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Braz J Microbiol ; 47(3): 597-602, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27143037

RESUMO

In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a (+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3) strain rendered competent with CaCl2. Isopropyl ß-d-1-thiogalactopyranoside was used to induce the expression of cry1Ab in E. coli BL21(DE3), and consequently, ∼130kDa of Cry1Ab was obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000µgg(-1) caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. However, the mortality rates of Bt SY49-1 strains' spore-crystal mixture at the same dose were observed to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicated that cry1Ab may be considered as a good candidate in transgenic crop production and as an alternative biocontrol agent in controlling stored product moths.


Assuntos
Bacillus thuringiensis/fisiologia , Proteínas de Bactérias/genética , Endotoxinas/genética , Expressão Gênica , Proteínas Hemolisinas/genética , Controle de Insetos , Animais , Bacillus thuringiensis/ultraestrutura , Toxinas de Bacillus thuringiensis , Proteínas de Bactérias/metabolismo , Proteínas de Bactérias/toxicidade , Clonagem Molecular , Endotoxinas/metabolismo , Endotoxinas/toxicidade , Proteínas Hemolisinas/metabolismo , Proteínas Hemolisinas/toxicidade , Controle de Insetos/métodos , Inseticidas , Larva , Mariposas/efeitos dos fármacos
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