RESUMO

Extreme weather events, such as severe droughts, pose a threat to the sustainability of beef cattle by limiting the growth and development of forage plants and reducing the available pasture for animals. Thus, the search for forage species that are more tolerant and adapted to soil water deficit conditions is an important strategy to improve food supply. In this study, we propose utilizing the mathematical concept of the Manhattan distance to assess the variations in the morphological variables of tropical forage grasses under water-limited conditions. This study aimed to select genotypes of tropical forage grasses under different water stress levels (moderate or severe) at this distance and the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS). Nine varieties from five species were examined. Forage grasses were grown in 12-L pots under three soil irrigation regimes [100% pot capacity-PC (well-irrigated control), 60% PC (moderate drought stress), and 25% PC (severe drought stress)] with four replicates. Drought stress treatments were applied for 25 days during the forage grass tillering and stalk elongation phases. After exposure to drought stress, the growth and morphological traits of forage plants were evaluated. The results show that the use of the Manhattan distance combined with TOPSIS helps in the genotypic selection of more stable tropical forage grass varieties when comparing plants exposed to moderate and severe drought conditions in relation to the nonstressful environment (control). The 'ADR 300', 'Pojuca', 'Marandu', and 'Xaraés' varieties show greater stability when grown in a greenhouse and subjected to water stress environments. The selected forage varieties can be used as parents in plant breeding programs, allowing us to obtain new drought-resistant genotypes.

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The genus Gossypium has important ethnobotanical and economic value for Amazonian Native Communities (A.N.C.). However, little research has been undertaken on the distribution and genetic diversity of cotton populations maintained in the Peruvian rainforest. This work aims to present the first report on the genetic diversity of Gossypium spp. populations in the A.N.C. of the province of La Convención, Cusco-Peru. The methodology was based on exploring, collecting, identifying, and characterizing the Gossypium populations present in the A.N.C. Twenty-six descriptors were evaluated (9 quantitative and 17 qualitative), and with this information, distribution, correlation, and principal component (PC) analyses were carried out. As a result, plants of two species [G. barbadense L. (44 samples) and G. hirsutum L. (19 samples)], one variety [G. barbadense var. brasiliensis (75 samples)], and three previously unidentified variations (9 samples) were identified. Altogether, 147 samples were collected. G. barbadense var. brasiliensis, which was always found in association with other economic crops within an altitude range of 338 to 1086 m, was the most predominant (51%), distributed in eleven A.N.C. and always in small plots (up to 2 ha). G. barbadense L. was cultivated between 397 and 1137 m of altitude in eight A.N.C. in plots of up to 3 ha in marginal lands. G. hirsutum L., with a smaller distribution (13%), was found between 334 and 497 m of altitude in only three communities; this species is cultivated in marginal areas throughout the year. The variability found for the first two PCs when considering the quantitative and qualitative descriptors was high (74.7%) and moderate (48.2%), respectively. When combining all the descriptors, the analysis showed that the first two PCs accounted for 51.8% of the total variability of the data. The PCs of the two types of data and their combination confirmed that the three populations found were grouped. The nine undefined samples were close to or intermediate between the described ones, showing that these samples may be the result of spontaneous crosses; as such, these samples need to be better evaluated with other tools for further definition. The information obtained shows that in the A.N.C. of Cusco-Peru, there is variability conserved by the inhabitants, who have been able to maintain and use these genotypes, even from their Amazonian indigenous ancestry, and the environment has been able to generate variability among the species, as will be highlighted in future works.

