RESUMO

Sudden changes in horses' diet have been previously associated with gastrointestinal disease. This study evaluated the effects of a sudden change of diet composed exclusively of Coastcross hay (CHD) to a complete extruded diet (CED) on the fecal microbiome of horses. A completely randomized design with repeated measurements was used. The study started with eight adult horses randomly split into group A, fed with CHD, and group B, fed with CED. After 34 days of diet adaptation, the diets were abruptly changed between the groups. Fecal samples were collected at 0, 24, and 96 hours after the diet change, and the pH and microbiome analyses of the feces were subsequently evaluated. Changing from CHD to CED reduced the alpha diversity 24 hours after the alteration, with a decrease in the relative abundance of Firmicutes and an increase of Bacteroidetes. Fecal pH decreased and the relative abundance of Verrucomicrobia increased 96 hours after changing the diets. The community structure was also different after 96 hours of diet change. In contrast, 24 hours after changing from CED to CHD reduced fecal pH and abundance of Synergistetes. After 96 hours, there was an increase in the alpha diversity, and the abundance of the phylum Lentisphaerae. Group B showed no changes in the community structure when its diet was changed. Concluding, diet composition influenced the response of the equine fecal microbiome to sudden dietary changes.

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Background: Equine colic syndrome comprises numerous conditions associated with abdominal pain in horses. Impaction, a common cause of this manifestation, is strongly related to these animals' diet. Highly fibrous diets such as sugarcane can predispose horses to colic. The clinical condition can be worsened by fermentative processes, which lead to dysbiosis, circulatory disorders and even endotoxemia. The aim of this study was to report 4 cases of colic syndrome among 8 horses that underwent an experiment to adapt them to a sugarcane-based diet, and to correlate the animals' clinical conditions to the forage they ingested. Cases: Eight male castrated Mangalarga Marchador horses, between 5.5 and 7 years old, were subjected to an experiment to test the feasibility of sugarcane as forage. Four of these horses were taken to the Large Animal Veterinary Hospital (HVGA) of the Federal Rural University of Rio de Janeiro for treatment of abdominal signs of discomfort a few days after the exclusive consumption of sugarcane, in a proportion of 1.75% of live weight in dry matter. The animals' symptoms ranged from behavioral signs indicative of pain to changes in vital parameters and structure of the feces, as well as changes revealed by transrectal palpation. Three of the 4 cases presented impaction in the small colon, and 1 of the horses also presented impaction in the right dorsal colon and rostral displacement of the pelvic flexure, with accumulation of contents in the right ventral colon and sternal flexure. Two of the cases were treated medically, while the other 2 required surgical intervention. The clinical condition of all the patients evolved favorably and they were discharged between 2 and 18 days. Discussion: Colic originating in the digestive system is a syndrome strongly associated with management, especially with respect to confinement, nutrition, and parasite control. During the experiment, 4 of the 8 horses fed with sugarcane presented with colic syndrome. The low quality of sugarcane fiber is due to the high degree of lignification of the plant cell wall, which favors accumulation of ingesta. The poor digestibility and sweet taste of this roughage favor increased consumption. Furthermore, its high sucrose content, associated with an increased rate of passage in the small intestine, alters the intestinal microbiome, and hence, the fermentation byproducts and pH of the ingesta. High intestinal content, allied to longer retention times in the colon and activation of the renin-angiotensin-aldosterone system, promote greater dryness of the ingesta, predisposing the occurrence of impactions in the most distal portion of the large intestine. Intestinal distension and mesenteric traction caused by the accumulation of contents and gases trigger pain, which can worsen due to displacement of the large colon. Small colon impaction, which is easily identified by transrectal palpation, evolves gradually and its treatment, both clinical and surgical, tends to have a favorable prognosis. The need for alternative food sources for horses is a growing demand; however, sugarcane as an exclusive roughage has been shown to be unsafe for horses. The low quality of the fiber and the high sucrose content of this forage can alter the digestive physiology of horses through changes in the passage rate, microbiome and motility of digesta, predisposing them to intestinal dysfunction, ingesta compaction and displacement of the large colon.

