RESUMO
The effect of lead exposure in nitric oxide synthase containing neurons (nNOS) within rat cortex and hippocampus was studied. Lead administration (1 g% lead acetate in drinking water) was commenced prior to mating and continued until 30 postnatal (PN) days. Immunohistochemical studies using antibody to nNOS showed, after lead treatment at PN21-PN30, a reduction in neuronal size and optical density (OD) of nNOS+ cells. In both regions, non-pyramidal immunoreactive neurons exhibited smaller soma size and less developed dendrites. A significant difference in cell areas and OD of lead exposed versus control rats and no variation in the number of nNOS+ neurons was seen. Morphological modifications after early lead exposure, induced nNOS reduction in NOS expressing neurons thereby interfering in NO synthesis.
Assuntos
Córtex Cerebral/metabolismo , Hipocampo/metabolismo , Intoxicação por Chumbo/metabolismo , Óxido Nítrico Sintase/metabolismo , Animais , Animais Recém-Nascidos , Córtex Cerebral/efeitos dos fármacos , Dendritos/efeitos dos fármacos , Hipocampo/efeitos dos fármacos , Imuno-Histoquímica , Masculino , Ratos , Ratos Sprague-Dawley , Fatores de TempoRESUMO
Perinatal asphyxia (PA) produces changes in nitric oxide synthase (NOS) activity in neuronal and endothelial cells of the striatum and neocortex. The changes were examined using a histochemical NADPH-diaphorase (NADPH-d) staining method. Newborn rats were exposed to severe PA at 37 degrees C and other groups were subjected to severe PA under hypothermic condition (15 degrees C) for 20 or 100 min, respectively. Quantitative image analysis was performed on the striatum and neocortex in order to count cell number of reactive neurons and to compare the pattern of staining between the different groups of animals. Severe asphyctic pups showed an important neuronal loss in striatum and neocortex that was reduced by hypothermia. NADPH-d(+) neurons with reactive processes were found in the lateral zone of the striatum and neocortex in asphyctic pups. Controls and hypothermic striatum showed rounded cells without reactive process, while no cells were stained in cortex. There was also an increase in NADPH-d activity in endothelial cells in severe asphyctic pups in striatum and neocortex vs control and hypothermically treated animals. Our data evidenced that an inappropriate activation of NOS in neuronal and endothelial cells induced by PA is related to neuronal injury. Hypothermia inhibits neuronal injury and may be a valuable neuroprotective agent.
Assuntos
Animais Recém-Nascidos/fisiologia , Asfixia Neonatal/enzimologia , Encéfalo/enzimologia , Hipotermia Induzida , NADPH Desidrogenase/metabolismo , Animais , Asfixia Neonatal/patologia , Asfixia Neonatal/prevenção & controle , Comportamento Animal , Encéfalo/patologia , Feminino , Histocitoquímica , Humanos , Processamento de Imagem Assistida por Computador , Recém-Nascido , Neocórtex/enzimologia , Neocórtex/patologia , Neostriado/enzimologia , Neostriado/patologia , Neurônios/enzimologia , Óxido Nítrico Sintase/metabolismo , Gravidez , RatosRESUMO
Nitric oxide (NO) is known to be involved in the neuropathological mechanisms triggered by excitatory aminoacids. NO(+) neurons in the brain may be detected histochemically by nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemical technique, as the latter readily labels NO synthase in the central nervous system (CNS). NADPH-d stained striatal and cortical sections were studied in 6-month-old male Sprague-Dawley rats exposed to perinatal asphyxia (PA) at 37 degrees C, as well as in animals subjected to PA plus hypothermia treatment at 15 degrees C. Quantitative image analysis was performed to compare the staining pattern in the various groups. NADPH-d(+) neurons in striatum and cortex from subsevere and severe asphyctic animals showed a significant increase in soma size and in dendritic processes versus controls and hypothermia-treated rats. These findings indicate that chronic NO changes are involved in postischemic striatal and cortical alterations induced by PA that may be prevented by hypothermia.
