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1.
Mem Inst Oswaldo Cruz ; 104(5): 736-44, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19820835

RESUMO

The purpose of this work was to acquire an overview of the infectious cycle of HAdV-41 in permissive HEK 293 cells and compare it to that observed with the prototype of the genus, Human adenovirus C HAdV-2. HEK 293 cells were infected with each virus separately and were harvested every 12 h for seven days. Infection kinetics were analysed using confocal and electronic microscopy. The results show that, when properly cultivated, HAdV-41 was not fastidious. It had a longer multiplication cycle, which resulted in the release of complete viral particles and viral stocks reached high titres. After 60 h of infection, the export of viral proteins from the infected cell to the extracellular milieu was observed, with a pattern similar to that previously described for HAdV-2 penton-base trafficking after 30 h of infection. HAdV-41 had a non-lytic cycle and the infection spread from the first infected cell to its neighbours. The release process of the viral particles is unknown. The results observed for HAdV-41 infection in HEK 293 cells show how different this virus is from the prototype HAdV-2 and provides information for the development of this vector for use in gene therapy.


Assuntos
Adenovírus Humanos/crescimento & desenvolvimento , Adenovírus Humanos/classificação , Adenovírus Humanos/patogenicidade , Adenovírus Humanos/ultraestrutura , Animais , Linhagem Celular/virologia , Células Clonais , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Microscopia Confocal , Microscopia Eletrônica , Coelhos , Fatores de Tempo
2.
Mem. Inst. Oswaldo Cruz ; 104(5): 736-744, Aug. 2009. ilus
Artigo em Inglês | LILACS | ID: lil-528083

RESUMO

The purpose of this work was to acquire an overview of the infectious cycle of HAdV-41 in permissive HEK 293 cells and compare it to that observed with the prototype of the genus, Human adenovirus C HAdV-2. HEK 293 cells were infected with each virus separately and were harvested every 12 h for seven days. Infection kinetics were analysed using confocal and electronic microscopy. The results show that, when properly cultivated, HAdV-41 was not fastidious. It had a longer multiplication cycle, which resulted in the release of complete viral particles and viral stocks reached high titres. After 60 h of infection, the export of viral proteins from the infected cell to the extracellular milieu was observed, with a pattern similar to that previously described for HAdV-2 penton-base trafficking after 30 h of infection. HAdV-41 had a non-lytic cycle and the infection spread from the first infected cell to its neighbours. The release process of the viral particles is unknown. The results observed for HAdV-41 infection in HEK 293 cells show how different this virus is from the prototype HAdV-2 and provides information for the development of this vector for use in gene therapy.


Assuntos
Animais , Humanos , Coelhos , Adenovírus Humanos/crescimento & desenvolvimento , Adenovírus Humanos/classificação , Adenovírus Humanos/patogenicidade , Adenovírus Humanos/ultraestrutura , Células Clonais , Linhagem Celular/virologia , Técnica Indireta de Fluorescência para Anticorpo , Microscopia Confocal , Microscopia Eletrônica , Fatores de Tempo
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