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Abstract This study aimed to evaluate the effect of aflatoxin B1 (AFB1) on the digestibility of nutrients and the hematological profile of horses. The assay lasted 40 days, with 12 days for adaptation and 28 days for experimentation. In the experimental stage, the horses were distributed in a completely randomized design, including three treatment groups with four animals in each group. The treatments used included 0 µg/kg (control), 50 µg/kg, and 100 µg/kg of AFB1 added to a concentrate in the basal diet. The basal diet contained mycotoxins from naturally contaminated feed. A digestibility essay was carried out at the end of the experimental period through partial feces collection, with LIPE® as an indicator. Blood samples were collected once a week during the assay for hematological and biochemical evaluations. The results of the hematological and biochemical parameters were submitted to analysis of variance (ANOVA) and compared by the Tukey test at 5% significance. The aflatoxins in the diet increased the leukocyte count, especially that of mature neutrophils. Creatine kinase and alkaline phosphatase (P 0.05) activities were higher in horses fed more toxic diets. The digestibility of nutrients was unaffected by the level of mycotoxins in the diet (P > 0.05).

Resumo Objetivou-se com esta pesquisa avaliar o efeito da Aflatoxina B1 (AFB1) na digestibilidade dos nutrientes e no perfil hematológico de equinos. O ensaio durou 40 dias, sendo 12 dias para adaptação e 28 dias para experimentação. No período experimental, os cavalos foram distribuídos em delineamento inteiramente casualizado, com três tratamentos, com quatro animais cada. Os tratamentos utilizados foram 0 µg/kg (controle), 50 µg/kg e 100 µg/kg de AFB1 adicionados ao concentrado da dieta basal. A dieta basal continha alimentos naturalmente contaminados com micotoxinas. Um ensaio de digestibilidade foi realizado no final do período experimental, pelo método de coleta parcial de fezes utilizando o LIPE® como indicador. Amostras de sangue foram coletadas uma vez por semana, durante o ensaio para avaliações hematológicas e bioquímicas. Os resultados dos parâmetros hematológicos, bioquímicos e do ensaio de digestibilidade foram submetidos à análise de variância (ANOVA) e comparados pelo teste de Tukey a 5% de significância. As aflatoxinas da dieta aumentaram a contagem de leucócitos, principalmente os neutrófilos maduros. A creatina quinase e a fosfatase alcalina (P 0,05) apresentaram maior atividade nos equinos alimentados com dietas com maior toxicidade. A digestibilidade dos nutrientes não foi alterada pelos níveis de micotoxinas presentes nas dietas (P>0,05).

RESUMO

This study aimed to evaluate the effect of aflatoxin B1 (AFB1) on the digestibility of nutrients and the hematological profile of horses. The assay lasted 40 days, with 12 days for adaptation and 28 days for experimentation. In the experimental stage, the horses were distributed in a completely randomized design, including three treatment groups with four animals in each group. The treatments used included 0 µg/kg (control), 50 µg/kg, and 100 µg/kg of AFB1 added to a concentrate in the basal diet. The basal diet contained mycotoxins from naturally contaminated feed. A digestibility essay was carried out at the end of the experimental period through partial feces collection, with LIPE® as an indicator. Blood samples were collected once a week during the assay for hematological and biochemical evaluations. The results of the hematological and biochemical parameters were submitted to analysis of variance (ANOVA) and compared by the Tukey test at 5% significance. The aflatoxins in the diet increased the leukocyte count, especially that of mature neutrophils. Creatine kinase and alkaline phosphatase (P < 0.05) activities were higher in horses fed more toxic diets. The digestibility of nutrients was unaffected by the level of mycotoxins in the diet (P > 0.05).

