RESUMO
In the present work, liposomes have been used as nanocarriers in the biofortification of wheat plants with selenium (Se) through foliar application. Liposomal formulations were prepared using 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and Phospholipon®90H (P90H) (average size <100 nm), loaded with different concentrations of inorganic Se (selenite and selenate) and applied twice to the plants in the stage of vegetative growth. Liposomes enhanced Se uptake by wheat plants compared to direct application. The highest Se enrichment was achieved using the phospholipid DPPC and a concentration of 1000 µmol·L-1 of Se without affecting the biomass, chlorophylls, carotenoids, and the concentration of mineral nutrients of the plants. The chemical speciation of Se in the plants was further investigated by X-ray absorption spectroscopy (XAS). The results from XAS spectra revealed that most of the inorganic Se was transformed to organic Se and that the use of liposomes influenced the proportion of C-Se-C over C-Se-Se-C species.
Assuntos
Biofortificação , Lipossomos , Folhas de Planta , Selênio , Triticum , Triticum/química , Triticum/crescimento & desenvolvimento , Triticum/metabolismo , Lipossomos/química , Selênio/química , Selênio/metabolismo , Selênio/análise , Folhas de Planta/química , Folhas de Planta/metabolismo , Folhas de Planta/crescimento & desenvolvimento , Nanopartículas/química , Portadores de Fármacos/químicaRESUMO
Kale (Brassica oleracea L. var. sabellica L.), kohlrabi (Brassica oleracea L. var. gongylodes L.) and wheat (Triticum aestivum L. cv. Bancal) microgreens were cultivated in presence of selenium 20 µmol L-1 as sodium selenite and sodium selenate mixture. The influence of this biofortification process was evaluated in terms of biomass production, total Se, macro- and micronutrients concentration, polyphenols, antioxidant activity, chlorophylls and carotenoids levels and total soluble proteins content. The results obtained have shown a significant concentration of total Se in the biofortified microgreens of kale (133 µg Se·g-1 DW) and kohlrabi (127 µg Se·g-1 DW) higher than that obtained for wheat (28 µg Se·g-1 DW). The Se uptake in all the species did not produce oxidative damage to the plants reflected in the bioactive compounds, antioxidant capacity or pigments concentration. These Se-enriched microgreens may contribute to the recommended intake of this nutrient in human diet as to overcome Se-deficiency.
Assuntos
Brassica , Selênio , Humanos , Selênio/farmacologia , Selênio/metabolismo , Biofortificação/métodos , Antioxidantes/metabolismo , Brassica/metabolismo , Compostos Fitoquímicos/metabolismo , NutrientesRESUMO
Films made from neat chitosan and chitosan with magnetic nanoparticles (MNPs) were tested as adsorbents of arsenate ions. Sorption equilibrium and sorption kinetics studies are reported, including different models applied to enlighten experimental observations and predict results. The sorption of As (V) was reasonably explained using Freundlich isotherm for neat chitosan film although it was better represented by Langmuir equation for the composite sample. The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model. The adsorbent containing MNPs presented higher adsorption capacity, which was associated to the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface. For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.
Assuntos
Arsênio/isolamento & purificação , Quitosana/química , Poluentes Químicos da Água/isolamento & purificação , Purificação da Água/métodos , Adsorção/efeitos dos fármacos , Arsênio/toxicidade , Compostos Férricos/química , Cinética , Nanopartículas/química , Água/química , Poluentes Químicos da Água/toxicidadeRESUMO
This paper presents a fast and automatic flow-based method to extract 131I from biological samples and hospital waste, previous to liquid scintillation detection. 131I is a radionuclide extensively used in Nuclear Medicine due to their beta and gamma disintegrations, whereby hospitals have to manage the associated waste generation. The automatic developed system is based on Lab-On-Valve (LOV) flow-technique exploiting Cl-resin (135â¯mg per extraction). This methodology allows performing sample extractions and measurements on the same day, since the extraction frequency takes 1.4-4 h-1, depending on the analysed sample volume, plus up to 2â¯h of measurement for each vial. 131I is retained as iodine ion and eluted with sodium sulphide 0.2â¯molâ¯L-1. The maximum sample volume that can be preconcentrated is 20â¯mL, reaching an extraction efficiency of 85⯱â¯5%. The minimum detectable activity (MDA) is 0.05 Bq, showing a precision of 7% RSD (nâ¯=â¯5). Both, biological samples (urine and saliva) and hospital waste samples can be satisfactorily analysed by the proposed system, obtaining recoveries between 90 and 110%. The developed method is then suitable to implement in hospitals, improving the surveillance of the 131I environmental release.