RESUMO
The aim of this work was to relate the occurrence of bacterial illnesses in two wild birds raised under captive conditions in Rio Grande do Norte State, Brazil, focusing on their clinical and microbiological findings. Clinical exams were performed in both birds in order to collect data and to determine the clinical and pathological outcome. In bird [A1] was diagnosed an infection in the respiratory system, and in bird [A2] the diagnosis was pododermatitis. Samples of shedding were collected using a sterile swab to perform a bacterial culture in blood agar and McConkey agar. An antibiogram was performed as well. The bacterial culture revealed the growing of Proteus sp. and Proteus vulgares, respectively. The antibiogram performed with the samples of bird [A1] showed the resistance to the follow antibiotics: ampicillin, amoxicillin/clavulanic acid, aztreonam, cefepime, ceftazidime, ciprofloxacin, tetracycline, sulfamethoxazole/trimethoprim and gentamicin. In bird [A2], strain was resistant to ampicillin, amoxicillin/clavulanic acid, aztreonam, cefotoxin, chloramphenicol, tetraciclin, sulfamethoxazole/trimethoprim and gentamicina. Proteus spp. appears to be a potencial multiresistant pathogen and causes severe lesions and diseases for wild birds in captivity.
O objetivo deste trabalho é relatar a ocorrência de enfermidades bacterianas em duas aves exóticas criadas em cativeiro no Rio Grande do Norte, com enfoque nos achados clínicos e microbiológicos. Realizou-se o exame clínico nas aves para a coleta de dados e caracterização do quadro clínico-patológico, sendo diagnosticada na ave [A1] infecção no trato respiratório superior, e na ave [A2] pododermatite profunda. O material biológico das secreções foi colhido com o auxílio de swabs estéreis para a determinação do agente etiológico através de cultura bacteriana em ágar sangue e ágar MacConkey. Realizou-se também o teste de susceptibilidade a antimicrobianos. A cultura bacteriológica das aves [A1] e [A2] resultou em crescimento de Proteus sp. e Proteus vulgares, respectivamente. O antibiograma da ave [A1] demonstrou resistência aos antibióticos: ampicilina, amoxicilina/ácido clavulâmico, aztreonam, cefepime, ceftazidima, ciprofloxacina, tetraciclina, sulfametoxazol/trimetroprim e gentamicina. Na ave [A2] a cepa isolada apresentou resistência a ampicilina, amoxicilina/ácido clavulâmico, aztreonam, cefoxitina, cloranfenicol, tetraciclina, sulfametoxazol/trimetroprim e gentamicina. Proteus spp. pode ser considerado um patógeno multirresistente, causador de lesões graves e doenças em aves selvagens criadas em cativeiro.
RESUMO
The aim of this work was to relate the occurrence of bacterial illnesses in two wild birds raised under captive conditions in Rio Grande do Norte State, Brazil, focusing on their clinical and microbiological findings. Clinical exams were performed in both birds in order to collect data and to determine the clinical and pathological outcome. In bird [A1] was diagnosed an infection in the respiratory system, and in bird [A2] the diagnosis was pododermatitis. Samples of shedding were collected using a sterile swab to perform a bacterial culture in blood agar and McConkey agar. An antibiogram was performed as well. The bacterial culture revealed the growing of Proteus sp. and Proteus vulgares, respectively. The antibiogram performed with the samples of bird [A1] showed the resistance to the follow antibiotics: ampicillin, amoxicillin/clavulanic acid, aztreonam, cefepime, ceftazidime, ciprofloxacin, tetracycline, sulfamethoxazole/trimethoprim and gentamicin. In bird [A2], strain was resistant to ampicillin, amoxicillin/clavulanic acid, aztreonam, cefotoxin, chloramphenicol, tetraciclin, sulfamethoxazole/trimethoprim and gentamicina. Proteus spp. appears to be a potencial multiresistant pathogen and causes severe lesions and diseases for wild birds in captivity.(AU)
