RESUMO
Zinc finger protein 191, ZNF24 and Zfp191 in both humans and mice belong to the SCAN domain subfamily of Krüppel-like zinc finger transcription factors. Previous studies have suggested that Zfp191 is a pleiotropic factor involved in embryonic development, hematopoiesis and tumorigenesis. However, little is known about its target genes or its role in other physiological and pathological processes. We have identified the putative target genes of Zfp191, using an in silico genome-wide scan. Three hundred and fifty-five putative target genes were identified, which were enriched into the pathways of immune response according to the pathway analysis. These targets indicated that Zfp191 may function as a mediator of the immune response. This was verified in mice heterozygous for Zfp191 (Zfp191(+/-)) using a lipopolysaccharide (LPS)-induced endotoxic shock model. After LPS injection, Zfp191(+/-) mice produced significantly less IL-1ß and IL-6 compared to wild-type mice and were resistant to LPS-induced endotoxic shock. The loss of Zfp191 may suppress systemic inflammation by reducing these cytokine levels during LPS-induced endotoxic shock.
Assuntos
Proteínas de Transporte/genética , Resistência à Doença/genética , Resistência à Doença/imunologia , Técnicas de Inativação de Genes , Heterozigoto , Choque Séptico/genética , Choque Séptico/imunologia , Animais , Sequência de Bases , Sítios de Ligação , Biologia Computacional , Regulação da Expressão Gênica , Interleucina-1beta/sangue , Interleucina-6/sangue , Lipopolissacarídeos , Camundongos , Camundongos Knockout , Dados de Sequência Molecular , Especificidade de Órgãos , Ligação Proteica , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Choque Séptico/sangue , Choque Séptico/patologia , Análise de SobrevidaRESUMO
We describe a case of human T-lymphotropic virus type I associated myelopathy in a 50-year old woman in Nigeria. The patient presented with progressive loss of tone to the two lower limbs and later inability to walk. The HTLV-I antibody presence in the plasma collected from the patient was repeatedly detected by enzyme immunoassays (Abbott HTLV-I EIA and Coulter SELECT-HTLV I/II) and confirmed by Western blot technique. In addition, HTLV-I DNA was amplified from the genomic DNA isolated from the peripheral blood mononuclear cells of the patient by the polymerase chain reaction technique. This finding is significant being the first report of association of HTLV-I with myelopathy in Nigeria.