RESUMO
Splenic hemangiosarcoma (HSA) is a malignant tumor of endothelial cells that affects middle-aged and elderly dogs and is characterized by the formation of new blood vessels, commonly associated with necrotic and hemorrhagic areas. Despite its importance in veterinary medicine, few studies have identified markers with prognostic value for canine HSA. Thus, this study aimed to associate the clinicopathological findings (prostate-specific membrane antigen [PSMA], Claudin-5, and Ki67 gene and protein expression) with overall survival in HSA-affected patients. Fifty-three formalin-fixed and paraffin-embedded canine splenic HSA samples, previously diagnosed by histopathological examination, were used in this study. Claudin-5, PSMA, and Ki67 protein expression levels were evaluated by immunohistochemistry, and gene expression was evaluated by quantitative polymerase chain reaction. Claudin-5 protein overexpression was observed in patients with metastasis (p = 0.0078) and with stage III tumors compared to those with stage I and II tumors (p = 0.0451). In patients treated with surgery alone, low PSMA gene and protein expression (p = 0.05 and p = 0.0355, respectively) were associated with longer survival time. Longer survival time was observed in patients with a low Ki67 index (p = 0.0488). Our results indicate that Claudin-5 protein expression is associated with metastatic status, and PSMA gene and protein expression, and Ki67 index are associated with survival time.
RESUMO
Toxoplasma gondii, parasito do filo Apicomplexa e possui os felídeos como hospedeiros definitivos e aves e mamíferos como hospedeiros intermediários. Nos caprinos a toxoplasmose pode levar a abortos, natimortos, mumificação fetal e nascimento de crias debilitadas. O isolamento de parasitos viáveis de tecidos comestíveis, bem como de leite caprino revelam a importância desta espécie como fonte de infecção para os seres humanos. Assim, estudos são realizados na tentativa de descrever os fatores envolvidos na infecção por este protozoário. Este trabalho aborda aspectos clínicos e patológicos da toxoplasmose e faz uma revisão das características epidemiológicas favoráveis a infecção por T. gondii. (AU)
Toxoplasma gondii, parasite of the Apicomplexa phylum that has felids as definitive hosts and birds and mammals as intermediate hosts. In goats, toxoplasmosis can lead to abortion, stillbirths, fetal mummification and birth of weak goat kids. Isolation of viable parasites from edible tissues as well as goat milk reveals the importance of this species as a source of infection for humans. Thus, several studies have been carried out in an attempt to describe factors involved in the infection by this protozoan. This work addresses clinical and pathological aspects of toxoplasmosis and reviews the epidemiological characteristics favorable for T. gondii infection (AU)
Assuntos
Animais , Toxoplasmose , Fatores Epidemiológicos , Ruminantes , Manejo de EspécimesRESUMO
Toxoplasma gondii, parasito do filo Apicomplexa e possui os felídeos como hospedeiros definitivos e aves e mamíferos como hospedeiros intermediários. Nos caprinos a toxoplasmose pode levar a abortos, natimortos, mumificação fetal e nascimento de crias debilitadas. O isolamento de parasitos viáveis de tecidos comestíveis, bem como de leite caprino revelam a importância desta espécie como fonte de infecção para os seres humanos. Assim, estudos são realizados na tentativa de descrever os fatores envolvidos na infecção por este protozoário. Este trabalho aborda aspectos clínicos e patológicos da toxoplasmose e faz uma revisão das características epidemiológicas favoráveis a infecção por T. gondii.
Toxoplasma gondii, parasite of the Apicomplexa phylum that has felids as definitive hosts and birds and mammals as intermediate hosts. In goats, toxoplasmosis can lead to abortion, stillbirths, fetal mummification and birth of weak goat kids. Isolation of viable parasites from edible tissues as well as goat milk reveals the importance of this species as a source of infection for humans. Thus, several studies have been carried out in an attempt to describe factors involved in the infection by this protozoan. This work addresses clinical and pathological aspects of toxoplasmosis and reviews the epidemiological characteristics favorable for T. gondii infection