RESUMO
To estimate the economic and financial viability of a pig farm in central sub-tropical Mexico within a 5-year planning horizon, a Monte Carlo simulation model was utilized. Net returns were projected using simulated values for the distribution of input and product processes, establishing 2021 as base scenario. A stochastic modelling approach was employed to determine the economic and financial outlook. The findings reveal a panorama of economic and financial viability. Net income increased by 555%, return on assets rose from 3.36% in 2022 to 11.34% in 2026, and the probability of decapitalization dropped from 58% to 13%, respectively in the aforesaid periods. Similarly, the probability of obtaining negative net income decreased from 40% in 2022 to 18% in 2026. The technological, productive, and economic management of the production unit allowed for a favorable scenario within the planning horizon. There is a growing interest in predicting the economic sectors worth investing in and supporting, considering their economic and development performance. This research offers both methodological and scientific evidence to demonstrate the feasibility of establishing a planning schedule and validating the suitability of the pork sector for public investment and support.
Assuntos
Fazendas , México , Animais , Suínos , Fazendas/economia , Modelos Econômicos , Criação de Animais Domésticos/economia , Método de Monte Carlo , Estudos Prospectivos , RendaRESUMO
The aim of this work was to determine endometrial mRNA expression and uterine protein localization of vascular endothelial growth factor (VEGF) and its receptors VEGFR1 and VEGFR2 during the estrous cycle and peri-implantation period in sows. Uterine tissues were collected from pregnant sows on days 12, 14, 16, and 18 after artificial insemination and from non-pregnant animals on days 2 and 12 of the estrous cycle (day 0 = day of estrus). Using immunohistochemistry, a positive signal for VEGF and its receptor VEGFR2 was found in uterine luminal epithelial cells, endometrial glands, stroma, blood vessels, and myometrium. A VEGFR1 signal was only found in endometrial and myometrial blood vessels and stroma. By day 18 of gestation, the mRNA expression levels of VEGF, VEGFR1, and VEGFR2 were higher than those observed on days 2 and 12 of the estrous cycle and on days 12, 14, and 16 of gestation. Then, a primary culture of sow endometrial epithelial cells was established to define the potential of the selective inhibition of VEGFR2 after treatment with inhibitor SU5416 and determine its effects on the expression pattern of the VEGF system. The endometrial epithelial cells treated with SU5416 showed a dose-dependent decrease in VEGFR1 and VEGFR2 mRNA expression. The present study provides additional evidence on the importance of the VEGF system during peri-implantation, as well as on the specific inhibitory activity of SU5416 in epithelial cells, which, as demonstrated, express the protein and mRNA of VEGF and its receptors VEGFR1 and VEGFR2.
Assuntos
Inibidores da Angiogênese , Fator A de Crescimento do Endotélio Vascular , Animais , Suínos , Feminino , Fator A de Crescimento do Endotélio Vascular/metabolismo , Inibidores da Angiogênese/metabolismo , Útero/metabolismo , Fatores de Crescimento do Endotélio Vascular/metabolismo , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/genética , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/metabolismo , RNA Mensageiro/metabolismo , Receptores de Fatores de Crescimento do Endotélio Vascular/metabolismoRESUMO
This study aimed to detect intracellular trehalose in boar sperm that were cryopreserved with liposomes and conduct an analysis of its effects on some characteristics of thawed sperm, including rheological properties. First, soybean lecithin cholesterol-based liposomes were produced and characterized in the presence of 300 mM trehalose. Next, semen samples were frozen in two freezing media: a control medium with 300 mM trehalose and an experimental medium supplemented with 300 mM trehalose and 10% liposomes, both of which were thawed and then studied to ascertain their integrity, motility, rheological response, and trehalose quantities by testing two methods of spermatic lysis via high-performance liquid chromatography with an evaporative light-scattering detector (HPLC-ELSD). The results found spherical liposomes measuring 357 nm that were relatively stable in an aqueous medium and had an entrapment efficiency of 73%. An analysis of the cryopreserved ejaculates showed that their viability and motility did not significantly differ between groups (P > 0.05). The viscous response of the samples was influenced by the extracellular medium rather than by the freezing-thawing process, which resulted in a loss of interaction between the cells and cryoprotectants. Finally, intracellular trehalose levels were determined using HPLC-ELSD, with no differences observed (P > 0.05) when comparing both sperm lysis methods. The use of liposomes with trehalose appears to be a promising option for boar semen cryopreservation, with a marked effect on rheological properties. The proposed HPLC-ELSD method was effective for measuring trehalose in cryopreserved cell samples.