RESUMO

The search for soybean genotypes more adapted to abiotic stress conditions is essential to boost the development and yield of the crop in Brazil and worldwide. In this research, we propose a new approach using the concept of distance (or similarity) in a vector space that can quantify changes in the morphological traits of soybean seedlings exposed to stressful environments. Thus, this study was conducted to select soybean genotypes exposed to stressful environments (saline or drought) using similarity based on Manhattan distance and the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) method. TOPSIS is a multi-criteria decision method for selecting the best alternative using the concept of distance. The use of TOPSIS is essential because the genotypes are not absolutely similar in both treatments. That is, just the distance measure is not enough to select the best genotype simultaneously in the two stress environments. Drought and saline stresses were induced by exposing seeds of 70 soybean genotypes to -0.20 MPa iso-osmotic solutions with polyethylene glycol-PEG 6000 (119.6 g L-1) or NaCl (2.36 g L-1) for 14 days at 25 °C. The germination rate, seedling length, and seedling dry matter were measured. We showed here how the genotypic stability of soybean plants could be quantified by TOPSIS when comparing drought and salinity conditions to a non-stressful environment (control) and how this method can be employed under different conditions. Based on the TOPSIS method, we can select the best soybean genotypes for environments with multiple abiotic stresses. Among the 70 tested soybean genotypes, RK 6813 RR, ST 777 IPRO, RK 7214 IPRO, TMG 2165 IPRO, 5G 830 RR, 98R35 IPRO, 98R31 IPRO, RK 8317 IPRO, CG 7464 RR, and LG 60177 IPRO are the 10 most stable genotypes under drought and saline stress conditions. Owing to high stability and gains with selection verified for these genotypes under salinity and drought conditions, they can be used as genitors in breeding programs to obtain offspring with higher resistance to antibiotic stresses.

RESUMO

Periods of soil water stress have been recurrent in the Cerrado region and have become a growing concern for Brazilian tropical pasture areas. Thus, the search for forage grasses more tolerant to water stress has intensified recently in order to promote more sustainable livestock. In a greenhouse experiment, the degree of water stress tolerance of nine tropical forage grass cultivars was studied under different soil water regimes. The investigation followed a 9 × 3 factorial design in four randomized blocks. Nine cultivars from five species of perennial forage grasses were tested: Urochloa brizantha ('BRS Piatã', 'Marandu', and 'Xaraés'), Panicum maximum ('Aruana', 'Mombaça', and 'Tanzânia'), Pennisetum glaucum ('ADR 300'), Urochloa ruziziensis ('Comum'), and Paspalum atratum ('Pojuca'). These cultivars were grown in pots under three soil water regimes (high soil water regime-HSW (non-stressful condition), middle soil water regime-MSW (moderate water stress), and low soil water regime-LSW (severe water stress)). Plants were exposed to soil water stress for 25 days during the tillering and stalk elongation phases. Twelve tolerance indices, including tolerance index (TOL), mean production (MP), yield stability index (YSI), drought resistance index (DI), stress tolerance index (STI), geometric mean production (GMP), yield index (YI), modified stress tolerance (k1STI and k2STI), stress susceptibility percentage index (SSPI), abiotic tolerance index (ATI), and harmonic mean (HM), were calculated based on shoot biomass production under non-stressful (YP) and stressful (YS) conditions. Soil water stress decreased leaf area, plant height, tillering capacity, root volume, and shoot and root dry matter production in most cultivars, with varying degrees of reduction among tropical forage grasses. Based on shoot biomass production under controlled greenhouse conditions, the most water-stress-tolerant cultivars were P. maximum cv. Mombaça and cv. Tanzânia under the MSW regime and P. maximum cv. Aruana and cv. Mombaça under the LSW regime. P. maximum cv. Mombaça has greater adaptability and stability of shoot biomass production when grown under greenhouse conditions and subjected to soil water stress. Therefore, this forage grass should be tested under field conditions to confirm its forage production potential for cultivation in tropical regions with the occurrence of water stress. The MP, DI, STI, GMP, YI, k2STI, and HM tolerance indices were the most suitable for identifying forage grass cultivars with greater water stress tolerance and a high potential for shoot biomass production under LSW regime.

