RESUMO
AIM: The aim of this study was to evaluate the IFI16 and IFN-α/ß receptors expression during the genesis and development of experimental apical periodontitis (AP) in mice teeth. METHODOLOGY: Apical periodontitis was induced in the lower first molars of 40 C57BL/6 mice. They were divided according to the experimental periods 2, 7, 14, 21 and 42 days (n = 8 per group). Five animals were used as a control group (without AP). Specimens were submitted to histological processing for description of the inflammatory process, immunostaining for the presence/absence and localization of IFI16 and IFN-α/ß receptors (qualitative and semi-quantitative analysis) and tartrate-resistant acid phosphatase (TRAP) histoenzimology. RESULTS: The results showed a gradual development of AP over the experimental times. The expression of IFI16 was noticeably more exacerbated in the experimental early period (day 2) whilst the lowest expression was observed in the control group (p = .02). For IFN-α/ß receptors, a higher intensity staining was observed 42 days after AP induction, that was statistically different from the control group (p = .02). In addition, the number of TRAP-positive cells was higher on the later periods (days 21 and 42; p < .001). CONCLUSION: IFI16 protein expression was highest during the early periods after AP induction in mice teeth, whilst IFN-α/ß receptor expression was highest after AP became established.
Assuntos
Interferon gama , Proteínas Nucleares/metabolismo , Periodontite Periapical , Fosfoproteínas/metabolismo , Receptor de Interferon alfa e beta/metabolismo , Animais , Camundongos , Camundongos Endogâmicos C57BL , Dente Molar/patologia , Osteoclastos/metabolismo , Periodontite Periapical/patologiaRESUMO
BACKGROUND/AIMS: Tooth avulsion consists of the complete displacement of a tooth from the alveolar socket. When immediate replantation is not possible, the avulsed tooth should be kept in a storage medium capable of maintaining the viability of periodontal ligament (PDL) cells on the root surface. However, there is no consensus on the best storage medium able to prevent sequels such as ankylosis and tooth resorption. The aim of this study was to perform a systematic review to evaluate the in vivo effectiveness of different storage media for avulsed teeth. METHODS: Two reviewers performed a database search for studies published between January 1950 and December 2015 which were indexed in the PubMed, Scopus, Web of Science, and Bireme databases. An additional manual search was performed. Studies with animal models that evaluated tooth avulsion, storage media, and replantation were included. After full-text analysis of the potentially relevant studies, the selected studies were included in the systematic review. RESULTS: The database search found 157 distinct studies evaluating avulsed teeth storage media. However, only six studies met the selection criteria and were included in the review. There was a high variability in the study estimates for the parameters analyzed. When assessing the quality and level of evidence of each study, one study was rated as having a very low level of evidence, four studies had low levels of evidence, and one had a moderate level of evidence. CONCLUSION: As a result of data heterogeneity and limitations of the studies, there was insufficient evidence to determine the most effective storage medium for avulsed teeth.
Assuntos
Modelos Animais , Soluções para Preservação de Órgãos/farmacologia , Manejo de Espécimes/métodos , Avulsão Dentária , AnimaisRESUMO
This study evaluated physiochemical proprieties of a calcium hydroxide-based paste (Calen®) combined with a zinc oxide cement at different ratios (1:0.5, 1:0.65, 1:0.8 and 1:1). Materials were compared regarding setting time, pH variation, radiopacity, solubility, dimensional changes, flow and release of chemical elements. Data were analyzed statistically by ANOVA and Tukey's test (α=0.05). Longer setting time and higher dimensional changes and solubility values were exhibited by 1:0.65 and 1:0.5 ratios (p<0.05). The 1:0.5 and 1:0.65 ratios exhibited the highest pH values at all time points. All materials exhibited high radiopacity values. Significant differences were found only between 1:0.5 and 1:1 ratios for calcium and zinc release (p<0.05), whereas the amount of zirconium was similar among all groups (p>0.05). Considering the evaluated proprieties, combinations of Calen® paste with ZO at 1:0.5 and 1:0.65 ratios had the best results as root canal filling materials for use in primary teeth.