Assuntos
Preservação do Sêmen , Sêmen , Masculino , Suínos , Animais , Sêmen/fisiologia , Trealose , Preservação do Sêmen/veterinária , Preservação do Sêmen/métodos , Lipossomos , Motilidade dos Espermatozoides/fisiologia , Dissacarídeos , Criopreservação/veterinária , Criopreservação/métodos , Crioprotetores/farmacologia , Espermatozoides/fisiologiaRESUMO
The Vietnamese pot-bellied pig is a breed with high investigation potential. However, at the reproductive level, its testicular characteristics are still unknown, as well as the different stages of its development. Therefore, the objective of this work is to describe the postnatal testicular development of Vietnamese pot-bellied pigs. In this study, we used pigs grouped into the neonatal stage, animals at zero weeks; prepubertal stage, animals at three and eight weeks; pubertal stage, animals at twelve and sixteen weeks; and postpubertal stage animals at twenty, twenty-four, twenty-eight and thirty-two weeks of age. The neonatal stage is characterized by gonocytes at different migration phases. In the prepubertal stage, gonocytes were differentiated into spermatogonia at 3 weeks of age, and the first spermatocytes were observed at 7 weeks of age. Puberty was determined to start at 12 weeks because seminiferous tubules are found with complete spermatogenesis and the highest peaks in positive cell counting of androgen receptors (AR) and proliferating cell nuclear antigen (PCNA) factor that later decreased and further stabilized in the following weeks. In the postpubertal stage, an increase in seminiferous tubule areas was observed. The number of apoptotic cells ranged from low to null at all ages. Testosterone (T) and gonadotropin levels had two important peaks at 3 and 24 weeks. The seminiferous epithelium cycle was found to have 11 stages according to acrosome development. These characteristics of Vietnamese pot-bellied pig testes, which are different from rat testes and more similar to human testicles, make them a viable model to study human male reproductive biology. The postnatal testicular development of pot-bellied pigs is different from the postnatal testicular development of other breeds. Therefore, due to this difference in size and easy manipulation, the Vietnamese pig is an alternative for investigation compared to other pig breeds.
Assuntos
Escroto/crescimento & desenvolvimento , Epitélio Seminífero/crescimento & desenvolvimento , Espermatogônias/crescimento & desenvolvimento , Testículo/crescimento & desenvolvimento , Animais , Animais Recém-Nascidos , Proliferação de Células , Humanos , Masculino , Modelos Animais , Antígeno Nuclear de Célula em Proliferação/metabolismo , Receptores Androgênicos , Túbulos Seminíferos/crescimento & desenvolvimento , Espermatogênese/fisiologia , Suínos , Fatores de TempoRESUMO
Swine enteric viral infections are responsible for substantial economic losses in the pork industry worldwide. Porcine epidemic diarrhea (PEDV) is one of the main causative agents of diarrhea in lactating pigs, and reports of PEDV coinfection with other enteric viruses highlight the importance of viral interactions for disease presentation and outcomes. Using next-generation sequencing (NGS) and sequence analyses from samples taken from piglets with acute diarrhea, we explored the possible interactions between PEDV and other less reported pathogens. PEDV coinfection with porcine kobuvirus (PKV) was detected in 36.4% (27/74) of samples. Full genomes from porcine coronavirus and kobuvirus were obtained, as was a partial porcine sapovirus genome (PSaV). The phylogenetic results show the clustering of these strains corresponding to the geographical relationship. To our knowledge, this is the first full genome and isolation report for porcine kobuvirus in México, as well as the first phylogenetic analysis for porcine sapovirus in the country. The NGS approach provides a better perspective of circulating viruses and other pathogens in affected production units.
Assuntos
Coinfecção/virologia , Infecções por Coronavirus/virologia , Kobuvirus/genética , Kobuvirus/isolamento & purificação , Vírus da Diarreia Epidêmica Suína/genética , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , Animais , Coinfecção/epidemiologia , Infecções por Coronavirus/epidemiologia , Diarreia/virologia , Fezes/virologia , Genoma Viral , Kobuvirus/classificação , México/epidemiologia , Técnicas de Diagnóstico Molecular , Filogenia , Vírus da Diarreia Epidêmica Suína/classificação , Sapovirus/genética , Análise de Sequência , Suínos , Doenças dos Suínos/virologiaRESUMO
INTRODUCTION AND OBJECTIVES: Cases of viral hepatitis reported in Mexico are typically identified as hepatitis A, B and C. However, unspecified cases are reported annually. Hepatitis E virus (HEV) is an emergent agent that causes a self-limiting infection that can evolve to chronic in immunosuppressed individuals. In Mexico, HEV genotype 2 is considered endemic, though it's the prevalence is not well known. Therefore, the present study was designed to determine the prevalence of HEV among patients at the "Hospital Infantil de Mexico Federico Gomez". MATERIALS AND METHODS: The study included 99 patients, anti-HEV antibody (IgG and IgM) were detected by indirect ELISA and viral genome was identified using RT-PCR technique. Two PCR products of positive cases were sequenced. RESULTS: ELISA results were positive in 3% and 6%, for IgG and IgM respectively, 54.5% prevalence was found by PCR. Low lymphocyte count (p<0.05) and malnutrition (p<0.005) were significant factors for high PCR prevalence and could increase the possibility of infection. Two samples were sequenced and confirmed the presence of HEV genotype 3. CONCLUSIONS: This report reveals the incidence of HEV in pediatric patients in Mexico. Moreover, the identification of HEV genotype 3 in human samples suggests a potential zoonotic risk that requires further research.
