RESUMO
Significant differences were found in the cyclic 3',5'-adenosine monophosphate (cAMP) levels in (-)-isoproterenol-stimulated rat erythrocytes. The BN strain had the highest level (13.1 +/- 1.29 pmol cAMP/10 mg Hb) and the LEW strain had the lowest cAMP level (3.29 +/- 1.76 pmol cAMP/10 mg Hb) in the erythrocytes. The high levels were inherited in three intercrosses in a dominant fashion. The results of the backcross breeding suggested diallelic inheritance. However, the polygenic effect was not ruled out.
Assuntos
Adenilil Ciclases/genética , AMP Cíclico/sangue , Eritrócitos/metabolismo , Isoproterenol/farmacologia , Polimorfismo Genético , 1-Metil-3-Isobutilxantina/farmacologia , Adenilil Ciclases/sangue , Animais , Eritrócitos/efeitos dos fármacos , Feminino , Masculino , Propranolol/farmacologia , Ratos , Ratos Endogâmicos ACI , Ratos Endogâmicos BN , Ratos Endogâmicos Lew , Ratos Endogâmicos , Especificidade da EspécieRESUMO
Six cases of microgranular variant acute promyelocytic leukemia (M3v) were studied by use of a multiparameter approach including morphology, cytochemistry, flow cytochemistry, flow cytometry, cytogenetics, and gene rearrangement. Three of six cases demonstrated both myeloid and monocytoid associated surface markers by flow cytometry. One of six cases had strong alpha-naphthyl-butyrate esterase (alpha-NBE) activity in addition to myeloperoxidase activity. There was no correlation between percentage of positive monocytoid surface markers and intensity of cytoplasmic alpha-NBE activity. Four of six cases also had a T-cell-associated surface antigen. Further studies indicated that the T-cell markers appeared to be on the promyelocytes and that the T-B receptor gene was not rearranged. Similarly, cytogenetics studies indicated only one clonal abnormality t(15q+; 17q-). Whether these cases represent true "lineage infidelity" remains to be answered. Future important studies are needed on normal hematopoietic progenitor cells at early stages of development and childhood to study lineage-specific characteristics and to determine whether co-expression normally exists during early development.
Assuntos
Leucemia Promielocítica Aguda/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticorpos Monoclonais , Antígenos de Diferenciação de Linfócitos T/análise , Células Sanguíneas/enzimologia , Células Sanguíneas/patologia , Citometria de Fluxo , Histocitoquímica , Humanos , Leucemia Promielocítica Aguda/enzimologia , Leucemia Promielocítica Aguda/imunologia , Leucemia Promielocítica Aguda/patologiaRESUMO
Commingling analysis of total leukocyte, neutrophil and lymphocyte concentrations in human blood suggested the effect of major regulatory factors affecting each type of white blood cells. Our results showed two distributions for the total leukocyte and lymphocyte concentrations. The values of the low and high phenotypes were 5.8 and 8.4 x 10(9)/l of total leukocytes and 2.0 and 4.6 x 10(9)/l of total lymphocytes. On the other hand, the neutrophil concentration in blood is probably regulated by equally penetrant factors. The means of the three phenotypes were 2.6, 4.2 and 6.5 x 10(9)/l. The evidence of two or three phenotypes, although consistent with the effect of a single-factor hypothesis (genetic or environmental), must be confirmed by segregation analysis of families.