Objetivou-se com esta pesquisa avaliar o efeito da Aflatoxina B1 (AFB1) na digestibilidade dos nutrientes e no perfil hematológico de equinos. O ensaio durou 40 dias, sendo 12 dias para adaptação e 28 dias para experimentação. No período experimental, os cavalos foram distribuídos em delineamento inteiramente casualizado, com três tratamentos, com quatro animais cada. Os tratamentos utilizados foram 0 µg/kg (controle), 50 µg/kg e 100 µg/kg de AFB1 adicionados ao concentrado da dieta basal. A dieta basal continha alimentos naturalmente contaminados com micotoxinas. Um ensaio de digestibilidade foi realizado no final do período experimental, pelo método de coleta parcial de fezes utilizando o LIPE® como indicador. Amostras de sangue foram coletadas uma vez por semana, durante o ensaio para avaliações hematológicas e bioquímicas. Os resultados dos parâmetros hematológicos, bioquímicos e do ensaio de digestibilidade foram submetidos à análise de variância (ANOVA) e comparados pelo teste de Tukey a 5% de significância. As aflatoxinas da dieta aumentaram a contagem de leucócitos, principalmente os neutrófilos maduros. A creatina quinase e a fosfatase alcalina (P<0,05) apresentaram maior atividade nos equinos alimentados com dietas com maior toxicidade. A digestibilidade dos nutrientes não foi alterada pelos níveis de micotoxinas presentes nas dietas (P>0,05).

Assuntos

RESUMO

Objetivou-se com esta pesquisa avaliar o efeito da Aflatoxina B1 (AFB1) na digestibilidade dos nutrientes e no perfil hematológico de equinos. O ensaio durou 40 dias, sendo 12 dias para adaptação e 28 dias para experimentação. No período experimental, os cavalos foram distribuídos em delineamento inteiramente casualizado, com três tratamentos, com quatro animais cada. Os tratamentos utilizados foram 0 µg/kg (controle), 50 µg/kg e 100 µg/kg de AFB1 adicionados ao concentrado da dieta basal. A dieta basal continha alimentos naturalmente contaminados com micotoxinas. Um ensaio de digestibilidade foi realizado no final do período experimental, pelo método de coleta parcial de fezes utilizando o LIPE® como indicador. Amostras de sangue foram coletadas uma vez por semana, durante o ensaio para avaliações hematológicas e bioquímicas. Os resultados dos parâmetros hematológicos, bioquímicos e do ensaio de digestibilidade foram submetidos à análise de variância (ANOVA) e comparados pelo teste de Tukey a 5% de significância. As aflatoxinas da dieta aumentaram a contagem de leucócitos, principalmente os neutrófilos maduros. A creatina quinase e a fosfatase alcalina (P0,05).

This study aimed to evaluate the effect of aflatoxin B1 (AFB1) on the digestibility of nutrients and the hematological profile of horses. The assay lasted 40 days, with 12 days for adaptation and 28 days for experimentation. In the experimental stage, the horses were distributed in a completely randomized design, including three treatment groups with four animals in each group. The treatments used included 0 µg/kg (control), 50 µg/kg, and 100 µg/kg of AFB1 added to a concentrate in the basal diet. The basal diet contained mycotoxins from naturally contaminated feed. A digestibility essay was carried out at the end of the experimental period through partial feces collection, with LIPE® as an indicator. Blood samples were collected once a week during the assay for hematological and biochemical evaluations. The results of the hematological and biochemical parameters were submitted to analysis of variance (ANOVA) and compared by the Tukey test at 5% significance. The aflatoxins in the diet increased the leukocyte count, especially that of mature neutrophils. Creatine kinase and alkaline phosphatase (P 0.05).