O objetivo deste trabalho é relatar a ocorrência de enfermidades bacterianas em duas aves exóticas criadas em cativeiro no Rio Grande do Norte, com enfoque nos achados clínicos e microbiológicos. Realizou-se o exame clínico nas aves para a coleta de dados e caracterização do quadro clínico-patológico, sendo diagnosticada na ave [A1] infecção no trato respiratório superior, e na ave [A2] pododermatite profunda. O material biológico das secreções foi colhido com o auxílio de swabs estéreis para a determinação do agente etiológico através de cultura bacteriana em ágar sangue e ágar MacConkey. Realizou-se também o teste de susceptibilidade a antimicrobianos. A cultura bacteriológica das aves [A1] e [A2] resultou em crescimento de Proteus sp. e Proteus vulgares, respectivamente. O antibiograma da ave [A1] demonstrou resistência aos antibióticos: ampicilina, amoxicilina/ácido clavulâmico, aztreonam, cefepime, ceftazidima, ciprofloxacina, tetraciclina, sulfametoxazol/trimetroprim e gentamicina. Na ave [A2] a cepa isolada apresentou resistência a ampicilina, amoxicilina/ácido clavulâmico, aztreonam, cefoxitina, cloranfenicol, tetraciclina, sulfametoxazol/trimetroprim e gentamicina. Proteus spp. pode ser considerado um patógeno multirresistente, causador de lesões graves e doenças em aves selvagens criadas em cativeiro.(AU)
Assuntos
Animais , Proteus/patogenicidade , Bacteriologia/instrumentação , /farmacologia , Noxas/análise , Aves/classificaçãoRESUMO
The aim of this work was to relate the occurrence of bacterial illnesses in two wild birds raised under captive conditions in Rio Grande do Norte State, Brazil, focusing on their clinical and microbiological findings. Clinical exams were performed in both birds in order to collect data and to determine the clinical and pathological outcome. In bird [A1] was diagnosed an infection in the respiratory system, and in bird [A2] the diagnosis was pododermatitis. Samples of shedding were collected using a sterile swab to perform a bacterial culture in blood agar and McConkey agar. An antibiogram was performed as well. The bacterial culture revealed the growing of Proteus sp. and Proteus vulgares, respectively. The antibiogram performed with the samples of bird [A1] showed the resistance to the follow antibiotics: ampicillin, amoxicillin/clavulanic acid, aztreonam, cefepime, ceftazidime, ciprofloxacin, tetracycline, sulfamethoxazole/trimethoprim and gentamicin. In bird [A2], strain was resistant to ampicillin, amoxicillin/clavulanic acid, aztreonam, cefotoxin, chloramphenicol, tetraciclin, sulfamethoxazole/trimethoprim and gentamicina. Proteus spp. appears to be a potencial multiresistant pathogen and causes severe lesions and diseases for wild birds in captivity.
O objetivo deste trabalho é relatar a ocorrência de enfermidades bacterianas em duas aves exóticas criadas em cativeiro no Rio Grande do Norte, com enfoque nos achados clínicos e microbiológicos. Realizou-se o exame clínico nas aves para a coleta de dados e caracterização do quadro clínico-patológico, sendo diagnosticada na ave [A1] infecção no trato respiratório superior, e na ave [A2] pododermatite profunda. O material biológico das secreções foi colhido com o auxílio de swabs estéreis para a determinação do agente etiológico através de cultura bacteriana em ágar sangue e ágar MacConkey. Realizou-se também o teste de susceptibilidade a antimicrobianos. A cultura bacteriológica das aves [A1] e [A2] resultou em crescimento de Proteus sp. e Proteus vulgares, respectivamente. O antibiograma da ave [A1] demonstrou resistência aos antibióticos: ampicilina, amoxicilina/ácido clavulâmico, aztreonam, cefepime, ceftazidima, ciprofloxacina, tetraciclina, sulfametoxazol/trimetroprim e gentamicina. Na ave [A2] a cepa isolada apresentou resistência a ampicilina, amoxicilina/ácido clavulâmico, aztreonam, cefoxitina, cloranfenicol, tetraciclina, sulfametoxazol/trimetroprim e gentamicina. Proteus spp. pode ser considerado um patógeno multirresistente, causador de lesões graves e doenças em aves selvagens criadas em cativeiro.