Assuntos
Vírus da Hepatite E/genética , Hepatite E/epidemiologia , Alanina Transaminase/sangue , Aspartato Aminotransferases/sangue , Bilirrubina/sangue , Criança , Estudos Transversais , DNA Viral/sangue , Feminino , Genoma Viral/genética , Genótipo , Hepatite A , Anticorpos Anti-Hepatite A/sangue , Anticorpos Anti-Hepatite , Anticorpos Anti-Hepatite B/sangue , Anticorpos Anti-Hepatite C/sangue , Hepatite E/sangue , Hepatite E/imunologia , Hepatite E/virologia , Humanos , Imunoglobulina G/imunologia , Imunoglobulina M/imunologia , Estudos Longitudinais , Masculino , México/epidemiologia , RNA Viral/sangue , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Estudos Soroepidemiológicos , Proteínas Virais/genéticaRESUMO
Influenza A viruses cause respiratory disease in piglets, and maternal immunity plays an important role in protecting against Influenza virus infection. Nevertheless, in the presence of high levels of maternal antibodies against influenza, an adequate immune response is not developed. In this study, the effect of maternal antibodies against the swine influenza A/swine/New Jersey/11/1976/H1N1 virus (swH1N1) on clinical presentation, serological response, and lesions produced in colostrated and non-colostrated pigs was evaluated in pigs infected with the human influenza A/Mexico/La Gloria-3/2009/ H1N1 (pH1N1) and swH1N1 viruses. Our results indicated that between 2 and 4 days post-challenge, sneezing and mild nasal discharge were observed in all pigs. Body temperature in pigs from all treatment groups ranged between 39.2 and 39.3 °C. Pigs inoculated with the pH1N1 virus (421 g) exhibited a significantly lower daily weight gain than those inoculated with the swH1N1 virus (524 g). HI antibody titers against the pH1N1 virus were significantly different between colostrated (1.62) and non-colostrated (0.43) pigs. Significant differences in antibody titers were detected between pigs inoculated with the pH1N1 (1.28) or the swH1N1 virus (0.77) (P < 0.05). The highest percentage of pulmonary lesions was observed in non-colostrated/pH1N1 pigs (11.88%) at 6 days post-challenge. Cross reactivity was observed between the pH1N1 and swH1N1 viruses, as the maternal antibodies against the swH1N1 virus successfully neutralized the pH1N1 virus infection.
RESUMO
BACKGROUND: Interest in porcine epidemic diarrhea has grown since the 2013 outbreak in the United States caused major losses, with mortality rates up to 100 % in suckling piglets. In Mexico, an outbreak of porcine epidemic diarrhea, characterized by 100 % mortality in piglets, began in March 2014 in the State of Mexico. METHODS: The aim of this study was to confirm and identify porcine epidemic diarrhea virus (PEDV) in samples from piglets with suggestive clinical signs using virological, histological, and molecular techniques. Necropsy was performed on 13 piglets from two litters with initial and advanced clinical signs. Suggestive lesions of acute infection with PEDV were detected in histological sections of the small and large bowels; specifically, multiple virus particles with visible crown-shaped projections were observed using electron microscopy and negative staining. Viral isolation was performed in Vero cells with trypsin. Infection was monitored by observation of cytopathic effect, and titration was determined by TCID50/ml. The presence of the PEDV in cultures and clinical samples was confirmed by RT-PCR amplification and sequencing of a 651-bp segment of the S glycoprotein gene, as well as a 681-bp matrix protein gene. RESULTS: The nucleotide sequence analysis of the Mexican isolates showed marked homology to viruses that circulated in 2013 in Colorado, USA. CONCLUSIONS: In this paper we confirm the isolation and characterization of PEDV from animals with early and advanced clinical signs.