Assuntos
Materiais Restauradores do Canal Radicular/química , Dente Decíduo , Humanos , Concentração de Íons de Hidrogênio , Teste de Materiais , Solubilidade , Espectrometria por Raios XRESUMO
Abstract This study evaluated physiochemical proprieties of a calcium hydroxide-based paste (Calen(r)) combined with a zinc oxide cement at different ratios (1:0.5, 1:0.65, 1:0.8 and 1:1). Materials were compared regarding setting time, pH variation, radiopacity, solubility, dimensional changes, flow and release of chemical elements. Data were analyzed statistically by ANOVA and Tukey's test (α=0.05). Longer setting time and higher dimensional changes and solubility values were exhibited by 1:0.65 and 1:0.5 ratios (p<0.05). The 1:0.5 and 1:0.65 ratios exhibited the highest pH values at all time points. All materials exhibited high radiopacity values. Significant differences were found only between 1:0.5 and 1:1 ratios for calcium and zinc release (p<0.05), whereas the amount of zirconium was similar among all groups (p>0.05). Considering the evaluated proprieties, combinations of Calen(r) paste with ZO at 1:0.5 and 1:0.65 ratios had the best results as root canal filling materials for use in primary teeth.
Resumo O objetivo do presente estudo foi avaliar propriedades físico-químicas da pasta à base de hidróxido de cálcio (Calen(r)) combinado com diferentes proporções de óxido de zinco (OZ) (1:0,5 e 1:0,65, 1:0,8 e 1:1) (Calen(r) /OZ). Os materiais foram comparados quanto ao tempo de endurecimento, variação do pH, radiopacidade, solubilidade, alterações dimensionais, escoamento e liberação de elementos químicos. Os dados obtidos foram submetidos à análise de variância (nível de 5% de significância). Maior grau de endurecimento, valores de alterações dimensionais, e solubilidade foram encontrados para Calen(r) /OZ 1:0,65 e 1:0,5. Calen(r) /OZ 1:0,5 e 1:0,65, apresentaram os maiores valores de pH em todos os momentos. Todos os materiais apresentaram valores elevados de radiopacidade com diferenças significativas (p>.05). Foram encontradas diferenças significativas apenas entre Calen(r) /OZ de 1:0,5 e 1:1 na liberação de cálcio e zinco (p< 0,05), enquanto que a quantidade de zircônia foi semelhante entre todos os grupos analisados (p>.05). Em relação às propriedades avaliadas no presente estudo, as combinações de Calen(r) com óxido de zinco nas proporções 1:0,5 e 1:0,65 são mais adequadas como material obturador para os canais radiculares de dentes decíduos.
Assuntos
Humanos , Concentração de Íons de Hidrogênio , Materiais Restauradores do Canal Radicular/química , Dente Decíduo , Teste de Materiais , Solubilidade , Espectrometria por Raios XRESUMO
The aim of this study was to evaluate radiographically and histologically the pulpal and periapical response to self-adhesive (Rely X™ Unicem) and self-etching and self-curing (Multilink(®)) resin-based luting materials in deep cavities in dogs' teeth. Deep class V cavities (0.5-mm-thick dentin) were prepared in 60 canine premolars and the following materials were applied on cavity floor: Groups I/V-RelyX™ Unicem; Groups II/VI-Multilink(®); Groups III/VII-zinc phosphate cement (control) and; Groups IV/VIII-gutta-percha (control). Cavities were restored with silver amalgam. Animals were euthanized after 10 days (groups I-IV) and 90 days (groups V-VIII). Tooth/bone blocks were radiographed and processed for histopathological evaluation of pulp and periapical tissue response to the materials. All materials presented similar histopathological features and radiographic findings at both periods. The pulp tissue was intact. The apical and periapical regions and periodontal ligament thickness were normal. No inflammatory cells, resorption of mineralized tissue (dentin, cementum, and alveolar bone) or bacteria were observed. The lamina dura was intact and no areas of periapical bone rarefaction or internal/external root resorption were observed radiographically. It can be concluded that Rely X™ Unicem and Multilink(®) caused no adverse tissue reactions and may be indicated for cementation of indirect restorations in deep dentin cavities without pulp exposure.