Assuntos
Animais , Bacteriologia/instrumentação , Noxas/análise , Proteus/patogenicidade , Aves/classificaçãoRESUMO
The aim of this work was to relate the occurrence of bacterial illnesses in two wild birds raised under captive conditions in Rio Grande do Norte State, Brazil, focusing on their clinical and microbiological findings. Clinical exams were performed in both birds in order to collect data and to determine the clinical and pathological outcome. In bird [A1] was diagnosed an infection in the respiratory system, and in bird [A2] the diagnosis was pododermatitis. Samples of shedding were collected using a sterile swab to perform a bacterial culture in blood agar and McConkey agar. An antibiogram was performed as well. The bacterial culture revealed the growing of Proteus sp. and Proteus vulgares, respectively. The antibiogram performed with the samples of bird [A1] showed the resistance to the follow antibiotics: ampicillin, amoxicillin/clavulanic acid, aztreonam, cefepime, ceftazidime, ciprofloxacin, tetracycline, sulfamethoxazole/trimethoprim and gentamicin. In bird [A2], strain was resistant to ampicillin, amoxicillin/clavulanic acid, aztreonam, cefotoxin, chloramphenicol, tetraciclin, sulfamethoxazole/trimethoprim and gentamicina. Proteus spp. appears to be a potencial multiresistant pathogen and causes severe lesions and diseases for wild birds in captivity.
O objetivo deste trabalho é relatar a ocorrência de enfermidades bacterianas em duas aves exóticas criadas em cativeiro no Rio Grande do Norte, com enfoque nos achados clínicos e microbiológicos. Realizou-se o exame clínico nas aves para a coleta de dados e caracterização do quadro clínico-patológico, sendo diagnosticada na ave [A1] infecção no trato respiratório superior, e na ave [A2] pododermatite profunda. O material biológico das secreções foi colhido com o auxílio de swabs estéreis para a determinação do agente etiológico através de cultura bacteriana em ágar sangue e ágar MacConkey. Realizou-se também o teste de susceptibilidade a antimicrobianos. A cultura bacteriológica das aves [A1] e [A2] resultou em crescimento de Proteus sp. e Proteus vulgares, respectivamente. O antibiograma da ave [A1] demonstrou resistência aos antibióticos: ampicilina, amoxicilina/ácido clavulâmico, aztreonam, cefepime, ceftazidima, ciprofloxacina, tetraciclina, sulfametoxazol/trimetroprim e gentamicina. Na ave [A2] a cepa isolada apresentou resistência a ampicilina, amoxicilina/ácido clavulâmico, aztreonam, cefoxitina, cloranfenicol, tetraciclina, sulfametoxazol/trimetroprim e gentamicina. Proteus spp. pode ser considerado um patógeno multirresistente, causador de lesões graves e doenças em aves selvagens criadas em cativeiro.
RESUMO
The objectives of the present study were the subtyping of Campylobacter jejuni subsp. jejuni strains obtained from humans and different animal species using PCR-RFLP, and the detection, by means of the same technique, of strains related to serotype PEN O19:LIO 7, the main C. jejuni serotype linked to Guillain-Barré Syndrome (GBS). Seventy C. jejuni strains isolated from human feces (n=33), primates (n=15), dogs (n=5), swine (n=2), bovines (n=1), abortion material from goats (n=2) and poultry carcasses (n=12), all collected in the state of São Paulo, were subtyped by means of PCR-RFLP of fla A gene, using restriction endonucleases Hae III, Afa I and Mbo I. Seven subtypes were observed when using the enzyme Hae III; eight when using Mbo I; and seven when using Afa I. The combination of the three endonucleases led to 16 fla-RFLP subtypes, from which ten subtypes shared strains of human and animal origin. From these, seven subtypes were observed in human and broiler strains. In eight subtypes, the other animal species shared patterns with human strains. It was inferred that, besides broilers, swine, goats, dogs and primates may be sources of infection for human in São Paulo. PCR-RFLP is a highly discriminatory technique that may be applied to molecular epidemiology studies of samples from different origins. Besides, the study also enabled the detection of two human strains and two primate strains related to serotype PEN O19: LIO 7.