Assuntos
Infecções por Coronavirus/veterinária , Surtos de Doenças/veterinária , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , Doenças dos Suínos/virologia , Animais , Chlorocebus aethiops , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/patologia , Infecções por Coronavirus/virologia , México/epidemiologia , Vírus da Diarreia Epidêmica Suína/ultraestrutura , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Suínos , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/patologia , Células VeroRESUMO
Hoy en día, las modernas técnicas de producción porcina exigen cada vez más destetes tempranos. Sin embrago, el destete representa una de las etapas más críticas en la vida productiva del cerdo, debido a que durante ella se suman una serie de factores estresantes y cambios fisiológicos. En la presente revisión se analizan los factores más importantes que causan estrés durante esta etapa, como la separación de la cerda, el transporte, el cambio de alimento, el alojamiento en nuevas instalaciones y el agrupamiento con lechones extraños. La interacción de los lechones con estos factores estresantes incrementa el nivel de estrés que representa, per se, la separación de la cerda y el lechón durante el destete, ya que habitualmente originan "retraso en el crecimiento", además de aumento de la susceptibilidad frente a agentes patógenos entéricos causantes de enfermedades. Por ello, los distintos factores que afectan la fisiología, el metabolismo y el comportamiento del lechón deben ser controlados adecuadamente. Se concluye que el conocimiento de la biología de la especie y un entrenamiento adecuado del personal, son necesarios para disminuir los problemas de bienestar del lechón destetado, por lo que, con el fin de evitarlos, se recomiendan algunas prácticas derivadas de los hallazgos presentados.
Nowadays, modern swine production techniques promote early weaning. Weaning constitutes one of the most critical stages in the productive life of swine as it is a phase that entails a whole series of stressful factors and physiological changes. This review examines the most important factors that cause stress during this stage, including: separation from the sow, transport, changes in alimentation, lodging in different installations, and being grouped together with strange piglets. The interaction of young pigs with all these stressor factors exercises a cumulative effect that intensifies the level of stress they experience during separation from the sow during the weaning process itself, an event that frequently results in "delayed growth" accompanied by an increased susceptibility to disease-causing, enteric pathogenic agents. For these reasons, it is important to adequately control the variety of factors that affect the physiology, metabolism and behavior of piglets. The conclusion is that both knowledge of the biology of the species and suitable personnel training are necessary to reduce the problems that arise from these issues related to the welfare of weaned piglets. Finally, this study recommends some practices derived from the findings presented, with a view to improving the welfare of recently weaned piglets.
RESUMO
Small-scale family swine production can be seen in thousands of small communities and suburban zones; it is characterized by breeding rural pigs in rustic facilities with basic feed and productive parameters, well below those of technologically advanced farms, with mortality reaching up to 50% of the litter. This type of production involves a large number of producers and has a critical impact on family income. At slaughter, these carcasses have a high probability of being seized because of the presence of cysticercoids (T. solium), losing a high percentage of its economic value; due to absence of slaughterhouses in rural communities, this meat is directed to consumption without health inspection, increasing the risk of transmission of this zoonosis. This paper describes the productive and reproductive characteristics of the backyard production system of pigs in a rural community. Human to pig ratio was 3:1. During the year, 308 piglets were born in 43 litters, 51.7% males and 48.3% females, with an average weight of 40.47 kg at 6 months and 65.30 kg at 10 months. They were slaughtered at 7.3 months on average. The highest death rate was found between 0 and 1 month of age. In many rural communities of Mexico pigs are fed with waste generated in homes, helping maintain the communities clean, as well as with the excreta of other species that usually roam freely, situation that can promote dissemination of parasitic diseases.
La porcicultura familiar es una forma de producción que existe en miles de pequeñas comunidades y zonas conurbadas, caracterizada por la crianza de razas criollas en instalaciones rústicas, con alimentación básica y parámetros productivos inferiores a los de las granjas tecnificadas y una mortalidad hasta de 50% de la camada. Este tipo de producción involucra gran número de productores e impacta críticamente en el ingreso familiar. Al sacrificio, estas canales tienen una alta probabilidad de decomiso por la presencia de cisticercosis (T. solium), por lo que pierden un alto porcentaje de su valor económico; debido a la ausencia de rastros en comunidades rurales, esta carne se destina al consumo directo, sin inspección sanitaria, incrementando los riesgos de transmisión de esta zoonosis. En este trabajo se describen las características productivas y reproductivas del sistema de producción de cerdos en traspatio en una comunidad rural. La razón humano-cerdo fue de 3:1, durante el año se obtuvieron 43 camadas, con 308 lechones nacidos vivos, 51.7% machos y 48.3% hembras, con un peso promedio de 40.47 kg a los 6 meses y 65.30 kg a los 10 meses, siendo sacrificados a los 7.3 meses en promedio, los animales que tienen entre 0 y 1 mes de edad presentan la tasa más alta de mortalidad sin que sean sacrificados. En muchas comunidades rurales de México, se alimentan con desperdicios que se generan en las casas, así como las excretas de otras especies que por lo regular deambulan libremente, situación que puede generar enfermedades parasitarias.