Assuntos
Dente Pré-Molar/diagnóstico por imagem , Cárie Dentária/patologia , Preparo da Cavidade Dentária , Cimentos Dentários/efeitos adversos , Resinas Sintéticas/administração & dosagem , Resinas Sintéticas/efeitos adversos , Animais , Cães , RadiografiaRESUMO
This study compared the physicochemical properties and interfacial adaptation to canal walls of Endo-CPM-Sealer, Sealapex and Activ GP with the well-established AH Plus sealer. The following analyses were performed: radiopacity, pH variation and solubility using samples of each material and scanning electron microscopy of root-filled bovine incisors to evaluate the interfacial adaptation. Data were analyzed by the parametric and no-parametric tests (α=0.05). All materials were in accordance with the ANSI/ADA requirements for radiopacity. Endo-CPM-Sealer presented the lowest radiopacity values and AH Plus was the most radiopaque sealer (p=0.0001). Except for ActiV GP, which was acidic, all other sealers had basic chemical nature and released hydroxyl ions. Regarding solubility, all materials met the ANSI/ADA recommendations, with no statistically significant difference between the sealers (p=0.0834). AH Plus presented the best adaptation to canal walls in the middle (p=0.0023) and apical (p=0.0012) thirds, while the sealers Activ GP and Endo-CPM-Sealer had poor adaptation to the canal walls. All sealers, except for ActiV GP, were alkaline and all of them fulfilled the ANSI/ADA requirements for radiopacity and solubility. Regarding the interfacial adaptation, AH Plus was superior to the others considering the adaptation to the bovine root canal walls.
Assuntos
Adaptação Marginal Dentária , Cavidade Pulpar/ultraestrutura , Materiais Restauradores do Canal Radicular/química , Ácidos/química , Resinas Acrílicas/química , Álcalis/química , Animais , Hidróxido de Cálcio/química , Bovinos , Fenômenos Químicos , Meios de Contraste/química , Colagem Dentária/métodos , Resinas Epóxi/química , Concentração de Íons de Hidrogênio , Hidróxidos/química , Teste de Materiais , Microscopia Eletrônica de Varredura , Salicilatos/química , Solubilidade , Propriedades de SuperfícieRESUMO
This study compared the physicochemical properties and interfacial adaptation to canal walls of Endo-CPM-Sealer, Sealapex and Activ GP with the well-established AH Plus sealer. The following analyses were performed: radiopacity, pH variation and solubility using samples of each material and scanning electron microscopy of root-filled bovine incisors to evaluate the interfacial adaptation. Data were analyzed by the parametric and no-parametric tests (α=0.05). All materials were in accordance with the ANSI/ADA requirements for radiopacity. Endo-CPM-Sealer presented the lowest radiopacity values and AH Plus was the most radiopaque sealer (p=0.0001). Except for ActiV GP, which was acidic, all other sealers had basic chemical nature and released hydroxyl ions. Regarding solubility, all materials met the ANSI/ADA recommendations, with no statistically significant difference between the sealers (p=0.0834). AH Plus presented the best adaptation to canal walls in the middle (p=0.0023) and apical (p=0.0012) thirds, while the sealers Activ GP and Endo-CPM-Sealer had poor adaptation to the canal walls. All sealers, except for ActiV GP, were alkaline and all of them fulfilled the ANSI/ADA requirements for radiopacity and solubility. Regarding the interfacial adaptation, AH Plus was superior to the others considering the adaptation to the bovine root canal walls.