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This study evaluated the influence of coastcross hay substrate hydration and equine fecal inoculum dilution on the parameters of fermentation and microbiology in in vitro essays. A 2 × 2 factorial block design was used. The first factor was hydration of the coastcross hay substrate 12 h before incubation or at the time of incubation, and the second factor was the dilution of fecal inoculum with a nutrient solution in a weight: weight ratio of 1:1 or 1:3. Degradation of the dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF) were evaluated at 24, 48, and 72 h. Microorganisms were counted 24 h after inoculation. The ammoniacal nitrogen concentration (NH3-N), pH, and cumulative gas production were measured up to 72 h and adjusted by the non-linear Gompertz regression model. Hydration of substrate and time of incubation increased nutrient degradation of coastcross hay, as well as the final volume of gases and the concentration of Streptococcus spp. The 1:3 dilution increased the final pH and Streptococcus spp. concentration. The hydration of substrate did not have any effect on NH3-N, Lactobacillus spp., cellulolytic, and total anaerobic bacteria concentrations. In addition, no effect of hydration was observed on the fermentation rate and the maximum fermentation time on the model used. The fermentation profile of the grass substrate is not affected by dilution, and, therefore, horse feces can be used as a source of inoculum in in vitro fermentation trials. Hydration increases the gas volumes and the nutrient degradation of grass hay, renders the lag phase time insignificant and, therefore, can be irrelevant in terms of fermentation model settings.(AU)

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During dry seasons, equines are traditionally fed hay. However, if not prepared under favorable climatic conditions and if not properly stored, hay does not preserve its nutritional value. The aim of this research was to evaluate the chemical composition in Stylosanthes guianensis cv. Mineirão and Estilosantes Campo Grande hay varieties, used to feed equines over 225 days of storage. Treatments consisted of Stylosanthes guianensis cv. Mineirão and Estilosantes Campo Grande hay varieties, assessed at six moments of storage: 0, 45, 90, 135, 180, and 225 days. The experiment followed a completely randomized split-plot design. In the comparison between varieties, differences (P<0.05) were found for dry matter, acid detergent insoluble nitrogen, neutral detergent fiber, and lignin. The storage period altered (P<0.05) the concentrations of dry matter, calcium, and crude protein in both hay varieties. The beta-carotene content in the leguminous plants did not decline (P>0.05) over the 225 days of storage. The storage period had little impact on the nutritional quality of the assessed hays. The Stylosanthes guianensis cv. Mineirão and, mainly, Estilosantes Campo Grande hay varieties have potential to be used in equine diet.(AU)

Durante a época seca, equinos são tradicionalmente alimentados com feno, entretanto, se não forem preparados em condições climáticas favoráveis e bem estocados, não preservam seu valor nutricional. O objetivo desse trabalho foi avaliar a influência do período de estocagem na composição bromatológica e concentração de betacaroteno dos fenos de Stylosanthes guianenses cv. Mineirão e Estilosantes Campo Grande durante 225 dias de armazenamento. Os tratamentos consistiram dos fenos de Stylosanthes guianenses cv. Mineirão e Estilosantes Campo Grande, avaliados em seis tempos de armazenamento: 0, 45, 90, 135, 180 e 225 dias. O delineamento experimental foi inteiramente ao acaso com arranjo em parcelas subdivididas. No comparativo entre as duas variedades, houve diferença (p<0,05) no teor de matéria seca, fibra em detergente neutro, lignina e nitrogênio insolúvel em detergente ácido. O período de armazenamento alterou (p<0,05) o teor de matéria seca, cálcio e proteína bruta de ambas as variedades de feno. As concentrações de betacaroteno dos fenos de Estilosantes não declinaram (P>0,05) ao longo dos 225 dias de armazenamento. O período de armazenamento não afetou a qualidade nutricional dos fenos avaliados. As variedades dos fenos de Stylosanthes guianenses cv. Mineirão e, principalmente, Estilosantes Campo Grande têm potencial para serem usadas na dieta de equinos.(AU)

