RESUMO

Leishmania major, the causative agent of zoonotic leishmaniasis, is restricted to Old World countries. Molecular and biochemical techniques have been used to identify some L. major-like isolated in South America including Brazil. Here, two L. major-like strains, one virulent (BH49) and one non-virulent (BH121), were subjected to suppression subtractive hybridization (SSH) technique in order to identify differentially expressed genes. SSH technique identified nine cDNA fragments exhibiting high homology to previously sequenced L. major genes. Five cDNAs (four specific for BH49 and one for BH121) were confirmed by RT-PCR. Among those differentially expressed subtracted genes, some were involved in physiological processes including metabolism, translation and destination of proteins, production of energy, virulence factors and unknown functions. Western-blot analysis confirmed a higher expression level of ß-1,3-galactosyl residues in L. major-like lipophosphoglycan (LPG). This molecular analysis opens the possibility for identification of potential virulence factors not only in different strains, but also in others species of Leishmania.

Assuntos

DNA de Protozoário/genética , Leishmania major/genética , Leishmaniose Cutânea/parasitologia , Animais , Cricetinae , DNA Complementar/genética , Galactosiltransferases/metabolismo , Glicoesfingolipídeos/metabolismo , Humanos , Leishmania major/patogenicidade , Macrófagos/parasitologia , Mesocricetus , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Doenças Negligenciadas/parasitologia , Proteínas de Protozoários/metabolismo , Homologia de Sequência do Ácido Nucleico , Técnicas de Hibridização Subtrativa , Virulência/genética