RESUMO
This study aimed to evaluate the effect of Mauritia flexuosa oil on the structural integrity of spermcryopreserved goats. four clinically healthy goats were used. thirty-two samples were taken, with dilution inTris-egg yolk-glycerol and diluent containing vegetable oil (Mauritia flexuoxa). After cryopreservation, thesamples were thawed and evaluated for sperm plasma membrane integrity parameters of acrosome andmitochondrial activity. The percentage of cells with mitochondrial activity and acrosome integrity were higher inthe control group (P 0.05). The addition of the seminal Mauritia flexuoxa thinner not kept mitochondrial activityparameters and plasma membrane integrity of sperm at levels similar to conventional thinner, but was able tomaintain the integrity of the sperm plasma membrane.
Assuntos
Masculino , Animais , Criopreservação/classificação , Criopreservação/veterinária , Magnoliopsida/classificação , Magnoliopsida/química , Ruminantes/embriologia , EspermatozoidesRESUMO
The aim of this study was to investigate the effects of Tris use supplemented with Mauritia Flexuoxa oilas diluent for goat semen cryopreservation. four goats, clinically healthy were used. The animals were fed dailywith roughage, concentrate and mineral salt will. Initially, we conducted a toxicity test total of 32 collections.After the toxicity test, the concentration was chosen that showed the best result. Soon after, there were 32 morecollections, which were diluted in Tris-egg yolk-glycerol (control group) or GC diluent containing vegetable oil(Mauritia flexuoxa) GB. Samples were cryopreserved with the aid of Tk3000® device. Subsequently sampleswere thawed and analyzed for motility and sperm morphology force. It was observed obtaining post thawmotility in the group plus oil burity which shows a possible alternative to replacement products derived fromanimal origin.
Assuntos
Masculino , Animais , Análise do Sêmen/métodos , Análise do Sêmen/veterinária , Criopreservação/veterinária , Magnoliopsida/efeitos adversos , Magnoliopsida/fisiologiaRESUMO
The aim of this study was to investigate the effects of Tris use supplemented with Mauritia Flexuoxa oilas diluent for goat semen cryopreservation. four goats, clinically healthy were used. The animals were fed dailywith roughage, concentrate and mineral salt will. Initially, we conducted a toxicity test total of 32 collections.After the toxicity test, the concentration was chosen that showed the best result. Soon after, there were 32 morecollections, which were diluted in Tris-egg yolk-glycerol (control group) or GC diluent containing vegetable oil(Mauritia flexuoxa) GB. Samples were cryopreserved with the aid of Tk3000® device. Subsequently sampleswere thawed and analyzed for motility and sperm morphology force. It was observed obtaining post thawmotility in the group plus oil burity which shows a possible alternative to replacement products derived fromanimal origin.(AU)
Assuntos
Animais , Masculino , Criopreservação/veterinária , Magnoliopsida/efeitos adversos , Magnoliopsida/fisiologia , Análise do Sêmen/métodos , Análise do Sêmen/veterináriaRESUMO
This study aimed to evaluate the effect of Mauritia flexuosa oil on the structural integrity of spermcryopreserved goats. four clinically healthy goats were used. thirty-two samples were taken, with dilution inTris-egg yolk-glycerol and diluent containing vegetable oil (Mauritia flexuoxa). After cryopreservation, thesamples were thawed and evaluated for sperm plasma membrane integrity parameters of acrosome andmitochondrial activity. The percentage of cells with mitochondrial activity and acrosome integrity were higher inthe control group (P <0.05). There was no difference in the percentage of cells with membrane integrity betweenthe groups (P > 0.05). The addition of the seminal Mauritia flexuoxa thinner not kept mitochondrial activityparameters and plasma membrane integrity of sperm at levels similar to conventional thinner, but was able tomaintain the integrity of the sperm plasma membrane.(AU)