RESUMO
OBJECTIVE: To analyze the protein and messenger RNA (mRNA) expression of La/SSB, Ro/SSA 60, and Ro/SSA 52 antigens in minor salivary glands (MSG) from patients with primary Sjögren's syndrome (pSS). METHODS: La/SSB, Ro/SSA 60, and Ro/SSA 52 protein expression was studied by immunohistochemistry in MSG from 26 patients with pSS and 16 controls. mRNA expression was determined by real-time polymerase chain reaction in MSG of 10 patients with pSS and 7 controls. RESULTS: La/SSB and Ro/SSA 60, but not Ro/SSA 52, mRNA expression was higher in samples from patients with pSS compared to controls (p < 0.05). La/SSB protein had higher expression in the cytoplasm of ductal cells than in the cytoplasm of mucous acinar cells in patients with pSS (p = 0.013) but not in controls. Ro/SSA 60 had higher expression in the cytoplasm of ductal cells than in the cytoplasm of serous acinar cells in patients with pSS (p = 0.006) but not in controls. The Ro/SSA 52 expression pattern was similar in patients and controls. There was no association between circulating autoantibodies to Ro/SSA or La/SSB and the aberrant expression of the cognate autoantigens. CONCLUSION: The increased Ro/SSA 60 and La/SSB mRNA expression in MSG of patients with pSS as well as the differential Ro/SSA 60 and La/SSB protein expression in ductal cells of MSG in patients with pSS suggest that these these 2 autoantigens, but not Ro/SSA 52, are probably involved in triggering and maintaining the tissue-specific autoimmune response in pSS MSG and may contribute to the antigen-driven immune response and local autoantibody production in pSS.
Assuntos
Autoantígenos/metabolismo , RNA Mensageiro/metabolismo , RNA Citoplasmático Pequeno/metabolismo , Ribonucleoproteínas/metabolismo , Glândulas Salivares Menores/metabolismo , Síndrome de Sjogren/metabolismo , Adulto , Idoso , Autoantígenos/genética , Estudos de Casos e Controles , Citoplasma/metabolismo , Citoplasma/patologia , Feminino , Regulação da Expressão Gênica , Humanos , Masculino , Pessoa de Meia-Idade , RNA Mensageiro/genética , RNA Citoplasmático Pequeno/genética , Ribonucleoproteínas/genética , Glândulas Salivares Menores/patologia , Síndrome de Sjogren/genética , Síndrome de Sjogren/patologia , Antígeno SS-BRESUMO
Sessenta crianças com idade entre dois e doze anos - vinte com quadro de amigdalite aguda de repetiçäo e hipertrofia amigdaliana (Grupo I), vinte com amigdalite aguda de repetiçäo sem hipertrofia amigdaliana (Grupo II) e vinte com diagnóstico de hipertrofia amigdaliana sem amigdalite de repetiçäo (Grupo III) - foram submetidas à dosagem sérica de imunoglobulinas IgA, IgM e IgG e subclasses de IgG, para investigaçäo de imunodeficiência humoral. Os resultados evidenciaram apenas uma criança com diminuiçäo dos níveis séricos de IgA no Grupo I, sendo que as outras imunoglobulinas apresentaram-se normais em todos os grupos e faixas etárias