Assuntos

RESUMO

During dry seasons, equines are traditionally fed hay. However, if not prepared under favorable climatic conditions and if not properly stored, hay does not preserve its nutritional value. The aim of this research was to evaluate the chemical composition in Stylosanthes guianensis cv. Mineirão and Estilosantes Campo Grande hay varieties, used to feed equines over 225 days of storage. Treatments consisted of Stylosanthes guianensis cv. Mineirão and Estilosantes Campo Grande hay varieties, assessed at six moments of storage: 0, 45, 90, 135, 180, and 225 days. The experiment followed a completely randomized split-plot design. In the comparison between varieties, differences (P0.05) over the 225 days of storage. The storage period had little impact on the nutritional quality of the assessed hays. The Stylosanthes guianensis cv. Mineirão and, mainly, Estilosantes Campo Grande hay varieties have potential to be used in equine diet.

Durante a época seca, equinos são tradicionalmente alimentados com feno, entretanto, se não forem preparados em condições climáticas favoráveis e bem estocados, não preservam seu valor nutricional. O objetivo desse trabalho foi avaliar a influência do período de estocagem na composição bromatológica e concentração de betacaroteno dos fenos de Stylosanthes guianenses cv. Mineirão e Estilosantes Campo Grande durante 225 dias de armazenamento. Os tratamentos consistiram dos fenos de Stylosanthes guianenses cv. Mineirão e Estilosantes Campo Grande, avaliados em seis tempos de armazenamento: 0, 45, 90, 135, 180 e 225 dias. O delineamento experimental foi inteiramente ao acaso com arranjo em parcelas subdivididas. No comparativo entre as duas variedades, houve diferença (p0,05) ao longo dos 225 dias de armazenamento. O período de armazenamento não afetou a qualidade nutricional dos fenos avaliados. As variedades dos fenos de Stylosanthes guianenses cv. Mineirão e, principalmente, Estilosantes Campo Grande têm potencial para serem usadas na dieta de equinos.

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This study was carried out in order to verify the asymmetries and similarities in horse breeding in the Brazilian South region and Argentina, analyzing the productive, health and trade aspects. Data collection were performed with questionnaires in fieldwork (primary sources) and bibliographic searches and surveys (secondary sources). The sample consisted of horses breeders in Brazil, Rio Grande do Sul State, and in Argentina. The questionnaire was based on the Brazilian Study of Horse Agribusiness Complex, divided into: Production Aspects, Health Issues and trade aspects. Descriptive data analysis and relative frequencies were compared using the chi-square (χ2) test at 5% probability. It was observed that there was a similarity in several aspects related to the breeding and trade horse agribusiness, whereas in the health aspects there was asymmetry in certain aspects. In the Southern Brazil, 29.0% of the owners live in the breeding farm and 67.7% have another remunerated activity. There was a similarity in the endoparasites system control and an asymmetry in the ectoparasites control. There was a higher percentage of diseases control in Argentina, which may be related to technification and qualification of workforce. However, regarding the equine sanitary control, there was a similarity between the farms of Southern Brazil and Argentina. Despite of all asymmetries and similarities, there is a relevance of horse agribusiness on generating jobs and income source, as well as the cultural and economic contribution in both countries. Furthermore, there is a necessity of public policies from government authority to promote the horse industry.

