RESUMO

Pseudomonas aeruginosa belong to the special pathogen group capable of causing serious infections, with high mortality rates. The aim of this study was to describe the antibiotic resistance and genomic characteristics of Pseudomonas aeruginosa belonging to international high-risk clone ST235 (GPAE0131 isolate), obtained from hospital wastewater. P. aeruginosa GPAE0131 was isolated from ward tertiary hospital in Brazil and the antibiotic resistance profile was determined by the disc-diffusion method. Genomic characteristics related to antibiotic resistance and virulence factors were evaluated by genomic DNA sequencing on the Illumina MiSeq platform and bioinformatic analysis. GPAE0131 isolate showed resistance to piperacillin-tazobactam, cefepime, ceftazidime, imipenem, meropenem, ciprofloxacin, levofloxacin and tobramycin. Resistome comprehend of resistance genes to ß-lactams (blaVIM-2, blaOXA-4, blaOXA-488, blaPDC-35), aminoglycosides (aph(3')-IIb, aac(6')-IIc, aac(6')-Ib9, aadA1), fosfomycin (fosA), chloramphenicol (catB7) and sulfonamides (sul1). Genome comparisons evidence insertion of blaVIM-2 and blaOXA-4 genes. GPAE0131 isolate was predicted to be pathogenic to humans and several virulence factors were found, including encoding gene for ExoU and exotoxin A. All of these features into a pathogenic international high-risk clone (ST235), classified as critical priority, stands out as public health concern due to the widespread dispersal of human pathogens through wastewater. It is suggested that mitigating measures be implemented, such as the treatment of hospital sewage and the addition of tertiary treatment, to prevent the escape of pathogens at this level into the environment.

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RESUMO

Fish is an important source of animal protein for local communities in the Amazon basin, whose food safety must be assured. However, certain potential toxicants elements, can bioaccumulate in fish species, which inhabit anthropogenically polluted waters, ultimately posing a risk to human health. In the present study, the concentrations of nine elements (Al, As, Cd, Cr, Cu, Hg, Ni, Pb and Zn) were determined in raw and cooked samples of eight fish species consumed in Santarém (northern Brazil, Amazon biome). The potential for non- carcinogenic human health risks linked to the consumption of cooked fish were evaluated for adults and children in two different scenarios. Four carnivores, three omnivores and one detritivore, all of them regularly marketed and consumed by the Santarém population, were the target species. The safety reference values set by national and international guidelines for humans, in both raw and cooked preparations, were used. In most cases, the cooking process showed a trend to increase elements concentrations compared to raw samples, however the differences were not significant. Moreover, the risk assessment showed danger for children in relation to Hg from the consumption of fish, in both scenarios evaluated. For adults, in one of the scenarios, there was a health risk associated to Hg as a result of carnivorous fish consumption. In a context of combined exposure to all elements, children were at risk when consuming fish, especially carnivorous and omnivorous species. For adults, the mixture of elements posed a risk to health human only for carnivorous fish consumption. The results reveal an environmental scenario of Hg contamination, which requires monitoring actions to preserve the aquatic biodiversity and human health in the Brazilian Amazon biome.

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RESUMO

In the Amazon region, cities such as Santarém, in the state of Pará, Brazil, still lack Water Treatment Stations to serve the entire population. In these places, household methods of water disinfection are important to preserve potability and avoid undesirable health effects. Our study experimentally evaluated the effect of household methods for eliminating Escherichia coli in water samples. The techniques evaluated for this study were: (i) sodium hypochlorite 2.5%; (ii) boiling; (iii) ceramic filter, and (iv) sun exposure. Samples were tested, combining different concentrations of E. coli (from 3 to 100 colony forming units/100mL). The results showed that household disinfection methods were effective in eliminating E. coli; except for the ceramic filter, the water of which was still positive for their growth, even after filtration. Considering that the distribution of treated water does not reach most of the population living in Santarém and in peri-urban areas, such as the quilombola and riverside communities, the use of such methods as sodium hypochlorite 2.5%, boiling, and sun exposure may favor health promotion and reduce the occurrence of outbreaks of dysentery transmitted by water.