RESUMO

Excessive rainfall in the soybean preharvest period can make mechanized crop harvesting technically and economically unfeasible, causing 100% losses in soybean grain yield. An alternative to reduce the economic losses of farmers would be using unharvested soybean crop residues as a source of nitrogen (N) for the subsequent corn crop. However, a question that still needs to be understood is whether the amount of N released from unharvested soybean residues (straw and grains) is sufficient to meet all the nutritional demand for this nutrient in the off-season corn. Therefore, this study investigated the impact of unharvested soybean crop residue persistence on the yield response of off-season corn crop (Zea mays L.) to the application of N fertilizer rates when grown in tropical Cerrado soils of medium and high fertility. Four simple corn hybrids (SYN7G17 TL, 30F53VYHR, B2433PWU, and AG 8700 PRO3) were grown in soils of medium fertility and medium acidity level (UFMS 1) and high fertility and low acidity level (UFMS 2) and fertilized with five of N fertilizer rates (0, 40, 80, 120, and 160 kg ha-1 of N) applied at 30 days after emergence (DAE). Canonical correlation analysis (CCA) was used to investigate the interrelationships between the groups of independent (agricultural production areas, corn cultivars, and N application rates) and dependent (corn agronomic traits) variables. Crop residues remaining on the soil surface from soybeans not harvested and inoculated with Bradyrhizobium spp. can supply most of the nitrogen requirement of off-season corn grown in succession, especially in tropical soils of medium fertility. However, in high-fertility tropical soils, the maximum grain yield potential of off-season corn cultivars can be obtained with the application of mineral N fertilizer in supplement the amount of nitrogen released from unharvested soybean residues. Therefore, the N requirement depends on the corn cultivar and the agricultural production area. However, our results show that when off-season corn is grown on unharvested soybean residues, nitrogen fertilization in topdressing can be dispensed. The agricultural area with high fertility soil (UFMS 2) enhances the grain yield of the off-season corn crop. The corn cultivar AG 8700 PRO3 has a higher thousand-grain mass and high grain yield potential under Brazilian Cerrado conditions.

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This paper presents an innovative concept for the online application of Frequency Response Analysis (FRA). FRA is a well known technique that is applied to detect damage in electric machinery. As an offline technique, the machine under testing has to be removed from service-which may cause loss of production. Experimental adaptations of FRA to online operation are usually based on the use of passive high pass coupling-which, ideally, should provide attenuation to the grid voltage, and at the same time, allow the high frequency FRA signals to be injected at the machine. In practice, however, the passive coupling results in a trade-off between the required attenuation and the useful area obtained at the FRA spectra. This paper proposes the use of an active coupling system, based on power electronics, in order to cancel the grid voltage at the terminals of FRA equipment and allow its safe connection to an energized machine. The paper presents the basic concepts of FRA and the issue of online measurements. It also presents basic concepts about power electronics converters and the operating principles of the Modular Multilevel Converter, which enables the generation of an output voltage with low THD, which is important for tracking the grid voltage with minimum error.

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The rubber tree (Hevea brasiliensis Müell. Arg.) is a species of significant economic interest in the natural rubber industry in Brazil and the world. This species presents recalcitrance to rooting, and its cuttings are difficult to propagate. This study aimed to evaluate the effect of the pre-conditioning of rubber tree mini-cuttings with zinc on the improvement of the adventitious rooting of rootstocks. Mini-cuttings were standardized with 45 mm length and submitted to preconditioning by immersion of the mini-cutting base in solutions containing 0.00; 0.04; 0.08; 0.16; 0.32 and 0.64 mg L-1 of Zn, for 24 hours. The experimental design was randomized blocks with six treatments and four replicates of 10 mini-cuttings. The rubber tree mini-cuttings were placed in a fitotron-type growth chamber, at 25 °C, with 12-hour photoperiod, 5,000 K intensity, and 95% of relative air humidity, for 60 days. The survival rate, number of buds, percentage of mini-cuttings that had leaf abscission, the percentage of mini-cuttings with callogenesis in the root meristem, the percentage of rooted mini-cuttings, the number of primary roots and root length were evaluated. The highest values of survival rate, the number of buds, the number of primary roots, the percentage of mini-cuttings with callogenesis in the root meristem, the percentage of rooted mini-cuttings and root length were observed with 0.16 to 0.26 Mg L-1 of Zn. The use of zinc in the mini-cuttings of rubber tree reduces linearly the percentage of mini-cuttings that had leaf abscission and the formation of callogenesis in the root meristem.