Assuntos
Asfixia Neonatal/enzimologia , Córtex Cerebral/metabolismo , Hipotermia Induzida , NADPH Desidrogenase/metabolismo , Neostriado/metabolismo , Neurônios/enzimologia , Animais , Asfixia Neonatal/terapia , Benzoxazinas , Córtex Cerebral/citologia , Corantes , Feminino , Histocitoquímica , Humanos , Processamento de Imagem Assistida por Computador , Recém-Nascido , Masculino , Neostriado/citologia , Oxazinas , Gravidez , Ratos , Ratos Sprague-DawleyRESUMO
Striatal and cortical neurons containing nitric oxide synthase (NOS) were studied in adult rats subjected to different periods of perinatal asphyxia (PA) using immunohistochemistry at both light microscopy (LM) and electron microscopy (EM). Another group was subjected to PA + hypothermia to study its neuroprotective effect. Quantitative image analysis was performed on the striatum and neocortex in order to count the number of immunoreactive neurons and to compare the pattern of staining between the different groups. Six-month-old rats that suffered subsevere and severe PA demonstrated, at LM, cytomegaly of the striatal and neocortical neurons containing NOS. Control and hypothermic neurons were more weakly immunostained than PA neurons. Subsevere and severe asphyctic rats showed an important neuronal loss that was reduced by hypothermic treatment. The PA group disclosed, at EM, dense electronic bodies distributed in terminals surrounding synaptic vesicles and in dendrites. Non-NOS-containing neurons showed signs of degeneration, such as dark cytoplasm and shrunken nuclei. Surrounding the blood vessels, we observed a clear edema. The immunolabeling in hypothermic rats resembled that observed in controls. These data suggest that subsevere and severe PA induces chronic changes in the neuronal content of NOS in the striatum and neocortex. Degeneration observed in neurons surrounding cytomegalic NOS-containing cells may be due to the excess of NO in their environment. Moreover, the chronic alterations produced by PA seem to be prevented by hypothermia.
Assuntos
Asfixia Neonatal/enzimologia , Asfixia Neonatal/terapia , Encéfalo/enzimologia , Encéfalo/patologia , Hipotermia Induzida , Óxido Nítrico Sintase/metabolismo , Animais , Asfixia Neonatal/patologia , Benzoxazinas , Encéfalo/ultraestrutura , Corantes , Feminino , Humanos , Imuno-Histoquímica , Recém-Nascido , Microscopia Eletrônica , Neurônios/enzimologia , Neurônios/ultraestrutura , Oxazinas , Gravidez , Ratos , Ratos Sprague-DawleyRESUMO
Neuropathological mechanisms triggered by excitatory aminoacids are known to involve nitric oxide (NO). Neurons containing NO are histochemically reactive to nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), which labels NO synthase in CNS. Sprague-Dawley male rats subjected to perinatal asphyxia (PA) at 37 degrees C, and PA plus 15 degrees C hypothermia were evaluated when 6 months old by NADPH-d histochemical reaction. Computarized image analysis was used for quantification of stained sections. NADPH-d neurons in striatum from subsevere and severe PA showed a significant increment in soma size and dendritic process length versus control and hypothermic treated rats. Post-ischemic damage neurons are therefore involved in NO changes induced by PA that may be prevented by hypothermia treatment.
Assuntos
Asfixia Neonatal/metabolismo , Corpo Estriado/metabolismo , NADPH Desidrogenase/metabolismo , Neurônios/metabolismo , Óxido Nítrico/metabolismo , Análise de Variância , Animais , Animais Recém-Nascidos , Humanos , Hipotermia Induzida , Recém-Nascido , Masculino , Ratos , Ratos Sprague-Dawley , Fatores de TempoRESUMO
The aim of this study was to characterize the cytoskeletal intermediate filaments, glial fibrillary acidic protein (GFAP), and vimentin in normal and lead treated rats, and to compare the astroglial response in the cerebellum and the hippocampus -two regions with great susceptibility to the toxic effects of lead. Experiments combined light and electron microscopy immunohistochemistry using antibodies to GFAP and to vimentin, and conventional transmission electron microscopy techniques. Chronic lead administration was provided through the drinking water (1 g% lead acetate solution) and started when pups were 7 days old through the mother's milk. Following weaning lead intoxicated offspring were continuously exposed during 9 months, and sacrificed, with their corresponding controls, by perfusion-fixation at 30, 60, 75, 90, 180 and 270 days of lead exposure. After 60 and 90 days of treatment, hypertrophic astrocytes were observed in the cerebellum and hippocampus. Additionally, in the same time-period more GFAP immunolabelled astrocytes were detected in the cerebellum but not in the hippocampus. These qualitative observations were confirmed by computerized image analysis quantification. This effect was transient, even though the lead treatment was prolonged for 9 months and the blood-lead levels remained high after 30 days of the lead-exposure. After 90 days of lead administration, hypertrophic astrocytes started to decline and a gradual increment in the number of dense bodies, lipofuscin-like, was evidenced in astrocytes, neurons, pericytes and microglial cells. The data suggest that chronic lead exposure induces an astrocytic reaction as a result of a direct action of lead on astroglial cells or as a response to underlying neural damage.