Este estudo comparou as propriedades físico-químicas e a adaptação interfacial às paredes do canal dos cimentos Endo-CPM-Sealer, Sealapex e Activ GP com o bem estabelecido cimento AH Plus. As seguintes análises foram realizadas: radiopacidade, variação de pH e de solubilidade utilizando amostras de cada material, e microscopia eletrônica de varredura utilizando incisivos bovinos obturados para avaliar a adaptação interfacial. Os dados foram analisados utilizando testes paramétricos e não-paramétricos (α=0,05). Todos os materiais estavam de acordo com os requerimentos da ANSI/ADA para radiopacidade, sendo que o Endo-CPM-Sealer apresentou os menores valores de radiopacidade e o AH Plus foi o cimento mais radiopaco (p=0,0001). Exceto o Activ GP, que foi ácido, todos os outros cimentos apresentaram natureza química básica e liberaram íons hidroxila. Com relação à solubilidade, todos os materiais estavam de acordo com as recomendações da ANSI /ADA, sem diferença significante entre os cimentos (p=0,0834). O AH Plus apresentou a melhor adaptação às paredes do canal nos terços médio (p=0,0023) e apical (p=0,0012), enquanto que os cimentos Activ GP e Endo-CPM-Sealer apresentaram uma pobre adaptação às paredes do canal. Em conclusão, todos os cimentos, exceto o Activ GP, foram alcalinos e todos preencheram os requerimentos da ANSI/ADA para radiopacidade e solubilidade. Com relação à adaptação interfacial, o AH Plus foi superior aos demais para adaptação às paredes do canal radicular de incisivos bovinos.
Assuntos
Animais , Feminino , Humanos , Camundongos , Inibidores da Angiogênese/farmacologia , Antineoplásicos/farmacologia , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/patologia , Dextranos/farmacologia , Inibidores do Crescimento/farmacologia , Células Tumorais Cultivadas/efeitos dos fármacos , Células Tumorais Cultivadas/patologia , Inibidores da Angiogênese/uso terapêutico , Antineoplásicos/uso terapêutico , Meios de Cultivo Condicionados/farmacologia , Relação Dose-Resposta a Droga , Dextranos/química , Dextranos/uso terapêutico , Endotélio Vascular/citologia , Endotélio Vascular/efeitos dos fármacos , Endotélio Vascular/fisiologia , Inibidores do Crescimento/uso terapêutico , Camundongos Endogâmicos BALB C , Camundongos Nus , Necrose , Fenilacetatos/farmacologia , Fenilacetatos/uso terapêutico , Ensaios Antitumorais Modelo de Xenoenxerto/estatística & dados numéricosRESUMO
The aim of this study was to evaluate the tissue compatibility of a silorane-based resin system (Filtek™ Silorane) and a methacrylate-based nanoparticle resin (Filtek™ Supreme XT) after implantation in the subcutaneous connective tissue of isogenic mice. One hundred and thirty five male isogenic BALB/c mice were randomly assigned to 12 experimental and 3 control groups, according to the implanted material and the experimental period of 7, 21 and 63 days. At the end of each period, the animals were killed and the tubes with the surrounding tissues were removed and processed for microscopic analysis. Samples were subjected to a descriptive and a semi-quantitative analyses using a 4-point scoring system (0-3) to evaluate the collagen fiber formation and inflammatory infiltrate. Data were statistically analyzed using the Kruskal Wallis test (α=0.05). The results showed that there was no significant difference between the experimental and control groups considering the three evaluation periods (p>0.05). The silorane-based and the methacrylate-based nanoparticle resins presented similar tissue response to that of the empty tube (control group) after subcutaneous implantation in isogenic mice.
Assuntos
Resinas Compostas/toxicidade , Siloxanas/toxicidade , Tela Subcutânea/efeitos dos fármacos , Animais , Resinas Compostas/química , Colágenos Fibrilares/biossíntese , Reação a Corpo Estranho , Implantes Experimentais , Masculino , Teste de Materiais , Metacrilatos/toxicidade , Camundongos , Camundongos Endogâmicos BALB C , Distribuição Aleatória , Resinas de SiloranoRESUMO
PURPOSE: The objective of this paper is to report the clinical case of a patient who presented a chronic apical periodontitis, arising from internal inflammatory resorption followed by pulp necrosis, and a long-term success of a root canal therapy using calcium hydroxide as root canal dressing. CASE DESCRIPTION: A 20-year-old male patient presented for routine dental treatment. By radiographic examination we noted an extensive radioluscent area, laterally to the permanent maxillary right lateral incisor, with possibility of communication with the lateral periodontium, suggestive of a chronic apical periodontitis. Due to external root resorption detection, we used a calcium hydroxide root canal dressing, changed every 15 days, for a period of 2 months. Root canal filling was performed using gutta-percha cones by lateral condensation technique Radiographic follow up held after 19 years of treatment indicated a periodontium in conditions of normality, with the presence of lamina dura. CONCLUSION: Calcium hydroxide is a suitable material to be used as root canal dressing in teeth with apical periodontitis. Long-term evaluation demonstrated the satisfactory clinical outcome following root canal treatment.