A seringueira (Hevea brasiliensis Müell. Arg.) é uma espécie de importância econômica para a indústria da borracha natural do Brasil e do mundo. Esta espécie apresenta recalcitrância ao enraizamento e suas estacas são difíceis de se propagar. Este trabalho objetivou avaliar o efeito do pré-condicionamento de miniestacas de seringueira com zinco na melhoria do enraizamento adventício de porta-enxertos. As miniestacas foram padronizadas com 45 mm de comprimento e submetidas ao pré-condicionamento por imersão da miniestaca em soluções contendo 0.00; 0,04; 0,08; 0,16; 0,32 e 0,64 mg L-1 de Zn, por 24 horas. O delineamento experimental foi em blocos ao acaso, com seis tratamentos e quatro repetições de 10 miniestacas. As miniestacas de seringueira foram colocadas em câmara de crescimento tipo fitotron, a 25 °C, com fotoperíodo de 12 horas, intensidade de 5.000 K e umidade relativa do ar de 95% por 60 dias. Foram avaliadas a taxa de sobrevivência, o número de gemas, a porcentagem de miniestacas que apresentaram abscisão foliar, o percentual de miniestacas com calogênese no meristema radicular, o percentual de miniestacas enraizadas, o número de raízes primárias e o comprimento das raízes. Os maiores valores de taxa de sobrevivência, o número de gemas, o número de raízes primárias, a porcentagem de miniestacas com calogênese no meristema radicular, o percentual de miniestacas enraizadas e o comprimento radicular foram verificados com 0,16 a 0,26 Mg L-1 de Zn. O uso de zinco nas miniestacas de seringueira reduz linearmente a porcentagem de miniestacas que tiveram abscisão foliar e a formação de calogênese no meristema radicular

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The effects of intense cropping and potassium fertilization on potassium (K) dynamics and non-exchangeable K release from three lowland soils of Paraná State, Brazil, were investigated in this study. Samples of three lowland soils were fertilized or not with K and subjected to six successive croppings (soybeans, pearl millet, wheat, common beans, soybeans, and maize). The crops were grown in 8-L pots for 45 days, and at the end of the sixth cropping, the soil from each pot was sampled to the determination of soil K pools. The lowland soils differ in the ability to K supply to the plants in the short to medium term due to the wide range of origin material and the concentration of K in solution, exchangeable K, non-exchangeable K, and structural K. When the soils were not fertilized with K, the successive cropping resulted in continuous depletion process of non-exchangeable and exchangeable K; however, this depletion was less pronounced in soils with a higher potential buffer capacity of K. Non-exchangeable and exchangeable K concentrations were increased with the addition of K fertilizer, indicating the occurrence of K fixation in soil. The non-exchangeable K contribution to K nutrition of plants ranged from 44 to 69% in the treatments without the addition of K fertilizer, reporting the importance of non-exchangeable K pools in the supply of this nutrient to plants in agricultural production systems.

Os efeitos dos cultivos sucessivos e da adubação potássica na dinâmica de potássio (K) do solo e na mobilização do K não-trocável para às plantas em três solos de varzea do Estado do Paraná, Brasil, foram investigados neste estudo. Amostras dos três solos de várzea foram submetidas à adição ou não de fertilizante potássico e a seis cultivos sucessivos de plantas (soja, milheto, trigo, feijão, soja e milho). As culturas foram cultivadas em vasos de 8-L por 45 dias e, ao final do sexto cultivo, foram coletadas amostras de solos para a deteminação das diferentes formas de K do solo. Os solos de vázea diferenciaram-se na capacidade de suprir K às plantas a curto e médio prazo, devido à ampla variação do material de origem e dos teores de K na solução, K trocável, K não-trocável e K estrutural. Quando os solos não foram adubados com K, o cultivo sucessivo de plantas resultou em um processo contínuo de esgotamento das formas de K não-trocável e K trocável, sendo menos acentuada nos solos com maior poder tampão de potássio. Os teores de K não-trocável e K trocável aumentaram com a adição de fertilizantes potássicos, indicando a ocorrência de fixação de K pelo solo. A contribuição do K não-trocável para a nutrição das plantas durante os seis cultivos variou de 44 a 69% no tratamento sem adição de fertilizante potássico. Estes resultados reportam a importância das formas de K não-trocável para o suprimento deste nutriente às plantas nos sistemas de produção agrícolas.

