RESUMO
A rod-shaped and Gram-stain-negative bacterial strain 9AT, was isolated from an air sample collected at King George Island, maritime Antarctica. Phylogenetic analysis based on 16S rRNA gene sequence reveals that strain 9AT belongs to the genus Hymenobacter and shows the highest similarity to Hymenobacter coccineus CCM 8649T (96.8â%). The DNA G+C content based on the draft genome sequence is 64.9 mol%. Strain 9AT is strictly aerobic, psychrophilic, catalase-positive, oxidase-positive and non-motile. Growth is observed at 0-20 °C (optimum 10 °C), pH 6.0-8.0 (optimum pH 7.0), and in the absence of NaCl. The predominant menaquinone of strain 9AT is MK-7 and the major fatty acids comprise Summed Feature 3 (C16â:â1 ω7c and/or C16â:â1 ω6c; 25.2â%), iso-C15â:â0 (23.2â%), C16â:â1 ω5c (11.6â%), Summed Feature 4 (anteiso-C17â:â1 B/iso-C17â:â1 I) (9.6â%) and anteiso-C15â:â0 (9.6â%). The polar lipid profile consists of the major lipid phosphatidylethanolamine and moderate to minor amounts of phosphatidylserine, unidentified aminolipids, aminophospholipids, aminophosphoglycolipids, polar lipids lacking a functional group and an unidentified phospholipid and a glycolipid. In the polyamine pattern sym-homospermidine is predominant. On the basis of the results obtained, strain 9AT is proposed as a novel species of the genus Hymenobacter, for which the name Hymenobacter caeli sp. nov. is suggested. The type strain is 9AT (=CCM 8971T=LMG 32109T=DSM 111653T).
Assuntos
Microbiologia do Ar , Bacteroidetes/isolamento & purificação , Ilhas , Regiões Antárticas , Bacteroidetes/classificação , Bacteroidetes/genética , Composição de Bases , DNA Bacteriano/genética , Genoma Bacteriano , Funções Verossimilhança , Filogenia , RNA Ribossômico 16S/genéticaRESUMO
A rod-shaped and Gram-stain-negative bacterial strain, 1BT, was isolated from an air sample collected at King George Island, maritime Antarctica. Strain 1BT is strictly aerobic, psychrophilic, catalase-positive, oxidase-positive and non-motile. Growth of strain 1BT is observed at 0-20 °C (optimum, 10 °C), pH 6.0-8.0 (optimum, pH 8.0) and in the presence of 0-1.0% NaCl (optimum, 0.5â% NaCl). Phylogenetic analysis based on 16S rRNA gene sequences places strain 1BT within the genus Hymenobacter and shows the highest similarity to Hymenobacter antarcticus VUG-A42aaT (97.5â%). The predominant menaquinone of strain 1BT is MK-7 and the major fatty acids (>10â%) comprise summed feature 3 (C16â:â1 ω7c and/or C16â:â1 ω6c; 32.5â%), iso-C15â:â0 (17.6â%) and anteiso C15â:â0 (12.3â%). The polar lipid profile consists of the major compounds phosphatidylethanolamine, phosphatidylserine, two unidentified aminolipids and one unidentified phospholipid. The DNA G+C content based on the draft genome sequence is 61.2 mol%. Based on the data from the current polyphasic study, 1BT represents a novel species of the genus Hymenobacter, for which the name Hymenobacter artigasi sp. nov. is suggested. The type strain is 1BT (=CCM 8970T=CGMCC 1.16843T).
Assuntos
Microbiologia do Ar , Cytophagaceae/classificação , Filogenia , Regiões Antárticas , Técnicas de Tipagem Bacteriana , Composição de Bases , Cytophagaceae/isolamento & purificação , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
Escherichia albertii is a recently discovered species with a limited number of well characterized strains. The aim of this study was to characterize four of the E. albertii strains, which were among 41 identified Escherichia strains isolated from the feces of living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia. Sequencing of 16S rDNA, automated ribotyping, and rep-PCR were used to identify the four E. albertii isolates. Phylogenetic analyses based on multi-locus sequence typing showed these isolates to be genetically most similar to the members of E. albertii phylogroup G3. These isolates encoded several virulence factors including those, which are characteristic of E. albertii (cytolethal distending toxin and intimin) as well as bacteriocin determinants that typically have a very low prevalence in E. coli strains (D, E7). Moreover, E. albertii protein extracts caused cell cycle arrest in human cell line A375, probably because of cytolethal distending toxin activity.
Assuntos
Escherichia/metabolismo , Animais , Regiões Antárticas , Charadriiformes/microbiologia , Chile , Eletroforese em Gel de Campo Pulsado/veterinária , Escherichia/genética , Escherichia/isolamento & purificação , Fezes/microbiologia , Tipagem de Sequências Multilocus/veterinária , Reação em Cadeia da Polimerase/veterinária , RNA Ribossômico 16S/genética , Ribotipagem/veterinária , Focas Verdadeiras/microbiologia , Spheniscidae/microbiologiaRESUMO
Three yeast strains, which are phenotypically indistinguishable from Debaryomyces hansenii, were recovered from secondary mineral deposits (stalactites and stromatolites) obtained in the Crystal Eyes Cave, Roraima Tepui Mountain, Venezuela. Analyses of the D1/D2 domains of the LSU rRNA gene as well as the concatenated sequences of the nearly entire SSU rRNA gene, the ITS regions and the D1/D2 domains of the LSU rRNA gene confirmed the placement of these strains in the genus Debaryomyces, but relationship with all valid species of D. hansenii complex was distant. Based on the observed considerable sequence divergence the three strains are proposed as a new species, D. psychrosporus sp. nov., with the type strain NCAIM Y.01972(T) (CBS 11845(T), NRRL Y-48723(T)).
Assuntos
Debaryomyces/classificação , Debaryomyces/genética , Dados de Sequência Molecular , Filogenia , RNA RibossômicoRESUMO
Eight coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococcal strains were isolated from the gastrointestinal tracts of South American squirrel monkeys (Saimiri sciureus L.). These strains were differentiated from known staphylococcal species on the basis of 16S rRNA gene and hsp60 gene sequencing, and from the most closely related species by using DNA-DNA hybridization, ribotyping, whole-cell protein profiles and biotyping. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that these strains are members of the Staphylococcus aureus species group (99% similarity) but are biochemically similar to Staphylococcus piscifermentans, from which they can be phenotypically distinguished by resistance to polymyxin B, acid production from D-mannitol, the inability to hydrolyse aesculin and DNA and the absence of alpha-glucosidase. On the basis of these analyses, a novel species of the genus Staphylococcus is described, for which the name Staphylococcus simiae sp. nov. is proposed, with CCM 7213(T) (=LMG 22723(T)) as the type strain.