. It proved to be a powerful tool in time series analysis and has recently been adapted in biomedical sciences. Its use is motivated by handling non-uniform data which is a common characteristic due to the restricted and irregular observations of, for instance, free-living animals. However, the observational data often contain fractions of non-Gaussian noise or may consist of periodic signals with non-sinusoidal shapes. These properties can make more difficult the interpretation of Lomb-Scargle periodograms and can lead to misleading estimates. In this letter we illustrate these difficulties for noise-free bimodal rhythms and sinusoidal signals with outliers. The examples are aimed to emphasize limitations and to complement the recent discussion on Lomb-Scargle periodograms.

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With respect to the first example in Schimmel (2001), Van Dongen et al. (2001) conclude from their Lomb-Scargle analysis that the noise I used 'contains new periodicities that are added to the signal (these periodicities by themselves resemble a harmonic series of a 38-hour rhythm).' They infer that 'the variance of the added noise is about five times as large as the variance of the signal' causing the detection of the new significant periodicities in the noise prior to the 24-h bimodal rhythm. Moreover the 'example reflects a combination of an extremely non-sinusoidal signal with noise that is not independent, which results in a time series that is difficult to analyze with virtually any know method.' In the following, I briefly examine these concerns to avoid misunderstandings and to alert that with an adequate use of the statistical significance test, misleading conclusions can be obtained. Although this paper further emphasizes difficulties in the detection with Lomb-Scargle periodograms, this should not be used as de-motivation. As stated in Schimmel (2001) Lomb-Scargle is a powerful technique but such as any other method one should be aware about its limitations, and use additional tools to constrain the true data characteristics.

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This study investigated the self-image of individuals who donate bone marrow to strangers as part of the National Marrow Donor Program. Quantitative surveys were administered to donors before donating (N = 849), shortly after donating (N = 754), and a year after donating (N = 370). In addition, 52 donors were interviewed in-depth by telephone at the same three points in time. Many of these donors felt that by donating bone marrow they were actualizing a central trait in their identity. Thus, many donors believed that they were distinct from others in the centrality of the traits of helpfulness and generosity to their identities. This belief often stemmed from a strong emphasis on helping in their families of origin. Other qualities activated by donation included religious identity, being a helping professional, and desire to be a role model. Self-evaluation appeared to have been enhanced for some donors due to the donation.

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In this study, we investigated the psychosocial effects of unrelated marrow donation. Survey questionnaires were administered pre-donation, shortly post-donation, and 1 year post-donation to all donors through the National Marrow Donor Program over a 3-year period. Univariate, bivariate, and multivariate analyses were then performed. Donors were generally quite positive about the donation 1 year post-donation: 87% felt it was "very worthwhile" and 91% would be willing to donate again in the future. Marrow donors were more likely than kidney donors to feel better about themselves as a result of the donation (P < .001). Donors with longer collection times, in general, had less positive psychosocial outcomes from the donation. Donors who experienced lower back pain or difficulty walking as a result of the donation were more likely to experience the donation as more stressful and painful than expected, but no more likely to experience it as less positive emotionally than donors who did not experience these side effects.

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