Na região Amazônica, cidades como Santarém, no Estado do Pará, Brasil, ainda carecem de Estações de Tratamento de Água para atender toda a população. Nesses locais, medidas domiciliares de desinfecção da água são importantes para preservar a potabilidade e evitar efeitos indesejáveis na saúde. Este estudo avaliou experimentalmente o efeito das medidas domiciliares na eliminação de Escherichia coli em amostras de água. As técnicas avaliadas para esse trabalho foram: (i) hipoclorito de sódio 2,5%; (ii) fervura; (iii) filtro de cerâmica; e (iv) exposição solar. Foram testadas amostras, combinando-se diferentes concentrações de E. coli (entre 3 e 100 unidades formadoras de colônias/100mL). Os resultados mostraram que as medidas domiciliares de desinfecção foram eficazes na eliminação da E. coli, com exceção do filtro de cerâmica, cujas amostras de água, mesmo após a filtragem, apresentaram-se positivas para o crescimento da bactéria. Considerando que a distribuição da água tratada não chega à maior parte da população que reside em Santarém e em áreas periurbanas, como em comunidades quilombolas e ribeirinhas, o uso das medidas como hipoclorito de sódio 2,5%, fervura e exposição solar poderão favorecer a promoção da saúde e diminuir a ocorrência de surtos de doenças diarreicas veiculadas pela água.

En la región Amazónica, las ciudades como Santarém, en el estado de Pará, Brasil, todavía carecen de Estaciones de Tratamiento de Agua para atender a toda la población. En estos lugares, las medidas de desinfección domiciliaria del agua son importantes para preservar la potabilidad y evitar efectos indeseables en la salud. Este estudio evaluó el efecto de medidas domiciliarias en la eliminación de Escherichia coli en muestras de agua. Las técnicas evaluadas en este trabajo fueron: (i) hipoclorito de sodio al 2,5%; (ii) hervir el agua; (iii) filtro cerámico y (iv) exposición al sol. Se probaron muestras combinando diferentes concentraciones de E. coli (entre 3 y 100 unidades formadoras de colonias/100mL). Los resultados mostraron que las medidas de desinfección domiciliaria fueron efectivas en la eliminación de E. coli, con excepción del filtro cerámico que presentó un resultado positivo para su crecimiento en las muestras de agua, aún después de filtrada. Considerando que la distribución de agua tratada no llega a la mayoría de la población residente en Santarém y en áreas periurbanas como comunidades quilombolas y ribereñas, el uso de técnicas como el hipoclorito de sodio al 2,5%; hervir el agua o exponerla al sol pueden favorecer una promoción de la salud y reducir el surgimiento de brotes de enfermedades diarreicas transmitidas por el agua.

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RESUMO

Klebsiella quasipneumoniae subsp. similipneumoniae has emerged as a human pathogen and sporadic isolates from non-clinical sources were reported. Here, we described the phenotypic- and genomic-characteristics of a multidrug-resistant (MDR) and potentially hypervirulent (MDR-hv) Klebsiella quasipneumoniae subsp. similipneumoniae (KqA1) isolated from hospital wastewater. The antibiotic susceptibility profile of KqA1 was investigated using disk-diffusion method, broth microdilution method, and agar dilution method, and the genetic characteristics of antimicrobial resistance, mobile genetics elements, and virulence were evaluated by genomic DNA sequencing on the Illumina® NovaSeq6000 platform as well as by bioinformatic analysis. Resistome analyses revealed the presence of genes related to resistance to ß-lactams, aminoglycosides, quinolones, tetracyclines, sulfonamides, trimethoprim, chloramphenicol, macrolides, and fosfomycin. New genetic contexts to blaGES-16 (carbapenemase gene) and to fosA (fosfomycin resistance gene) were described. A set of mechanisms that can contribute to antibiotic resistance, commonly detected in Klebsiella spp., was also found including chromosomal mutations, efflux systems, proteins, and regulators. Moreover, KqA1 presented genes related to tolerance to metals (arsenic, copper, nickel, cobalt, magnesium, cadmium, zinc, tellurium, selenium) and to biocides (quaternary-ammonium compounds). The isolate was classified as potentially hypervirulent due to a wide range of virulence factors found associated to regulation, motility, biofilm, effector delivery systems, immune modulation, nutritional/metabolic factors, adherence, invasion, and competitive advantage. The occurrence of MDR-hv KqA1 in hospital wastewater points out how this environment matrix plays a crucial role in the maintenance and selection of critical bacterial pathogens. Regarding One Health perspective, it is evident the need for multidisciplinary implementation of control measures for antibiotic-resistant bacteria, not only in hospital settings but also in a general environmental context to mitigate the dissemination of MDR and hv bacteria.