Assuntos
Astrócitos/patologia , Cerebelo/efeitos dos fármacos , Proteína Glial Fibrilar Ácida/análise , Hipocampo/efeitos dos fármacos , Intoxicação por Chumbo/patologia , Vimentina/análise , Animais , Astrócitos/química , Divisão Celular/efeitos dos fármacos , Cerebelo/química , Cerebelo/patologia , Feminino , Hipocampo/química , Hipocampo/patologia , Hipertrofia , Processamento de Imagem Assistida por Computador , Técnicas Imunoenzimáticas , Intoxicação por Chumbo/metabolismo , Masculino , Microscopia Eletrônica , Ratos , Ratos WistarRESUMO
The effect of chronic lead exposure in neonatal rats was examined, particularly on hippocampal local circuit neurons (LCN). Chronic lead was administered to rat pups in the first week of life through the mother milk. During the lactation period the mother only drank water containing 1 g% lead acetate (w/v) -subclinical dose. Following weaning, the young rats were treated for 3 months with the same concentration of lead-adulterated water. Electron microscopy studies were made at 30, 45, 60, 75 and 90 days of age. After 2 months of lead exposure, irregular outline and dark-condensed cytoplasm in non-pyramidal neurons, in basket and in granule cells were found. Within the neuropil, some beaded and smooth dendrites, as well as some axons and synaptic terminals, appeared condensed and darker than the surrounding elements. Coincident with the LCN alterations, astrocytes showed an increment of glial filaments. These results suggested that the functional activity of LCN could be modified by the observed changes. Such ultrastructural modifications could be a morphological substrate for the subtle neuropsychological deficits of rats chronically exposed to relatively low concentration of lead.
Assuntos
Hipocampo/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Compostos Organometálicos/toxicidade , Animais , Animais Recém-Nascidos , Comportamento Animal/efeitos dos fármacos , Feminino , Hipocampo/patologia , Chumbo/sangue , Masculino , Microscopia Eletrônica , Neurônios/ultraestrutura , Gravidez , Ratos , Ratos WistarRESUMO
The present study was performed in order to follow the response of astroglial cells in the rat hippocampus to chronic low-level lead exposure. The experiments combined immunohistochemistry using anti-glial fibrillary acidic protein (GFAP) antibody and conventional transmission electron microscopy (EM). Chronic administration with drinking water [1 g% w/v (subclinical dose) of lead acetate dissolved in distilled water] was started through the mother's milk when pups were 7 days old. Following weaning, experimental offspring were treated for 3 months with the same concentration of adulterated water. The group of intoxicated animals and their controls were sacrificed by perfusion-fixation at 30, 60, and 90 days of exposure. After 60 days of lead treatment, staining of GFAP-positive cells demonstrated an astroglial transformation from the quiescent to the reactive state, characterized by an increase in GFAP. In control rats no changes in GFAP immunostaining were observed. The intensity of the astroglial response was enhanced after 90 days of lead intoxication, showing an increment of GFAP immunoreactivity. Quantification of these changes was made by computerized image analysis, confirming that the sectional areas of the astroglia in lead-exposed animals were larger than those in controls. These results are consistent with the ultrastructural alterations. Simultaneously with the increment in gliofilaments, intranuclear inclusions were seen in some astrocytes. The mechanisms by which lead affects astrocytes are unknown. Probably the astroglial changes induced by lead intoxication produce microenvironmental modifications that may disturb the neuronal function.
Assuntos
Astrócitos/efeitos dos fármacos , Hipocampo/citologia , Chumbo/toxicidade , Animais , Peso Corporal/efeitos dos fármacos , Feminino , Proteína Glial Fibrilar Ácida/imunologia , Hipocampo/efeitos dos fármacos , Processamento de Imagem Assistida por Computador , Imuno-Histoquímica , Chumbo/sangue , Microscopia Eletrônica , Gravidez , Ratos , Ratos EndogâmicosRESUMO
The effect of chronic lead exposure in neonatal rats was examined, particularly on hippocampal local circuit neurons (LCN). Chronic lead was administered to rat pups in the first week of life through the mother milk. During the lactation period the mother only drank water containing 1 g
lead acetate (w/v) -subclinical dose. Following weaning, the young rats were treated for 3 months with the same concentration of lead-adulterated water. Electron microscopy studies were made at 30, 45, 60, 75 and 90 days of age. After 2 months of lead exposure, irregular outline and dark-condensed cytoplasm in non-pyramidal neurons, in basket and in granule cells were found. Within the neuropil, some beaded and smooth dendrites, as well as some axons and synaptic terminals, appeared condensed and darker than the surrounding elements. Coincident with the LCN alterations, astrocytes showed an increment of glial filaments. These results suggested that the functional activity of LCN could be modified by the observed changes. Such ultrastructural modifications could be a morphological substrate for the subtle neuropsychological deficits of rats chronically exposed to relatively low concentration of lead.