O objetivo com este estudo foi observar as similaridades e asimetrias existentes na criação de cavalos no sul do Brasil e Argentina, a partir de análise dos aspectos produtivos, comerciais e da saúde dos equinos. A metodologia utilizada compreendeu levantamento de dados, através de aplicação de questionário a campo (fontes primárias) e pesquisas bibliográficas (fontes secundárias). A amostragem abrangeu criadores de equinos do Rio Grande do Sul e Argentina. O questionário foi elaborado baseado no Estudo do Complexo do Agronegócio Cavalo no Brasil, dividido em: Aspectos de Proodução, Sanitários e Comerciais. Foi realizada análise descritiva dos dados e as frequências relativas foram comparadas pelo teste Qui-quadrado (P<0,05). Observou-se similaridades nos aspectos relacionados à criação e comercialização, mas assimetria relacionada à saúde dos equinos. No sul do Brasil 29,0% dos proprietários vivem da criação de cavalos, enquanto 67,7% exercem outras atividades remuneradas. Observou-se similaridade no controle de endoparasitas e assimetria em relação à ectoparasitas. A alta percentagem de controle de doenças observada na Argentina pode estar relacionada à tecnificação e a mão de obra treinada. Contudo, observou-se similaridade entre as fazendas do sul do Brasil e Argentina em relação ao controle sanitário dos rebanhos de equinos. A respeito das diversas assimetrias e semelhanças observadas, destaca-se a importância do agronegócio cavalo na produção de emprego e renda, bem como a contribuição econômica e cultural para ambos os países. Além disso, há necessidade de políticas públicas para a promoção da indústria do cavalo.

Assuntos

RESUMO

This study was carried out in order to verify the asymmetries and similarities in horse breeding in the Brazilian South region and Argentina, analyzing the productive, health and trade aspects. Data collection were performed with questionnaires in fieldwork (primary sources) and bibliographic searches and surveys (secondary sources). The sample consisted of horses breeders in Brazil, Rio Grande do Sul State, and in Argentina. The questionnaire was based on the Brazilian Study of Horse Agribusiness Complex, divided into: Production Aspects, Health Issues and trade aspects. Descriptive data analysis and relative frequencies were compared using the chi-square (χ2) test at 5% probability. It was observed that there was a similarity in several aspects related to the breeding and trade horse agribusiness, whereas in the health aspects there was asymmetry in certain aspects. In the Southern Brazil, 29.0% of the owners live in the breeding farm and 67.7% have another remunerated activity. There was a similarity in the endoparasites system control and an asymmetry in the ectoparasites control. There was a higher percentage of diseases control in Argentina, which may be related to technification and qualification of workforce. However, regarding the equine sanitary control, there was a similarity between the farms of Southern Brazil and Argentina. Despite of all asymmetries and similarities, there is a relevance of horse agribusiness on generating jobs and income source, as well as the cultural and economic contribution in both countries. Furthermore, there is a necessity of public policies from government authority to promote the horse industry.(AU)

O objetivo com este estudo foi observar as similaridades e asimetrias existentes na criação de cavalos no sul do Brasil e Argentina, a partir de análise dos aspectos produtivos, comerciais e da saúde dos equinos. A metodologia utilizada compreendeu levantamento de dados, através de aplicação de questionário a campo (fontes primárias) e pesquisas bibliográficas (fontes secundárias). A amostragem abrangeu criadores de equinos do Rio Grande do Sul e Argentina. O questionário foi elaborado baseado no Estudo do Complexo do Agronegócio Cavalo no Brasil, dividido em: Aspectos de Proodução, Sanitários e Comerciais. Foi realizada análise descritiva dos dados e as frequências relativas foram comparadas pelo teste Qui-quadrado (P<0,05). Observou-se similaridades nos aspectos relacionados à criação e comercialização, mas assimetria relacionada à saúde dos equinos. No sul do Brasil 29,0% dos proprietários vivem da criação de cavalos, enquanto 67,7% exercem outras atividades remuneradas. Observou-se similaridade no controle de endoparasitas e assimetria em relação à ectoparasitas. A alta percentagem de controle de doenças observada na Argentina pode estar relacionada à tecnificação e a mão de obra treinada. Contudo, observou-se similaridade entre as fazendas do sul do Brasil e Argentina em relação ao controle sanitário dos rebanhos de equinos. A respeito das diversas assimetrias e semelhanças observadas, destaca-se a importância do agronegócio cavalo na produção de emprego e renda, bem como a contribuição econômica e cultural para ambos os países. Além disso, há necessidade de políticas públicas para a promoção da indústria do cavalo.(AU)

Assuntos