Assuntos

RESUMO

Na região Amazônica, cidades como Santarém, no Estado do Pará, Brasil, ainda carecem de Estações de Tratamento de Água para atender toda a população. Nesses locais, medidas domiciliares de desinfecção da água são importantes para preservar a potabilidade e evitar efeitos indesejáveis na saúde. Este estudo avaliou experimentalmente o efeito das medidas domiciliares na eliminação de Escherichia coli em amostras de água. As técnicas avaliadas para esse trabalho foram: (i) hipoclorito de sódio 2,5%; (ii) fervura; (iii) filtro de cerâmica; e (iv) exposição solar. Foram testadas amostras, combinando-se diferentes concentrações de E. coli (entre 3 e 100 unidades formadoras de colônias/100mL). Os resultados mostraram que as medidas domiciliares de desinfecção foram eficazes na eliminação da E. coli, com exceção do filtro de cerâmica, cujas amostras de água, mesmo após a filtragem, apresentaram-se positivas para o crescimento da bactéria. Considerando que a distribuição da água tratada não chega à maior parte da população que reside em Santarém e em áreas periurbanas, como em comunidades quilombolas e ribeirinhas, o uso das medidas como hipoclorito de sódio 2,5%, fervura e exposição solar poderão favorecer a promoção da saúde e diminuir a ocorrência de surtos de doenças diarreicas veiculadas pela água.

In the Amazon region, cities such as Santarém, in the state of Pará, Brazil, still lack Water Treatment Stations to serve the entire population. In these places, household methods of water disinfection are important to preserve potability and avoid undesirable health effects. Our study experimentally evaluated the effect of household methods for eliminating Escherichia coli in water samples. The techniques evaluated for this study were: (i) sodium hypochlorite 2.5%; (ii) boiling; (iii) ceramic filter, and (iv) sun exposure. Samples were tested, combining different concentrations of E. coli (from 3 to 100 colony forming units/100mL). The results showed that household disinfection methods were effective in eliminating E. coli; except for the ceramic filter, the water of which was still positive for their growth, even after filtration. Considering that the distribution of treated water does not reach most of the population living in Santarém and in peri-urban areas, such as the quilombola and riverside communities, the use of such methods as sodium hypochlorite 2.5%, boiling, and sun exposure may favor health promotion and reduce the occurrence of outbreaks of dysentery transmitted by water.

En la región Amazónica, las ciudades como Santarém, en el estado de Pará, Brasil, todavía carecen de Estaciones de Tratamiento de Agua para atender a toda la población. En estos lugares, las medidas de desinfección domiciliaria del agua son importantes para preservar la potabilidad y evitar efectos indeseables en la salud. Este estudio evaluó el efecto de medidas domiciliarias en la eliminación de Escherichia coli en muestras de agua. Las técnicas evaluadas en este trabajo fueron: (i) hipoclorito de sodio al 2,5%; (ii) hervir el agua; (iii) filtro cerámico y (iv) exposición al sol. Se probaron muestras combinando diferentes concentraciones de E. coli (entre 3 y 100 unidades formadoras de colonias/100mL). Los resultados mostraron que las medidas de desinfección domiciliaria fueron efectivas en la eliminación de E. coli, con excepción del filtro cerámico que presentó un resultado positivo para su crecimiento en las muestras de agua, aún después de filtrada. Considerando que la distribución de agua tratada no llega a la mayoría de la población residente en Santarém y en áreas periurbanas como comunidades quilombolas y ribereñas, el uso de técnicas como el hipoclorito de sodio al 2,5%; hervir el agua o exponerla al sol pueden favorecer una promoción de la salud y reducir el surgimiento de brotes de enfermedades diarreicas transmitidas por el agua.

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RESUMO

Gastrointestinal diseases caused by protozoan parasites remain a major challenge in developing countries and ingestion of contaminated surface water represents one of the main sources by which these diseases are contracted. This study assessed the risk of infection and diseases caused by Cryptosporidium spp. and Giardia sp. due to ingestion of surface water used for public supply and recreational activities, focusing on the southeastern Brazilian Pardo River and applying the USEPA 1623 method to quantify (oo)cyst concentrations. Infection and disease probabilities due to ingestion of drinking water or during recreational activities were estimated using the Quantitative Microbial Risk Assessment (QMRA) approach. Mean concentrations of Cryptosporidium spp. and Giardia sp. in surface water ranged from 0.2 to 0.4 oocysts L-1 and 0.2 to 4.4 cysts L-1, respectively. Considering public water supply, annual infection probabilities were higher for adults than children and exceeded the USEPA limit; also, disease probabilities were higher for adults than children. For recreational activities, annual infection and disease probabilities were higher for children, followed by men and women. The occurrence of both parasites likely reflects raw sewage discharge, effluent from sewage treatment plants, and diffuse sources of pollution, such as runoff from pasture lands and deforested riparian forest corridors. Our results highlight substantial infection risks by both parasite types after conventional treatment of water used for public supply and also call for careful monitoring of water bodies used for recreational purposes.

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