RESUMO
Background: Metabolic capacity of gut microflora is huge and this microb organ can be considered as second biggestmetabolic organ in body. The potential for an antibiotic to influence gut microflora is related to its spectrum of activity,pharmacokinetics, dosage and length of administration. In terms of pharmacokinetics, the rate of intestinal absorption playsa fundamental role. Apart from basic physiological functions, bile acids and their analogues are recognized as transportpromoters for other substances, in potentiating their action. The aim of this study was to demonstrate potential protectiveeffect of monoketocholic bile acid on rat intestinal microflora from oral ampicillin.Materials, Methods & Results: Eighteen Wistar rats were divided into three groups (n = 6). The experimental protocol wasapproved by Ethics Committee on Animal Use of the University Novi Sad. All animals received 10 mL/kg of body weightof drugs solutions per os by oral intubations. The animals have been treated twice daily for three days, with saline, ampicillin 500 mg/kg and ampicillin 500 mg/kg + monoketocholic bile acid (MKH) 4 mg/kg. The fecal pellets were collectedtwice, before and after the treatment was completed. Within 2 h of collection, samples of whole pellets were processedmicrobiologically. Weighed portions of feces were suspended 1:10 in sterile 0.9% NaCl and further diluted with samesolutions up to 1: 1013. The number of colony forming units (CFU) was determined by direct counting. Only the platescontaining 30 to 300 CFU were considered as valid. The ampicillin treated group, showed significant reduction...(AU)
Assuntos
Animais , Ratos , Ácidos e Sais Biliares/administração & dosagem , Ácidos e Sais Biliares/efeitos adversos , Ácidos e Sais Biliares/análise , Microbioma Gastrointestinal/efeitos dos fármacos , Ampicilina/administração & dosagem , Ratos WistarRESUMO
Background: Metabolic capacity of gut microflora is huge and this microb organ can be considered as second biggestmetabolic organ in body. The potential for an antibiotic to influence gut microflora is related to its spectrum of activity,pharmacokinetics, dosage and length of administration. In terms of pharmacokinetics, the rate of intestinal absorption playsa fundamental role. Apart from basic physiological functions, bile acids and their analogues are recognized as transportpromoters for other substances, in potentiating their action. The aim of this study was to demonstrate potential protectiveeffect of monoketocholic bile acid on rat intestinal microflora from oral ampicillin.Materials, Methods & Results: Eighteen Wistar rats were divided into three groups (n = 6). The experimental protocol wasapproved by Ethics Committee on Animal Use of the University Novi Sad. All animals received 10 mL/kg of body weightof drugs solutions per os by oral intubations. The animals have been treated twice daily for three days, with saline, ampicillin 500 mg/kg and ampicillin 500 mg/kg + monoketocholic bile acid (MKH) 4 mg/kg. The fecal pellets were collectedtwice, before and after the treatment was completed. Within 2 h of collection, samples of whole pellets were processedmicrobiologically. Weighed portions of feces were suspended 1:10 in sterile 0.9% NaCl and further diluted with samesolutions up to 1: 1013. The number of colony forming units (CFU) was determined by direct counting. Only the platescontaining 30 to 300 CFU were considered as valid. The ampicillin treated group, showed significant reduction...