Assuntos
Humanos , Animais , Infecções por Campylobacter , Campylobacter jejuni/isolamento & purificação , Técnicas e Procedimentos Diagnósticos , Técnicas In Vitro , Polimorfismo de Fragmento de Restrição , Reação em Cadeia da Polimerase/métodos , Síndrome de Guillain-Barré/diagnóstico , Estudos Epidemiológicos , Métodos , Estudos de Amostragem , MétodosRESUMO
The objectives of the present study were the subtyping of Campylobacter jejuni subsp. jejuni strains obtained from humans and different animal species using PCR-RFLP, and the detection, by means of the same technique, of strains related to serotype PEN O19:LIO 7, the main C. jejuni serotype linked to Guillain-Barré Syndrome (GBS). Seventy C. jejuni strains isolated from human feces (n=33), primates (n=15), dogs (n=5), swine (n=2), bovines (n=1), abortion material from goats (n=2) and poultry carcasses (n=12), all collected in the state of São Paulo, were subtyped by means of PCR-RFLP of fla A gene, using restriction endonucleases Hae III, Afa I and Mbo I. Seven subtypes were observed when using the enzyme Hae III; eight when using Mbo I; and seven when using Afa I. The combination of the three endonucleases led to 16 fla-RFLP subtypes, from which ten subtypes shared strains of human and animal origin. From these, seven subtypes were observed in human and broiler strains. In eight subtypes, the other animal species shared patterns with human strains. It was inferred that, besides broilers, swine, goats, dogs and primates may be sources of infection for human in São Paulo. PCR-RFLP is a highly discriminatory technique that may be applied to molecular epidemiology studies of samples from different origins. Besides, the study also enabled the detection of two human strains and two primate strains related to serotype PEN O19: LIO 7.
RESUMO
The objectives of the present study were the subtyping of Campylobacter jejuni subsp. jejuni strains obtained from humans and different animal species using PCR-RFLP, and the detection, by means of the same technique, of strains related to serotype PEN O19:LIO 7, the main C. jejuni serotype linked to Guillain-Barré Syndrome (GBS). Seventy C. jejuni strains isolated from human feces (n=33), primates (n=15), dogs (n=5), swine (n=2), bovines (n=1), abortion material from goats (n=2) and poultry carcasses (n=12), all collected in the state of São Paulo, were subtyped by means of PCR-RFLP of fla A gene, using restriction endonucleases Hae III, Afa I and Mbo I. Seven subtypes were observed when using the enzyme Hae III; eight when using Mbo I; and seven when using Afa I. The combination of the three endonucleases led to 16 fla-RFLP subtypes, from which ten subtypes shared strains of human and animal origin. From these, seven subtypes were observed in human and broiler strains. In eight subtypes, the other animal species shared patterns with human strains. It was inferred that, besides broilers, swine, goats, dogs and primates may be sources of infection for human in São Paulo. PCR-RFLP is a highly discriminatory technique that may be applied to molecular epidemiology studies of samples from different origins. Besides, the study also enabled the detection of two human strains and two primate strains related to serotype PEN O19: LIO 7.
RESUMO
C. chauvoei presence was detected by means of polymerase chain reaction (PCR) from supernatant of culture in cooked meat medium of liver, muscle and metatarsian bone marrow samples of seven calves with blackleg symptoms. The isolation under anaerobic conditions of one muscle sample revealed Clostridium perfringens in pure culture.
Foi detectada presença de Clostridium chauvoei pela reação de PCR a partir de cultivo em cooked meat medium de amostras de fígado, músculo e medula óssea metatarsiana de sete bezerros acometidos de carbúnculo sintomático. O isolamento de uma amostra de músculo sob condições anaeróbias revelou Clostridium perfringens em cultura pura.
Assuntos
Bovinos , Carbúnculo , Clostridium , Infecções por Clostridium , Técnicas In Vitro , Meios de Cultura , Métodos , Reação em Cadeia da Polimerase , Estudos de AmostragemRESUMO
C. chauvoei presence was detected by means of polymerase chain reaction (PCR) from supernatant of culture in cooked meat medium of liver, muscle and metatarsian bone marrow samples of seven calves with blackleg symptoms. The isolation under anaerobic conditions of one muscle sample revealed Clostridium perfringens in pure culture.
Foi detectada presença de Clostridium chauvoei pela reação de PCR a partir de cultivo em cooked meat medium de amostras de fígado, músculo e medula óssea metatarsiana de sete bezerros acometidos de carbúnculo sintomático. O isolamento de uma amostra de músculo sob condições anaeróbias revelou Clostridium perfringens em cultura pura.