RESUMO
BACKGROUND: Previous reports related the presence of mouse mammary tumor virus (MMTV)-like gene sequences to human breast carcinoma. The aim of this study was to determine whether MMTV-like env gene sequences are present in breast cancer samples of Mexican women and in breast and lung cancer cell lines. METHODS: Using specific primers for MMTV, we tested 3 breast cancer cell lines, 4 non-small lung cancer cell lines and 119 breast cancer samples from Mexican women. RESULTS: MMTV-like gene sequences were amplified in the lung cancer cell INER-51, but not in the MCF-7 cell line that has been used as a positive control in other reports and in 5 of 119 (4.2%) breast cancer biopsy tissues. Furthermore, the identity of sequences of PCR products from INER-51 and a breast cancer-positive sample are 98 and 99% when compared with the env region of MMTV (GenBank accession No. AY161347). CONCLUSION: These results indicate that MMTV-like gene sequences are present in the Mexican population.
Assuntos
Neoplasias da Mama/virologia , Genes env , Vírus do Tumor Mamário do Camundongo/genética , Vírus do Tumor Mamário do Camundongo/isolamento & purificação , Neoplasias da Mama/patologia , Linhagem Celular , Primers do DNA , DNA de Neoplasias/análise , Feminino , Humanos , México , Dados de Sequência MolecularRESUMO
The prevalence of antibodies against human T-cell lymphotrophic virus (HTLV-I/II) in blood donors from the city of Monterrey, Mexico was investigated. We found that 4 out of 1017 sera (0.39%) reacted against HTLV-I/II, as determined by a passive agglutination test (PA). However, none of PA-positive sera reacted to HTLV-I/II specific polypeptides as demonstrated by Western blot. These findings indicate that the population of Monterrey has very low or no seroprevalence for HTLV-I/II.
Assuntos
Doadores de Sangue , Anticorpos Anti-HTLV-I/sangue , Infecções por HTLV-I/epidemiologia , Anticorpos Anti-HTLV-II/sangue , Infecções por HTLV-II/epidemiologia , Adolescente , Adulto , Feminino , Infecções por HTLV-I/sangue , Infecções por HTLV-II/sangue , Humanos , Masculino , México/epidemiologia , Pessoa de Meia-Idade , PrevalênciaRESUMO
HIV and HTLV-1 are retrovirus that can produce human disease. It is known that HTLV-1 is associated to the adult T cell leukemia and to the spastic tropical paraparesis. AIDS is now a pandemic infection and HTLV-1 has a high endemicity in the Caribbean region and Japan, whereas the south of the United States has a low endemicity. In Mexico there is little information on HTLV-1 incidence. In the present work we looked for anti HTLV-1 antibodies in one hundred persons that belong to the high risks AIDS population in the city of Monterrey, Mexico. We found that 93 sera were positive for anti HIV antibodies in a ELISA test and seven were negative. All 93 sera were also positive in the Western Blot assay. In the confirmatory test two out of the seven negative sera were classified as indeterminate and five as negative. We also included in this study 50 sera from healthy control volunteers that did not belong to the high risk AIDS population and resulted negative in the HIV and HTLV-1 test. Anti HTLV-1 antibodies were determined by using an agglutination test with gelatin particles covered with HTLV-1 and confirmed by a Western Blot assay. We found that only three sera resulted positive in this agglutination test, but were negative by the Western Blot technique.