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Um estudo foi conduzido com o objetivo de avaliar os efeitos da interação entre a inoculação da muda pré-brotada com Azospirillum brasilense e a adubação nitrogenada em cobertura no desenvolvimentodas plantas de cana-de-açúcar(Saccharum officinarum). Mudas pré-brotadas de cana-de-açúcar, oriundas de minirrebolos de 3,0cm de comprimento da variedade RB867515, foram transplantadas aos 25 dias após a brotação para vasos plásticos de 12 L. Os tratamentos foram dispostos em um delineamento de blocos casualizados, em esquema fatorial 2 × 5:dois tratamentos de inoculação [sem (controle) e com inoculação de Azospirillum brasilense] e cinco doses de adubação nitrogenada em cobertura (0, 30, 60, 90 e 120 mg dm–3de N), com quatro repetições. A inoculação com A. brasilense foi realizada no transplantio das mudas com o uso de 4 mL do inoculante AzoTotal®por muda, contendo as estirpes AbV5e AbV6. A adubação nitrogenada em cobertura foi realizada aos 20 dias após o transplantio das mudas na forma de ureia (45% de N).Os resultados reportaram que a aplicação de doses de N em coberturaresultou no incremento linear da altura de planta, diâmetro de colmo, número de folhas por planta, número de perfilhos e produção de matéria seca das plantas inoculadas com A. brasilense. Quando as plantas não foram inoculadas com A. brasilense, a adubação nitrogenada resultou apenas no aumento do número de folhas e de perfilhos por planta. O uso da inoculação de A. brasilenseem mudas de cana-de-açúcar pré-brotadas tem efeito benéfico no desenvolvimento das plantas somente quando associada a adubação nitrogenada em cobertura. As plantas de cana-de-açúcar não adubadas com fertilizante nitrogenado tem resposta negativa à inoculação com A. brasilense, indicando a ocorrência de possíveis efeitos fitotóxicos no desenvolvimento das plantas até os 60 dias após o transplantio.

A study was conducted with the objective of investigating the effects of the interaction between the inoculation of the pre-sprouted seedlings with Azospirillum brasilenseand the nitrogen fertilization in topdressing on the development of sugarcane plants(Saccharum officinarum). Pre-sprouted sugarcane seedlings, produced from 3.0 cm long mini-stems of the RB867515 variety, were transplanted 25 days after sprouting into 12 L plastic pots. The treatments were arranged in a randomized block design, in a 2 × 5 factorial scheme: two inoculation treatments [without (control) and inoculation with Azospirillum brasilense] and five application rates of N fertilizer in topdressing (0, 30, 60, 90 and 120 mg dm–3of N), with four repetitions. The inoculation with A. brasilense was carried out in transplanting the seedlings using 4 mL of the inoculum AzoTotal® per seedling, containing the strains AbV5 and AbV6. The nitrogen fertilization in topdressing was performed at 20 days after transplanting the seedlings as urea (45% of N). The results reported that the application of N fertilizer rates in topdressing resulted in a linear increase in plant height, stem diameter, number of leaves per plant, number of tillers and dry matter production of plants inoculated with A. brasilense. When the plants were not inoculated with A. brasilense, nitrogen fertilization resulted in an increase also in the number of leaves and tillers per plant. The use of A. brasilense inoculation in pre-sprouted sugarcane seedlings has a beneficial effect on the development of plants only when associated with nitrogen fertilization in topdressing. Sugarcane plants not fertilized with N have a negative response to inoculation with A. brasilense, indicating the occurrence of possible phytotoxic effects on plant development up to 60 days after transplanting.