OBJETIVO: O objetivo deste trabalho é relatar o caso clínico de um paciente que apresentava lesão periapical crônica decorrente de necrose de pulpar e o sucesso a longo prazo de uma terapia endodôntica utilizando o hidróxido de cálcio como curativo de demora. DESCRIÇÃO DO CASO: O paciente, do gênero masculino, com 20 anos de idade, compareceu à Clínica Odontológica para tratamento de rotina. Por meio de exames radiográficos observamos uma área radioluscente extensa, lateralmente ao incisivo lateral superior direito, com possibilidade de comunicação com o periodonto lateral, sugestivo de lesão periapical. Devido à presença de reabsorção radicular externa, foi utilizado um curativo de demora à base de hidróxido de cálcio (Calen®), trocado a cada 15 dias, por um período de 2 meses. A obturação do canal radicular foi realizada com cones de guta-percha pela técnica de condensação lateral ativa. O acompanhamento radiográfico realizado após 19 anos do tratamento endodôntico indicou um periodonto em condições de normalidade, com integridade da lamina dura. CONCLUSÃO: O hidróxido de cálcio é um material adequado para ser usado como curativo de demora em dentes com lesão periapical, uma vez que a avaliação a longo prazo demonstrou resultados clínicos satisfatórios após o tratamento endodôntico.
Assuntos
Humanos , Masculino , Adulto Jovem , Hidróxido de Cálcio/uso terapêutico , Tecido Periapical/lesões , Tratamento do Canal RadicularRESUMO
The aim of this study was to evaluate the tissue compatibility of a silorane-based resin system (FiltekTM Silorane) and a methacrylate-based nanoparticle resin (FiltekTM Supreme XT) after implantation in the subcutaneous connective tissue of isogenic mice. One hundred and thirty five male isogenic BALB/c mice were randomly assigned to 12 experimental and 3 control groups, according to the implanted material and the experimental period of 7, 21 and 63 days. At the end of each period, the animals were killed and the tubes with the surrounding tissues were removed and processed for microscopic analysis. Samples were subjected to a descriptive and a semi-quantitative analyses using a 4-point scoring system (0-3) to evaluate the collagen fiber formation and inflammatory infiltrate. Data were statistically analyzed using the Kruskal Wallis test (?=0.05). The results showed that there was no significant difference between the experimental and control groups considering the three evaluation periods (p>0.05). The silorane-based and the methacrylate-based nanoparticle resins presented similar tissue response to that of the empty tube (control group) after subcutaneous implantation in isogenic mice.
O objetivo do presente estudo foi avaliar a compatibilidade tecidual de um sistema resinoso à base de silorane (FiltekTM Silorane) e de uma resina nanoparticulada à base de metacrilato (FiltekTM Supreme XT), após implantação no tecido conjuntivo subcutâneo de camundongos isogênicos. Um total de 135 camundongos isogênicos BALB/c machos foram randomicamente divididos em 12 grupos experimentais e em 3 grupos controles, de acordo com o material implantado e com o período experimental (7, 21 e 63 dias). Ao final de cada período, os animais foram mortos, sendo os tubos removidos com o tecido circundante e processados para análise microscópica. As lâminas foram submetidas a análise descritiva e análise semi-quantitativa empregando um sistema de escores de 4 pontos (0-3), a fim de avaliar a formação de fibras colágenas e o infiltrado inflamatório. Os dados obtidos foram submetidos à análise estatística por meio do teste de Kruskal Wallis (?=0,05). Os resultados mostraram que não houve diferença estatisticamente significante entre os grupos experimentais e controles, considerando os três períodos de avaliação (p>0,05). As resinas à base de silorane e à base de metacrilato apresentaram resposta tecidual semelhante à do tubo vazio (controle), após implantação no tecido conjuntivo de camundongos isogênicos.