RESUMO

Probiotic bacteria and bioactive compounds obtained from plant origin stand out as ingredients with the potential to increase the healthiness of functional foods, as there is currently a recurrent search for them. Probiotics and bioactive compounds are sensitive to intrinsic and extrinsic factors in the processing and packaging of the finished product. In this sense, the present study aims to evaluate the co-encapsulation by spray dryer (inlet air temperature 120 °C, air flow 40 L / min, pressure of 0.6 MPa and 1.5 mm nozzle diameter) of probiotic bacteria (L.plantarum) and compounds extracted from red beet stems (betalains) in order to verify the interaction between both and achieve better viability and resistance of the encapsulated material. When studying the co-encapsulation of L.plantarum and betalains extracted from beet stems, an unexpected influence was observed with a decrease in probiotic viability in the highest concentration of extract (100 %), on the other hand, the concentration of 50 % was the best enabled and maintained the survival of L.plantarum in conditions of 25 °C (63.06 %), 8 °C (88.80 %) and -18 °C (89.28 %). The viability of the betalains and the probiotic was better preserved in storage at 8 and -18 °C, where the encapsulated stability for 120 days was successfully achieved. Thus, the polyfunctional formulation developed in this study proved to be promising, as it expands the possibilities of application and development of new foods.

Assuntos

RESUMO

Although blueberries are widely studied, little information exists on their composition and content of flavonol glycosides. Most studies identify only a few flavonols in blueberries due to separation and identification issues. In the present study, we identified 44 flavonols and chlorogenic acid in 30 samples of Highbush and Rabbiteye blueberry, using HPLC-DAD-ESI-MSn. Highbush group fruits presented mainly quercetin-3-galactoside in their composition, while Rabbiteye group fruits exhibited higher levels of quercetin-3-rhamnoside and quercetin-3-glucuronide. Among the identified flavonols, 8 acylates (acetyl and hydroxymethylglutaroyl) were found, of which quercetin-3-O-[4″-(3-hydroxy-3-methylglutaroyl)]-α-rhamnoside was found for the first time in blueberries. This compound is exclusive to the cultivars Florida and Powderblue, where it is present in high quantities. Glucuronides of syringetin and laricitrin, and rhamnosyl-galactosides of myricetin, quercetin and isorhamnetin were also found for the first time in blueberries. The Principal Component Analysis showed that blueberry groups can be distinguished based on their phenolic compound profile.

Assuntos

RESUMO

The fruits and vegetables are recommended for human feeding due to the richness of antioxidant compounds. The bioactive compounds from the diet, such as vitamins C and E are important to reduce the speed of initiation or prevent the spread of free radicals. The objective of the work was to evaluate the ascorbic acid and tocopherol content of selections of Pitanga (Eugenia uniflora L.), butiá (Butiá capitata), blackberry (Rubus spp), blueberry (Vaccium Reade ashei), loquat (Eribrotia japônica), jambolan (Eugenia jambolana), purple araçá (Psidium rufum), physalis (Physalis peruviana), pear (Pyrus communis) and peaches (Prunus persica). The fruits were from 2006/2007/2008 harvest, which were obtained in Pelotas (RS) region. The tocopherol analyses were performed according Rodrigues-Amaya (1999), and ascorbic acid according Vinci, Botre e Ruggieri (1995). The tocopherol and ascorbic acid identification and quantification was performed by high-efficiency liquid chromatography system (HPLC), using a fluorescent detector for tocopherol (excitation of 290 nm and emission of 330 nm) and a UV-Visible (254 nm) for ascorbic acid. The blackberry cv. Tupy showed the highest tocopherols content (8,251 ?g.g-1 fruit) when compared with the other fruit content. The tocopherol was not found in pears and peaches of cv. Sensação and cv. Granada. The ascorbic acid content ranged from 9,291 mg.g-1 fr

As frutas e as hortaliças são recomendadas na alimentação humana pela riqueza em compostos nutritivos e também pela presença de compostos que apresentam efeito antioxidante. Os compostos bioativos adquiridos através da dieta, como a vitamina C e a vitamina E, além do aspecto nutritivo, são importantes para reduzir a velocidade de iniciação ou prevenir a propagação de radicais livres. O objetivo deste estudo foi avaliar os teores de ácido ascórbico e de tocoferóis, de seleções de pitanga (Eugenia uniflora L.), butiá (Butiá capitata), amora-preta (Rubus spp), mirtilo (Vaccium ashei Reade), nêspera (Eribrotia japônica), jambolão (Eugenia jambolana), araçá roxo (Psidium rufum), physalis (Physalis peruviana), pêra (Pyrus communis) e pêssegos (Prunus persica). As frutas utilizadas foram das safras 2006/2007/2008, obtidas na região de Pelotas/RS. A análise dos tocoferóis foi realizada segundo Rodrigues-Amaya (1999), e do ácido ascórbico segundo Vinci, Botre e Ruggieri (1995). A identificação e quantificação dos tocoferóis e do ácido ascórbico foi realizada em um sistema de cromatografia liquída de alta eficiência (CLAE), utilizando detector de fluorescência para os tocoferóis (excitação em 290 nm e emissão em 330 nm) e detector de UV-Visível para o ácido ascórbico (254 nm). A amora-preta cv. Tupy apresentou maior teor de tocoferóis (8,251 µg.g-1 de fruta) quando comparado ao conteúdo