Assuntos

RESUMO

Um estudo foi conduzido com o objetivo de avaliar os efeitos da interação entre a inoculação da muda pré-brotada com Azospirillum brasilense e a adubação nitrogenada em cobertura no desenvolvimentodas plantas de cana-de-açúcar(Saccharum officinarum). Mudas pré-brotadas de cana-de-açúcar, oriundas de minirrebolos de 3,0cm de comprimento da variedade RB867515, foram transplantadas aos 25 dias após a brotação para vasos plásticos de 12 L. Os tratamentos foram dispostos em um delineamento de blocos casualizados, em esquema fatorial 2 × 5:dois tratamentos de inoculação [sem (controle) e com inoculação de Azospirillum brasilense] e cinco doses de adubação nitrogenada em cobertura (0, 30, 60, 90 e 120 mg dm–3de N), com quatro repetições. A inoculação com A. brasilense foi realizada no transplantio das mudas com o uso de 4 mL do inoculante AzoTotal®por muda, contendo as estirpes AbV5e AbV6. A adubação nitrogenada em cobertura foi realizada aos 20 dias após o transplantio das mudas na forma de ureia (45% de N).Os resultados reportaram que a aplicação de doses de N em coberturaresultou no incremento linear da altura de planta, diâmetro de colmo, número de folhas por planta, número de perfilhos e produção de matéria seca das plantas inoculadas com A. brasilense. Quando as plantas não foram inoculadas com A. brasilense, a adubação nitrogenada resultou apenas no aumento do número de folhas e de perfilhos por planta. O uso da inoculação de A. brasilenseem mudas de cana-de-açúcar pré-brotadas tem efeito benéfico no desenvolvimento das plantas somente quando associada a adubação nitrogenada em cobertura. As plantas de cana-de-açúcar não adubadas com fertilizante nitrogenado tem resposta negativa à inoculação com A. brasilense, indicando a ocorrência de possíveis efeitos fitotóxicos no desenvolvimento das plantas até os 60 dias após o transplantio.(AU)

A study was conducted with the objective of investigating the effects of the interaction between the inoculation of the pre-sprouted seedlings with Azospirillum brasilenseand the nitrogen fertilization in topdressing on the development of sugarcane plants(Saccharum officinarum). Pre-sprouted sugarcane seedlings, produced from 3.0 cm long mini-stems of the RB867515 variety, were transplanted 25 days after sprouting into 12 L plastic pots. The treatments were arranged in a randomized block design, in a 2 × 5 factorial scheme: two inoculation treatments [without (control) and inoculation with Azospirillum brasilense] and five application rates of N fertilizer in topdressing (0, 30, 60, 90 and 120 mg dm–3of N), with four repetitions. The inoculation with A. brasilense was carried out in transplanting the seedlings using 4 mL of the inoculum AzoTotal® per seedling, containing the strains AbV5 and AbV6. The nitrogen fertilization in topdressing was performed at 20 days after transplanting the seedlings as urea (45% of N). The results reported that the application of N fertilizer rates in topdressing resulted in a linear increase in plant height, stem diameter, number of leaves per plant, number of tillers and dry matter production of plants inoculated with A. brasilense. When the plants were not inoculated with A. brasilense, nitrogen fertilization resulted in an increase also in the number of leaves and tillers per plant. The use of A. brasilense inoculation in pre-sprouted sugarcane seedlings has a beneficial effect on the development of plants only when associated with nitrogen fertilization in topdressing. Sugarcane plants not fertilized with N have a negative response to inoculation with A. brasilense, indicating the occurrence of possible phytotoxic effects on plant development up to 60 days after transplanting.(AU)

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